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A preliminary study of dietary protein requirement of juvenile marbled flounder (Pseudopleuronectes yokohamae)

Animal Nutrition 2021年第2期7卷 548-555页

作者： Jeong-Hyeon Cho Seunghyung Lee Bong-Joo Lee Sang-Woo Hur Kang-Woong Kim Maeng-Hyun Son dong-jae yoo Inland Fisheries Research Institute National Institute of Fisheries ScienceGapyeong12453South Korea Department of Marine Bio-materials and Aquaculture Pukyong National UniversityBusan48513South Korea Aquafeed Research Center National Institute of Fisheries SciencePohang37517South Korea Gyeongsangbuk-do Fisheries Resources Institute Yeongdeok36405South Korea Gyeongsangbuk-do Native Fish Business Center Uiseong37366South Korea

An 8-week feeding trial was conducted to determine the optimal dietary protein level for juvenile marbled flounder.Five semi-purified test diets were formulated to contain different protein levels(CP)including 42.7%,47.4%,53.3%,58.8%,and 64.5%(dry matter),named as CP42.7,CP47.4,CP53.3,CP58.8,and CP64.5,respectively.Five hundred and twenty-five juveniles(6.0±0.1 g)were randomly distributed into 15 tanks(300 L tanks),resulting in 35 fish per tank(n-3 tanks).Fish were fed the test diets 5 times per day until satiation.The CP58.8 resulted in the highest gain in weight and the best efficiency in feed utilization among the tested protein levels(P<0.05).Fish fed the CP58.8 diet showed significantly higher whole-body protein and lipid contents than the fish that were fed the other diets(P<0.05).Fish fed the CP53.3,CP58.8,and CP64.5 diets showed a significantly higher dorsal-muscle lipid content than the fish that were fed the CP42.7 and CP47.4 diets(P<0.05).The one-slope straight broken-line regression analysis on the results of the thermal growth coefficient and feed conversion ratio indicated that the estimated optimum dietary protein level was 58.8%.Taken together,it is suggested that the dietary protein level of 58.8%is optimal for better growth and high efficiency in feed utilization for the juvenile marbled flounder.

关键词： Marbled flounder Pseudopleuronectes yokohamae Protein requirement Growth performance Proximate composition

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Novel extended C-m models of flow stress for accurate mechanical and metallurgical calculations and comparison with traditional flow models

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Journal of Magnesium and Alloys 2022年第9期10卷 2516-2533页

作者： Man Soo Joun Mohd Kaswandee Razali jae dong yoo Min Cheol Kim Jeong Muk Choi ReCAFT Gyeongsang National UniversityGraduate School of Mechanical and Aerospace EngineeringJinju 52828South Korea Gyeongsang National University Graduate School of Mechanical and Aerospace EngineeringJinju 52828South Korea MFRC Research CenterJinju 52818South Korea Jinhap Daejeon 34302South Korea

Here,we developed novel extended piecewise bilinear power law(C-m)models to describe flow stresses under broad ranges of strain,strain rate,and temperature for mechanical and metallurgical calculations during metal forming at elevated temperatures.The traditional C-m model is improved upon by formulating the material parameters C and m,defined at sample strains and temperatures as functions of the strain rate.The coefficients are described as a linear combination of the basis functions defined in piecewise patches of the sample strain and temperature domain.A comparison with traditional closed-form function flow models revealed that our approach using the extended piecewise bilinear C-m model is superior in terms of accuracy,ease of use,and adaptability;additionally,the extended C-m model was applicable to numerical analysis of mechanical,metallurgical,and microstructural problems.Moreover,metallurgy-related values can be calculated directly from the flow stress information.Although the proposed model was developed for materials at elevated temperatures,it can be applied over a broad temperature range.

