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Virologica Sinica 2015年第3期30卷 190-199页

作者： Junwen Zheng Pu Yang Yan Tang dongchi zhao Department of Pediatrics Zhongnan Hospital of Wuhan University Department of Anatomy School of Medicine Wuhan University

HEp-2 cells persistently infected with respiratory syncytial virus(RSV) are a heterogeneous mixture of viral antigen-positive and-negative variants; however, the mechanism through which viral replication becomes latent remains unclear. In this study, we investigated the potential mechanism by which RSV escapes from innate immune surveillance. Persistent-infected RSV HEp-2 cells were isolated and cell clones were passaged. The RSV-persistent cells produced viruses at a lower titer, resisted wild-type RSV re-infection, and secreted high levels of interferon-β(IFN-β), macrophage inflammatory protein-1α(Mip-1α), interleukin-8(IL-8), and Rantes. Toll-like receptor 3(TLR3), retinoic acid inducible gene-I(RIG-I), and suppressor of cytokine signaling 1(SOCS1) levels were upregulated in these cells. The silencing of TLR3 m RNA decreased the expression of SOCS1 protein and the secretion of cytokines. RSV-persistent cells are in an inflammatory state; upregulation of SOCS1 is related to the TLR3 signaling pathway, which could be associated with the mechanism of viral persistence.

关键词： respiratory syncytial virus(RSV) suppressor of cytokine signaling 1(SOCS1) interferon toll-like receptor 3(TLR3) RSV-persistent cell line

Recent Advances for Global Perspectives on Etiology, Pathophysiology, Clinical Presentations, and Management of Moyamoya Disease

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World Journal of Neuroscience 2024年第1期14卷 6-23页

作者： Maiko Charles Mkwambe dongchi zhao Youping Deng Department of Pediatrics Zhongnan Hospital of Wuhan University Wuhan China

Moyamoya disease (MMD) is a condition characterized by the gradual narrowing and blockage of blood vessels in the brain, specifically those in the circle of Willis and the arteries that supply it. This results in reduced blood flow and oxygen to the brain, leading to progressive symptoms and potential complications. The underlying pathophysiological mechanism remains elucidated. However, recent studies have highlighted numerous etiologic factors: abnormal immune complex responses, susceptibility genes, branched-chain amino acids, antibodies, heritable diseases, and acquired diseases, which may be the great potential triggers for the development of moyamoya disease. Its clinical presentation has varying degrees from transient asymptomatic events to significant neurological deficits. Moyamoya disease (MMD) shows different patterns in children and adults. Children with MMD are more susceptible to ischemic events due to decreased blood flow to the brain. Conversely, adults with MMD are more prone to hemorrhagic events involving brain bleeding. Children with MMD may experience a range of symptoms including motor impairments, sensory issues, seizures, headaches, dizziness, cognitive delays, or ongoing neurological problems. Although adults may present with similar clinical symptoms as children, they are more prone to experiencing sudden onset intraventricular, subarachnoid, or intracerebral hemorrhages. One of the challenges in moyamoya disease is the potential for misdiagnosis or delayed diagnosis, particularly when physicians fail to consider MMD as a possible cause in stroke patients. This review aims to provide a comprehensive overview of recent global studies on the pathophysiology of MMD, along with advancements in its management. Additionally, the review will delve into various surgical treatment options for MMD, as well as its rare occurrence alongside atrioventricular malformations. Exciting prospects include the use of autologous bone marrow transplant and the p

关键词： Moyamoya Disease (MMD) Etiology Pathophysiology Clinical Presentations Management Future Promising Avenues

Review on Hydroxyurea Usage in Young Children with Sickle Cell Disease: Examining Hemoglobin Induction, Potential Benefits, Responses, Safety, and Effectiveness

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International Journal of Clinical Medicine 2024年第1期15卷 1-18页

作者： Maiko Charles Mkwambe Youping Deng dongchi zhao Department of Pediatrics Zhongnan Hospital of Wuhan University Wuhan China

