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In-vitro Developmental Potential of Single Blastomeres Derived from Day 3 Discarded Human Embryos

Journal of Reproduction and Contraception 2010年第1期21卷 1-8页

作者： dong-mei ji yi-juan sun ai-jun zhang xiao-wei lu zhi-hong niu yun feng Reproductive Medical Center Ruijin Hospital Shanghai Jiao Tong University of Medicine Shanghai 200025 China

Objective To investigate whether isolated blastomeres of discarded human embryos could develop into blastocysts cultured in vitro without zona pellucida. Methods Total discarded 60 embryos, which were not suitable for transplanting or freezing, were collected from 21 patients. Of 60 embryos, 10, 8, 24, 12, and 4 embryos were at 2-, 3-, 4-, 5- and 6-cell stage, respectively. These embryos were split by 0.5% protease combined with mechanical method. The resulting single blastomere was cultured individually, evaluated daily and counted blastocyst development. Pluripotency of inner cell mass （ICM） of the blastocyst was analyzed by the expression of alkaline phosphatase（AKP）. Results A total of 229 single blastomeres were isolated from 60 embryos. Defining the day when the embryos were split as the first day （d 1）. The majority of the blastomere- derived embryos followed the normal pattern of development with compaction on d 3 and cavitationon d 4 and developed into small blastocysts on d 5. Rates of division, compaction, cavity and expansion of single blastomeres from 4-cell embryos were higher than those in other groups （P 〈O.05）, and AKP was expressed in ICM. Conclusion Some of the blastomeres from discarded human embryo are flexible and able to develop into blastocysts, the potential was related to their donor embryos closely. They seem to follow development pattern of their donor embryos. The blastomere- derived blastocysts have smaller size and fewer cells compared with regular in vitro cultured human embryos. However, AKP expression showed ICM cells with pluripotency. We believe that the value of single blastomere of discarded embryos will be further confirmed in the future.

关键词： discarded embryo single blastomere in-vitro development

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人废弃胚胎单卵裂球体外发育潜能研究

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生殖与避孕 2010年第3期30卷 164-169页

作者：纪冬梅孙贻娟张爱军陆小溦牛志宏冯云上海交通大学医学院附属瑞金医院妇产科生殖医学中心上海200025

目的:探讨体外受精治疗周期废弃胚胎来源的单卵裂球体外发育潜能。方法:收集21例患者授精培养后第3日不适于移植和冷冻的废弃胚胎共60枚,其中2-细胞期胚胎10枚,3-细胞期胚胎8枚,4-细胞期胚胎26枚,5-细胞期胚胎12枚,6-细胞期胚胎4枚。采... 详细信息

目的:探讨体外受精治疗周期废弃胚胎来源的单卵裂球体外发育潜能。方法:收集21例患者授精培养后第3日不适于移植和冷冻的废弃胚胎共60枚,其中2-细胞期胚胎10枚,3-细胞期胚胎8枚,4-细胞期胚胎26枚,5-细胞期胚胎12枚,6-细胞期胚胎4枚。采用化学法去除透明带并分割胚胎获得单卵裂球,继续体外培养,每24h观察卵裂球发育情况。结果:60枚胚胎共获得229枚单卵裂球。以分割取得单卵裂球日作为第1日(d1),继续培养,其发育似乎遵循d2分裂、d3紧密化、d4出现囊胚腔、d5扩张,延续分割前的程序,但提前进入紧密化期,致使囊胚细胞数少,囊胚期体积亦比正常胚胎小。其中,4-细胞期废弃胚胎来源的卵裂球分裂率、紧密化率、囊胚形成率显著高于来自其它细胞数的单卵裂球(P<0.05),且其大部分内细胞团(ICM)细胞的碱性磷酸酶(AKP)表达呈阳性。结论:废弃胚胎来源的单卵裂球能在体外继续发育至囊胚,发育潜能与供体胚胎发育质量密切相关,其发育似乎延续原有供体卵裂球的节律,而形成囊胚所需时间明显缩短,细胞数亦明显减少,但囊胚ICM细胞显示AKP阳性,深信其利用价值会得到进一步证实。

