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Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts

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Journal of Animal Science and Biotechnology 2021年第4期12卷 1662-1674页

作者： Jinsol Jeong Kwang-Hwan Choi Seung-Hun Kim dong-kyung lee Jong-Nam Oh Mingyun lee Gyung Cheol Choe Chang-Kyu lee Department of Agricultural Biotechnology Animal Biotechnology Majorand Research Institute of Agriculture and Life ScienceSeoul National UniversitySeoul 08826South Korea Present address:Research and Development Center Space F corporationHwasung-siGyeonggi-do 18471South Korea Institute of Green Bio Science and Technology Seoul National UniversityPyeong ChangKangwon-do 25354South Korea

Background:Myogenic transdifferentiation can be accomplished through ectopic MYOD1 expression,which is facilitated by various signaling pathways associated with *** this study,we attempted to transdifferentiate pig embryonic fibroblasts(PEFs)myogenically into skeletal muscle through overexpression of the pig MYOD1 gene and modulation of the FGF,TGF-β,WNT,and cAMP signaling ***:The MYOD1 overexpression vector was constructed based on comparative sequence analysis,demonstrating that pig MYOD1 has evolutionarily conserved domains across various *** forced MYOD1 expression through these vectors triggered the expression of endogenous muscle markers,transdifferentiated muscle cells from fibroblasts were not ***,various signaling molecules,including FGF2,SB431542,CHIR99021,and forskolin,along with MYOD1 overexpression were applied to enhance the myogenic *** modified conditions led to the derivation of myotubes and activation of muscle markers in PEFs,as determined by qPCR and ***,a sarcomere-like structure was observed,indicating that terminally differentiated skeletal muscle could be obtained from transdifferentiated ***:In summary,we established a protocol for reprogramming MYOD1-overexpressing PEFs into the mature skeletal muscle using signaling *** myogenic reprogramming can be used as a cell source for muscle disease models in regenerative medicine and the production of cultured meat in cellular agriculture.

关键词： MYOD1 Pig Sequence analysis Skeletal muscle Transdifferentiation

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Aligned Ion Conduction Pathway of Polyrotaxane‑Based Electrolyte with Dispersed Hydrophobic Chains for Solid‑State Lithium–Oxygen Batteries

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Nano-Micro Letters 2025年第2期17卷 169-186页

作者： Bitgaram Kim Myeong‑Chang Sung Gwang‑Hee lee Byoungjoon Hwang Sojung Seo Ji‑Hun Seo dong‑Wan Kim Department of Materials Science and Engineering Korea University145 Anam‑RoSeongbuk‑GuSeoul 02841Republic of Korea School of Civil Environmental and Architectural EngineeringKorea University145 Anam‑RoSeongbuk‑GuSeoul 02841Republic of Korea Materials Science and Chemical Engineering Center Institute for Advanced Engineering(IAE)Yongin 17180Republic of Korea

A critical challenge hindering the practical application of lithium–oxygen batteries(LOBs)is the inevitable problems associated with liquid electrolytes,such as evaporation and safety *** study addresses these problems by proposing a modified polyrotaxane(mPR)-based solid polymer electrolyte(SPE)design that simultaneously mitigates solvent-related problems and improves ***-SPE exhibits high ion conductivity(2.8×10^(−3)S cm^(−1)at 25℃)through aligned ion conduction pathways and provides electrode protection ability through hydrophobic chain *** this mPR-SPE into solid-state LOBs resulted in stable potentials over 300 *** situ Raman spectroscopy reveals the presence of an LiO_(2)intermediate alongside Li_(2)O_(2)during oxygen *** situ X-ray diffraction confirm the ability of the SPE to hinder the permeation of oxygen and moisture,as demonstrated by the air permeability *** present study suggests that maintaining a low residual solvent while achieving high ionic conductivity is crucial for restricting the sub-reactions of solid-state LOBs.

关键词： Solid polymer electrolyte Lithium-oxygen batteries Polyrotaxane ion conductivity Hydrophobic chain

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K2Ti6O13 Nanoparticle-Loaded Porous rGO Crumples for Supercapacitors

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Nano-Micro Letters 2020年第1期12卷 115-123页

作者： Chongmin lee Sun kyung Kim Hankwon Chang Hee dong Jang Department of Nanomaterials Science and Engineering University of Science and TechnologyYuseong‑guDaejeon 34113Republic of Korea Resources Utilization Research Center Korea Institute of Geoscience and Mineral ResourcesYuseong‑guDaejeon 34132Republic of Korea

One-dimensional alkali metal titanates containing potassium,sodium,and lithium are of great concern owing to their high ion mobility and high specific surface *** those titanates are combined with conductive materials such as graphene,carbon nanotube,and carbon nanofiber,they are able to be employed as efficient electrode materials for *** hexa-titanate(K2Ti6O13,KTO),in particular,has shown superior electrochemical properties compared to other alkali metal titanates because of their large lattice parameters induced by the large radius of potassium ***,we present porous rGO crumples(PGC)decorated with KTO nanoparticles(NPs)for application to *** KTO NP/PGC composites were synthesized by aerosol spray pyrolysis and post-heat *** NPs less than 10 nm in diameter were loaded onto PGCs ranging from 3 to 5μ*** porous structure of the composites was obtained by the activation of rGO by adding an excessive amount of KOH to the *** KTO NP/PGC composite electrodes fabricated at the GO/KOH/TiO2 ratio of 1:3:0.25 showed the highest performance(275 F g−1)in capacitance with different KOH concentrations and cycling stability(83%)after 2000 cycles at a current density of 1 A g−1.