Sickle cell disease (SCD) is a prevalent condition, particularly in the countries of sub-Saharan Africa, where the presence of specific genes associated with Malaria contributes to its high prevalence. Patients with sickle cell disease frequently experience painful episodes necessitating hospitalization, and their hemoglobin levels are typically lower than those of the general population. There are different treatment options available to manage complications, such as transfusing blood, hydroxyurea, and strong anti-pains. However, with all these treatments, patients still commonly experience pain crises and suffer from organ damage. Hydroxyurea, the sole approved medication for sickle cell anemia in developed and developing countries, is widely used in children despite being primarily indicated for adults. Multiple studies have demonstrated the efficacy of hydroxyurea in inducing HbF production in young children with SCD. Elevated HbF levels have been associated with improved clinical outcomes, including a reduction in vaso-occlusive crises, acute chest syndrome, and the need for blood transfusions. Furthermore, increased HbF levels have been shown to ameliorate disease-related organ damage, such as pulmonary hypertension and sickle cell retinopathy. The response to hydroxyurea treatment in young children with SCD is variable. Some patients achieve substantial increases in HbF levels and experience significant clinical benefits, while others may have a more modest response. Factors influencing the response include baseline HbF levels, genetic modifiers, treatment adherence, and dose optimization. Safety is a crucial consideration when using hydroxyurea in young children. Studies have shown that hydroxyurea is generally well-tolerated, with the most common adverse effects being myelosuppression, gastrointestinal symptoms, and dermatological manifestations. However,long-term effects and potential risks, such as renal dysfunction and reproductive impacts, require furth

关键词： Effectiveness Hydroxyurea Sickle Cell Disease Sickle Cell Anemia Minimally Effective Dose Maximum Tolerated Dose

Association between Supraventricular Tachycardia and Necrotizing Enterocolitis: A Case-Control Study

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Open Journal of Pediatrics 2022年第5期12卷 793-801页

作者： Ali Omar Jimale Zakaria Ahmed Mohamed dongchi zhao Department of Pediatrics Zhongnan Hospital of Wuhan University Wuhan China

Background: Necrotizing enterocolitis (NEC) is the most common and fatal gastrointestinal disease encountered in the Neonatal Intensive Care Unit. Several case reports have shown an association between supraventricular tachycardia and necrotizing enterocolitis. This study aimed to determine the association between supraventricular tachycardia and necrotizing enterocolitis. Methods: This study was conducted from April 1st, 2016 to March 31st, 2022, at the Department of Pediatrics, Zhongnan Hospital of Wuhan University, Hubei, China. The records of 74 subjects with the diagnosis of necrotizing enterocolitis (NEC) were obtained from the hospital’s medical data records. Consequently, 74 gender, gestational age, and birth weight-matched controls (babies without NEC) were recruited as controls. Results: Of the 74 cases, 47.3% of the cases were males, and 52.7% were females. Regarding the birth weight and gestational age, 77% of the cases had low birth weight (LBW) and 86.5% were premature. In terms of Apgar score, 93.2% of NEC cases had an Apgar score of >7 at five minutes. The median values of white blood cells, platelets, and hemoglobin of cases were 10.90 (8.09, 13.80), 227 (169.75, 295.50), and 155.6 (130.53, 170.95), respectively. No Association between supraventricular tachycardia and necrotizing enterocolitis (P = 1.00). Conclusion: No association between necrotizing enterocolitis and supraventricular tachycardia was found. Further multicenter-based studies examining whether there is a potential relationship exists between supraventricular tachycardia and the development of necrotizing enterocolitis are required.

关键词： Supraventricular Tachycardia Neonatal Arrhythmias Necrotizing Enterocol-itis

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新生儿败血症的抗菌药物选择与使用策略

中华儿科杂志 2022年第6期60卷 612-614页

作者：赵东赤武汉大学中南医院妇儿医院儿科武汉430071

新生儿是感染高风险人群,细菌性败血症是新生儿重症监护病房收治的常见感染性疾病和重要的致死原因。新生儿败血症常缺乏特异性表现,恰当使用抗菌药物对成功救治意义重大。本文简要介绍新生儿败血症的抗菌药物选择与使用策略,以对临床... 详细信息

新生儿是感染高风险人群,细菌性败血症是新生儿重症监护病房收治的常见感染性疾病和重要的致死原因。新生儿败血症常缺乏特异性表现,恰当使用抗菌药物对成功救治意义重大。本文简要介绍新生儿败血症的抗菌药物选择与使用策略,以对临床工作有所帮助。

关键词：新生儿败血症新生儿重症监护病房高风险人群抗菌药物选择使用策略感染性疾病细菌性败血症致死原因

Diagnosis, treatment, and prevention of 2019 novel coronavirus infection in children: experts' consensus statement

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World Journal of Pediatrics 2020年第3期16卷 223-231页

