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Annexin A2 silencing enhances apoptosis of human umbilical vein endothelial cells in vitro

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Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 929-933页

作者： Shu-Le Jiang dong-yan pan Chao Gu Hai-Feng Qin Shi-Hong Zhao Department of Ophthalmology Affiliated Changhai Hospital of Second Military Medical University

Objective:To study the effects of inhibited Annexin A2(ANXA2) on human umbilical vein endothelial cells(HUVECs) in ***:Short hairpin RNA(shRNA) targeting ANXA2 was designed and cloned into double marked lentvirial vector GV248 for RNAi to generate the recombinant expression plasmids,which were stably transfected into *** protein and mRNA expression levels of ANXA2 were analyzed by western blotting and realtime polymerase chain reaction,*** proliferation(cell counting kit-8 assay),apoptosis(flow cytometry analysis),the expression(western blotting) and the activity of easpases(enzyme-linked immunosorbent assay) were used to assess the effects of silencing ANXA2 on HUVECs in ***:The plasmids to express ANXA2-specific shRNA were constructed and were infected into HUVEC resulting in the stably transfected experimental(ANXA2-shRNA),control(eontrol-shRNA) and mock(no plasmid) cell lines,which were verified with western blot and real-time ***/ANXA2-shRNA showed an inhibition rate 91.89%of ANXA2 expression compared to the mock ***2 silencing cell strain obviously presented a lower cell proliferation activity compared to the control and mock HUVECs,with an inhibition rate 82.35%on day 7 in *** analysis indicated that the HUVEC/ANXA2-shRNA cells undergoing apoptosis increased by 102.61%compared to the mock HUVECs(P<0.01).Moreover,the activity levels of caspase-3,caspase-8 and caspase-9in HUVEC/ANXA2-shRNA cells were increased and the activated cleaved caspase-3,cleaved Caspase-8 and cleaved caspase-9 were upregulated evidently compared with that of the control and mock HUVECs by 56.29%,89.59%and 144.58%(P<0.01).Conclusions:shRNAmediated silencing of ANX A2 could not only be able to suppress HUVECs:proliferation but to upregulate the enzyme activity of easpases,which bring to an increase of cell apoptosis This work suggested that ANX A2 may represent a useful target of future molecular therapies.

关键词： Retinal neovascularization Annexin A2 RNA interfer

DNA damage response-related immune activation signature predicts the response to immune checkpoint inhibitors: from gastrointestinal cancer analysis to pan-cancer validation

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Cancer Biology & Medicine 2024年第3期21卷 252-266页

作者： Junya yan Shibo Wang Jing Zhang Qiangqiang Yuan Xianchun Gao Nannan Zhang yan pan Haohao Zhang Kun Liu Jun Yu Linbin Lu Hui Liu Xiaoliang Gao Sheng Zhao Wenyao Zhang Abudurousuli Reyila Yu Qi Qiujin Zhang Shundong Cang Yuanyuan Lu yanglin pan yan Kong Yongzhan Nie State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases Xijing Hospital of Air Force Military Medical UniversityXi'an 710032China Department of Oncology Henan Provincial People's HospitalZhengzhou University People's HospitalZhengzhou 450o03China Faculty of Life Science Northwest UniversityXi'an 710069China Unit 73211 of the People's Liberation Army Nanjing 211800China Key Laboratory of Resource Biology and Biotechnology in Western China(Ministry of Education) School of MedicineNorthwest UniversityXi'an 710069China Shaanxi University of Chinese Medicine Second Clinical Medicine FacultyXi'an 712046China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Renal Cancer and MelanomaPeking University Cancer Hospital and InstituteBeijing 100142China

