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Asian Pacific Journal of Tropical Biomedicine 2014年第2期4卷 97-103页

作者： Hye-Ryun Kim II-Doo Kim Sanjeev Kumar Dhungana Mi-Ok Kim dong-hyun shin Division of Plant Biosciences.School of Plant Biosciences Kyungpook National UniversityDaegu 702-701.Korea Institute of Agricultural Science and Technology Kyungpook national universityDaegu 702-701.Korea Department of Hotel Restaurant.Culinary ArtsDaegu Health CollageDaegu 702-722.Korea

Objective:To investigate the physicochemical properties of unripe peach-Pruuns persica ***(Mibaekdo) and Prunus persica *** Hakuho(Nagasawa Hakuho) as an alternative to fowl supplement while Japanese apricot(Prunus mume ***)(Backaha) was used as a control ***:The unripe fruits were analyzed for soluble solid(Brix).titratable ***,total polyphenol content,1,1-diphenyl-2-pierylhydrazyl(DPPH) radical scavenging activity,amygdalin content,free amino acid content,organic acid content,free sugar content,and α-amylase ***:Total polyphenol content of unripe peach ranged between 137.27-151.64 μg/g whereas that of apricot was 160.73 μg/*** radical scavenging activities of Rackaha was the highest189.16%.) followed by Mibaekdo(85.03%) and Nagasawa Hakuho(41.30%).The highest amount of oxalic acid 1612.8 mg/100 g) was observed in Mibaekdo while that of Nagasawa llakuho and Backaha were(184.6±18.1) and(334.8±16.1) mg/100 g,*** contents of *** Hakuho and Backaha were 486.61,548.60 and 174.28 μg/g,***:The results suggest that the unripe fruit of peach has a significant biochemical potential of using as a food supplement with potential health benefit for human health.

关键词： Amygdalin Japanese apricot(Backaha) Physicochemical properties Unripe peach(Mibaekdo Nagasawa Hakuho)

Hyperreflective photonic crystals created by shearing colloidal dispersions at ultrahigh volume fraction

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Microsystems & Nanoengineering 2024年第1期10卷 161-173页

作者： Minji Kim Jong Bin Kim shin-hyun Kim Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea

Colloidal crystallization serves as one of the most economic and scalable production methods for photonic ***,insufficient optical performance,nonuniformity and low reproducibility remain challenges for advanced high-value *** this study,we optimally formulate a photocurable dispersion of silica particles and apply shear flow to unify the orientation of the colloidal crystals,ensuring high optical performance and *** silica particles experience strong repulsion at ultrahigh volume fractions of 50%but demonstrate low mobility,leading to polycrystalline *** shear flow to the dispersions allows the silica particles to rearrange into larger crystalline domains with a unidirectional orientation along the *** shear-induced structural change produces absolute reflectivity at the stopband as high as 90%and a high transparency of 90%at off-resonant wavelengths with minimal diffusive ***,the strong interparticle repulsion ensures a uniform volume fraction of particles throughout the dispersion,reducing deviations in the optical *** intricately micropattern the photocurable dispersions using ***,the photonic films and patterns can be stacked to form multiple layers,displaying mixed structural colors and multiple reflectance peaks without sacrificing *** superior photonic materials hold promise for various optical applications,including optical components and anticounterfeiting patches.

关键词： properties. dispersion reflectivity

HAUSTORIUM INDUCTION OF PARASITIC PLANTS AS A NEW BIOASSAY METHOD

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HAUSTORIUM INDUCTION OF PARASITIC PLANTS AS A NEW BIOASSAY M...

PROGRESS AND PROSPECT OF RICE ALLOLOPATHY RESEARCH

中国第三届植物化感作用学术研讨会、第八届全国杂草科学大会、联合国粮农组织——中国“水稻化感作用论坛”

作者： Kil-Ung KIM dong-hyun shin Dept.of Agronomy College of Agriculture and Life SciencesKyungpook National UniversityDaegu(Taegu)Korea(South)

*** sterilization and germination *** seeds were surface sterilized in 70%ethanol for 15 min,2%NaClO added 1 drop of Tween 20 for 25 min and then rinsed in four to six volumes of sterile water. Approximately 200 seeds were placed in round Petri dishes(90 mm×25 mm) containing 0.1% Noble *** plates were sealed with parafilm,placed at 4℃for 2 days to stimulate germination,and then transferred to a 16℃growth incubator under a light condition. Germination occurred 10-14 days after sowing.

