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Mutations and intron polymorphisms in voltage-gated sodium channel genes of different geographic populations of Culex pipiens pallens/ Culex pipiens quinquefasciatus in China

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贫困所致传染病(英文) 2024年第2期13卷 6-16页

作者： Li Wenyu Ma Delong Mu Qunzheng Zhou Xinxin hua dongdong Zhao Chunchun Liu Qiyong Wang Jun Meng Fengxia State Key Laboratory of Infectious Disease Prevention and Control Department of Vector Biology and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention WHO Collaborating Centre for Vector Surveillance and Management Beijing People’s Republic of China Ningxia Center for Disease Prevention and Control YinChuan NingXia People’s Republic of China Jinan Shizhong Center for Disease Control and Prevention Jinan Shandong China Vanke School of Public Health Tsinghua University Beijing People’s Republic of China Weifang No. People’s Hospital Weifang Shandong People’s Republic of China Beijing Daxing Center for Disease Control and Prevention Beijing People’s Republic of China Jinan Second Maternal and Child Health Hospital Jinan Shandong China

Background Culex pipiens pallens andCulex pipiens quinquefasciatus are the dominant species ofCulex mosquitoes in China and important disease vectors. Long-term use of insecticides can cause mutations in the voltage-gated sodium channel (vgsc) gene of mosquitoes, but little is known about the current status and evolutionary origins ofvgsc gene in different geographic populations. Therefore, this study aimed to determine the current status ofvgsc genes inCx. p. pallens andCx. p. quinquefasciatus in China and to investigate the evolutionary inheritance of neighboring downstream introns of thevgsc gene to determine the impact of insecticides on long-term *** Sampling was conducted from July to September 2021 in representative habitats of 22 provincial-level administrative divisions in China. Genomic DNA was extracted from 1308 mosquitoes, the IIS6 fragment of thevgsc gene on the nerve cell membrane was amplified using polymerase chain reaction, and the sequence was used to evaluate allele frequency and knockdown resistance (kdr) frequency. MEGA 11 was used to construct neighborjoining (NJ) tree. PopART was used to build a TCS *** There were 6 alleles and 6 genotypes at the L1014 locus, which included the wild-type alleles TTA/L and CTA/L and the mutant alleles TTT/F, TTC/F, TCT/S and TCA/S. The geographic populations with akdr frequency less than 20.00% were mainly concentrated in the regions north of 38° N, and the geographic populations with akdr frequency greater than 80.00% were concentrated in the regions south of 30° *** frequency increased with decreasing latitude. And within the same latitude, the frequency ofkdr in large cities is relatively high. Mutations were correlated with the number of introns. The mutant allele TCA/S has only one intron, the mutant allele TTT/F has three introns, and the wild-type allele TTA/L has 17 *** Cx. p. pallens andCx. p. quinquefasciatus have developed resistance to insecticides in mos

关键词： Culex pipiens pallens Culex pipiens quinquefasciatus vgsc gene Mutation Frequency Intron Haplotype China

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A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology

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Infectious Diseases of Poverty 2023年第4期12卷 43-53页

作者： Qunzheng Mu Xin Zhao Fengfeng Li Wenyu Li Xinxin Zhou Xinchang Lun Yiguan Wang dongdong hua Qiyong Liu Di Xiao Fengxia Meng National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206People’s Republic of China Weifang No.2 People’s Hospital Weifang261000ShandongPeople’s Republic of China Weifang Medical College Weifang261000ShandongPeople’s Republic of China Beijing Daxing District Center for Disease Control and Prevention Beijing102600BeijingPeople’s Republic of China

Background The current prevention and control strategy forAedes albopictus heavily relies on comprehensive management, such as environmental management and chemical control. However, the wide application of pyrethroids has facilitated the development of insecticide resistance, primarily via mutations in the voltage-gated sodium channel (VGSC) gene. This study aims to develop a novel strategy for detecting mutations in the VGSC gene inAe. albopictus using multiplex PCR-mass spectrometry (MPCR-MS) minisequencing *** We established a new strategy for detecting mutations in the VGSC gene inAe. albopictus using MPCR-MS minisequencing technology. MPCR amplification and mass probe extension (MPE) were first used, followed by single nucleotide polymorphism (SNP) typing mass spectrometry, which allows the simultaneous detection of multiple mutation sites of the VGSC gene in 96 samples ofAe. albopictus. A total of 70 wild-collectedAe. albopictus were used to evaluate the performance of the method by comparing it with other *** Three target sites (1016, 1532, 1534) in the VGSC gene can be detected simultaneously by double PCR amplification combined with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, achieving a detection limit of 20 fg/μl. We applied this method to 70 wild-collectedAe. albopictus, and the obtained genotypes were consistent with the routine sequencing results, suggesting the accuracy of our *** MPCR-MS minisequencing technology provides a sensitive and high-throughput approach toAe. albopictus VGSC gene mutation screening. Compared with conventional sequencing, this method is economical and time-saving. It is of great value for insecticide resistance surveillance in areas with a high risk of vector-borne disease.