关键词：废弃胚胎单卵裂球发育潜能

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体质量指数对体外受精-胚胎移植结局的影响

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上海交通大学学报（医学版） 2010年第5期30卷 597-600页

作者：关小红张爱军陆小溦孙贻娟牛志宏冯云上海交通大学医学院瑞金医院生殖医学中心上海200025

目的探讨体质量指数(BMI)对体外受精-胚胎移植(IVF-ET)或卵母细胞质内单精子显微注射(ICSI)治疗过程和结局的影响。方法将323例首次应用长方案促排卵行IVF-ET或ICSI的患者,依据BMI分为三组。A组(n=43):BMI24.9kg/m2。比较各组治疗过程... 详细信息

目的探讨体质量指数(BMI)对体外受精-胚胎移植(IVF-ET)或卵母细胞质内单精子显微注射(ICSI)治疗过程和结局的影响。方法将323例首次应用长方案促排卵行IVF-ET或ICSI的患者,依据BMI分为三组。A组(n=43):BMI24.9kg/m2。比较各组治疗过程中各项指标及治疗结果。结果 C组平均超促排卵天数和重组卵泡刺激素(rFSH)用量较A、B两组明显增多(P0.05)。结论 BMI过高导致IVF或ICSI助孕过程中超促排卵天数和rFSH用量增加,hCG注射日E2峰值、大卵泡数和获卵数降低,但对妊娠结局无明显影响。

关键词：体质量指数卵巢反应体外受精-胚胎移植卵胞质内单精子显微注射妊娠率

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胚胎早裂联合胚胎发育速度及形态评分在胚胎选择中的应用

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上海交通大学学报（医学版） 2009年第6期29卷 719-721页

作者：孙贻娟张爱军陆小溦牛志宏陈骞冯云上海交通大学医学院瑞金医院生殖医学中心上海200025

目的探讨胚胎早裂联合胚胎发育速度及形态评分对于体外授精/卵母细胞单精子显微注射(IVF/ICSI)周期中胚胎选择的意义。方法IVF/ICSI 610周期,随机分为A、B两组。A组(269周期)单以授精后72 h(D3)胚胎发育速度及形态评分选择胚胎;B组(341... 详细信息

目的探讨胚胎早裂联合胚胎发育速度及形态评分对于体外授精/卵母细胞单精子显微注射(IVF/ICSI)周期中胚胎选择的意义。方法IVF/ICSI 610周期,随机分为A、B两组。A组(269周期)单以授精后72 h(D3)胚胎发育速度及形态评分选择胚胎;B组(341周期)在此基础上联合胚胎早裂情况进行胚胎选择。比较两组的临床妊娠率和单胚着床率;比较B组移植胚胎中有早裂胚胎和无早裂胚胎者的妊娠结局。结果B组临床妊娠率和单胚着床率均高于A组(P<0.05);B组移植胚胎中有早裂胚胎者的临床妊娠率和单胚着床率均高于无早裂胚胎者(P<0.01)。结论与单纯的胚胎发育速度及形态评分比较,胚胎早裂联合胚胎发育速度及形态评分进行胚胎评价可显著改善IVF/ICSI的临床结局。

关键词：胚胎评价早裂体外受精-胚胎移植卵母细胞单精子显微注射

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Timing analysis of the black hole candidate EXO 1846–031 with Insight-HXMT monitoring

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Research in Astronomy and Astrophysics 2021年第3期21卷 193-204页

