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Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1△*** genes

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Chinese Medical Journal 2011年第17期124卷 2642-2648页

作者： XUE Shou-ru CHEN Chong-fang dong wan-li HUI Guo-zhen LIU Tian-jun GUO Li-he Department of Neurology First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 China Department of Neurosurgery First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 China Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Background Human amniotic epithelial cells （HAECs）, which have characteristics of both embryonic and pluripotent stem cells, are therefore a candidate in cell therapy without creating legal or ethical problems. In the present study, we aimed to investigate the effects of intracerebroventricular transplantation of HAECs on doubly transgenic mice of Alzheimer＇s disease （AD） coexpressing presenilin-1 （PS1） and mutant Sweden amyloid precursor protein （APPswe） genes. Methods The offspring mice genotypes were detected using PCR identification of APPswe and PS1 gene. The doubly transgenic （TG） mice （n=20） and wild-type （WT） mice （n=20） were randomly divided into two groups respectively： the transplantation group treated with HAECs and the control group with phosphate buffered saline. Six radial arm water maze test was used to assess the spatial memory in the TG and WT mice. Amyloid plaques and neurofibrillary tangles were analyzed using congo red and acid-silver methenamine staining respectively. was used to track the survival of HAECs. Immunohistochemistry was used octamer-binding protein 4 （Oct-4） and Nanog in the HAECs. High performance measure acetylcholine in hippocampus. The density of cholinergic neurons in hippocampus was measured using acetylcholinesterase staining. Immunofluorescence cytochemistry to determine the expression of quid chromatography was used to basal forebrain and nerve fibers in Results Amyloid deposition occurred in hippocampus and frontal cortex in the double TG mice aged 8 months, but not in WT mice. The results also showed that transplanted HAECs can survive for at least 8 weeks and migrate to the third ventricle without immune rejection. The graft HAECs can also express the specific marker Oct-4 and Nanog of stem cell. Compared with the control group, transplantation of HAECs can not only significantly improve the spatial memory of the TG mice, but also increase acetylcholine concentration and the number of hippocampal cholinergic neurites.

关键词： Alzheimer's disease human amniotic epithelial cells transgenic mice spatial memory deficit

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Effect of dopant valence on the oxygen desorption and oxygen permeability of SrCo_(0.4)Fe_(0.5)M_(0.1)O_(3-δ)(M=Ni, Al and Zr) mixed-conducting oxides

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Chinese Chemical Letters 2008年第6期19卷 725-729页

作者： Yan Jun He Xian Feng Chang Xue Liang dong Chun Zhang wan Qin Jin Nan Ping Xu State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemistry and Chemical Engineering Nanjing University of Technology Nanjing 210009 China

The effect of dopant valence on oxygen desorption and oxygen permeability of SrCo0.4Fe0.5M0.1O3-δ （M = Ni, Al and Zr） mixed-conducting oxides were investigated in detail by O2-TPD and oxygen permeation measurement. The SrCo0.4Fe0.5M0.1O3-δ for M = Fe, Ni, Al and Zr were denoted as SCF, SCFN, SCFA and SCFZ, respectively. O2-TPD analysis revealed that the amount of α oxygen desorption decreased with increasing the valance of doped metal elements （SCFN 〉 SCFA SCF 〉 SCFZ）. The oxygen permeation flux at the temperature ≈igher than 1148 K decreased in the order of SCFN 〉 SCF 〉 SCFZ 〉 SCFA. Single activation for oxygen permeation was observed for SCFZ oxide and the activation energies of SCF and SCFA change at around 1073 K, while the change temoerature of SCFN was about 1173 K.

关键词： Valence Mixed-conducting oxide Oxygen desorption Oxygen permeability

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Study of Spectral Character of Alkali Metals Using Microwave Plasma Torch Simultaneous Spectrometer

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Chemical Research in Chinese Universities 2006年第6期22卷 703-707页

作者： FENG Guo-dong wan Yi HUAN Yan-fu JIANG jie LI Ming CAO Yan-bo YU Ai-min JIN Qin-han Institute of Miniature Analytical Instrumentation College of Chemistry Jilin University Jilin Province Research Center for Engineering and Technology of Spectral Instruments Changchun 130023 P. R. China