关键词： Flow stress Power law model Piecewise bilinear function Numerical analysis Microstructural prediction Peak strain

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Comparative efficacy and optimal duration of first-line antibiotic regimens for acute otitis media in children and adolescents:a systematic review and network meta-analysis of 89 randomized clinicaltrials

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World Journal of Pediatrics 2024年第3期20卷 219-229页

作者： Min Seo Kim jae Han Kim Seohyun Ryu Seung Won Lee dong Keon Yon Eunyoung Kim Ai Koyanagi Elena Dragioti jae Il Shin Lee Smith Samsung Advanced Institute forHealth Sciences&Technology(SAIHST) Samsung Medical CenterSungkyunkwan UniversitySeoulRepublic of Korea Yonsei University Collegeof Medicine SeoulRepublicof Korea Department of Precision Medicine Sungkyunkwan University School of MedicineSuwonRepublic of Korea Center for Digital Health Medical Science Research InstituteKyung Hee University College of MedicineSeoulRepublic of Korea Evidence-Based Research Laboratory Department of Clinical Pharmacy and Pharmaceutical CareCollege of PharmacyChung-Ang UniversitySeoulRepublic of Korea Parc Sanitari Sant Joan de Deu/CIBERSAM/ISCIII Universitat de BarcelonaFundacio Sant Joan de Déu.Sant Boi de LlobregatBarcelonaSpain ICREA Pg.Lluis Companys 23BarcelonaSpain Pain and Rehabilitation Centre and Department of Medical and Health SciencesLinkoping University58185 LinkopingSweden Department of Pediatrics Yonsei University College of MedicineYonsei-ro 50Seodaemun-gu8044Seoul 03722Republic of Korea Centre for Health Performanceand WellbeingAnglia Ruskin UniversityCambridge CB11PTUK Research Laboratory Psychology of Patients Families&Health ProfessionalsDepartment of NursingSchool of Health SciencesUniversity of IoanninaIoannina 45500Greece

Introduction Antibiotic use for acute otitis media(AOM)is one of the major sources of antimicrobial resistance.However,the effective minimal antibiotic duration for AOM remains unclear.Moreover,guidelines often recommend broad ranges(5-10 days)of antibiotic use,yet the clinical impact of such a wide window has not been assessed.Methods We systematically searched PubMed/MEDLINE,Embase,Scopus,Web of Science,and Cochrane Library from database inception to 6 October 2021.Network meta-analysis was conducted on randomized controlled trials that assessed antibiotic treatment for AOM in children(PROSPERO CRD42020196107).Results For amoxicillin and amoxicillin-clavulanate,7-day regimens were noninferior to 10-day regimens in clinical responses[amoxicillin:risk ratio(RR)0.919(95%CI 0.820-1.031),amoxicillin-clavulanate:RR 1.108(0.957-1.282)],except for≤2 years.For the third-generation cephalosporins,7-day and 10-day regimens had similar clinical responses compared to placebo[7-day:RR 1.420(1.190-1.694),10-day:RR 1.238(1.125-1.362)compared to placebo].However,5-day regimens of amoxicillin-clavulanate and third-generation cephalosporins were inferior to 10-day regimens.Compared to amoxicillin,a shorter treatment duration was tolerable with amoxicillin-clavulanate.Conclusions Our findings indicated that 1O days of antibiotic use may be unnecessarily long,while the treatment duration should be longer than 5 days.Otherwise,5-day regimens would be sufficient for a modest treatment goal.Our findings revealed that the current wide range of recommended antibiotic durations may have influenced the clinical outcome of AOM,and a narrower antibiotic duration window should be re-established.

关键词： Amoxicillin-potassium Amoxicillin Antibacterial agents Cephalosporins Duration of therapy

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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural fields.However,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising cost.Furthermore,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in cattle.In this point of view,electroporation in embryos has been risen as an alternative.Results First,editing efficiency of our electroporation methods were tested for embryos.Presence of mutation on embryo was confirmed by T7E1 assay.With first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,respectively.In case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for BLG.Afterwards,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep sequencing.Thirteen recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double KO.Ten surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both genes.Conclusions These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively applied.Finally,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Mixed-dimensional stacked nanocomposite structures for a specific wavelength-selectable ambipolar photoresponse

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Nano Research 2024年第6期17卷 5549-5558页