关键词： HAUSTORIUM INDUCTION OF PARASITIC PLANTS AS A NEW BIOASSAY METHOD ROS

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PROGRESS AND PROSPECT OF RICE ALLOLOPATHY RESEARCH

Bone-targeted lipoplex-loaded three-dimensional bioprinting bilayer scaffold enhanced bone regeneration

中国第三届植物化感作用学术研讨会、第八届全国杂草科学大会、联合国粮农组织——中国“水稻化感作用论坛”

作者： Kil-Ung KIM dong-hyun shin Department of Agronomy College of Agriculture and Life SciencesKyungpook National UniversityDaegu(Taegu) 702-701Korea(South)

An improvement in allelopathic potential in rice will have a great impact on both low-input and high-input management *** alone is not likely to replace other weed control practices,but it surely functions as a component of integrated weed management *** reduced use of herbicide over time will provide significant economical benefits to farmers and will also reduce adverse effects on the environment.

关键词： PROGRESS AND PROSPECT OF RICE ALLOLOPATHY RESEARCH

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Regenerative Biomaterials 2024年第4期3卷 138-148页

作者： Woo-Jin Kim Jeong-hyun Ryu Ji Won Kim Ki-Tae Kim Hye-Rim shin Heein Yoon hyun-Mo Ryoo Young-Dan Cho Department of Molecular Genetics School of Dentistry and Dental Research InstituteSeoul National UniversitySeoul03080Republic of Korea Department of Periodontology School of Dentistry and Dental Research InstituteSeoul National University and Seoul National University Dental HospitalSeoul03080Republic of Korea

Clinical bone-morphogenetic protein 2(BMP2)treatment for bone regeneration,often resulting in complications like soft tissue inflammation and ectopic ossification due to high dosages and non-specific delivery systems,necessitates research into improved biomaterials for better BMP2 stability and *** tackle this challenge,we introduced a groundbreaking bone-targeted,lipoplex-loaded,three-dimensional bioprinted bilayer scaffold,termed the polycaprolactone-bioink-nanoparticle(PBN)scaffold,aimed at boosting bone *** encapsulated BMP2 within the fibroin nanoparticle based lipoplex(Fibroplex)and functionalized it with DSS6 for bone tissue-specific targeting.3D printing technology enables customized,porous PCL scaffolds for bone healing and soft tissue growth,with a two-step bioprinting process creating a cellular lattice structure and a bioink grid using gelatin-alginate hydrogel and DSS6-Fibroplex,shown to support effective nutrient exchange and cell growth at specific pore *** PBN scaffold is predicted through in silico analysis to exhibit biased BMP2 release between bone and soft tissue,a finding validated by in vitro osteogenic differentiation *** PBN scaffold was evaluated for critical calvarial defects,focusing on sustained BMP2 delivery,prevention of soft tissue cell infiltration and controlled fiber membrane pore size in *** PBN scaffold demonstrated a more than eight times longer BMP2 release time than that of the collagen sponge,promoting osteogenic differentiation and bone regeneration in a calvarial defect *** findings suggest that the PBN scaffold enhanced the local concentration of BMP2 in bone defects through sustained release and improved the spatial arrangement of bone formation,thereby reducing the risk of heterotopic ossification.

关键词： 3D bioprinting bone-targeting lipoplex BMP2 bone regeneration

M1-polarized macrophage-derived cellular nanovesicle-coated lipid nanoparticles for enhanced cancer treatment through hybridization of gene therapy and cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第7期14卷 3169-3183页

作者： Ha Eun shin Jun-Hyeok Han Seungyong shin Ga-hyun Bae Boram Son Tae-hyung Kim Hee Ho Park Chun Gwon Park Wooram Park Department of Integrative Biotechnology Sungkyunkwan University(SKKU)SuwonGyeonggi 16419Republic of Korea Deparment of Inteligent Precision Healthcare Convergence SKKUSuwonGyeonggi 16419Republic of Korea Department of MetaBioHealth SKKU Institute for ConvergenceSKKUSuwonGyeonggi 16419Republic of Korea Department of Bioengineering Hanyang UniversitySeoul 04763Republic of Korea Department of Integrative Engineering Chung-Ang UniversitySeoul 06974Republic of Korea Department of Biomedical Engineering SKKUSuwonGyeonggi 16419Republic of Korea Korea Institute of Science and Technology(KIST) Seoul 02792Republic of Korea

