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Modeling the intracellular dynamics for Vif-APO mediated HIV-1 virus infection

chinese science Bulletin 2010年第22期55卷 2329-2340页

作者： wang Yi1,2 & LAI LuHua1,3.1 center for Theoretical Biology, Peking University, Beijing 100871, china 2 School of Physics, Peking University, Beijing 100871, china 3.BNLMS, State Key Laboratory for Structural chemistry of Unstable and Stable Species, College of chemistry and molecular Engineering, Peking University, Beijing 100871, china center for Theoretical Biology Peking University Beijing 100871 China School of Physics Peking University Beijing 100871 China BNLMS State Key Laboratory for Structural Chemistry of Unstable and Stable Species College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

The viral infectivity factor (Vif) was found to be essential for controlling HIV-1 virus infectivity. It targets cellular antiviral proteins in APOBEC family (APO) to trigger its fast degradation and inhibits APO packaging into nascent virion. In the present study, we propose a mathematical model to quantitatively study the intracellular dynamics of these typical virus-host interactions. Four sets of published experimental data were compared with simulation results to justify the model. Systematic parameter sensitivity and perturbation analysis showed that parameters related to APO are crucial to the infectivity of newly synthesized HIV-1 virus. Interestingly, we discovered that the synthesis rate of the viral structure protein Gag and the required number per nascent virion are optimized to achieve high virion production with minimal level of packaged APO, and large portion of model parameters are beneficial to virus only within a relatively small range. Furthermore, minor variations in several parameters related to viral protein Tat, the activator of viral RNA synthesis, were found to induce switch-like behaviors on both incorporated Vif and APO. These findings may provide new insights for understanding the high mutation rate of HIV-1 virus and its latency, as well as help identify key targets for therapeutic design.

关键词：病毒感染载脂蛋白 HIV 细胞内动力学抗病毒蛋白建模介导

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Progress in phototaxis mechanism research and micromanipulatlon techniques of algae cells

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Progress in Natural science:Materials International 2007年第3期 254-260页

作者： WEN Chenglu, LI Heng, wang Pengbo, LI Wei and ZHAO Jingquan (. College of Engineering, china Agricultural University, Beijing 100083. china Key Laboratory of Photo{3., center for molecular science, institute of {3., chinese academy of sciences, Beijing 100080, china)

Pholotaclic movement is a characteristic of some microorganisms' response to light environment. Most of the algae have dramatically phototactic responses, underlying the complicated biological, physical and photochemical mechanisms are involved. With the development of the micro/nano and sensor techniques, great progress has been made in the research of the algae phototaxis. This review article summarizes the progress made in the research on the functional phototactic structures, the mechanisms of photo-response process and photodynamics of phototaxis in algae, and describes the latest developed micro-tracking technique and micromanipulation technique. Moreover, based on our own research results, the potential correlation between the phototaxis and photosynthesis is discussed, and the directions for future research of the phototactic mechanism are proposed.

关键词： phototaxis algae photosynthesis tracking micromanipulation.

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Bacterial Type Ⅰ Glutamine Synthetase of the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U3.,is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions

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Acta Biochimica et Biophysica Sinica 2006年第12期 821-830页

作者： Wen-Tao PENG~(1&) Jin wang~1 Ting WU~2 Jian-Qiang HUANG~1 Jui-Shen CHIAO~1 Guo-Ping ZHAO~(1,3.4) 1 Laboratory of molecular Microbiology,institute of Plant Physiology and Ecology,Shanghai institutes for Biological sciences,chinese academy of sciences,Shanghai 20003.,china 2 Department of Medicinal chemistry,School of Pharmacy,Fudan University,Shanghai 20003.,china 3.State Key Laboratory of Genetic Engineering/Department of Microbiology,School of Life science,Fudan University,Shanghai 20043.,china 4 Laboratory of Health and Disease Genomics,chinese National Haman Genome center at Shanghai,Shanghai 201203.china

