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Effects of serum of the rats ventilated with high tidal volume on endothelial cell permeability and therapeutic effects of ulinastatin

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Chinese Medical Journal 2006年第16期119卷 1374-1380页

作者： HUO Guo-dong CAI shao-xi chen Bo chen Ying-hua Department of Respiration Nanfang Hospital Southern Medical University Guangzhou 510515 China

Background With the widespread use of ventilators in treating critically ill patients, the morbidity of ventilator-induced lung injury （VILI） is increasing accordingly. VILI is characterized by a considerable increase in microvascular leakiness and activation of inflammatory processes. In this study we investigated the effects of inflammatory mediators in VILI rat serum on endothelial cytoskeleton and monolayer cellular permeability, as well as the therapeutic effect of ulinastatin, to explore the pathogenesis and the relationship between biotrauma and lung oedema induced by VILI. Methods Thirty healthy male Sprague-Dawley rats were randomly divided into three groups： group A （normal tidal volume ventilation）, group B （high tidal volume ventilation） and group C （high tidal volume ventilation plus ulinastatin）. The serum of each rat after ventilation was added to endothelial cell line ECV-304 medium for two hours to observe the effects of serum and/or ulinastatin on endothelial fibrous actin and permeability. Results Compared to rats ventilated with normal tidal volume, serum of rats ventilated with high tidal volume caused a striking reorganization of actin cytoskeleton with a weakening of fluorescent intensity at the peripheral filament bands and formation of the long and thick stress fibres in the centre resulting in endothelial contraction and higher permeability. Prior treatment with ulinastatin lessened the above changes significantly. The changes of permeability coefficient of endothelial permeability after group A, B or C rats serum stimulation were （6.95 ± 1.66）%, （27.50±7.77）% and （17.71±4.66）% respectively with statistically significant differences （P〈0.05） among the three groups. Conclusions The proinflammatory mediators in the serum of the rats given high tidal volume ventilation increases endothelial permeability by reorganizing actin cytoskeleton, and pretreatment with ulinastatin lessens the permeability by inhibiting of proinflammatory mediator

关键词： ventilation proinflammatory mediators cytoskeleton permeability ulinastatin

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Effect of JIANPI HUOXUE decoction on inflammatory cytokine secretion pathway in rat liver with lipopolysaccharide challenge

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World Journal of Gastroenterology 2008年第12期14卷 1851-1857页

作者： Jing-Hua Peng Yi-Yang Hu Yang cheng Chong Han Li-Li Xu Qin Feng shao-dong chen Qing Tao Hong-Shan Li Xue-Mei Li Institute of Liver Diseases Shuguang HospitalShanghai University of Traditional Chinese Medicine Shanghai201203 China

AIM： To evaluate the effect of Chinese traditional medicinal prescription, JIANPI HUOXUE decoction （JHD） on cytokine secretion pathway in rat liver induced by lipopolysaccharide （LPS）. METHODS： Twenty-four male SD rats were divided into normal group （n = 4）, model group （n = 10） and JHD group （n = 10） randomly. Rats in model group and JHD group were administrated with normal saline or JHD via gastrogavage respectively twice a day for 3 d. One hour after the last administration, rats were injected with LPS via tail vein, 50 μg/kg. Simultaneously, rats in normal group were injected with equivalent normal saline. After LPS stimulation for 1.5 h, serum and liver tissue were collected. Pathological change of liver tissues was observed through hematoxylineosin （H.E.） staining. Tumor necrosis factor alpha （TNF-α） in serum were assayed by enzyme linked immunosorbent assay （ELISA）. The protein expression of TNF-α, phosphorylated inhibit-κB （p-κB） and CD68 in liver were assayed by Western blot. The distribution of CD68 protein in liver was observed through immunohistochemical staining. The mRNA expression of TNF-α, interleukin-6 （IL-6）, CD14, toll-like receptor 2 （TLR2） and TLR4 in liver were assayed by real-time ***： Predominant microvesicular change, hepatocyte tumefaction and cytoplasm dilution were observed in liver tissues after LPS administration as well as obvious CD68 positive staining in hepatic sinusoidal. After LPS stimulation, serum TNF-α （31.35 ± 6.06 vs 12225.40 ± 9007.03, P 〈 0.05）, protein expression of CD68 （1.13 ± 0.49 vs 3.36 ±1.69, P 〈 0.05）, p-IκB （0.01 ±0.01 vs 2.07 ＋ 0.83, P 〈 0.01） and TNF-α （0.27 ± 0.13 vs 1.29 ± 0.37, P 〈 0.01） in liver and mRNA expression of TNF-α （1.96 ± 2.23 vs 21.45 ±6.00, P 〈 0.01）, IL-6 （4.80 ± 6.42 vs 193.50 ± 36.36, P 〈 0.01） and TLR2 （1.44 ± 0.62 vs 4.16 ± 0.08, P 〈 0.01） in liver were also increased significantly. These pathological changes were all improved in .1HD group. On the other hand, TLR4 mRNA （1.22 ± 0.3