作者： He-Xin Liu Yue Huang Guang-Cheng xiao Qing-Cui Bu jin-lu Qu Shu zhang Shuang-Nan zhang Shu-mei jia Fang-jun lu Xiang Ma Lian Tao wei zhang Li Chen Li-Ming Song Ti-Pei Li Yu-Peng Xu Xue-Lei Cao Yong Chen Cong-Zhan Liu Ce Cai zhi Chang Gang Chen Tian-Xiang Chen yi-Bao Chen Yu-Peng Chen wei Cui wei-wei Cui jing-Kang Deng Yong-wei dong Yuan-Yuan Du Min-Xue Fu Guan-Hua Gao He Gao Min Gao Ming-Yu Ge Yu-dong Gu Ju Guan Cheng-Cheng Guo Da-wei Han jia Huo lu-Hua jiang wei-Chun jiang jing jin Yong-jie jin Ling-Da Kong Bing Li Cheng-Kui Li Gang Li Mao-Shun Li wei Li Xian Li xiao-Bo Li Xu-Fang Li Yang-Guo Li Zheng-wei Li xiao-Hua Liang jing-Yuan Liao Bai-Sheng Liu Guo-Qing Liu hong-wei Liu xiao-jing Liu yi-Nong Liu Bo lu Xue-feng lu Qi luo Tao luo Bin Meng yi Nang jian-yin Nie Ge Ou Na Sai Ren-Cheng Shang Xin-ying Song Liang sun ying Tan You-Li Tuo Chen Wang Guo-feng Wang juan Wang Ling-jun Wang Weng-Shuai Wang Yu-Sa Wang Xiang-Yang Wen Bai-Yang Wu Bo-Bing Wu mei Wu Shuo xiao Shao-Lin Xiong He Xu jia-wei Yang Sheng Yang Yan-ji Yang yi-jung Yang Qi-Bin yi Qian-Qing yin Yuan You ai-mei zhang Cheng-Mo zhang Fan zhang hong-mei zhang juan zhang Tong zhang Wan-Chang zhang Wen-Zhao zhang yi zhang yi-Fei zhang Yong-jie zhang Yuan-Hang zhang Yue zhang Zhao zhang zhi zhang Zi-Liang zhang Hai-Sheng Zhao xiao-Fan Zhao Shi-jie Zheng Yao-Guang Zheng Deng-Ke Zhou jian-feng Zhou Yu-Xuan Zhu Ren-Lin Zhuang Yue Zhu Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China Institut fur Astronomie und Astrophysik Kepler Center for Astro and Particle PhysicsEberhard Karls Universität72076 TubingenGermany Department of Astronomy Beijing Normal UniversityBeijing 100088China Department of Astronomy Tsinghua UniversityBeijing 100084China Department of Physics Tsinghua UniversityBeijing 100084China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Space Astronomy and Technology National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China College of Physics Sciences&Technology Hebei UniversityBaoding 071002Hebei ProvinceChina

We present the observational results from a detailed timing analysis of the black hole candidate EXO 1846-031 during its outburst in 2019 with the observations of Insight-HXMT,NICER and *** outburst can be classified roughly into four different ***-C quasi-periodic oscillations(QPOs)observed by NICER(about 0.1-6 Hz)and Insight-HXMT(about 0.7-8 Hz)are also reported in this ***,we study various physical quantities related to QPO *** QPO rms-frequency relationship in the energy band 1-10 keV indicates that there is a turning pointing in frequency around2 Hz,which is similar to that of GRS 1915+105.A possible hypothesis for the relationship above may be related to the inclination of the source,which may require a high inclination to explain *** relationships between QPO frequency and QPO rms,hardness,total fractional rms and count rate have also been found in other transient sources,which can indicate that the origin of type-C QPOs is non-thermal.

