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Development of an Indirect ELISA Using Recombinant Truncated Envelope Glycoprotein for Detection of Antibodies against Japanese Encephalitis Virus

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Animal Husbandry and Feed Science 2010年第1期2卷 38-42页

作者： ZU Li-chuang WANG jin-liang GUAN Yu SHEN Zhi-qiang dong Lin LI Jiao Shandong Ludu Bio-Technology Co. Ltd. Binzhou 256600 China Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600 China

[ Objective] To develop an indirect ELISA assay for detecting antibodies against envelope glycoprotein （ E protein） of Japanese encephalitis virus （JEV）. [ Method] Specific primers were designed according to JEV sequences published in the GenBank. The cDNA of JEV E gene （about 1 000 10p） was amplified by the RT-PCR with the specific primers. After sequencing analysis, the E gene was cloned into pET30a expression vector and expressed in E. coli BL21 （DE3） with the induction of IPTG. After denaturation, purification and renaturation, the recombinant protein was analyzed by the SDS-PAGE and the westem blotting. An indirect ELISA was developed to detect antibodies against JEV. [ Result] The E protein was mainly expressed in inclusion body. With the purified E protein, the indirect ELISA was developed and displayed good specificity, sensitivity and repeatability, [ Conclusion]The developed ELISA using the truncated E protein as antigen is a simple, convenient and rapid serological method for diagnosis, monitoring antibody level and epidemiological investigation of JEV.

关键词： Japanese encephalitis virus E protein Prokaryotic expression Enzyme linked immunosorbent assay Antibodies

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导管架海洋平台结构冰致强迫振动分析

哈尔滨工业大学学报 2009年第10期41卷 1-6页

作者：段忠东欧进萍周道成王刚哈尔滨工业大学土木工程学院哈尔滨150090 大连理工大学土木水利工程学院辽宁大连116024 深圳大学建筑与土木工程学院广东深圳518060

为了检验冰荷载振动理论的合理性并推动其在实际工程中的应用.首先介绍了动冰力的统一模型.然后利用动冰力函数的傅立叶级数展开,推导了在弯曲和屈曲冰力作用下,多自由度海洋平台结构反应的计算解析表达式;采用随机振动分析方法,对挤压... 详细信息

为了检验冰荷载振动理论的合理性并推动其在实际工程中的应用.首先介绍了动冰力的统一模型.然后利用动冰力函数的傅立叶级数展开,推导了在弯曲和屈曲冰力作用下,多自由度海洋平台结构反应的计算解析表达式;采用随机振动分析方法,对挤压随机动冰力作用的海洋平台结构进行了随机振动分析.最后,将方法应用于渤海某导管架结构简化模型冰振反应的分析,结果表明计算结果与实测结果具有很好的相一致性,验证了分析方法和统一动冰力模型可以较好地模拟实际的动冰力特性.

关键词：动冰力模型结构振动分析随机振动海洋平台

Generation of Ultrahigh-Velocity Collisionless Electrostatic Shocks Using an Ultra-Intense Laser Pulse Interacting with Foil-Gas Target

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Chinese Physics Letters 2019年第10期36卷 45-48页

作者： Shu-Kai He jin-Long Jiao Zhi-Gang Deng Feng Lu Lei Yang Fa-Qiang Zhang Ke-Gong dong Wei Hong Zhi-Meng Zhang Bo Zhang Jian Teng Wei-Min Zhou Yu-Qiu Gu Science and Technology on Plasma Physics Laboratory Research Center of Laser FusionChina Academy of Engineering PhysicsMianyang 621900 School of Physics Peking UniversityBeijing 100871

Ultra high-velocity collisionless shocks are generated using an ultra-intense laser interacting with foil-gas target,which consists of copper foil and helium *** energy of helium ions accelerated by shock and the proton probing image of the shock electrostatic field show that the shock velocity is 0.02c,where c is the light *** numerical and theory studies indicate that the collisionless shock velocity exceeding 0.1c can be generated by a laser pulse with picosecond duration and an intensity of 1020 W/*** system may be relevant to the study of mildly relativistic velocity collisionless shocks in astrophysics.

关键词： collision shock exceeding

A Retrospective Study of Directed Blood Donations in the Kasungu District of Malawi

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Open Journal of Blood Diseases 2018年第4期8卷 74-82页

作者： David S. Chung Fenjun Shen Sumin lee Taekwon Kong Jonathan Ko jin Young Choe Sang Min lee George Talama School of Medicine University of Virginia Charlottesville VA USA Department of Chemistry and Biochemistry UC Santa Barbara Santa Barbara CA USA Department of Human Biology Michigan State University East Lansing MI USA Department of Chemistry Washington University St. Louis MO USA School of Arts and Sciences NYU Yew York NY USA Seoul International School Seongnam South Korea La Canada High School La Canada CA USA Kasungu District Hospital Kasungu Malawi

Since the discovery of blood circulation and transfusion, there has been an insatiable demand for voluntary blood donations throughout the world. However, gathering blood donors has never been easy because eligible donors constitute only a fraction of the general population and are often reluctant to donate. This is especially challenging in underprivileged countries of sub-Saharan Africa such as Malawi whose nationally run blood transfusion service struggles to maintain hospital blood banks. As a result, hospitals turn to their local communities for directed donations. A retrospective analysis from January 2014 to June 2016 of directed blood donor data from two hospitals in the Kasungu District of Malawi was conducted. The analysis of 2134 donations was carried out with respect to sex, age, hemoglobin concentration, blood group, and presence of transfusion-transmissible infections. On average, donors were 30 years of age and predominately male. Blood group O+ constituted more than half of all directed blood donations. Ultimately, about one third of donations were unable to be utilized for transfusion.