关键词： Potassium hexa-titanate Porous graphene crumples Supercapacitors

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Effect of Fe_2P in LiFePO_4/Fe_2P composite on electrochemical properties synthesized by MA and control of heat condition

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Rare Metals 2006年第z2期25卷 179-183页

作者： PARK Jong Suk lee kyung Tae lee kyung Sub Department of Material Science and Engineering Hanyang University Seoul 133-731 Korea

In order to control the size and distribution of the high conductive Fe2P in LiFePO4/Fe2P composite, two different cooling rates (Fast: 15 ℃·min-1, Slow: 2 ℃·min-1) were employed after mechanical alloying. The discharge capacity of the fast cooled was 83 mAh·g-1 and the slow cooled 121 mAh·g-1. The particle size of the synthesized powder was examined by transmission electron microscopy and distribution of Fe2P was characterized using scanning electron microscopy (SEM). In addition, two-step heat treatment was carried out for better distribution of Fe2P. X-ray diffraction (XRD) and Rietveld refinement reveal that LiFePO4/Fe2P composite consists of 95.77% LiFePO4 and 4.33% of Fe2P.

关键词： LiFePO4/Fe2P composite mechanical alloying heat treatment discharge capacity

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Intermittent, low-dose, antiandrogen monotherapy as an alternative therapeutic option for patients with positive surgical margins after radical prostatectomy

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Asian Journal of Andrology 2018年第3期20卷 270-275页

作者： kyung Hwa Choi Seung Ryeol lee Young Kwon Hong dong Soo Park Department Urology CHA Bundang Medical Center CHA University Seongnam Korea.

The aim of the present study was to determine whether oncologic outcomes and adverse events associated with active on/off intermittent antiandrogen monotherapy （daily bicalutamide, 50 mg per day） are comparable with those of standard external beam radiation therapy （EBRT） or combined androgen blockade （CAB） therapy in prostate cancers with positive surgical margins after radical prostatectomy. Two hundred twenty-three patients with positive surgical margins post-radical prostatectomy who underwent active surveillance （AS, n = 32）, EBRT without hormone therapy （n = 55）, intermittent antiandrogen monotherapy without EBRT （IAAM, n= 50）, or CAB without EBRT （n = 86）, between 2007 and 2014, were reviewed retrospectively. Pathologic outcomes, biochemical recurrence rates, radiological disease progression, and adverse events were collected from medical records. Biochemical recurrence rates, biochemical recurrence-free survival rates, and radiological recurrence were not different between the groups （P = 0.225, 0.896, and 0.284, respectively）. Adverse event rates and severities were lower for IAAM compared with EBRT or CAB （both P 〈 0.05）, but were comparable to those for AS （P = 0.591 and 0.990, respectively）. Grade ：〉3 adverse events were not reported in the IAAM or AS groups. Erectile dysfunction and loss of libido rates were lower in the IAAM group compared with the EBRT and CAB groups （P = 0.032）. Gastrointestinal complications were more frequently reported in the EBRT group （P = 0.008）. Active on/off IAAM treatment might be an appropriate treatment option for patients with positive surgical margins after radical prostatectomy. Furthermore, regarding oncologic outcomes, IAAM was comparable to standard EBRT but had a milder adverse event profile.

关键词： androgen receptor antagonists disease progression margins of excision prostatectomy prostatic neoplasms

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Selective Solid-phase Extraction of Aloe Emodin from Aloe by Molecularly Imprinted Polymers

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Chemical Research in Chinese Universities 2013年第4期29卷 663-666页

作者： TIAN Ming-lei lee Yu-ri PARK dong-wha ROW kyung-ho College of Chemistry and Environmental Engineering Yangtze University Jingzhou 434023 P R. China Department of Chemical Engineering Regional Innovation Center for Environmental Technology of Thermal Plasma Inha University Ineheon 402-751 Korea

The extraction and separation of aloe emodin were optimized via selective molecularly imprinted solid-phase extraction. Molecularly imprinted polymer was prepared from the functional monomer, methacrylic acid and a mixture of ethanol/dodecanol（90/10, volume ratio） as porogen. It overcomes the common problems of imprin- ting biological polar compounds and shows high selectivity compared favorably with those of non-imprinted polymer and commercially available C18 and silica cartridges in similar aloe emodin tests. Good linearity was obtained be- tween 0.002 and 2.5 mg/mL（r2=0.998） with relative standard deviations below 3.3%.

