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Association between FOXO3A gene polymorphisms and human longevity： a meta-analysis

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Asian Journal of Andrology 2014年第3期16卷 446-452页

作者： Ji-Ming Bao Xian-Lu Song Ying-Qia Hong hai-Li zhu Cui Li Tao Zhang Wei Chen Shan-Chao Zhao Qing Chen Department of Urology and Medical Center for Overseas Patients Nanfang HospitaIGuangzhou Department of Radiotherapy Cancer Center of Guangzhou Medical University Guangzhou Department of Pediatric Surgery The First Affiliated Hospital of Shantou University Medical College Shantou School of Basic Medical Sciences Southern Medical University Guangzhou Department of Epidemiology School of Public Health and Tropical Medicine Southern Medical University Guangzhou China.

Numerous studies have shown associations between the FOXO3A gene, encoding the forkhead box 03 transcription factor, and human or specifically male longevity. However, the associations of specific FOXO3A polymorphisms with longevity remain inconclusive. We performed a meta-analysis of existing studies to clarify these potential associations. A comprehensive search was conducted to identify studies of FOXO3A gene polymorphisms and longevity. Pooled odds ratios （ORs） and 95% confidence intervals （Cls） were calculated by comparing the minor and major alleles. A total of seven articles reporting associations of FOXO3A polymorphisms with longevity were identified and included in this meta-analysis. These comprised 11 independent studies with 5241 cases and 5724 controls from different ethnic groups, rs2802292, rs2764264, rs13217795, rs1935949 and rs2802288 polymorphisms were associated with human longevity （OR = 1.36, 95% Cl = 1.10-1.69, P= 0.005; OR = 1.20, 95% CI = 1.04-1.37, P= 0.01; OR = 1.27, 95% CI = 1.10-1.46, P= 0.001; OR = 1.14, 95% CI = 1.01-1.27 and OR = 1.24, 95% CI = 1.07-1.43, P= 0.003, respectively）. Analysis stratified by gender indicated significant associations between rs2802292, rs2764264 and rs13217795 and male longevity （OR = 1.54, 95% Cl = 1.33-1.79, P〈 0.001; OR = 1.38, 95% Cl = 1,15-1.66, P= 0.001; and OR = 1.39, 95% Cl = 1.15-1.67, P= 0.001）, but rs2802292, rs2764264 and rs1935949 were not linked to female longevity. Moreover, our study showed no association between rs2153960, rs7762395 or rs13220810 polymorphisms and longevity. In conclusion, this meta-analysis indicates a significant association of five FOXO3A gene polymorphisms with longevity, with the effects of rs2802292 and rs2764264 being male-specific, Further investigations are required to confirm these findings.

关键词： FOXO3A longevity meta-analysis polymorphism

核酸扩增技术及其在动物检疫中的应用、挑战与对策

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中国口岸科学技术 2024年第2期6卷 32-39页

作者：田纯见刘建利梅明珠王莹丁宁鱼海琼李群辉广州海关技术中心广州510623 深圳海关动植物检验检疫技术中心深圳518045 广东省温氏集团研究院云浮527300

核酸扩增技术是一种在生物技术领域广泛使用的技术,可通过特定的方法将DNA或RNA片段扩增至百万倍甚至更高倍数,从而检测和识别这些片段。核酸扩增技术包括多种方法,如实时荧光定量PCR(quantitative real-time PCR,qPCR)、数字PCR技术(di... 详细信息

核酸扩增技术是一种在生物技术领域广泛使用的技术,可通过特定的方法将DNA或RNA片段扩增至百万倍甚至更高倍数,从而检测和识别这些片段。核酸扩增技术包括多种方法,如实时荧光定量PCR(quantitative real-time PCR,qPCR)、数字PCR技术(digital PCR,dPCR)、重组酶聚合酶扩增技术(recombinase polymerase amplification,RPA)等。在动物检疫方面,通过对动物体内特定病原体的核酸进行扩增,可以快速、准确地检测出该病原体,并采取相应的防控措施;通过对动物源性食品中的病原微生物进行核酸扩增,可以检测出食品中的细菌、病毒等有害物质,保证食品的安全性。随着技术的发展,核酸扩增技术将更加自动化、高通量和快速,灵敏度和特异性将进一步提高。随着微流控技术和纳米技术的发展,核酸扩增技术将变得更加便携,与人工智能、大数据等技术结合,实现智能化和信息化检测,多学科交叉将成为核酸扩增技术发展的重要趋势,有助于推动该技术的创新和应用。