The structural gene for glutamine synthetase,glnA,from Amycolatopsis mediterranei U3. wascloned via screening a genomic library using the analog gene from Streptomyces *** clone wasfunctionally verified by complementing for glutamine requirement of an Escherichia coli glnA null mutantunder the control of a lac *** analysis showed an open reading frame encoding a protein of466 amino acid *** deduced amino acid sequence bears significant homologies to other bacterialtype Ⅰ glutamine synthetases,specifically,71% and 72% identical to the enzymes of *** and Myco-bacterium tuberculosis,*** of this glnA gene in *** U3. led to glutamineauxotrophy with no detectable glutamine synthetase activity in *** contrast,the cloned glnA+gene cancomplement for both phenotypes in *** thus suggested that in *** U3.,the glnA geneencoding glutamine synthetase is uniquely responsible for in vivo glutamine synthesis under our laboratorydefined physiological conditions.

关键词： glnA glutamine synthetase Amycolatopsis mediterranei rifamycin SV

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molecular Chain Flexibility and Dielectric Loss at High-Frequency:Impact of Ester Bond Arrangement in Poly(ester imide)s

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chinese Journal of Polymer science 2024年第8期42卷 1122-1133页

作者： Ya-dong Li Hong Li Lu-Kun Feng Feng Bao Ming-Liang wang Cai-Zhen Zhu Zhao-Hui Zheng Xiao-Bin Ding Jian Xu Chengdu institute of Organic chemistry Chinese Academy of SciencesChengdu 610041China University of chinese academy of sciences Beijing 100081China institute of Low-Dimensional Materials Genome Initiative College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen 518060China

The evolution of high-frequency communication has accentuated the significance of controlling dielectric properties in polymer ***,it has been theorized that rigid molecular chains lead to lower dielectric ***,the validity of this proposition at high frequencies remains *** scrutinize the correlation between chain flexibility and dielectric properties,we synthesized six poly(ester imide)s(PEIs)with systematically varied molecular chain flexibilities by modifying the ester's substitution on the aromatic *** introduction of ester bonds bestowed all PEI films with a low dielectric dissipation factor(D_(f)),ranging from 0.0021 to 0.003. at 10 GHz in dry *** dry D_(f)displayed a pattern consistent with volume polarizability(P/V).Unexpectedly,PI-mmm-T,featu ring the most flexible molecular chain,exhibited the lowest dielectric loss under both dry(0.0021@10 GHz)and hygroscopic(0.0029@10 GHz)***,the observed increase in D_(f)after humidity absorption suggests that the high dielectric loss of PEI in applications may be attributed to its hygroscopic *** simulations and characterization of the aggregation structure revealed that the smaller cavities within flexible molecular chains,after close stacking,impede the entry of water *** sacrificing high-temperature resistance,the precursor exhibited enhanced solubility properties and could be processed into high-quality *** research unveils new insights into the relationship between flexibility and highfrequency dielectric loss,offering innovative perspectives on synthesizing aromatic polymers with exceptional dielectric properties.

关键词： 5G communications Poly(ester imide)s molecular chain flexibility Dielectric properties Humidity absorption

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Individual dynamics and local heterogeneity provide a microscopic view of the epidemic spreading

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chinese Physics B 2024年第5期33卷 656-663页

作者：朱友源沈瑞哲董昊王炜 Kuang Yaming Honors School Nanjing UniversityNanjing 210023China Collaborative Innovation center of Advanced Microstructures National Laboratory of Solid State MicrostructureSchool of PhysicsNanjing UniversityNanjing 210093China institute for Brain sciences Nanjing UniversityNanjing 210023China State Key Laboratory of Analytical chemistry for Life science Nanjing UniversityNanjing 210023China