作者： Young jae Park jaeho Shim Joo Song Lee Kyu Seung Lee Ji-Yeon Kim Kang Bok Ko Sang-Youp Yim Seongjun Kim Hoon-Kyu Shin donghee Park Yong Ju Yun dong Ick Son Institute of Advanced Composite Materials Korea Institute of Science and TechnologyJeollabuk-do 55324Republic of Korea National Institute for Nanomaterials Technology Pohang University of Science and TechnologyGyeongsangbuk-do 37673Republic of Korea Department of Semiconductor Science and Technology Semiconductor Physics Research CenterJeonbuk National UniversityJeonju 54896Republic of Korea Advanced Photonics Research Institute Gwangju Institute of Science and TechnologyGwangju 61005Republic of Korea Center for Opto-Electronic Materials and Devices Post-Silicon Semiconductor InstituteKorea Institute of Science and TechnologySeoul 02792Republic of Korea Graduate School of Energy and Environment(KU-KIST Green School) Korea UniversitySeoul 02841Republic of Korea KIST School Department of Nanomaterials and Nano ScienceUniversity of Science and Technology(UST)Daejeon 34113Republic of Korea

Mixed-dimensional composite structures using zero-dimensional(0D)quantum dots(QDs)and two-dimensional(2D)transition metal dichalcogenides(TMDs)materials are expected to attract great interest in optoelectronics due to the potential to generate new optical properties.Here,we report on the unique optical characteristics of a devices with mixed dimensional vertically stacked structures based on tungsten diselenide(WSe_(2))/CdSeS QDs monolayer/molybdenum disulfide(MoS_(2))(2D/0D/2D).Specifically,it exhibits an ambipolar photoresponse characteristic,with a negative photoresponse observed in the 400-600 nm wavelength range and a positive photoresponse appeared at 700 nm wavelength.It resulted in the high negative responsivity of up to 52.22 mA·W^(−1)under 400 nm,which is 163 times higher than that of the photodetector without CdSeS QDs.We also demonstrated the negative photoresponse,which could be due to increased carrier collision probability and non-radiative recombination.Device modeling and simulation reveal that Auger recombination among the types of non-radiative recombination is the main cause of negative photocurrent generation.Consequently,we discovered ambipolar photoresponse near a specific wavelength corresponding to the energy of quantum dots.Our study revealed interesting phenomenon in the mixed low-dimensional stacked structure and paved the way to exploit it for the development of innovative photodetection materials as well as for optoelectronic applications.

关键词： mixed-dimension quantum dot heterostructure photodetector ambipolar photoresponse

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Surface-functionalized hole-selective monolayer for high efficiency single-junction wide-bandgap and monolithic tandem perovskite solar cells

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Journal of Energy Chemistry 2024年第1期88卷 317-326,I0008页

作者： Devthade Vidyasagar Yeonghun Yun jae Yu Cho Hyemin Lee Kyung Won Kim Yong Tae Kim Sung Woong Yang Jina Jung Won Chang Choi Seonu Kim Rajendra Kumar Gunasekaran Seok Beom Kang Kwang Heo dong Hoe Kim jaeyeong Heo Sangwook Lee School of Materials Science and Engineering Kyungpook National UniversityDaegu 41566Republic of Korea Department of Materials Science and Engineering and Optoelectronics Convergence Research CenterChonnam National UniversityGwangju 61186Republic of Korea Department of Materials Science and Engineering Korea UniversitySeoul 02841Republic of Korea Department of Nanotechnology and Advanced Materials Engineering Sejong UniversitySeoul 05006Republic of Korea

Carbazole moiety-based 2PACz([2-(9H-carbazol-9-yl)ethyl]phosphonic acid)self-assembled monolayers(SAMs)are excellent hole-selective contact(HSC)materials with abilities to excel the charge-transferdynamics of perovskite solar cells(PSCs).Herein,we report a facile but powerful method to functionalize the surface of 2PACz-SAM,by which reproducible,highly stable,high-efficiency wide-bandgap PSCs can be obtained.The 2PACz surface treatment with various donor number solvents improves assembly of 2PACz-SAM and leave residual surface-bound solvent molecules on 2PACz-SAM,which increases perovskite grain size,retards halide segregation,and accelerates hole extraction.The surface functionalization achieves a high power conversion efficiency(PCE)of 17.62%for a single-junction wide-bandgap(~1.77 e V)PSC.We also demonstrate a monolithic all-perovskite tandem solar cell using surfaceengineered HSC,showing high PCE of 24.66%with large open-circuit voltage of 2.008 V and high fillfactor of 81.45%.Our results suggest this simple approach can further improve the tandem device,when coupled with a high-performance narrow-bandgap sub-cell.