关键词： Aedes albopictus VGSC gene Mutation Single nucleotide polymorphisms Multiplex polymerase chain reaction-mass spectrometry mini-sequencing

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Inertinite in coal and its geoenvironmental significance:Insights from AI and big data analysis

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Science China Earth Sciences 2024年第6期67卷 1779-1801页

作者： Longyi SHAO Jiamin ZHOU Timothy PJONES Fanghui hua Xiaotao XU Zhiming YAN Haihai HOU dongdong WANG Jing LU State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources and College of Geoscience and Surveying Engineering China University of Miningand Technology(Beijing)Beijing 100083China School of Earth and Environmental Sciences Cardiff UniversityCardiff CF103YEWalesEngland General Prospecting Institute of China National Administration of Coal Geology Beijing 100039China Institute of Architectural Engineering Weifang UniversityWeifang 261061China College of Mining Liaoning Technical UniversityFuxin 123000China College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China

Inertinite,as an important and abundant maceral group in coal,is critical for the study of palaeowildfires and their roles in the Earth’s ***,there has been a significant amount of research on the relationship between palaeowildfire,palaeoclimate change and palaeovegetation evolution based on inertinite *** reflectance of fusinite and semifusinite has been used to estimate the combustion temperature and type of palaeowildfires,and then to evaluate the combustion characteristics of different types of *** relative abundance of inertinite can be used to estimate the atmospheric oxygen *** rapid development of artificial intelligence(AI)and online tools to search scientific databases has presented an opportunity for us to find,collect,arrange,and analyse data from the earliest to latest publications on *** data extraction tool Deep Shovel is used to collect and analyse global inertinite data from the Silurian to the *** software programs such as Gplates,ArcGIS pro and Tableau are then applied to model the relative abundance of inertinite over geological time,which can be correlated with other parameters such as atmospheric oxygen contents,plant evolution and palaeoclimate *** distribution of inertinite in coals varied over different geological periods,being typified by the“high inertinite content-high atmospheric oxygen level”period in the Permian and the“low inertinite content-low atmospheric oxygen level”period in the *** study has proposed a possible model of the positive and negative feedbacks between inertinite characteristics and palaeoenvironmental factors,and has revealed the exceptional role of inertinite in palaeoenvironmental *** research on inertinite will be focused on the integrated study of organic petrology and organic geochemistry of inertinite,the big data-driven research on the temporal and spatial distribution of the global inertinite,the exploration of the

关键词： Inertinite Palaeowildfires Atmospheric oxygen Palaeoclimates Palaeoecosystems

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建筑业“工程分包困局”初探——为什么要超越古典政治经济学?

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建筑经济 2022年第4期43卷 5-9页

作者：华冬冬亓霞沙凯逊山东建筑大学山东济南250014

以我国建筑业面临的“工程分包困局”为背景探析相关的理论问题。古典政治经济学的分工理论和比较优势理论可以很好地解释专业化分工的经济合理性,但是对“反命题”的解释能力不足。为了对这一困局作出合理的解释,需要引进交易成本的概... 详细信息

以我国建筑业面临的“工程分包困局”为背景探析相关的理论问题。古典政治经济学的分工理论和比较优势理论可以很好地解释专业化分工的经济合理性,但是对“反命题”的解释能力不足。为了对这一困局作出合理的解释,需要引进交易成本的概念。走出这一困局,不仅需要公共制序(政府规制和法律手段),而且需要私人制序(公司治理和项目治理)。

关键词：工程分包分工理论比较优势交易成本私人制序

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某中小跨径水泥混凝土桥面铺装改造加固与施工

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工程技术研究 2022年第6期7卷 64-66页

作者：华东东京珠高速公路广珠段有限公司广东中山528445

随着交通事业的蓬勃发展,高速公路桥梁桥面铺装层频繁出现裂缝、松散、坑槽等病害,维修周期也越来越短。文章针对20世纪90年代修建的某高速公路中小跨径连续刚构桥,结合桥面铺装病害现状,分析病害原因,提出桥面铺装改造设计方案和施工工... 详细信息

随着交通事业的蓬勃发展,高速公路桥梁桥面铺装层频繁出现裂缝、松散、坑槽等病害,维修周期也越来越短。文章针对20世纪90年代修建的某高速公路中小跨径连续刚构桥,结合桥面铺装病害现状,分析病害原因,提出桥面铺装改造设计方案和施工工艺,为桥面铺装的凿除重做施工提供了有益指导,取得了良好的应用效果。

关键词：桥面铺装铺装凿除病害施工工艺

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建筑业“工程分包困局”三探——“三尺之冰”是如何结成的?