A microwave plasma torch （MPT） simultaneous spectrometer was used to study the spectral character and the matrix effect on alkali metal ions in solution. The main parameters were optimized. The microwave forward power was 100 W. The argon flow rate that was used to sustain the Ar-MPT included the flow rate of carrier gas and the flow rate of support gas, which were 0. 8 and 1.0 L/min, respectively. The HC1 concentration in the solution was 0.02 mol/L. The observation height was 9. 0 ram. The detection limits of Li, Na, K, Rb, and Cs were 0. 0003, 0. 0004, 0. 009, 0.07 and 2.4 mg/L, respectively, and the resuhs obtained by the Ar-MPT were compared with those obtained by argon inductively coupled plasma（Ar-ICP） and argon microwave induced plasma（Ar-MIP）. The interference effects of several matrix elements were also studied.

关键词： Alkali metals Microwave plasma torch Simultaneous spectrometer Matrix effects

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Plasma metabolites and risk of myocardial infarction:a bidirectional Mendelian randomization study

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Journal of Geriatric Cardiology 2024年第2期21卷 219-231页

作者： dong-Hua LI Qiang WU Jing-Sheng LAN Shuo CHEN You-Yi HUANG Lan-Jin WU Zhi-Qing QIN Ying HUANG wan-Zhong HUANG Ting ZENG Xin HAO Hua-Bin SU Qiang SU Department of Cardiovascular Medicine Minzu Hospital of Guangxi Zhuang Autonomous RegionGuangxiChina Senior Department of Cardiology the Sixth Medical CenterChinese PLA General HospitalBeijingChina Library of Graduate School Chinese PLA General HospitalBeijingChina Department of Cardiology Jiangbin Hospital of Guangxi Zhuang Autonomous RegionGuangxiChina Health Management Institute the Second Medical CenterChinese PLA General HospitalBeijingChina

BACKGROUND Myocardial infarction(MI)is a critical cardiovascular event with multifaceted etiology,involving several genetic and environmental *** is essential to understand the function of plasma metabolites in the development of MI and unravel its complex *** This study employed a bidirectional Mendelian randomization(MR)approach to investigate the causal relationships between plasma metabolites and MI *** used genetic instruments as proxies for plasma metabolites and MI and conducted MR analyses in both directions to assess the impact of metabolites on MI risk and vice *** addition,the large-scale genome-wide association studies datasets was used to identify genetic variants associated with plasma metabolite(1400 metabolites)and MI(20,917 individuals with MI and 440,906 individuals without MI)*** variance weighted was the primary method for estimating causal *** estimates are expressed as beta coefficients or odds ratio(OR)with 95%*** We identified 14 plasma metabolites associated with the occurrence of MI(P<0.05),among which 8 plasma metabolites[propionylglycine levels(OR=0.922,95%CI:0.881–0.965,P<0.001),gamma-glutamylglycine levels(OR=0.903,95%CI:0.861–0.948,P<0.001),hexadecanedioate(C16-DC)levels(OR=0.941,95%CI:0.911–0.973,P<0.001),pentose acid levels(OR=0.923,95%CI:0.877–0.972,P=0.002),X-24546 levels(OR=0.936,95%CI:0.902–0.971,P<0.001),glycine levels(OR=0.936,95%CI:0.909–0.964,P<0.001),glycine to serine ratio(OR=0.930,95%CI:0.888–0.974,P=0.002),and mannose to trans-4-hydroxyproline ratio(OR=0.912,95%CI:0.869–0.958,P<0.001)]were correlated with a decreased risk of MI,whereas the remaining 6 plasma metabolites[1-palmitoyl-2-arachidonoyl-GPE(16:0/20:4)levels(OR=1.051,95%CI:1.018–1.084,P=0.002),behenoyl dihydrosphingomyelin(d18:0/22:0)levels(OR=1.076,95%CI:1.027–1.128,P=0.002),1-stearoyl-2-docosahexaenoyl-GPE(18:0/22:6)levels(OR=1.067,95%CI:1.027–1.109,P=0.001),alpha-ketobutyrate levels(OR=1.108,95%

关键词： infarction alterations diagnosis

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Synthesis and Characterization of Biomimetic Fe3O4/Coke Magnetic Nanoparticles Composite Material

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Journal of Wuhan University of Technology(Materials Science) 2016年第2期31卷 254-259页