The COVID-19 pandemic has caused severe global disasters,highlighting the importance of understanding the details and trends of epidemic transmission in order to introduce efficient intervention *** the widely used deterministic compartmental models have qualitatively presented continuous “analytical” insight and captured some transmission features,their treatment usually lacks spatiotemporal ***,we propose a stochastic individual dynamical(SID)model to mimic the random and heterogeneous nature of epidemic *** SID model provides a unifying framework for representing the spatiotemporal variations of epidemic development by tracking the movements of each *** this model,we reproduce the infection curves for COVID-19 cases in different areas globally and find the local dynamics and heterogeneity at the individual level that affect the disease *** macroscopic trend of virus spreading is clearly illustrated from the microscopic perspective,enabling a quantitative assessment of different ***,this model is also applicable to studying stochastic processes at the “meter scale”,e.g.,human society’s collective dynamics.

关键词： Brownian motion epidemic spreading heterogeneity

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Azocalixarenes: a scaffold of universal excipients with high efficiency

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science china chemistry 2024年第5期67卷 1697-1706页

作者： Yu-Xin Yue Yi-Lin Lin Meng-Meng Chen Han-Wen Tian Rong Ma Ze-Han wang Fang-Yuan Chen Yu-Chen Pan dong-Sheng Guo College of chemistry State Key Laboratory of Elemento-Organic ChemistryKey Laboratory of Functional Polymer Materials(Ministry of Education)Frontiers Science Center for New Organic MatterCollaborative Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China

Excipients are important components of pharmaceutical preparations that affect their quality, safety, and efficacy. Macrocyclic receptors are a family of supramolecular excipients with several advantages, including molecular-level protection, small sizes,fast kinetics of host-guest recognition, and modular construction. With the continuous advances in the medical field, personalized and precision medicine requires the development of excipients with low dosages, integrated modifying effects, universality,and controlled release. To meet these requirements, we have developed a new family of macrocyclic excipients based on calixarenes by integrating their covalent(broad chemical design space) and noncovalent(wide range of substrates) ***, azocalixarenes(Azo CAs) were designed, showing high binding affinities to a broad spectrum of active pharmaceutical ingredients(APIs), selectivity to interferents, and responsiveness to hypoxic microenvironments. Due to their highly efficient and controllable recognition, Azo CAs serve as low-dose excipients for 3. APIs. molecular encapsulation by Azo CAs results in the integrated modification of the physicochemical properties of APIs, including solubility, stability, bioavailability,and biocompatibility. Moreover, Azo CAs can be reduced by azoreductases overexpressed in hypoxic microenvironments,leading to the controlled release of APIs. Collectively, Azo CA excipients have broad application prospects for a series of diseases such as enteritis, arthritis, stroke, cancer, bacterial infection and kidney injury, with diverse therapeutic modalities,including chemotherapy, photodynamic therapy, photothermal therapy, immunotherapy, boron neutron capture therapy, radiotherapy, fluorescence imaging, and their combinations.

关键词： supramolecular chemistry host-guest systems excipient calixarene molecular recognition

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Synthesis and characterization of a three-ring bent-core compound

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science Bulletin 2005年第17期 35-39页

作者： TANG Yanhong1, MEI Chongyu1, LI Shaojun2, wang Yan1, wang Xingdong1, wang Lixiang1, YAN donghang1 & YE Cheng2 1. State Key Laboratory of Polymer Physics and chemistry, Changchun institute of Applied chemistry, chinese academy of sciences, Changchun 13.022, china 2. center for {13. science, Laboratory of Organic Solid, institute of chemistry, chinese academy of sciences, Beijing 100080, china

To investigate the vacuum-deposited films of the banana-shaped mesogens, we prepared a three-ring bent-core (that is, banana-shaped) compound m-bis(4-p- octoxysryrenyl)benzene (m-OSB) and used it as a prototype for studying the related film physics. Herein the synthesis and the structural characterization of m-OSB are reported. 13. and 1H NMR spectra and IR spectrum confirm that m-OSB has a symmetric and all-trans conformation. The results of differential scanning calorimetry, polarizing optical microscopy and X-ray diffractometry indicate that no liquid crystalline phase but two crystalline phases are present in this material: phase I (T<66℃) and phase II (66℃更多还原AbstractFilter('ChDivSummary', 'ChDivSummaryMore', 'ChDivSummaryReset');

关键词： banana-shaped mesogens bent-core molecules anti-ferro-electric alignment.