Objective: DNA damage response(DDR) deficiency has emerged as a prominent determinant of tumor immunogenicity. This study aimed to construct a DDR-related immune activation(DRIA) signature and evaluate the predictive accuracy of the DRIA signature for response to immune checkpoint inhibitor(ICI) therapy in gastrointestinal(GI) ***: A DRIA signature was established based on two previously reported DNA damage immune response assays. Clinical and gene expression data from two published GI cancer cohorts were used to assess and validate the association between the DRIA score and response to ICI therapy. The predictive accuracy of the DRIA score was validated based on one ICI-treated melanoma and three pan-cancer published ***: The DRIA signature includes three genes(CXCL10, IDO1, and IFI44L). In the discovery cancer cohort, DRIA-high patients with gastric cancer achieved a higher response rate to ICI therapy than DRIA-low patients(81.8% vs. 8.8%;P pancreatic adenocarcinoma who received dual PD-1 and CTLA-4 blockade with radiation therapy. Furthermore, the predictive performance of the DRIA score was shown to be robust through an extended validation in melanoma, urothelial cancer, and ***: The DRIA signature has superior and robust predictive accuracy for the efficacy of ICI therapy in GI cancer and pancancer, indicating that the DRIA signature may serve as a powerful biomarker for guiding ICI therapy decisions.

关键词： DNA damage response-related immune activation immune checkpoint inhibitors biomarker gastrointestinal cancer pan-cancer

Recovering from prolonged cardiac arrest induced by electric shock:A case report

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World Journal of Clinical Cases 2024年第13期12卷 2248-2253页

作者： Jian Zhang yan-Ru Qiao Ya-dong yang Guo-Zheng pan Chong-Qing Lv Department of General Surgery Shengli Oilfield Central HospitalDongying 257000Shandong ProvinceChina Department of Emergency Medicine Shengli Oilfield Central HospitalDongying 257000Shandong ProvinceChina

BACKGROUND Cardiac arrest(CA)induced by electric shock is a rare occurrence,particularly in cases of prolonged ***,there is limited literature on similar incidents,and we present a relevant case *** SUMMARY A 27-year-old Asian male man,experiencing respiratory CA due to electric shock,was successfully restored to sinus rhythm after 50 min of cardiopulmonary resuscitation and 8 electrical defibrillation *** the subsequent stages,the patient received multiple organ function protection measures,leading to a successful recovery and eventual discharge from the *** Prolonging resuscitation time can enhance the chances of survival for patients,this study provide valuable insights into the management of electric shock-induced CA.

关键词： Electric shock Cardiac arrest Prolonged cardiopulmonary resuscitation Cerebral resuscitation Case report

Drug delivery systems for colorectal cancer chemotherapy

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Chinese Chemical Letters 2024年第2期35卷 163-175页

作者： Wen Chen Kun Shi yan Yu Peipei yang Zhongwu Bei dong Mo Liping Yuan Meng pan Yu Chen Zhiyong Qian Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China

Colorectal cancer causes the third most common type of malignant tumors with high morbidity and *** is currently one of the most effective and common treatments for colorectal ***,the poor water solubility of some chemotherapeutics,untargeted drug delivery,and the undesirable systemic side effects of conventional treatment remain the major issues for colorectal cancer ***,drug delivery systems(DDS)based on biomaterials have been widely investigated and found to be capable of resolving those issues with good ***,the main goal of this review is to summarize and discuss the progress and potential advantages of different DDS for colorectal cancer *** not only reviewed the nanocarriers used to improve the solubility of chemotherapeutics,including liposomes,micelles,and nanoparticles,but also discussed targeted DDS based on specific ligand-receptor recognition and tumor microenvironmental stimulus ***,locally administered systems based on hydrogels and microspheres,which have been shown to increase drug accumulation at the tumor site while decreasing systemic toxicity,were also *** provides a good option for improving the efficacy of chemotherapy in the treatment of colorectal cancer.

关键词： Colorectal cancer Chemotherapy Drug delivery systems Solubility Targeted Local administration

Link between childhood obesity and gut microbiota

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World Journal of Gastroenterology 2024年第30期30卷 3560-3563页

作者： yan pan Fu-Yong Jiao Department of Pediatrics The First Affiliated Hospital of Yangtze UniversityJingzho 434000Hubei ProvinceChina Shaanxi Kawasaki Disease Diagnosis and Treatment Center Children's HospitalShaanxi Provincial People's Hospital of Xi'anJiaotong UniversityXi'an 710000Shaanxi ProvinceChina

Childhood obesity is a critical global health concern with rising prevalence and significant long-term health *** studies have implicated gut microbiota in the development and progression of *** editorial analyzes the research conducted by Li et al,who utilized 16S rRNA gene sequencing to compare the gut microbiome of overweight and healthy-weight *** study found significant differences in microbial diversity and composition between the two groups,with potential implications for understanding and managing childhood *** analyzed the study’s advantages and drawbacks,proposing potential areas for future research to better understand the connection between gut microbiota and obesity.