作者：卢露露李文兵 wanG Guanghua ZHANG Zheng wan dong Lü Lijun LIU Nianru CHEN Biao JIANG Shiyun College of Chemical Engineering and Technology Wuhan University of Science and Technology Wuhan 430081 China College of Biological and Chemicial Engineering Guangxi University of Science and Technology Guangxi 545006 China

A composite material（Fe3O4/Coke）using coke supported Fe3O4 magnetic nanoparticles was successfully prepared via an in-situ chemicaloxidation precipitation method and characterized by SEM,XRD,Raman,and *** results showed that the Fe3O4 nanoparticles existed steadily on the surface of coke,with better dispersing and smaller particle *** catalytic ability of Fe3O4/Coke were investigatied by degrading p-nitrophenol（P-NP）.The results showed that the apparent rate constant for the P-NP at 1.0 g·L^-1 catalyst,30 mmol·L^-1 H2O2,pH=3.0,30 ℃ and the best ratio of Coke/Fe3O4 0.6,was evaluated to be 0.027 min^-1,the removalrate of CODCr was 75.47%,and the dissolubility of Fe was 2.42 mg·L^-*** with pure Fe3O4,the catalytic ability of Fe3O4/Coke in the presence of H2O2 was greatly *** Fe3O4/Coke was a green and environmentalcatalyst with high catalytic activity,showing a good chemicalstability and reusability.

关键词： biomimetic Fe3O4/Coke heterogeneous catalyst P-NP

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Endoscopic ultrasound-guided choledochoduodenostomies with fully covered self-expandable metallic stents

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World Journal of Gastroenterology 2012年第32期18卷 4435-4440页

作者： Tae Jun Song Yil Sik Hyun Sang Soo Lee Do Hyun Park dong wan Seo Sung Koo Lee Myung-Hwan Kim Department of Internal Medicine Inje University Ilsan Paik HospitalKoyang 411-706South Korea Department of Internal Medicine University of Ulsan College of MedicineAsan Medical CenterSeoul 138-736South Korea

AIM:To investigate the long-term outcomes of endoscopic ultrasound-guided choledochoduodenostomy(EUS-CDS) with a fully covered self-expandable metallic stent(FCSEMS).METHODS:From April 2009 to August 2010,15 patients with distal malignant biliary obstructions who were candidates for alternative techniques for biliary decompression due to a failed endoscopic retrograde cholangiopancreatography(ERCP) were *** 15 patients consisted of 8 men and 7 women and had a median age of 61 years(range:30-91 years).The underlying causes of the distal malignant biliary obstruction were pancreatic cancer(n = 9),ampulla of Vater cancer(n = 2),renal cell carcinoma(n = 1),advanced gastric cancer(n = 1),lymphoma(n = 1),and duodenal cancer(n = 1).RESULTS:The technical success rate of EUS-CDS with an FCSEMS was 86.7%(13/15),and functional success was achieved in 100%(13/13) of those *** two patients,the EUS-CDS failed because an FCSEMS with a delivery device could not be passed into the common bile *** mean duration of stent patency was 264 *** adverse events developed in three patients(3/13,23.1%),including self-limited pneumoperitoneum in two patients and cholangitis requiring stent reposition in one *** the follow-up period(median:186 d,range:52-388 d),distal stent migration occurred in four patients(4/13,30.8%).In 3 patients,the FCSEMS could be reinserted through the existing choledochoduodenal fistula ***:EUS-CDS with an FCSEMS is technically feasible and can lead to effective palliation of distal malignant biliary obstructions after failed ERCP.

关键词：金属支架内镜引导超声覆膜胰腺癌完全覆盖肠吻合术

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生育政策、成本社会化与人口出生率

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贵州财经大学学报 2022年第2期 69-78页

作者：许坤胡东婉刘扬西南财经大学中国金融研究院四川成都610074 中央财经大学金融学院北京102206 西南财经大学金融学院四川成都610074

我国计划生育政策调整引起了社会的高度关注,科学评价政策实施的效果尤为重要。以2001年《人口与计划生育法》提倡一对夫妻符合法律、法规规定条件的,可生育两个子女作为准自然实验,基于1982~2013年省级年度面板数据,采用面板数据和门... 详细信息