作者： Kunling Shen Yonghong Yang Tianyou Wang dongchi zhao Yi Jiang Runming Jin Yuejie Zheng Baoping Xu Zhengde Xie Likai Lin Yunxiao Shang Xiaoxia Lu Sainan Shu Yan Bai Jikui Deng Min Lu Leping Ye Xuefeng Wang Yongyan Wang Liwei Gao Global Pediatric Pulmonology Alliance Department of Respiratory Medicine Beijing Children's HospitalCapital Medical UniversityBeijingChina Beijing Pediatric Research Institute Beijing Children's HospitalCapital Medical UniversityBeijingChina Center of Hematologic Oncology Beijing Children's HospitalCapital Medical UniversityBeijingChina Department of Pediatrics Zhongnan Hospital of Wuhan UniversityWuhanChina Department of Pediatrics Renmin Hospital of Wuhan UniversityWuhanChina Department of Pediatrics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Respiratory Medicine Shenzhen Children's HospitalShenzhenChina Hospital Management Institute of Wuhan University Zhongnan Hospital of Wuhan UniversityWuhanChina Department of Pediatric Respiratory Shengjing Hospital of China Medical UniversityShenyangChina Department of Respiratory Medicine Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Infectious Disease Shenzhen Children's HospitalShenzhenChina Department of Respiratory Medicine Children's Hospital of ShanghaiShanghaiChina Department of Pediatrics Peking University First HospitalBeijingChina Department of Pediatrics Affiliated Hospital of Liaoning University of Traditional Chinese MedicineShenyangChina Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical SciencesBeijingChina

Since the outbreak of 2019 novel coronavirus infection (2019-nCoV) in Wuhan City,China,by January 30,2020,a total of 9692 confirmed cases and 15,238 suspected cases have been reported around 31 provinces or cities in *** the confirmed cases,1527 were severe cases,171 had recovered and been discharged at home,and 213 *** among these cases,a total of 28 children aged from 1 month to 17 years have been reported in *** standardizing prevention and management of 2019-nCoV infections in children,we called up an experts' committee to formulate this experts' consensus *** statement is based on the Novel Coronavirus Infection Pneumonia Diagnosis and Treatment Standards (the fourth edition) (National Health Committee) and other previous diagnosis and treatment strategies for pediatric virus *** present consensus statement summarizes current strategies on diagnosis,treatment,and prevention of 2019-nCoV infection in children.

关键词： prevention diagnosis treatment

呼吸道合胞病毒对上皮细胞TLR3/4和SOCS1/3表达的影响

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武汉大学学报（医学版） 2009年第5期30卷 579-582页

作者：李丹赵东赤杨坤武汉大学中南医院儿科湖北武汉430071

目的:探讨呼吸道合胞病毒(RSV)感染上皮细胞中抗病毒信号激活以及细胞因子负调节系统对细胞因子分泌的影响。方法:应用ELISA法检测RSV感染人喉癌上皮细胞(Hep2)培养上清中0,1,2,4,8,12,24 h干扰素α(IFNα-)的浓度;RT-PCR检测病毒感染... 详细信息

目的:探讨呼吸道合胞病毒(RSV)感染上皮细胞中抗病毒信号激活以及细胞因子负调节系统对细胞因子分泌的影响。方法:应用ELISA法检测RSV感染人喉癌上皮细胞(Hep2)培养上清中0,1,2,4,8,12,24 h干扰素α(IFNα-)的浓度;RT-PCR检测病毒感染后各时段上皮细胞内细胞因子信号传导抑制因子(SOCS)1,3和Toll样受体(TLR)3,4的mRNA表达水平。结果:RSV感染上调Hep2细胞SOCS3 mRNA的表达(P<0.05),SOCS1的表达先增高后减低,但无统计学意义;TLR3,TLR4 mRNA的表达增高表现为时间依赖性(P<0.05);IFNβ-的表达在RSV感染后短暂升高而后迅速降低并低于基础表达量(P<0.05)。结论:RSV感染Hep2细胞后上调TLR3,TLR4和SOCS3 mRNA的表达,抑制IFN-α的分泌,提示病毒复制可能通过上调SOCS3而拮抗TLR途径激活的宿主抗病毒免疫应答。

关键词：呼吸道合胞病毒 Hep2细胞干扰素-α Toll样受体细胞因子信号传导抑制因子

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热性惊厥儿童IMPA2基因多态性分析

武汉大学学报（医学版） 2010年第2期31卷 179-181,185页

作者：刘新旻赵峰赵东赤王大斌郧阳医学院附属太和医院儿童医疗中心湖北十堰442000 武汉大学中南医院儿科湖北武汉430071

目的:分析IMPA2基因多态性与儿童热性惊厥易感性的关系。方法:采用病例-对照研究方法和聚合酶链反应-限制片断长度多态分析技术(PCR-RFLP),检测热性惊厥病例组和健康对照组的IMPA2基因多态性。结果:IMPA2基因单核甘酸多态性位点159T>C... 详细信息