关键词： 16S rRNA Childhood obesity Gut microbiota Future research Global health

Hepatocyte growth factor promotes retinal pigment epithelium cell activity through MET/AKT signaling pathway

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 806-814页

作者： Si-Rui Zhou Yu-Sheng Zhu Wen-Ting Yuan Xiao-yan pan Tong Wang Xiao-dong Chen Faculty of Life Sciences and Medicine Northwest UniversityXi’an 710069Shaanxi ProvinceChina First Affiliated Hospital of Northwest University Northwest UniversityXi’an 710069Shaanxi ProvinceChina Department of Ophthalmology Xi’an No.1 HospitalXi’an 710002Shaanxi ProvinceChina Shaanxi Institute of Ophthalmology Shaanxi Provincial Key Lab of OphthalmologyClinical Research Center for Ophthalmology Diseases of Shaanxi ProvinceXi’an 710002Shaanxi ProvinceChina

AIM:To explore the effects of hepatocyte growth factor(HGF)on retinal pigment epithelium(RPE)cell ***:The human adult retinal pigment epithelial cell line-19(ARPE-19)were treated by HGF or mesenchymalepithelial transition factor(MET)inhibitor SU11274 in *** viability was detected by a Cell Counting Kit-8 *** proliferation and motility was detected by a bromodeoxyuridine incorporation assay and a wound healing assay,*** expression levels of MET,phosphorylated MET,protein kinase B(AKT),and phosphorylated AKT proteins were determined by Western blot *** MET and phosphorylated MET proteins were also determined by immunofluorescence ***:HGF increased ARPE-19 cells’viability,proliferation and migration,and induced an increase of phosphorylated MET and phosphorylated AKT ***11274 significantly reduced cell viability,proliferation,and migration and decreased the expression of MET and AKT ***11274 suppressed HGF-induced increase of viability,proliferation,and migration in ARPE-19 ***,SU11274 also blocked HGF-induced phosphorylation of MET and AKT ***:HGF enhances cellular viability,proliferation,and migration in RPE cells through the MET/AKT signaling pathway,whereas this enhancement is suppressed by the MET inhibitor ***-induced MET/AKT signaling might be a vital contributor of RPE cells survival.

关键词： hepatocyte growth factor mesenchymal epithelial transition factor SU11274 retinal pigment epithelial cells

Efficacy of scraping therapy on blood pressure and sleep quality in stage Ⅰ and Ⅱ essential hypertension: A systematic review and meta-analysis

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Journal of Integrative Medicine 2024年第1期22卷 12-21页

作者： Zheng-gang Zhu Jian-ru Wang Xiao-yan pan School of Nursing Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina School of Health Sciences Universiti Sains MalaysiaKubang Kerian 16150KelantanMalaysia Department of Cardiovascular Medicine the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 450000Henan ProvinceChina

Background Scraping therapy is widely used in treating stage I and II essential hypertension in ***,there has been no systematic evaluation of the efficacy of scraping therapy on blood pressure and sleep quality in stage I and II essential *** strategy Seven electronic databases(PubMed,Scopus,Cochrane Library,Web of Science,EBSCO,China National Knowledge Infrastructure and Wanfang Data electronic databases)were searched from inception to December *** on the principle of combining subject words with text words,the search strategy was constructed around search terms for“scraping therapy,”“scraping,”“Guasha,”“Gua sha,”“hypertension,”and“high blood pressure”during the database *** criteria Randomized controlled trials(RCTs)were included if they recruited patients with stage I and II essential hypertension and included a scraping therapy *** intervention group received antihypertensive drugs and scraping therapy,while the control group only took antihypertensive *** extraction and analysis Review Manager 5.4.0 and STATA 15.1 were used to enter all the relevant outcome variables to conduct the *** quality of the selected RCTs was assessed using the PEDro *** sensitivity analysis was carried out by iteratively excluding individual studies and repeating the analysis to determine the stability of the findings and identify any studies with greater influence on the *** analysis was performed to find the source of *** plots were used to evaluate the publication bias of included *** Nine RCTs including 765 participants were ***-analysis showed that scraping therapy combined with medication had an advantage over the use of medication alone in lowering systolic blood pressure(mean difference[MD]=?5.09,95%confidence interval[CI]=?6.50 to?3.67,P<0.001)and diastolic blood pressure(MD=?2.66,95%CI=?3.17 to?2.14,P<0.001).Subgroup analysis showed tha