Optimum genetic delivery for modulating target genes to diseased tissue is a major obstacle for profitable gene *** nanoparticles(LNPs),considered a prospective vehicle for nucleic acid delivery,have demonstrated efficacy in human use during the COVID-19 *** study introduces a novel biomaterial-based platform,M1-polarized macrophage-derived cellular nanovesicle-coated LNPs(M1-C-LNPs),specifically engineered for a combined gene-immunotherapy approach against solid *** dual-function system of M1-C-LNPs encapsulates Bcl2-targeting siRNA within LNPs and immune-modulating cytokines within M1 macrophage-derived cellular nanovesicles(M1-NVs),effectively facilitating apoptosis in cancer cells without impacting T and NK cells,which activate the intratumoral immune response to promote granule-mediating killing for solid tumor *** retention within tumor was observed upon intratumoral administration of M1-C-LNPs,owing to the presence of adhesion molecules on M1-NVs,thereby contributing to superior tumor growth *** findings represent a promising strategy for the development of targeted and effective nanoparticle-based cancer genetic-immunotherapy,with significant implications for advancing biomaterial use in cancer therapeutics.

关键词： Genetic-immunotherapy M1 macrophage-derived cellular nanovesicles Lipid nanoparticles(LNPs) Gene therapy siRNA Cancer immunotherapy Solid tumor Tumor microenvironment(TME)

Endoscopic radial incision and cutting method for adult congenital duodenal webs:A case report

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World Journal of Clinical Cases 2024年第18期12卷 3622-3628页

作者： hyun Deok shin Department of Internal Medicine Dankook University HospitalDankook University College of MedicineCheonan 31116ChungnamSouth Korea

BACKGROUND Congenital duodenal webs are rare in adults and can lead to various symptoms such as nausea,vomiting,and postprandial *** treatment for this disease is mostly *** treatment techniques have been developed and attempted for this *** radial incision and cutting(RIC)techniques are reportedly very effective in benign anastomotic *** case report highlights the effectiveness and safety of endoscopic RIC as a minimally invasive treatment for adult congenital duodenal *** SUMMARY A 23-year-old female patient with indigestion was referred to a tertiary *** patient complained of postprandial fullness in the epigastric *** physical examinations or blood tests indicated no *** tomography revealed an eccentric broad-based delayed-enhancing mass-like lesion in the second portion of the *** showed an enlarged gastric cavity and a significantly dilated duodenal bulb;a very small hole was observed in the distal part of the second portion,and scope passage was not *** upper gastrointestinal series was performed,revealing an intraduodenal barium contrast-filled sac with a curvilinear narrow radiolucent rim,a typical"windsock"*** RIC was performed on the duodenal *** patient recovered ***-up endoscopy showed a patent duodenal lumen without any residual *** patient reported complete resolution of symptoms at the 18-month *** Endoscopic RIC may be an effective treatment for congenital duodenal webs in adults.

关键词： Congenital duodenal web Endoscopic treatment Radial incision and cutting method Surgery Case report

Capsosiphon fulvescens suppresses LPS-stimulated inflammatory responses by suppressing TLR4/NF-κB activation in RAW264.7 murine macrophages

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Asian Pacific Journal of Tropical Biomedicine 2024年第3期14卷 115-126页

作者： Seon Yeong Ji EunJin Bang hyun Hwangbo Min Yeong Kim Da Hye Kim Su hyun Hong shin-hyung Park Chang-Young Kwon Gi-Young Kim You-Jin Jeon Suengmok Cho Yung hyun Choi Department of Biochemistry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Basic Research Laboratory for the Regulation of Microplastic-Mediated Diseases and Anti-Aging Research Center Dong-eui UniversityBusan 47227Republic of Korea Department of Pathology Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Oriental Neuropsychiatry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Marine Life Science Jeju National UniversityJeju 63243Republic of Korea Department of Food Science and Technology/Institute of Food Science Pukyong National UniversityBusan 48513Republic of Korea

Objective:To evaluate the effects of Capsosiphon fulvescens(***)ethanolic extract on inflammation in lipopolysaccharide(LPS)-induced RAW296.7 ***:The protective effects of *** ethanolic extract on LPS-induced inflammation in RAW264.7 macrophages were assessed using biochemical analysis,including enzyme-linked immunosorbent assay,quantitative reverse transcription-polymerase chain reaction,and Western blot *** examine reactive oxygen species(ROS)production,flow cytometry analysis,and immunofluorescence staining were ***,the modulatory effect of *** ethanolic extract on NF-κB activation was ***:*** ethanolic extract significantly attenuated LPS-induced levels of pro-inflammatory cytokines and notably reduced the secretion and mRNA levels of LPS-mediated matrix *** addition,*** ethanolic extract decreased ROS production and suppressed the TLR4/NF-κB signaling ***:*** ethanolic extract alleviates inflammation as well as oxidative stress by modulating the TLR4/NF-κB signaling in LPS-induced RAW264.7 *** can be used as a potential therapeutic agent to suppress inflammation and oxidative stress-associated diseases.