关键词： X-ray binaries black hole candidate QPO EXO 1846–031

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Measurement of the ^(232)Th(n,f)cross section in the 1-200 MeV range at the CSNS Back-n

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Nuclear Science and Techniques 2023年第8期34卷 142-153页

作者： zhi-Zhou Ren yi-wei Yang Yong-Hao Chen Rong Liu Bang-jiao Ye jie Wen Hai-Rui Guo Zi-jie Han Qi-Ping Chen Zhong-wei Wen wei-Li sun Han yi Xing-Yan Liu Tao Ye jiang-Bo Bai Qi An jie Bao Yu Bao Ping Cao Hao-Lei Chen Zhen Chen Zeng-Qi Cui Rui-Rui Fan Chang-Qing feng Ke-Qing Gao xiao-Long Gao Min-Hao Gu Chang-Cai Han Guo-Zhu He Yong-Cheng He Yang hong yi-wei Hu Han-Xiong Huang Xi-Ru Huang Hao-Yu jiang wei jiang zhi-jie jiang Han-Tao jing Ling Kang Bo Li Chao Li jia-Wen Li Qiang Li xiao Li Yang Li jie Liu Shu-Bin Liu Ze Long Guang-Yuan luan Chang-jun Ning Meng-Chen niu Bin-Bin Qi jie Ren Xi-Chao Ruan Zhao-Hui Song Kang sun zhi-jia sun zhi-Xin Tan jing-Yu Tang Xin-yi Tang Bin-Bin Tian Li-jiao Wang Peng-Cheng Wang Zhao-Hui Wang xiao-Guang Wu Xuan Wu Li-Kun Xie xiao-yun Yang Li Yu Tao Yu Yong-ji Yu Guo-Hui zhang Lin-Hao zhang Qi-wei zhang Xian-Peng zhang Yu-Liang zhang zhi-Yong zhang lu-Ping Zhou zhi-Hao Zhou Ke-jun Zhu Department of Modern Physics University of Science and Technology of ChinaHefei 230026China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang 621900China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China Institute of Applied Physics and Computational Mathematics Beijing 100088China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230027China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijingChina Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China Department of Engineering and Applied Physics University of Science and Technology of ChinaHefei 230026China

The ^(232)Th(n,f)cross section is very important in basic nuclear physics and applications based on the Th/U fuel *** the time-of-flight method and a multi-cell fast-fission ionization chamber,a novel measurement of the^(232)Th(n,f)cross sec-tion relative to^(235)U in the 1–200 MeV range was performed at the China Spallation Neutron Source Back-n white neutron source(Back-n).The fission event-neutron energy spectra of^(232)Th and^(235)U fission cells were measured in the single-bunch *** 232Th/235U fission cross-sectional ratios were obtained,and the measurement uncertainties were 2.5–3.7%for energies in the 2–20 MeV range and 3.6–6.2%for energies in the 20–200 MeV ***^(232)Th(n,f)cross section was obtained by introducing the standard cross section of^(235)U(n,f).The results were compared with those of previous theoreti-cal calculations,measurements,and *** measured 232Th fission cross section agreed with the main evaluation results in terms of the experimental uncertainty,and 232Th fission resonances were observed in the 1–3 MeV *** present results provide^(232)Th(n,f)cross-sectional data for the evaluation and design of Th/U cycle nuclear systems.

关键词： 232Th(n f)cross section Fast-fission ionization chamber Back-n white neutron source

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冻融胚胎移植周期中胚胎因素的分析研究

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生殖与避孕 2007年第9期27卷 577-580页

作者：孙贻娟冯云张爱军牛志宏陆小溦上海交通大学医学院附属瑞金医院妇产科生殖医学中心上海200025

目的:分析冻融胚胎移植周期中胚胎卵裂球损伤程度与有丝分裂的恢复对于临床结局的影响。方法:171例患者共进行冻融胚胎移植206个周期,采用慢速冷冻法冻存d3胚胎,快速解冻法复苏后继续培养4h后移植,根据复苏后卵裂球的损伤程度分为完整... 详细信息