关键词： Aloe emodin Molecularly imprinted polymer Solid-phase extraction High performance liquid chromato- graphy

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PGLYRP1 protein as a novel mediator of cellular dialogue in neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 1993-1994页

作者： Anup Bhusal Won-Ha lee Kyoungho Suk Department of Pharmacology School of MedicineKyungpook National UniversityDaeguRepublic of Korea BK21 Plus KNU Biomedical Convergence Program Department of Biomedical ScienceSchool of MedicineKyungpook National UniversityDaeguRepublic of Korea School of Life Sciences BK21 plus KNU Creative BioResearch GroupKyungpook National UniversityDaeguRepublic of Korea Brain Science and Engineering Institute Kyungpook National UniversityDaeguRepublic of Korea

Neuroinflammation has been identified as a crucial element in several neurological disorders. Glial cells play a critical role in directing neuroinflammation, both in deleterious and beneficial ways.

关键词： inflammation considerably

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Single-cell and spatial omics:exploring hypothalamic heterogeneity

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Neural Regeneration Research 2025年第6期20卷 1525-1540页

作者： Muhammad Junaid Eun Jeong lee Su Bin Lim Department of Biochemistry&Molecular Biology Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Graduate School of Ajou UniversitySuwonSouth Korea Department of Brain Science Ajou University School of MedicineSuwonSouth Korea

Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions. Over the past decade, single-cell and spatial omics technologies have significantly evolved, overcoming initial technical challenges in capturing and analyzing individual cells. These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus. Current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus. However, single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue, potentially resulting in the loss of spatial information concerning neuronal networks. Spatial transcriptomics methods, by bypassing the cell dissociation, can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies. In this review, we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types, driven by recent high-throughput achievements.

关键词： cellular diversity hypothalamus multi-omics single-cell transcriptomics spatial transcriptomics

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Effect of Nucleases on the Cellular Internalization of Fluorescent Labeled DNA-Functionalized Single-Walled Carbon Nanotubes

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Nano Research 2008年第4期1卷 351-360页

作者： Hye kyung Moon Chan Il Chang dong-Ki lee Hee Cheul Choi Department of Chemistry Pohang University of Science and Technology(POSTECH)San 31Hyoja-DongNam-guPohang 790-784Korea Department of Chemistry Sungkyunkwan UniversityNatural Science CampusSuwon 440-746Korea

Nuclease effects on the cell internalization of single-walled carbon nanotubes(SWNTs)functionalized with fluorescent-labeled DNA in serum containing cell growth media were *** Cy3-labeled DNA-functionalized SWNT conjugates(Cy3DNA-SWNTs)were incubated with HeLa cells in a fatal bovine serum(FBS)medium,a high fl uorescence intensity was obtained from the cells,indicative for the high level inclusion of ***,the fluorescence intensity was remarkably reduced if Cy3DNA-SWNTs were incubated with cells in the FBS-free *** systematic control experiments revealed that Cy3 dye molecules were released from Cy3DNA-SWNT conjugates by nuclease,and the free Cy3 dyes penetrate into HeLa cell with high *** the actual amounts of SWNTs internalized in the cells were almost identical for both cells incubated in the FBS-present and FBS-absent media according to the Raman measurements,one should be cautious to determine the degree of SWNT internalization based on the fl uorescence intensities especially when the coloring dye molecules were linked to oligonucleotides in nuclease containing media.

关键词： Single-walled carbon nanotube oligonucleotide cellular delivery molecular transporter nuclease

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Determination of diffusion coefficient by image-based fluorescence recovery after photobleaching and single particle tracking system implemented in a single platform

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Journal of Innovative Optical Health Sciences 2021年第2期4卷 93-101页

作者： donghee lee Jeonghoon lee Jung kyung Kim Department of Genetics Cell Biology and Anatomy University of Nebraska Medical CenterOmahaNE 68198USA School of Mechanical Engineering KOREA TECH Cheonan 31253Republic of Korea School of Mechanical Engineering and Department of Integrative Biomedical Science and Engineering Graduate School Kookmin University Seoul 02707Republic of Korea

Fluorescence recovery after photobleaching(FRAP)and single particle tracking(SPT)techni-ques determine the diffusion coefficient from average diffusive motion of high-concentration molecules and from trajectories of low-concentration single molecules,*** dif-fusion coefficients measured by FRAP and SPT techniques for the same biomolecule on cell membrane have exhibited inconsistent values across laboratories and platforms with larger dif-fusion coefficient determined by FRAP,but the sources of the inconsistency have not been investigated ***,we designed an image-based FRAP-SPT system and made a direct comparison between FRAP and SPT for diffusion coefficient of submicron particles with known theoretical values derived from Stokes-Einstein equation in aqueous *** combined iFRAP-SPT technique allowed us to measure the diffusion coefficient of the same fluorescent particle by utilizing both techniques in a single platform and to scrutinize inherent errors and artifacts of *** results reveal that diffusion coefficient overestimated by FRAP is caused by inaccurate estimation of the bleaching spot size and can be corrected by simple image *** iFRAP-SPT technique can be potentially used for not only cellular membrane dynamics but also for quantitative analysis of the spatiotemporal distribution of the solutes in small scale analytical devices.

关键词： Diffusion coefficient fluorescence recovery after photobleaching(FRAP) single particle tracking(SPT)

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