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建筑经济 2022年第6期43卷 5-9页

作者：曾大林华冬冬沙凯逊山东建筑大学山东济南250014

从历史的角度出发,在行业层面上分析“工程分包困局”的成因。制度变迁过程具有路径依赖的特点。在本土市场缺席的条件下,鲁布革工程成功的主要原因就在于外来的市场机制和本土的科层组织在局部形成的混合优势。然而,计划经济体制的惯... 详细信息

从历史的角度出发,在行业层面上分析“工程分包困局”的成因。制度变迁过程具有路径依赖的特点。在本土市场缺席的条件下,鲁布革工程成功的主要原因就在于外来的市场机制和本土的科层组织在局部形成的混合优势。然而,计划经济体制的惯性作用不仅使早期的改革方案存在一些偏差,而且使后面的纠偏行动困难重重。这一困局本质上是帕累托无效的均衡状态,是我国经济转型期特定条件下的产物。

关键词：制度变迁路径依赖市场准入企业资质包工队

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Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes

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Science China(Life Sciences) 2024年第8期67卷 1697-1714页

作者： huan Wu Yanman Zhang Yuqian Li Shuya Sun Jintao Zhang Qingsong Xie Yue Dong Shushu Zhou Xuan Sha Kuokuo Li Jinyi Chen Xin Zhang Yang Gao Qunshan Shen Guanxiong Wang Xiaomin Zha Zongliu Duan dongdong Tang Chuan Xu Hao Geng Mingrong Lv Yuping Xu Ping Zhou Zhaolian Wei Rong hua Yunxia Cao Mingxi Liu Xiaojin He Reproductive Medicine Center Department of Obstetrics and GynecologyThe First Affiliated Hospital of Anhui Medical UniversityHefei 230022China State Key Laboratory of Reproductive Medicine and Offspring Health Department of Histology and EmbryologySchool of Basic Medical SciencesNanjing Medical UniversityNanjing 210029China State Key Laboratory of Reproductive Medicine and Offspring Health The Affiliated Taizhou People’s Hospital of Nanjing Medical UniversityTaizhou School of Clinical MedicineNanjing Medical UniversityNanjing 211166China Reproductive Medicine Center Department of Obstetrics and GynecologyShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China State Key Laboratory of Reproductive Medicine and Offspring Health Clinical Center of Reproductive MedicineThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract(Anhui Medical University) Hefei 230032China Key Laboratory of Population Health Across Life Cycle(Anhui Medical University) Ministry of Education of the People’s Republic of ChinaHefei 230032China Anhui Province Key Laboratory of Reproductive Health and Genetics Hefei 230032China Biopreservation and Artificial Organs Anhui Provincial Engineering Research CenterAnhui Medical UniversityHefei 230032China

The complexities of energy transfer mechanisms in the flagella of mammalian sperm flagella have been intensively investigated and demonstrate significant diversity across *** shuttles,particularly adenylate kinase(AK)and creatine kinase(CK),are pivotal in the efficient transfer of intracellular ATP,showing distinct tissue-and ***,the expression profiles of AK and CK were investigated in mice and found to fall into four subgroups,of which Subgroup III AKs were observed to be unique to the male reproductive system and conserved across *** AK8 and AK9 were found to be indispensable to male reproduction after analysis of an infertile male *** mouse models showed that AK8 and AK9 were central to promoting sperm *** combined with mass spectrometry revealed that AK8 and AK9 interact with the radial spoke(RS)of the *** of various human and mouse sperm samples with substructural damage,including the presence of multiple RS subunits,showed that the head of radial spoke 3 acts as an adapter for AK9 in the flagellar *** an ATP probe together with metabolomic analysis,it was found that AK8 and AK9 cooperatively regulated ATP transfer in the axoneme,and were concentrated at sites associated with energy consumption in the *** findings indicate a novel function for RS beyond its structural role,namely,the regulation of ATP *** conclusion,the results expand the functional spectrum of AK proteins and suggest a fresh model regarding ATP transfer within mammalian flagella.