目的:分析冻融胚胎移植周期中胚胎卵裂球损伤程度与有丝分裂的恢复对于临床结局的影响。方法:171例患者共进行冻融胚胎移植206个周期,采用慢速冷冻法冻存d3胚胎,快速解冻法复苏后继续培养4h后移植,根据复苏后卵裂球的损伤程度分为完整组、混合组和损伤组,另根据复苏后4h胚胎进行分裂的情况分为分裂组,混合组和未分裂组,比较各组间的临床妊娠率和单胚着床率。结果:206个周期共复苏胚胎632枚,复苏存活率85.44%(540/632),移植胚胎508个,周期妊娠率36.41%(75/206),单胚着床率19.88%(101/508),完整组与损伤组间、分裂组与未分裂组间的临床妊娠率和单胚着床率都有显著性差异(P<0.05)。结论:冻融胚胎移植周期中胚胎卵裂球的损伤程度与有丝分裂的恢复是影响临床结局的重要因素。

关键词：冻融胚胎胚胎分裂

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Back-n white neutron source at CSNS and its applications

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Nuclear Science and Techniques 2021年第1期32卷 95-104页

作者： jing-Yu Tang Qi An jiang-Bo Bai jie Bao Yu Bao Ping Cao Hao-Lei Chen Qi-Ping Chen Yong-Hao Chen Zhen Chen Zeng-Qi Cui Rui-Rui Fan Chang-Qing feng Ke-Qing Gao xiao-Long Gao Min-Hao Gu Chang-Cai Han Zi-jie Han Guo-Zhu He Yong-Cheng He Yang hong yi-wei Hu Han-Xiong Huang Xi-Ru Huang Hao-Yu jiang wei jiang zhi-jie jiang Han-Tao jing Ling Kang Bo Li Chao Li jia-Wen Li Qiang Li xiao Li Yang Li jie Liu Rong Liu Shu-Bin Liu Xing-Yan Liu Ze Long Guang-Yuan luan Chang-jun Ning Meng-Chen niu Bin-Bin Qi jie Ren zhi-Zhou Ren Xi-Chao Ruan Zhao-Hui Song Kang sun zhi-jia sun zhi-Xin Tan Xin-yi Tang Bin-Bin Tian Li-jiao Wang Peng-Cheng Wang Zhao-Hui Wang Zhong-wei Wen xiao-Guang Wu Xuan Wu Li-Kun Xie xiao-yun Yang yi-wei Yang Han yi Li Yu Tao Yu Yong-ji Yu Guo-Hui zhang Lin-Hao zhang Qi-wei zhang Xian-Peng zhang Yu-Liang zhang zhi-Yong zhang lu-Ping Zhou zhi-Hao Zhou Ke-jun Zhu 无 State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang 621900China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Spallation Neutron Source Science Center Dongguan 523803China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China Department of Engineering and Applied Physics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China The CSNS Back-n Collaboration

Back-streaming neutrons from the spallation target of the China Spallation Neutron Source(CSNS)that emit through the incoming proton channel were exploited to build a white neutron beam facility(the so-called Back-n white neutron source),which was completed in March *** Back-n neutron beam is very intense,at approximately 29107 n/cm2/s at 55 m from the target,and has a nominal proton beam with a power of 100 kW in the CSNS-I phase and a kinetic energy of 1.6 GeV and a thick tungsten target in multiple slices with modest moderation from the cooling water through the *** addition,the excellent energy spectrum spanning from 0.5 eV to 200 MeV,and a good time resolution related tothe time-of-flight measurements make it a typical white neutron source for nuclear data measurements;its overall performance is among that of the best white neutron sources in the *** with advanced spectrometers,detectors,and application utilities,the Back-n facility can serve wide applications,with a focus on neutron-induced cross-sectional *** article presents an overview of the neutron beam characteristics,the experimental setups,and the ongoing applications at Backn.