关键词：实时荧光定量PCR 数字PCR 环介导等温扩增重组酶聚合酶扩增动物检疫

Differences in phenotype and gene expression of prostate stromal cells from patients of varying ages and their influence on tumour formation by prostate epithelial cells

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Asian Journal of Andrology 2011年第5期13卷 732-741页

作者： Yong-Chuan Wang Sheng-Qiang Yu Xiao-hai Wang Bang-Min Han Fu-Jun Zhao Guang-Hui zhu Yan Hong Shu-Jie Xia Department of Urology The Affiliated First People＇s Hospital of Shanghai Jiao Tong University School of Medicine Shanghai 200080 China Department of Urology Yantai Yuhuangding Hospital Affiliated with the Medical College of Qingdao University Shandong 264000 China Department of General Surgery The Affiliated First People＇s Hospital of Shanghai Jiao Tong University Shanghai 200080 China

Prostate cancer （PCa） is an age-related disease, and the stromal microenvironment plays an important role in prostatic malignant progression. However, the differences in prostate stromal cells present in young and old tissue are still obscure. We established primary cultured stromal cells from normal prostatic peripheral zone （PZ） of donors of varying ages and found that cultured stromal cells from old donors （PZ-old） were more enlarged and polygonal than those from young donors （PZ-young）. Furthermore, based on immunocytochemical and ultrastructural analysis, the components of stromal cells changed from a majority of fibroblasts to a mixture of fibroblasts and myofibroblasts with increasing donor age. Using a three-dimensional in vitro culture system, we found that PZ-old stromal cells could enhance the proliferation, migration and invasion of cocultured benign BPH-1 and PC-3 cells. Using an in vivo tissue recombination system, we also found that PZ-old stromal cells are more effective than PZ-young cells in promoting tumour formation by BPH-1 cells of high passage（〉100） and PC-3 cells. To probe the possible mechanism of these effects, we performed cDNA microarray analysis and profiled 509 upregulated genes and 188 downregulated genes in PZ-old cells. Among the changed genes, we found genes coding for a subset of paracrine factors that are capable of influencing adjacent epithelial cells; these include hepatocyte growth factor （HGF）, fibroblast growth factor 5 （FGF5）, insulin-like growth factor 2 （IGF2）, insulin-like growth factor-binding protein 4 （IGFBP4）, IGFBP5 and matrix metallopeptidase 1 （MMP1）. Changes in the expression of these genes were further confirmed by quantitative real-time polymerase chain reaction （PCR）, Western blotting and enzyme-linked immunosorbent assays. Overall, our findings indicate that stromal cells from prostate PZ of old donors are more active than similar cells from young donors in promoting the malignant process of adjacent epitheli

关键词： coculture gene expression peripheral zone phenotype prostate cancer stromal cells

Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

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Journal of Integrative Agriculture 2013年第1期12卷 103-109页

作者： NIU Bu-yue LAN Xiao-ming YAN Xiao-hong DI Sheng-wei WANG Yang LI hai-tao XIONG Yuan- zhu WANG Xi-biao College of Animal Science and Technology Northeast Agricultural University Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction Ministry of Education/Huazhong Agricultural University

The matrix metalloproteinase 23 （MMP-23）, which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 （pMMP-23） gene, including exons 2-8 （GenBank： EU360790）, was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 （EU360790： g. 269C〉T） and an A/G mutation in exon 4 （EU360790： g. 505A〉G）, respectively. Association study for these two SNPs with litter size was assessed in three independent populations （Minzhu, Landrace I and Landrace II）. Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed （P〈0.05） at g. 269C〉T locus, and the additive effect was significant （P〈0.05）; GG sows produced more TNB and NBA than AA pigs in Minzhu （P〈0.01） and Landrace breeds （P〈0.05） at g. 505A〉G locus, and the additive effect was significant （P〈0.01 or P〈0.05）. Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A〉G locus might be a useful molecular marker for marker assisted selection （MAS）.