关键词： Perovskite solar cells 2PACz Monolithic tandem solar cells Wide bandgap

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黄海大海洋生态系滨海湿地保护:进展与建议

国家公园（中英文）

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国家公园（中英文） 2024年第4期2卷 272-280页

作者：曲方圆 jae-Won yoo 王守强自然资源部第一海洋研究所渤海海峡生态通道野外科学观测研究站青岛266061 Korea Institute of Coastal Ecology Inc. Bucheon 14449

黄海大海洋生态系(YSLME)滨海湿地是东亚—澳大利西亚候鸟的中转站和越冬栖息地,对东亚-澳大利西亚迁徙路线的候鸟至关重要。由于过去几十年的经济发展,中国和韩国都开垦了大量的滨海湿地,因此生境丧失被确定为是对YSLME的一个重大跨界... 详细信息

黄海大海洋生态系(YSLME)滨海湿地是东亚—澳大利西亚候鸟的中转站和越冬栖息地,对东亚-澳大利西亚迁徙路线的候鸟至关重要。由于过去几十年的经济发展,中国和韩国都开垦了大量的滨海湿地,因此生境丧失被确定为是对YSLME的一个重大跨界威胁。为缓解这一跨界威胁,YSLME二期项目在其战略行动计划中提出数项管理行动计划,同时,旨在2030年前保护30%海洋的全球生物多样性保护目标(3030目标)也得到了通过。因此,重点介绍了中国和韩国在湿地保护法律法规、滨海湿地保护地建设、公众意识以及国际合作等方面的进展,并简要介绍了朝鲜的滨海湿地保护情况,为更好地解决滨海湿地丧失这一跨界威胁和实现3030目标提供了建议。YSLME滨海湿地保护主要进展为:中韩两国逐渐完善其湿地保护法律法规体系、早期滨海湿地面积急剧丧失的趋势在近年来得到缓解、两国都建立了越来越多的滨海湿地保护地、提升公众意识已经体现在各级法律法规中、中韩之间有持续的滨海湿地国际合作交流活动。对于未来的保护工作,提出了四点建议:扩大滨海湿地保护地面积、保护迁徙动物的生态廊道、加强YSLME滨海湿地保护地网络建设以及增强保护地信息的可获得性。

关键词：黄海大海洋生态系滨海湿地保护进展建议

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Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles

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Asian Pacific Journal of Tropical Biomedicine 2024年第2期14卷 47-54页

作者： Hoibin Jeong dong-joo Lee Sung-Pil Kwon SeonJu Park Song-Rae Kim Seung Hyun Kim jae-Il Park Deug-chan Lee Kyung-Min Choi WonWoo Lee Ji-Won Park Bohyun Yun Su-Hyeon Cho Kil-Nam Kim Seoul Center Korea Basic Science InstituteSeoul 02841Republic of Korea Chuncheon Center Korea Basic Science InstituteChuncheon 24341Republic of Korea Research&Development Center Chungdam CDC JNPharm LLC.Chuncheon 24341Republic of Korea Department of Biomedical Technology Kangwon National UniversityChuncheon 24341Republic of Korea Yonsei Institute of Pharmaceutical Sciences College of PharmacyYonsei UniversityIncheon 21983Republic of Korea Gwangju Center Korea Basic Science InstituteGwangju 61751Republic of Korea Honam National Institute of Biological Resources 99Gohadoan-gilMokpo-siJeollanam-do58762Republic of Korea Department of Bio-analysis Science University of Science&TechnologyDaejeon34113Republic of Korea

Objective:To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in vivo.Methods:Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Cell proliferation was assessed using a 5-bromo-2’-deoxyuridine(BrdU)assay kit.Western blot analysis was performed to determine the protein expressions of related factors.The effects of Catalpa bignonioides extract were investigated in mice using the treadmill exhaustion test and whole-limb grip strength assay.Chemical composition analysis was performed using high-performance liquid chromatography(HPLC).Results:Catalpa bignonioides extract increased the proliferation of C2C12 mouse myoblasts by activating the Akt/mTOR signaling pathway.It also induced metabolic changes,increasing the number of mitochondria and glucose metabolism by phosphorylating adenosine monophosphate-activated protein kinase.In an in vivo study,the extract-treated mice showed improved motor abilities,such as muscular endurance and grip strength.Additionally,HPLC analysis showed that vanillic acid may be the main component of the Catalpa bignonioides extract that enhanced muscle strength.Conclusions:Catalpa bignonioides improves exercise performance through regulation of growth and metabolism in skeletal muscles,suggesting its potential as an effective natural agent for improving muscular strength.