目的:分析IMPA2基因多态性与儿童热性惊厥易感性的关系。方法:采用病例-对照研究方法和聚合酶链反应-限制片断长度多态分析技术(PCR-RFLP),检测热性惊厥病例组和健康对照组的IMPA2基因多态性。结果:IMPA2基因单核甘酸多态性位点159T>C病例组与对照组基因型分布无显著差异,而单核甘酸多态性位点558C>T病例组与对照组基因型分布有显著差异(PT可能是热性惊厥的多基因遗传位点之一。

关键词：热性惊厥 IMPA2 单核苷酸多态性

The Prevalence and Clinical Manifestations of Co-Infection in Pediatric Infectious Mononucleosis: A Single-Centered, Retrospective Study

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Open Journal of Pediatrics 2024年第3期14卷 459-475页

作者： Abdishakur Abdukadir Muse Zakaria Ahmed Mohamed Pu Yang Lihong Liao Bibek Dhar Shrestha Agness Nicholaus Kanusya Paul Mwale Youping Deng dongchi zhao Department of Pediatrics Zhongnan Hospital of Wuhan University Wuhan China Department of Ophthalmology Zhongnan Hospital of Wuhan University Wuhan China

Background: Recent studies indicate that the incidence of infectious mononucleosis (IM) has increased in China. Furthermore, it has been shown that children diagnosed with IM are prone to acquiring other pathogens. However, there is limited research on the prevalence of these co-infections in children with IM. Thus, we conducted this study to determine the prevalence of coinfections and common pathogens, as well as to compare clinical manifestations in children with and without coinfections. Methods: This retrospective observational study was conducted at the Department of Pediatrics Zhongnan Hospital of Wuhan University, Wuhan, China, with data from January 2018 to January 2023. Data, including demographics, symptoms, lab results, and complications, were collected from the hospital’s electronic database and analyzed. The statistical analysis included descriptive statistics, independent samples t-tests and Mann-Whitney tests to compare the means of continuous variables. Statistical significance was determined by p-values less than 0.05. Results: The study involved 216 participants diagnosed with IM, predominantly males (61.6%) aged 0 - 4 years (50.9%). Coinfection was detected in 39.8% of children, with multiple pathogens present in 33.72% of these cases. Among coinfection cases, 40% occurred in children under 5 years old, and females made up 54.2% of these cases. Mycoplasma pneumoniae (MP) was the most prevalent pathogen, accounting for 18.1% of cases. Influenza B (IFB) and Influenza A (IFA) viruses were found in 16.7% and 13.9% of participants, respectively, indicating a notable occurrence of respiratory pathogen coinfections. Male gender, fever, tonsillopharyngitis, lower HGB levels, higher ESR, CRP, and AST levels were correlated with coinfections. Conclusion: In summary, the study revealed a high prevalence of coinfections among children diagnosed with IM, particularly involving Mycoplasma pneumoniae and influenza viruses. These coinfections were notably

关键词： Prevalence Mononucleosis Coinfection Children Epstein-Barr Virus Features

精氨酸-甘氨酸-天冬氨酸肽修饰的脂质体雷公藤甲素靶向抗肿瘤效果

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中华实验外科杂志 2015年第3期32卷 505-507页

作者：熊小丽陈鹏赵东赤武汉大学中南医院儿科 430071 武汉市儿童医院中西医结合科武汉市儿童医院中西医结合科武汉大学中南医院儿科 430071

目的制备精氨酸-甘氨酸-天冬氨酸（RGD）肽修饰的脂质体雷公藤甲素,探讨RGD肽靶向脂质体雷公藤甲素（RGD-TPL-LPs）纳米药物在小鼠移植性肝癌模型的靶向效果及药效.方法通过成膜法制备具有靶向作用的脂质体纳米药物（RGD-TPL-LPs）,... 详细信息

目的制备精氨酸-甘氨酸-天冬氨酸（RGD）肽修饰的脂质体雷公藤甲素,探讨RGD肽靶向脂质体雷公藤甲素（RGD-TPL-LPs）纳米药物在小鼠移植性肝癌模型的靶向效果及药效.方法通过成膜法制备具有靶向作用的脂质体纳米药物（RGD-TPL-LPs）,并观察细胞毒性.建立小鼠移植肝癌肿瘤模型并评价药效.结果靶向修饰脂质体RGD-TPL-LPs的肿瘤细胞毒性明显强于无靶向脂质体（TPL-LPs）.此外,RGD靶向修饰的RGD-TPL-LPs抑瘤率为61.2％,要明显高于无靶向TPL-LPs的45.2％和游离雷公藤甲素的20.0％.结论 RGD靶向修饰的雷公藤甲素脂质体能够明显抑制肿瘤的生长,具有显著的抗肿瘤效果.

关键词：精氨酸-甘氨酸-天冬氨酸脂质体雷公藤甲素癌,肝细胞