关键词： White neutron source Nuclear data measurements Experimental setups Neutron applications

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体外助孕治疗周期中分半进行IVF和ICSI的指征

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上海交通大学学报（医学版） 2008年第3期28卷 256-259页

作者：陆小溦冯云张爱军孙贻娟张慧琴牛志宏上海交通大学医学院瑞金医院妇产科生殖医学中心上海200025

目的对体外助孕治疗周期中存在不受精可能且上游有效精子数量不低于500万的夫妇分半进行常规体外受精（IVF）与卵胞浆内单精子显微注射（ICSI）治疗，探讨是否存在分半治疗的必要性以及指征选择的可行性。方法2002年3月～2007年6月接受... 详细信息

目的对体外助孕治疗周期中存在不受精可能且上游有效精子数量不低于500万的夫妇分半进行常规体外受精（IVF）与卵胞浆内单精子显微注射（ICSI）治疗，探讨是否存在分半治疗的必要性以及指征选择的可行性。方法2002年3月～2007年6月接受体外助孕的夫妇中选取符合指征周期，采用常规超排卵方案，同一周期取得的卵子随机分半，分别进行常规IVF与ICSI治疗。卵裂球≥5个且卵裂球碎片〈50％的胚胎为可用胚胎。结果共有94周期进入分半治疗周期，其中常规IVF受精失败或低下（LFR）22周期（23．40％）；同期常规IVF治疗530周期，其中LFR36周期（6．79％）；两者有显著差异（P=5．26×10^-6）。94分半周期共获1596枚卵子，其中行常规IVF781枚，受精率55．19％；行ICSI815枚，MⅡ616枚，受精率72．02％；两者有显著差异（P=2．59×10^-12）。94周期卵裂983枚，可用胚胎554枚，临床妊娠率为35．7l％。结论存在同一治疗周期分半进行常规IVF与ICSI的指征和必要性，可明显减少不受精情况的出现；对常规IVF与ICSI均受精的周期，可选择移植常规IVF胚胎，减少子代遗传和先天缺陷风险。

关键词：分半周期体外受精卵胞浆内单精子显微注射受精失败或低下发生率

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不同胚胎选择体系对IVF-ET妊娠结局的影响

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生殖与避孕 2009年第1期29卷 26-30页

作者：孙贻娟张爱军冯云张慧琴牛志宏陆小溦上海交通大学医学院附属瑞金医院妇产科生殖医学中心上海200025 上海市计划生育科学研究所上海200032 复旦大学上海200032

目的:探讨IVF-ET周期中不同胚胎选择体系对妊娠结局的影响。方法:将530个IVF-ET周期随机分为3组,按不同的胚胎选择方法选择胚胎进行移植。A组:228个移植周期,根据d3胚胎常规评分方法选择胚胎;B组:140个移植周期,根据d3胚胎常规评分结合... 详细信息

目的:探讨IVF-ET周期中不同胚胎选择体系对妊娠结局的影响。方法:将530个IVF-ET周期随机分为3组,按不同的胚胎选择方法选择胚胎进行移植。A组:228个移植周期,根据d3胚胎常规评分方法选择胚胎;B组:140个移植周期,根据d3胚胎常规评分结合原核评分选择胚胎;C组:162个移植周期,根据d3胚胎常规评分结合胚胎早期分裂选择胚胎。将C组进一步分为移植胚胎中有早裂胚胎和无早裂胚胎二组。比较各组的临床妊娠率和单胚着床率。结果:B组的临床妊娠率和单胚种植率为37.86%和21.77%,与A组(33.33%和20.56%)比均无统计学差异(P>0.05);而C组(45.06%和30.88%)均显著高于A组(P<0.05);C组移植胚胎中有早裂胚胎者的临床妊娠率和单胚着床率为55.68%和38.38%,分别显著高于移植胚胎中不含早裂胚胎者(32.43%和21.94%)(P<0.05)。结论:d3胚胎常规评分结合胚胎早期分裂选择的胚胎移植,可以提高IVF-ET周期的临床妊娠率和单胚着床率。

关键词：胚胎选择原核评级早期卵裂临床妊娠率单胚着床率

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