关键词： adenylate kinase Subgroup III AKs AK8 AK9 male infertility ATP transfer radial spoke

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滚轮式PDC钻头工作机理的试验研究

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地下空间与工程学报 2024年第2期20卷 527-534,555页

作者：黄奎林李华宋东东王云田牛世伟西南石油大学机电工程学院成都610500 西南石油大学石油与天然气工程学院成都610500 中国石油集团川庆钻探工程有限公司川西钻探公司成都610057

针对页岩气钻采中定向钻进的PDC钻头扭矩波动大、冲击振动大、工具面不稳定等问题,笔者提出一种刀翼上设置有双侧支撑的滚轮新结构钻头,通过滚轮单元破岩实验以及全钻头钻进破岩实验来开展新型钻头减扭、缓冲机理研究,主要研究6种滚轮... 详细信息

针对页岩气钻采中定向钻进的PDC钻头扭矩波动大、冲击振动大、工具面不稳定等问题,笔者提出一种刀翼上设置有双侧支撑的滚轮新结构钻头,通过滚轮单元破岩实验以及全钻头钻进破岩实验来开展新型钻头减扭、缓冲机理研究,主要研究6种滚轮轮廓形状、4种滚轮的吃入深度、6种滚轮径向位置、7种PDC齿相对高度等因素对新型钻头扭矩、钻压的影响规律。结果表明:6种轮廓滚轮的等效摩擦系数均低于PDC齿,且为PDC齿的18.65%~43.73%,得出滚轮减扭能力高于PDC齿;6种不同径向位置的滚轮缓冲能力均强于PDC齿,为PDC齿的1.59~9.17倍,滚轮式PDC钻头的扭矩为常规PDC钻头的71.72%~98.33%;随着滚轮与PDC齿相对高度的减小,钻头扭矩逐渐减小,滚轮钻压的占比也逐渐增加,钻头减扭能力、缓冲能力均增加,为适用于页岩气钻采的新型钻头产品设计与研制提供理论支撑。

关键词：滚轮 PDC钻头减扭机理减振机理试验研究

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基层全科医生糖尿病微血管病变知信行评价量表的信效度检验

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中华全科医师杂志 2024年第5期23卷 463-468页

作者：兰成佃黄翠玲陈章燕陈冬冬祝墡珠杨华复旦大学附属中山医院(厦门)全科医学科厦门361015 上海市徐汇区华泾镇社区卫生服务中心上海200231 复旦大学附属中山医院全科医学科上海200032

目的评估“基层全科医生糖尿病微血管病变知信行评价量表”的信度和效度。方法该研究为横断面研究。2023年3—5月,采用“基层全科医生糖尿病微血管病变知信行量表”对上海4个行政区55家社区卫生服务中心的全科医生进行电子问卷调查。4周... 详细信息

目的评估“基层全科医生糖尿病微血管病变知信行评价量表”的信度和效度。方法该研究为横断面研究。2023年3—5月,采用“基层全科医生糖尿病微血管病变知信行量表”对上海4个行政区55家社区卫生服务中心的全科医生进行电子问卷调查。4周后,采用随机数字表法抽样选取30名调查对象对量表进行重复调查。以内容效度、结构效度、区分效度检验量表的效度;以内部一致性信度和重测信度检验量表的信度。结果本次调查共回收693份问卷,有效问卷661份,有效回收率95.4%。其中男性191名(28.90%),年龄(39.38±7.99)岁,工作年限(10.15±5.99)年。在内容效度方面,Pearson相关分析显示各分量表与总量表呈正相关,相关系数为0.490~0.876,均P0.3,量表具有较好的结构效度,验证性因子分析显示修订后的量表卡方自由度比(χ^(²)/df)=2.729、比较拟合指数(CFI)=0.784、近似误差方根(RMSEA)=0.072,模型拟合度好;区分效度方面,高分组与低分组在总量表和分量表得分上的差异均具有统计学意义(t=10.82~44.49,均P0.05)。结论修订后的“基层全科医生糖尿病微血管病变知信行评价量表”信效度较好,可用作基层全科医生糖尿病微血管病变管理的评价工具。

关键词：糖尿病微血管病变量表信度效度全科医生

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一个建设项目垂直治理的委托代理模型

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项目管理技术 2011年第5期9卷 28-34页

作者：沙凯逊华冬冬徐聪山东建筑大学管理工程学院

采用基于新制度经济学的研究范式，把建设项目看做是对不确定性的一种适应机制。从建设项目的特殊性出发，对威廉姆森在治理结构分析中所采用的简化处理方法做出修正，提出“二阶转变”的假设：当信息缺口达到一定程度，并且专用知识的... 详细信息

采用基于新制度经济学的研究范式，把建设项目看做是对不确定性的一种适应机制。从建设项目的特殊性出发，对威廉姆森在治理结构分析中所采用的简化处理方法做出修正，提出“二阶转变”的假设：当信息缺口达到一定程度，并且专用知识的比重超过一定程度时，建设项目治理结构的选择过程有可能不再沿着经典理论的预期方向发展，而是发生转折。将不确定性和信息成本纳入分析框架，构建委托代理模型分析建设项目的采购方式和契约类型。静态比较分析的结果表明，信息缺口和信息的相对成本系数是决定建设项目治理结构和报酬结构的重要因素，它们不仅决定“二阶转变”发生的时机，而且决定“弱激励区”的分布。

关键词：建设项目不确定性信息成本采购方式契约类型

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