关键词： JIANPI HUOXUE decoction Lipopolysaccharide Kupffer cell, Cytokine Endotoxin receptor

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Nutritional Support During Chemotherapy in a Patient with Advanced Gastric Cancer: A Case Report

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Journal of Nutritional Oncology 2018年第4期3卷 193-197页

作者： Xiao Wei Xin Yu Fang dong shao chen Xiao Tian Zhang Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Department of Clinical Nutrition Peking University Cancer Hospital and Institute Haidian District Beijing 100000 China Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Department of Gastrointestinal Oncology Peking University Cancer Hospital and Institute Haidian District Beijing 100000 China

Gastric cancer (GC) is one of the most common malignancies in China. GC patients often present with dysphagia and abnormal digestive function, leading to malnutrition or even cachexia. In February 2017, an advanced GC patient at Peking University Cancer Hospital received chemotherapy with the additional support of nutritional treatment. After being treated with the dietary prescription and oral nutritional supplements, the patient’s malnutrition was reversed, and he was able to complete five full cycles of chemotherapy.

关键词： Gastric cancer Malnutrition Patient-generated subjective global assessment Chemotherapy Nutritional treatment

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li dongqing Sun Shaliu Fu Lei Gu Xin shao Qinqin Wang Xin dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-dong J.Han Qi Liu Xiaohui Fan chen Li chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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投资者关注与过度投资及权益资本成本的中介效应

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商业研究 2017年第8期 90-98页

作者：汤晓冬陈少华厦门大学管理学院

本文从过度投资的视角分析投资者关注对企业资源配置效率的影响,并结合行为金融学的价格压力假说考察股权融资能力是否属于影响的渠道。研究发现:投资者关注与过度投资呈现正相关关系,其中权益资本成本是投资者关注对过度投资影响的中... 详细信息

本文从过度投资的视角分析投资者关注对企业资源配置效率的影响,并结合行为金融学的价格压力假说考察股权融资能力是否属于影响的渠道。研究发现:投资者关注与过度投资呈现正相关关系,其中权益资本成本是投资者关注对过度投资影响的中介变量;随着投资者关注程度的提高,管理层为了迎合投资者的需求,提高了投资水平,恶化了过度投资。在这个过程中,投资者关注有助于降低权益资本成本,进而向上市公司管理层传递了融资能力变化的信息,降低了潜在的股权融资约束,引起投资水平的提高,并导致投资效率的降低。

关键词：投资者关注过度投资权益资本成本中介效应

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非酒精性脂肪性肝病与肝细胞凋亡调控机制的研究进展

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世界华人消化杂志 2008年第12期16卷 1327-1331页

作者：陈少东胡义扬上海中医药大学附属曙光医院上海中医药大学肝病研究所

非酒精性脂肪性肝病的发病机制至今尚不十分清楚.近年来众多研究表明,本病存在肝细胞凋亡的异常,说明肝细胞凋亡与本病的发生密切相关.调控细胞凋亡的机制可分正向/负向两个方面的调控因素.本文就近几年在非酒精性脂肪性肝病肝细胞凋亡... 详细信息

非酒精性脂肪性肝病的发病机制至今尚不十分清楚.近年来众多研究表明,本病存在肝细胞凋亡的异常,说明肝细胞凋亡与本病的发生密切相关.调控细胞凋亡的机制可分正向/负向两个方面的调控因素.本文就近几年在非酒精性脂肪性肝病肝细胞凋亡调控机制方面的研究进展作一综述.