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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

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chinese science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2,3.4, wang Kemin1,2,3.4, XIAO Zhiqiang5,6, wang Wei1,3.4, TAN Weihong1,2,3.4, SUN Yi5,6, TANG Hongxing1,3.4 & YANG Xiaohai1,3.41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan University, Changsha 410082, china 2. Life {3. and Biotechnology institute, Hunan University, Chang- sha 410082, china 3. chemistry and Chemical Engineer College, Hunan University, Changsha 410082, china 4. Hunan Engineering Research center for Bio-Nanotechnology, Hunan University, Changsha 410082, china 5. Medical Experiment Research center, Xiangya Hospital, Central South University, Changsha 410008, china 6. Key Laboratory of Cancer Proteomics of chinese Ministry of Health, Changsha 410008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China Life science and Biotechnology institute Hunan University Changsha China chemistry and Chemical Engineer College Hunan University Changsha China Hunan Engineering Research center for Bio-Nanotechnology Hunan University Changsha China Medical Experiment Research center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53.RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53.with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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Studies on the syntheses and properties of Schiff base type crown ethers

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chinese Journal of chemistry 1993年第4期11卷 360-365页

作者： W.U,Yang-Jie ZHAO,Bin ZHAO,Li-wang Department of chemistry,Zhengzhou University,Zhengzhou,Henan 450052SHEN,Lian-Fang WU,dong-Hui Laboratory of Magnetic Resonance and Atomic and {3. Physics,Wuhan institute of Physics,chinese academy of sciences,Wuhan,Hubei 43.071 1Department of chemistry Zhengzhou University Zhengehou Eenan 4500522Laboratory of Magnetic Resonance and Atomic and Molecular Physios Wuhan Institute of Physics Chinese Academy of Sciences Wuhan Hubei 430071

Eight bis-,two tris-and two mono-crown ethers of Schiff base type have been pre- parcd by the reaction of 4'-formylbenzo-15-crown-5 with *** measurements show that the bis-crown ethers form 1:1 complexes(crown cther u... 详细信息

关键词： Studies on the syntheses and properties of Schiff base type crown ethers type base

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Unconventional Organic Low-Dimensional Crystals:Diversity and Potential Applications

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CCS chemistry 2024年第7期6卷 1628-1653页

作者： Jin Feng Mao Sun Ying-Xin Ma Zhen-Yu Geng Chuan-Zeng wang Shu-Ping Zhuo Shu-Hai Chen Hong-Tao Lin Xue-dong wang School of chemistry and Chemical Engineering Shandong University of TechnologyZibo 255000 School of Resources and Environmental Engineering Shandong University of TechnologyZibo 255000 institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123

Organic low-dimensional crystals have garnered escalating interests in the realms of miniaturized optoelectronics and integrated *** continual expansion of molecular systems and improvement in experimental conditions have given rise to unconventional organic low-dimensional crystals,which showcase a variety of appealing structure-dependent properties in the realm of organic semiconductors,owing to their adaptable physicochemical characteristics and exceptional optoelectronics ***,the development of unconventional low-dimensional crystals is filled with abundant possibilities due to their diverse application ***,we present a comprehensive overview of advancements in research on representative cases of unconventional low-dimensional organic crystals,using a systematic and rational structural ***,we have also discussed the existing challenges and future directions,aspiring to deepen understanding and encourage further research exploration in this field.

关键词： organic semiconductor crystal growth precise synthesis noncovalent interaction integrat-ed photonics

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