关键词： pig MMP-23 molecular cloning expression litter size

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跨江大桥智能钢筋加工厂设备的应用研究

起重运输机械 2024年第11期 83-88页

作者：刘斌胡功学程茂林朱明清董奇峰中交第二航务工程局有限公司武汉430040 中交二航局第四工程有限公司芜湖241000

针对张靖皋长江大桥南航道桥南锚碇钢筋加工厂生产需要,对钢筋加工设备和起重机进行智能化升级。文中通过物流转运装置和光电控制系统对传统钢筋加工设备进行自动化升级改造,集成自动上料机和自动剪切线,基于钢筋云平台与上位机控制系... 详细信息

针对张靖皋长江大桥南航道桥南锚碇钢筋加工厂生产需要,对钢筋加工设备和起重机进行智能化升级。文中通过物流转运装置和光电控制系统对传统钢筋加工设备进行自动化升级改造,集成自动上料机和自动剪切线,基于钢筋云平台与上位机控制系统串联各生产线顺序功能,实现了钢筋从上料、切断到加工的连续自动化生产。通过设置位置传感器和多点工位呼叫台对起重机进行智能化升级,采用智能算法对起重机的避碰和调度功能进行优化,实现了起重机的自动化调度,提高了起重机运行的效率。最后,通过现场应用验证了设备升级方案的可行性,既降低了工程成本,又提高了生产效率。

关键词：钢筋加工厂智能化物流转运装置光电控制系统上料机

SIN3B promotes integrin αV subunit gene transcription and cell migration of hepatocellular carcinoma

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Journal of Molecular Cell Biology 2019年第5期11卷 421-432页

作者： Qianqian Cai Yuanyuan Liu Ping zhu Chunlang Kang Heyang Xu Bing Qi Rong Wang Yiwei dong Xing Zhong Wu Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Fudan University. Key Lab of Glycoconjugate Research Ministry of Public Health Shanghai 200032 China Zhejiang Provincial People's Hospital Hangzhou 310053 China

Paired amphipathic helix protein (SIN3B) is a transcription corepressor for many genes. Here we show a different regulation mechanism of integrin aV gene expression by SIN3B in human hepatocellular carcinoma (HCC). We first observed a close relationship between Integrin aV and SIN3B expressions in HCC patients and tumor cell lines with different metastatic potentials. Overexpression of SIN3B significantly accelerated the cell migration rate of SMMC-7721, but failed when integrin αV expression was silenced. Interestingly, SIN3B stimulated integrin aV subunit promoter activity only in the presence of sulfatide. Importantly, SIN3B was identified in the complex with sulfatide by mass spectrometry. Fat blot assay indicated that SIN3B specifically interacted with sulfatide. Molecular modeling suggested that sulfatide induced the conformational change of SIN3B from compacted α-helices to a relaxed β-sheet in PAH2 domain. The data of immunoprecipitation and ChIP assay indicated that altered SIN3B lost the binding affinity with MAD1 and HDAC2, which reduced the recruitment of HDAC2 on integrin aV gene promoter and prevented the deacetylation of the histone 3. In conclusion, this study demonstrated that SIN3B promoted the transcriptional activation of the integrin αV subunit gene promoter by reducing interaction with HDAC2.