关键词： Scraping therapy Essential hypertension Efficacy Blood pressure Sleep quality Systematic review Meta-analysis

Mass transfer enhancement and hydrodynamic performance with wire mesh coupling solid particles in bubble column reactor

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Chinese Journal of Chemical Engineering 2024年第3期67卷 195-205页

作者： Chuanjun Di Jipeng dong Fei Gao Guanghui Chen pan Zhang Jianlong Li Key Laboratory of Multiphase Flow Reaction and Separation Engineering of Shandong Province College of Chemical EngineeringQingdao University of Science and TechnologyQingdao 266042China College of Electromechanical Engineering Qingdao University of Science and TechnologyQingdao 266043China

It is of vital significance to investigate mass transfer enhancements for chemical engineering *** work focuses on investigating the coupling influence of embedding wire mesh and adding solid particles on bubble motion and gas-liquid mass transfer process in a bubble *** image velocimetry(PIV)technology was employed to analyze the flow field and bubble motion behavior,and dynamic oxygen absorption technology was used to measure the gas-liquid volumetric mass transfer coefficient(kLa).The effect of embedding wire mesh,adding solid particles,and wire mesh coupling solid particles on the flow characteristic and kLa were analyzed and *** results show that the gas-liquid interface area increases by 33%-72%when using the wire mesh coupling solid particles strategy compared to the gas-liquid two-phase flow,which is superior to the other two strengthening *** with the system without reinforcement,kLa in the bubble column increased by 0.5-1.8 times with wire mesh coupling solid particles method,which is higher than the sum of kLa increases with inserting wire mesh and adding particles,and the coupling reinforcement mechanism for affecting gas-liquid mass transfer process was discussed to provide a new idea for enhancing gas-liquid mass transfer.

关键词： Fluid mechanics Bubble Mass transfer Wire mesh coupling solid particles Particle image velocimetry Hydrodynamics

Regulated reaction pathway of CO2 photoreduction into CH4 by metal atom pair sites

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Rare Metals 2024年第9期43卷 4620-4627页

作者： dong-Po He Guang-Bing Huang Jun Hu yang Wu Xiao-dong Li Qing-Xia Chen Shan Zhu Wen-Sheng yan Jun-Fa Zhu yang pan Xing-Chen Jiao Key Laboratory of Synthetic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan University Hefei National Research Center for Physical Sciences at Microscale National Synchrotron Radiation LaboratoryUniversity of Science and Technology of China Max Planck Institute of Microstructure Physics State Grid Anhui Electric Power Research Institute

The carbon dioxide （CO2） reduction process involves complex protonation, making the resulting product often unpredictable. To achieve the desired product, it is crucial to manipulate the reaction steps. Herein, we build the metal atom pair sites for selective CO2photoreduction into methane. As a prototype, Ni atom pair sites loaded on the Mo S2nanosheets were synthesized and verified by highresolution transmission electron microscopy （HRTEM）,X-ray photoelectron spectroscopy （XPS） and X-rayabsorption near edge structure spectra （XANES）. In-situ Fourier transform infrared spectroscopy （FTIR） monitors the*CHO group, a crucial intermediate in CH4production,during CO2photoreduction on the Ni-Mo S2nanosheets,whereas this monitoring is not observed for the Mo S2nanosheets. Also, theoretical calculations disclose that over the Ni-Mo S2nanosheet slab, the formation energy of*CHO intermediates is determined to be lower （0.585 e V） than the desorption energy of*CO intermediates for CO production（0.64 e V）, implying the higher selectivity of CH4production. Accordingly, the Ni-Mo S2nanosheets demonstrate a methane formation rate of 27.21μmol·g-1·h-1, coupled with an impressive electron selectivity of 94.2%.

关键词： Nanosheets