关键词： Capsosiphon fulvescens Inflammation Oxidative stress NF-κB Nrf2 TLR4

Understanding the correlation between energy-state mismatching and open-circuit voltage loss in bulk heterojunction solar cells

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Carbon Energy 2024年第5期6卷 164-174页

作者： hyun-Seock Yang Danbi Kim Chang-Mok Oh Vellaiappillai Tamilavan Pesi MHangoma Hojun Yi Bo RLee Insoo shin In-Wook Hwang Sung Heum Park Pukyong National University BusanRepublic of Korea Clarendon Laboratory University of OxfordOxfordUK Institute of Energy Transport and Fusion Research Pukyong National UniversityBusanRepublic of Korea Advanced Photonics Research Institute Gwangju Institute of Science and TechnologyGwangjuRepublic of Korea Department of Chemical and Biomolecular Engineering Tandon School of EngineeringNew York UniversityBrooklynNew YorkUSA

Photoinduced intermolecular charge transfer(PICT)determines the voltage loss in bulk heterojunction(BHJ)organic photovoltaics(OPVs),and this voltage loss can be minimized by inducing efficient PICT,which requires energy-state matching between the donor and acceptor at the BHJ ***,both geometrically and energetically accessible delocalized state matching at the hot energy level is crucial for achieving efficient *** this study,an effective method for quantifying the hot state matching of OPVs was *** degree of energy-state matching between the electron donor and acceptor at BHJ interfaces was quantified using a mismatching factor(MF)calculated from the modified optical density of the ***,the correlation between the open-circuit voltage(Voc)of the OPV device and energy-state matching at the BHJ interface was investigated using the calculated *** OPVs with small absolute MF values exhibited high Voc *** result clearly indicates that the energy-state matching between the donor and acceptor is crucial for achieving a high Voc in *** the MF indicates the degree of energy-state matching,which is a critical factor for suppressing energy loss,it can be used to estimate the Voc loss in OPVs.

关键词： bulk heterojunction open circuit voltage organic photovoltaics photoinduced charge transfer voltage loss

The CUL3A–LFH1–UBC15 ubiquitin ligase complex mediates SHORT VEGETATIVE PHASE degradation to accelerate flowering at high ambient temperature

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Plant Communications 2024年第4期5卷 224-238页

作者： Suhyun Jin Geummin Youn Sun Young Kim Taewook Kang hyun-young shin Ji-Yul Jung Pil Joon Seo Ji Hoon Ahn Department of Life Sciences Korea UniversitySeoul 02841Republic of Korea Department of Chemistry Seoul National UniversitySeoul 08826Republic of Korea These authors contributed equally to this article.

Ambient temperature affects flowering time in plants,and the MADS-box transcription factor SHORT VEGETATIVE PHASE(SVP)plays a crucial role in the response to changes in ambient *** protein stability is regulated by the 26S proteasome pathway and decreases at high ambient temperature,but the details of SVP degradation are ***,we show that SVP degradation at high ambient temperature is mediated by the CULLIN3–RING E3 ubiquitin ligase(CRL3)complex in Arabidopsis *** identified a previously uncharacterized protein that interacts with SVP at high ambient temperature and contains a BTB/POZ *** named this protein LATE FLOWERING AT HIGH TEMPERATURE 1(LFH1).Single mutants of LFH1 or CULLIN3A(CUL3A)showed late flowering specifically at 27***1 protein levels increased at high ambient *** found that LFH1 interacts with CUL3A in the cytoplasm and is important for SVP–CUL3A complex *** in CUL3A and/or LFH1 led to increased SVP protein stability at high ambient temperature,suggesting that the CUL3–LFH1 complex functions in SVP *** E2 ubiquitin-conjugating enzymes(UBCs)using RING-BOX PROTEIN 1(RBX1),a component of the CRL3 complex,as bait identified ***15 mutants also showed late flowering at high ambient *** vitro and in vivo ubiquitination assays using recombinant CUL3A,LFH1,RBX1,and UBC15 showed that SVP is highly ubiquitinated in an ATP-dependent ***,these results indicate that the degradation of SVP at high ambient temperature is mediated by a CRL3 complex comprising CUL3A,LFH1,and UBC15.

关键词： temperature-responsiveflowering SVP CUL3 proteasomal degradation ubiquitination