关键词： Catalpa bignonioides Skeletal muscle Cell proliferation Mitochondria Energy metabolism C2C12

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Application of Transformed Two-Stage Network DEA to Strategic Design of Biofuel Supply Chain Network

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Journal of Systems Science and Systems Engineering 2023年第2期32卷 129-151页

作者： jae-dong Hong Industrial Engineering Program South Carolina State UniversityOrangeburgSCUSA

This paper proposes an innovative procedure for designing efficient biomass-biofuel logistics networks(BBLNs).This procedure is based on the two-stage network data envelopment analysis(TSN-DEA)models that have been developed to provide specific process guidance for the managers to improve the efficiency of the decision-making unit(DMU)with the TSN process.The crucial issue of the TSN-DEA is that the overall efficiency score depends on the DMUs under evaluation.Thus,the rankings for the DMUs generated by the TSN-DEA model are inconsistent.As a result,the TSN-DEA-based ranking methods are limited.The TSN-DEA’s inconsistency frequently makes it difficult for decision-makers to select the top-rated DMUs.We develop the transformed TSN(T-TSN)DEA method by applying the multi-criteria DEA model to overcome this issue.The proposed method transforms the DMUs with any number of inputs,intermediate measures,and outputs in the TSN process,through the multi-objective programming model with a minimax objective approach,into the DMUs with two inputs and one output in the single-stage network(SSN)process.Then,the well-known DEA methods for the SSN,such as the cross-efficiency and super-efficiency DEA methods,can be applied to evaluate and rank the transformed DMUs more consistently.We exhibit the applicability of the proposed approach for the BBLN design problem.A case study of South Carolina in the USA demonstrates that the proposed method performs well in identifying efficient BBLN schemes more consistently than the traditional TSN-DEA.

关键词： Biomass-biofuel logistics network two-stage network data envelopment analysis efficiency score decision-making unit multiple criteria

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Computed tomography coronary angiography after excluding myocardial infarction:high-sensitivity troponin versus risk score-guided approach

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World Journal of Emergency Medicine 2023年第6期14卷 428-433页

作者： Won jae yoo Shin Ahn Bora Chae Won Young Kim Department of Emergency Medicine Asan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505Republicof Korea

BACKGROUND:Patients with suspected acute coronary syndrome (ACS) in whom myocardial infarction has been ruled out are still at risk of having obstructive coronary artery disease (CAD).This rate is higher among patients with intermediate high-sensitivity troponin I (hsTnI) concentrations (5 ng/L to 99th percentile) than low concentrations (<5 ng/L).Therefore,an intermediate concentration has been suggested as a candidate for downstream investigation with computed tomography coronary angiography(CTCA).We tried to compare the HEART score-guided vs.hsTnI-guided approach for identifying obstructive CAD.METHODS:From a prospective cohort study of patients presenting to the emergency department with suspected ACS,433 patients without elevated hsTnI who also underwent CTCA were selected and analyzed.The performances of hsTnI concentration and HEART score were compared using sensitivity,specificity,positive predictive value (PPV),and negative predictive value (NPV).RESULTS:Overall,120 (27.7%) patients had obstructive CAD.Patients with intermediate hsTnI concentrations were more likely to have obstructive CAD than those with low hsTnI concentrations(40.0%vs.18.1%);patients with non-low-risk HEART scores (≥4 points) were also more likely to have obstructive CAD than those with low-risk scores (0 to 3 points)(41.0%vs.7.6%).The HEART score had higher sensitivity and NPV for detecting obstructive CAD in each classification than hsTnI concentration (sensitivity:89.2%vs.63.3%;NPV:92.4%vs.81.9%,respectively).CONCLUSION:After excluding myocardial infarction in patients with suspected ACS,adding the HEART score for selecting candidates for CTCA could improve patient risk stratification more accurately than relying on hsTnI concentration.

关键词： Chest pain Coronary artery disease Acute coronary syndrome Troponin HEART score

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