我国计划生育政策调整引起了社会的高度关注,科学评价政策实施的效果尤为重要。以2001年《人口与计划生育法》提倡一对夫妻符合法律、法规规定条件的,可生育两个子女作为准自然实验,基于1982~2013年省级年度面板数据,采用面板数据和门槛模型评估生育政策实施的有效性及机制问题。实证研究结果表明:生育政策与人口出生率显著为正;生育政策和生育成本社会化的交互项与人口出生率显著为正。说明生育政策有效,可以提高人口出生率,且生育成本社会化转移越多,人口出生率越高。从地区分组来看,东、中部地区的政策有效性更强。从门槛效应看,相对于低房价地区,高房价地区的政策效果更明显;相对于低文化水平群体,高文化水平群体的政策效果更明显。

关键词： “二孩”政策成本社会化政策评价

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大采高综采工作面切眼扩帮成巷及支架安装一体化技术研究与应用

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煤炭与化工 2022年第3期45卷 20-23,26页

作者：王东冀中能源股份有限公司东庞矿北井河北邢台054000

本文阐述了采用机械化的方法对工作面开切眼进行扩帮的同时进行支架安装,东庞矿21221综采工作面安装采用边扩边安工艺,实现了工作面安装过程中扩帮与支架就位平行作业,减少了扩帮区域顶板支护工序,缩短了工作面安装时间,缩短综采工作面... 详细信息

本文阐述了采用机械化的方法对工作面开切眼进行扩帮的同时进行支架安装,东庞矿21221综采工作面安装采用边扩边安工艺,实现了工作面安装过程中扩帮与支架就位平行作业,减少了扩帮区域顶板支护工序,缩短了工作面安装时间,缩短综采工作面设备搬家时间、提高采区接续率和经济效益,确保了东庞矿安全高产高效生产。

关键词：扩安一体化平行作业扩老空帮

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双水平SAGD井组调控技术研究与应用

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承德石油高等专科学校学报 2022年第3期24卷 26-30页

作者：董婉中油辽河油田公司辽宁盘锦124010

辽河油田杜84块馆陶油藏为边、顶、底水巨厚块状超稠油油藏,为探索超稠油吞吐后期接替技术,2005年在该区域成功开展了直平组合SAGD先导试验,为进一步提高该区域开发效果,在油藏边部设计了一对双水平SAGD井组。通过精细地质研究,确定了... 详细信息

辽河油田杜84块馆陶油藏为边、顶、底水巨厚块状超稠油油藏,为探索超稠油吞吐后期接替技术,2005年在该区域成功开展了直平组合SAGD先导试验,为进一步提高该区域开发效果,在油藏边部设计了一对双水平SAGD井组。通过精细地质研究,确定了合理的注采井距、安全的边水距离;创新设计了循环预热参数界限,制定了转驱的标准,通过精细的调控,生产井水平段动用程度达到了75%以上,成功建立了地下温场与注采井的连通,转驱后通过合理的控制SUBCOOL值及注采压差,促进蒸汽腔均衡扩展,井组产量快速上升至100 t以上,培育了国内第一口双水平SAGD百吨井。

关键词：超稠油油藏 SAGD 双水平井,循环预热参数调控

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OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

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Molecular Plant 2024年第6期17卷 900-919页

作者： Kun dong Fuqing Wu Siqi Cheng Shuai Li Feng Zhang Xinxin Xing Xin Jin Sheng Luo Miao Feng Rong Miao Yanqi Chang Shuang Zhang Xiaoman You Peiran wang Xin Zhang Cailin Lei Yulong Ren Shanshan Zhu Xiuping Guo Chuanyin Wu dong-Lei Yang Qibing Lin Zhijun Cheng Jianmin wan State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China

Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains *** this study,we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-of-function mutants that exhibit various abnormal spikelet ***,we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and *** showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs,thereby promoting the ubiquitination of OsJAZ1 by the SCF^(OsCOI1a/OsCOI1b) complex and degradation via the 26S *** process ultimately releases OsMYC2,a core transcriptional regulator in the JA signaling pathway,to activate or repress JA-responsive genes,thereby maintaining normal plant(spikelet)***,in the osprmt6a-1 mutant,reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/OsCOI1b in the presence of *** a result,OsJAZ1 proteins become more stable,repressing JA responses,thus causing the formation of abnormal spikelet ***,we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner,thereby establishing a negative feedback loop to balance JA *** further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high ***,our study establishes a direct molecular link between arginine methylation and JA signaling in rice.

关键词： protein arginine methylation jasmonate signaling negative feedback loop spikelet development adaptation to heat stress rice

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