关键词：非酒精性脂肪性肝病细胞凋亡肝细胞

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Microstructural evolution during the progressive transformation-induced plasticity effect in a Fe-0.1C-5Mnmedium manganese steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 369-379页

作者： Mei Zhang Wenhao Li Yangfei chen Yang Jiang Xiaofei Guo Han dong School of Materials Science and Engineering Shanghai UniversityShanghai 200444China State Key Laboratory of Advanced Special Steel Shanghai UniversityShanghai 200444China

The microstructural evolution of a cold-rolled and intercritical annealed medium-Mn steel(Fe-0.10C-5Mn)was investigated during uniaxial tensile ***-situ observations under scanning electron microscopy,transmission electron microscopy,and X-ray diffraction analysis were conducted to characterize the progressive transformation-induced plasticity process and associated fracture initiation *** findings were discussed with the local strain measurements via digital image *** results indicated that Lüders band formation in the steel was limited to 1.5%strain,which was mainly due to the early-stage martensitic phase transformation of a very small amount of the less stable large-sized retained austenite(RA),which led to localized stress concentrations and strain hardening and further retardation of *** small-sized RA exhibited high stability and progressively transformed into martensite and contributed to a stably extended Portevin-Le Chatelier *** volume fraction of RA gradually decreased from 26.8%to 8.2%prior to *** the late deformation stage,fracture initiation primarily occurred at the austenite/martensite and ferrite/martensite interfaces and the ferrite phase.

关键词： medium-Mn steel retained austenite progressive transformation-induced plasticity effect local strain fracture initiation

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Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent LiCoO_(2)cathode material

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie Shi Changle Hou Jingjing dong dong chen Jianzhong Li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

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基于压缩感知的窄带高速自旋目标超分辨成像物理机理分析

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物理学报 2017年第3期66卷 337-346页

作者：李少东陈永彬刘润华马晓岩空军预警学院三系武汉430019

常规窄带雷达系统对高速自旋的空天目标成像时,方位脉冲重复频率通常难以满足采样率要求.而基于压缩感知(compressive sensing,CS)理论则可实现欠采样条件下窄带高速自旋目标的成像.本文对这一成像的物理机理进行分析和讨论.首先,构建... 详细信息

常规窄带雷达系统对高速自旋的空天目标成像时,方位脉冲重复频率通常难以满足采样率要求.而基于压缩感知(compressive sensing,CS)理论则可实现欠采样条件下窄带高速自旋目标的成像.本文对这一成像的物理机理进行分析和讨论.首先,构建方位欠采样回波模型,分析了该模型与CS理论的关系;其次,从物理角度分析基于CS理论可以保证欠采样条件下散射点准确重构的机理,给出欠采样倍数的理论下限值.仿真结果表明,欠采样条件下窄带雷达系统可实现对高速自旋目标二维成像,同时验证了基于CS的欠采样成像性能与欠采样倍数、等效强散射点个数以及波长等有关,与信号带宽无关等结论.

关键词：欠采样压缩感知高速自旋目标超分辨

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一个基于细观结构的粗粒料弹塑性本构模型

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岩土工程学报 2017年第5期39卷 777-783页

作者：刘斯宏邵东琛沈超敏王子健河海大学水利水电学院江苏南京210098 浙江省水利水电勘测设计院浙江杭州310002

针对粗粒料的剪胀特性和强度非线性,在经典弹塑性理论框架内,建立了一个基于细观结构的粗粒料弹塑性本构模型。模型采用基于颗粒材料细观结构变化推导得到的屈服函数,在此基础上由非相关联流动法则得到的剪胀方程,结合粗粒料典型的三轴... 详细信息

针对粗粒料的剪胀特性和强度非线性,在经典弹塑性理论框架内,建立了一个基于细观结构的粗粒料弹塑性本构模型。模型采用基于颗粒材料细观结构变化推导得到的屈服函数,在此基础上由非相关联流动法则得到的剪胀方程,结合粗粒料典型的三轴压缩试验结果,引入一种无黏性土的压缩模式,构造了能够统一描述粗粒料剪胀、剪缩特性的硬化参数。阐述了由常规三轴试验和等向压缩试验确定模型全部7个参数的方法。对3种粗粒料三轴压缩试验结果进行了预测,预测结果与试验结果吻合良好,说明模型能够合理地反映粗粒料的应力变形特性。

关键词：粗粒料细观结构屈服函数剪胀性弹塑性本构模型

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