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PRKCQ-AS1通过Akt信号促进气道平滑肌细胞增殖的研究

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中国药理学通报 2025年第1期41卷 88-94页

作者：吴露依胡方启安庆医药高等专科学校临床科研中心安庆市立医院安徽安庆246000

目的探讨PRKCQ-AS1在气道平滑肌细胞(airway smooth muscle cells,ASMCs)的作用。方法首先通过转录组测序技术(RNA-seq)检测哮喘儿童肺泡灌洗液中的差异表达基因(differentially expressed genes,DEGs)。随后,分别构建PRKCQ-AS1慢病毒敲低和过表达载体并转染ASMCs。qRT-PCR方法检测了ASMCs中PRKCQ-AS1的表达;用CCK-8和流式细胞术分别检测ASMCs的增殖和凋亡;酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)检测ASMCs培养上清液中TNF-α和IL-1β的含量;lncRNA芯片检测PRKCQ-AS1敲低后的DEGs并进行KEGG通路富集;Western blot检测p-Akt和Akt的水平。结果RNA-seq结果显示,PRKCQ-AS1是上调最明显的DEGs之一。PRKCQ-AS1过表达促进了ASMCs的增殖和迁移并抑制了其凋亡,同时,PRKCQ-AS1过表达使上清液中炎性因子TNF-α和IL-1β的水平升高,而PRKCQ-AS1敲低则呈现相反的结果。对PRKCQ-AS1敲低后得到的606个DEGs进行KEGG信号通路富集分析,结果显示PI3K-Akt信号被明显富集。Western blot结果表明,PRKCQ-AS1敲低抑制了Akt的活化;相反,PRKCQ-AS1过表达则促进了Akt的水平。结论PRKCQ-AS1可能通过增强Akt信号促进ASMCs炎症反应,进而加剧了ASMCs的增殖和迁移,最终导致哮喘的发展。

关键词： PRKCQ-AS1 ASMCs 哮喘 Akt信号细胞实验炎性细胞因子

苹果褪绿叶斑病毒辽宁分离物生物学和基因组研究

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植物病理学报 2018年第5期48卷 716-720页

作者：张双纳李正男张尊平范旭东任芳胡国君董雅凤中国农业科学院果树研究所国家落叶果树脱毒中心辽宁兴城125100

苹果褪绿叶斑病毒（Apple chlorotic leaf spotvirus，ACLSV）属于乙型线形病毒科（Betaflexiviridae）纤毛病毒属（Trichovirus），基因组为正义单链RNA。含有约7500个核苷酸。编码3个部分重叠的开放阅读框。目前从GenBank数据库可以... 详细信息

苹果褪绿叶斑病毒（Apple chlorotic leaf spotvirus，ACLSV）属于乙型线形病毒科（Betaflexiviridae）纤毛病毒属（Trichovirus），基因组为正义单链RNA。含有约7500个核苷酸。编码3个部分重叠的开放阅读框。目前从GenBank数据库可以检索到19个ACLSV分离物的基因组序列。

关键词：苹果褪绿叶斑病毒基因组序列分离物 GenBank数据库生物学辽宁开放阅读框 ACLSV

Dynamic hybrid multimedia distribution scheme based on network reconfiguration

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Science China(Information Sciences) 2014年第10期57卷 107-116页

作者： dong fang hu YuXiang LAN JuLong National Digital Switching System Engineering and Technological Research Center

This paper addresses a key challenge in the design and implementation of multimedia distribution systems so that users can experience good performance with minimal operational costs. In current structures,the conflict between network architectures and service provision causes a decline in users’ expectations of quality. Network reconfiguration provides a feasible solution for dynamic distribution as a primitive for both quality and efficiency. In this paper, we first present a scheme for a layer-based hybrid multimedia distribution system LHMDS, and then introduce a dynamic sliding push-delivery mechanism based on reconfiguration of the distribution of hotspot files. The LHMDS adopts a unique structure with hybrid channels, including broadcast and unicast ones, and adjusts its distribution topology according to the congestion status, thereby pushing hotspot files dynamically. The mechanism dynamically pushes files to upper or lower network layers depending on the network status while distributing hotspot files by broadcast channels and other files by unicast *** guarantees service quality as well as improving resource utilization. Finally, we validate the feasibility and performance of the scheme through a simulation.