关键词： paired amphipathic helix protein ITGAV sulfatide HCC histone deacetylation

Massive Files Prefetching Model Based on LSTM Neural Network with Cache Transaction Strategy

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Computers, Materials & Continua 2020年第5期63卷 979-993页

作者： dongjie zhu haiwen Du Yundong Sun Xiaofang Li Rongning Qu Hao Hu Shuangshuang dong Helen Min Zhou Ning Cao School of Computer Science and Technology Harbin Institute of TechnologyWeihai264209China School of Science Harbin Institute of TechnologyWeihai264209China School of Engineering Manukau Institute of TechnologyAuckland2241New Zealand College of Mathematics and Computer Science Xinyu UniversityXinyu338004China College of Information Engineering Sanming UniversitySanming365004China School of Astronautics Harbin Institute of TechnologyHarbin150001China

In distributed storage systems,file access efficiency has an important impact on the real-time nature of information *** a popular approach to improve file accessing efficiency,prefetching model can fetches data before it is needed according to the file access pattern,which can reduce the I/O waiting time and increase the system ***,prefetching model needs to mine the degree of association between files to ensure the accuracy of *** the massive small file situation,the sheer volume of files poses a challenge to the efficiency and accuracy of relevance *** this paper,we propose a massive files prefetching model based on LSTM neural network with cache transaction strategy to improve file access ***,we propose a file clustering algorithm based on temporal locality and spatial locality to reduce the computational ***,we propose a definition of cache transaction according to files occurrence in cache instead of time-offset distance based methods to extract file block feature ***,we innovatively propose a file access prediction algorithm based on LSTM neural network which predict the file that have high possibility to be *** show that compared with the traditional LRU and the plain grouping methods,the proposed model notably increase the cache hit rate and effectively reduces the I/O wait time.

关键词： Massive files prefetching model cache transaction distributed storage systems LSTM neural network

The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology

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Genomics, Proteomics & Bioinformatics 2013年第1期11卷 34-40页

作者： Fei Chen Mengxing dong Meng Ge Lingxiang zhu Lufeng Ren Guocheng Liu Rong Mu CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of Sciences

DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of develop- ment, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the ＂REAP＂ method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application.

关键词： DNA polymerases Sequencing technology Modified nucleotide Primer extension Reversible terminator Sequencing by synthesis

基于叙事医学模式的健康管理对围绝经期脑卒中患者自我管理认知功能及肢体功能的影响

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中国妇幼保健 2024年第10期39卷 1883-1886页

作者：朱冬冬贾震雷李栩婷严丽陈秀娣廉长红连妙芬绍兴市上虞人民医院神经内科浙江绍兴312300

目的探讨基于叙事医学模式的健康管理对围绝经期女性脑卒中患者自我管理、认知功能及肢体功能的影响。方法选取2020年1月-2023年4月在绍兴市上虞人民医院就诊的围绝经期女性脑卒中患者100例为研究对象,随机将其分常规健康管理(HM)组... 详细信息

目的探讨基于叙事医学模式的健康管理对围绝经期女性脑卒中患者自我管理、认知功能及肢体功能的影响。方法选取2020年1月-2023年4月在绍兴市上虞人民医院就诊的围绝经期女性脑卒中患者100例为研究对象,随机将其分常规健康管理(HM)组和基于叙事医学模式的健康管理(NH)组,每组各50例,观察两组患者自我管理、认知功能及肢体功能等情况。结果干预前,两组自我健康管理量表评分比较差异无统计学意义(P>0.05);干预后,NH组自我健康管理量表评分[(3.66±0.88)分]高于HM组[(2.34±0.71)分],差异有统计学意义(t=8.255,P0.05);干预后,NH组MoCA评分[(27.15±2.59)分]高于HM组[(24.63±2.03)分],差异有统计学意义(t=5.415,P0.05);干预后,NH组总FMA评分[(71.94±7.43)分]高于HM组[(60.33±6.67)分],差异有统计学意义(t=8.222,P<0.05)。与HM组对比,NH组撞伤、窒息、再出血及舌咬伤人数明显降低,NH组不良事件总发生率(6.00%)明显低于HM组(24.00%),组间比较差异有统计学意义(χ^(2)=6.353,P<0.05)。结论采用基于叙事医学模式的健康管理对围绝经期女性脑卒中患者进行干预的效果更佳,可显著提高患者自我管理能力、认知功能及肢体功能。

关键词：叙事医学模式健康管理围绝经期脑卒中自我管理认知功能肢体功能