关键词： network reconfiguration hybrid distribution dynamic sliding push-delivery

指向高质量课堂的听评课量表设计及其关键问题

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教学与管理 2025年第1期 24-28页

作者：户艳茹方妍清华大学教育研究院北京100084 清华大学附属中学北京100084

在建设高质量课堂的时代背景下,重新设计课堂听评课框架与维度,有助于倒逼教师专业成长,反促课堂提质增效。在深刻反思现有听评课量表存在不足、尤其是缺乏科学量化标准的前提下,重新设计的旨在提升课堂质量的听评课量表主要包括2个观... 详细信息

在建设高质量课堂的时代背景下,重新设计课堂听评课框架与维度,有助于倒逼教师专业成长,反促课堂提质增效。在深刻反思现有听评课量表存在不足、尤其是缺乏科学量化标准的前提下,重新设计的旨在提升课堂质量的听评课量表主要包括2个观察维度、6个观察要素、13个观察指标和26个观察点。在教学实践中应用这一听评课量表,需要注意凸显学生的主体性、全面性与个性化,构建民主平等的课堂生命共同体,展现时代风格与学校特色,形成良性教研文化和沟通合作机制。

关键词：高质量课堂听评课量表教研制度

Research on disruptive design pre-identification in the preliminary design phase of aero engine flow pass multidisciplinary optimization

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Chinese Journal of Aeronautics 2022年第11期35卷 264-276页

作者： Shaojing dong Yufan fang Wentong hu Xiuli SHEN Gaoxiang CHEN School of Energyand Power Engineering Beihang UniversityBeijing 10083China Beijing Key Laboratory of Aero-Engine Structure and Strength Beihang UniversityBejing 100083China Jiangxi Research Institute Beihang UniversityNanchang 330096China Navigation and Control Technology Research Institute of China Ordnance Industries Beijing 100089China Research Institute of Aero-engine Beihang UniversityBeijing 100083China

In aero engine design, determining whether the preliminary design will have disruptive effects on the detailed design is the key to multidisciplinary design optimization in the preliminary design stage. In order to adapt to the non-orthogonal parameter value range caused by the selfconstrained parametric modeling method, a non-orthogonal space mapping method that maps the optimal Latin hypercube sampling points of the traditional orthogonal design space to the non-orthogonal design space is proposed. Based on the logical regression method in machine learning field, a kind of feasible domain boundary identification method is employed to identify whether the sample spatial response meets the relevant criteria. The method proposed in this paper is used to identify and analyze the key technologies of the high-pressure turbine mortise joint structure. It is found that the preliminary design of the aero engine may lead to the failure to obtain a mortise joint structure meeting the design requirements in the detailed design stage. The mortise joint structure needs to be pre-optimized in the preliminary design stage.

关键词： Aero engine Disruptive design Flow pass multidisciplinary optimization Pre-identification Preliminary design phase

Prediction of dissolved gas content in transformer oil based on multi-information fusion

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High Voltage 2024年第3期9卷 685-699页

作者： Tongliang Yang Yun fang Chengming Zhang Chao Tang dong hu College of Engineering and Technology Southwest UniversityChongqingChina International R&D Center for New Technologies of Smart Grid and Equipment Southwest UniversityChongqingChina

In order to accurately predict the content and variation trend of dissolved gas in trans-former oil and guide the condition maintenance of power transformers,a combined prediction model based on multi-information fusion is proposed and its effectiveness is *** of all,based on the possibility of pathological and missing historical sample data,a detection and filling method based on variable weight combination samples is ***,the authors propose two *** at the non-linear and non-stationary characteristics of gas content,a univariate decomposition prediction mode HBA-VMD-TCN which based on the Honey Badger algorithm,variational mode decomposition and time convolutional network(TCN)is *** the multi-variate Informer prediction model is established for gas content affected by multiple ***,the cross-entropy theory is used to determine the weight coefficients of the two models,and the multi-information fusion combined prediction model is ***,on the basis of the above,a method to determine the time step and the position information of the transition point adaptively in the process of prediction is proposed to further improve the prediction *** results show that,through a series of simulation experiments of model comparison and transformer anomaly prediction,the accuracy and effectiveness of the combined prediction model are verified.

关键词： content prediction dissolved

Efficient and simple synthesis of B_(2)O_(3)-Al_(2)O_(3)nanocomposites assisted by deep eutectic solvent for production of 5-hydroxymethylfurfural from glucose

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Nano Research 2025年第1期18卷 236-248页

作者： Yuchen Wang Kai Rong Shanlei Chang Youxing fang Shaojun dong State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China College of Chemistry Jilin UniversityChangchun 130012China

The catalytic conversion of glucose to high valueadded platform chemical 5-hydroxymethylfurfural(HMF)is a promising biorefinery process,and alumina-boria catalysts are considered to be green and mild solid acid catalysts for this catalytic ***,compared to the common synthesis methods with complicated steps,we reported a simple and efficient strategy to prepare B_(2)O_(3)-Al_(2)O_(3)nanocomposites by calcining cost-effective glucose-urea deep eutectic solvent(DES)solution containing the *** prepared B_(2)O_(3)-Al_(2)O_(3)nanocomposites exhibited an open three-dimensional skeleton and two-dimensional porous lamellar substructure,endowing them with a high specific surface area(228.27 m2/g).The introduction of boron changed the ratios of different aluminum species(AlⅣ,AlⅤ,and AlⅥ)and borate species(BO3 and BO4),thus further affecting the acidity and the types of acid sites of the *** prepared B_(2)O_(3)-Al_(2)O_(3)bifunctional acid catalysts possessing abundant Lewis acid sites and adjustable Brønsted acid sites showed complete glucose conversion and 55.38%of HMF yield under the optimum *** study proposed a concise method to synthesize alumina-boria solid acid catalysts assisted by glucose-urea *** hope to extend the application and prospect of this efficient and simple synthesis strategy.

关键词： 5-hydroxymethylfurfural(HMF) deep eutectic solvent glucose alumina-boria acid catalyst

Comparable charge transport property based on S…S interactions with that of π-π stacking in a bis-fused tetrathiafulvalene compound

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Science China Chemistry 2017年第4期60卷 510-515页

作者： Ke Zhou Hongfeng Chen huanli dong Qi fang Wenping hu Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China State Key Laboratory of Crystal Materials Shandong University Jinan 250100 China Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Department of Chemistry School of Science Tianjin University Tianjin 300072 China

Compact molecular packing with short π-π stacking and large π-overlap in organic semiconductors is desirable for efficient charge transport and high carrier mobility. Thus charge transport anisotropy along different directions is commonly observed in organic semiconductors. Interestingly, in this article, we found that comparable charge transport property were achieved based on the single crystals of a bis-fused tetrathiafulvalene derivative （EM-TTP） compound along two interaction directions, that is, the multiple strong S…S intermolecular interactions and the π-π stacking direction, with the measured electrical conductivity and hole mobility of 0.4 S cm-1, 0.94 cm2 V i s 1 and 0.2 S cm-1, 0.65 cm2 V-1 s-1, respectively. This finding provides us a new molecular design concept for developing novel organic semiconductors with isotropic charge transport property through the synergistic effect of multiple intermolecular interactions （such as π-π interactions） and π-π stacking.

关键词： bis-fused TTF derivative single crystal electrical conductivity hole carrier mobility

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数字技术驱动下中国民歌变革及应对策略研究

湖北科技学院学报 2025年第1期45卷 80-85页

作者：胡娟方元河南职业技术学院音乐学院河南郑州450046

数字科技的崛起对音乐创作和传播方式带来了革命性的变化,同时也对中国民歌的传承和发展提出了新的课题。本研究旨在深入探讨数字化发展对中国民歌的影响,包括作品表演形式与作品的传承与创新,并详细分析其所面临的挑战和机遇,提出相应... 详细信息

数字科技的崛起对音乐创作和传播方式带来了革命性的变化,同时也对中国民歌的传承和发展提出了新的课题。本研究旨在深入探讨数字化发展对中国民歌的影响,包括作品表演形式与作品的传承与创新,并详细分析其所面临的挑战和机遇,提出相应的对策和建议。通过理论支持和实践指导,期望为传承和创新中国民歌作出积极贡献。

关键词：数字技术中国民歌变革应对