关键词： Blood Type Blood Donor Data Malawi

Preparation and Releasing Characteristics of Controlled Release Potassium Permanganate

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Preparation and Releasing Characteristics of Controlled Rele...

人工岛填筑对紧邻高架桥桩基受力影响与填筑方案研究

中国材料大会2012

作者： Shuangshi dong jin Wang Yanlong Wang Jianbin Zhang Dandan Zhou Chunyan Shao Key laboratory of Groundwater and Environment Ministry of Education Jilin University

ISBN: (纸本)9783037856062;3037856068

To extend the duration of KMnO4 in the groundwater in situ remediation, controlled release oxidant was prepared by dispersing the KMnO4 particles in the molten paraffin wax. Preparation conditions, including KMnO4 particles size, ultrasonic treatment time and the weight ratio of KMnO4 to wax （P/W） were optimized. The results showed that the average volume particle size of 13.20 μm and ultrasonic treatment of 10 min contribute to the most uniform dispersion of the KMnO4 particles in the molten paraffin wax. The scanning electron microscope （SEM） images showed that the increased amount of KMnO4 caused the crushed structure of paraffin wax, which resulted in the higher porosity of wax to contain more KMnO4 . The KMnO4 loading amount increased significantly along with the increase of the P/W. However, the loading amount almost reached saturation by 609.63 mg/g when the P/W increased to 2/1. The releasing characteristics of the samples in the distilled water and the KMnO4 residual amount at different radial distance in the sample were examined by the bench scale tests. The releasing of KMnO4 could be divided into four periods and stable release took place after 40 d. Controlled release KMnO4 oxidant with P/W at 1/35 was used to remove COD in the leachate and 82.9% removal efficiency was obtained after 120 d treatment.

关键词： Potassium permanganate Controlled release Load amount Leachate.

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桥梁建设 2023年第3期53卷 135-142页

作者：董子龙傅鹤林侯禹辰卢金栋刘洪震中南大学土木工程学院湖南长沙410075 中铁隧道局集团有限公司广东广州511400 中铁隧道集团三处有限公司广东深圳518000 中铁隧道勘察设计研究院有限公司广东广州511400

深中通道工程东人工岛在广深沿江高速高架桥下方填筑施工,为了解其施工对紧邻高架桥的影响并选取合理的填筑方案,对采用不同分层填筑厚度、顺序、方向的填筑方案进行有限元分析比选;对最终采用的填筑方案中桩基水平位移、固结沉降进行监... 详细信息

深中通道工程东人工岛在广深沿江高速高架桥下方填筑施工,为了解其施工对紧邻高架桥的影响并选取合理的填筑方案,对采用不同分层填筑厚度、顺序、方向的填筑方案进行有限元分析比选;对最终采用的填筑方案中桩基水平位移、固结沉降进行监测,并对固结沉降简化计算方法进行研究。结果表明:填筑方案的选取应以桩基水平位移为主控项目;对于锁口钢管桩+内支撑保护形式的桩基,采用先围护内填筑较先围护外填筑的桩基水平位移小,横桥向交替填土较顺桥向交替填土的桩基水平位移小,分层填筑厚度0.5 m较1.0 m的桩基水平位移小,钢板桩+内支撑保护形式桩基也有类似规律;先内后外、横桥向分层交替填土、控制填筑厚度的填筑方案为最优方案。监测结果显示:该填筑方案可有效控制桩基水平位移,实现了既定控制目标;提出的固结沉降简化计算方法可用于可行性研究阶段中类似土层固结沉降的初步估算。

关键词：高架桥桩基桩基保护形式人工岛填筑方案水平位移固结沉降数值模拟监测

Robust Control for Super-Maneuver Flight Based on Adaptive Observer

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Robust Control for Super-Maneuver Flight Based on Adaptive O...

第30届中国控制与决策会议

作者： LI Bing-qian dong Wen-han XIE Wu-jie CHANG jin-yong Aeronautics and Astronautics Engineering College Air Force Engineering University

A robust control method for super-maneuver flight based on the adaptive observer has been designed in this paper to solve the uncertainty of aerodynamic parameter,external disturbance and model *** discontinuous projection adaptive law is adopted in the fast and slow loops to online estimate the uncertainty of aerodynamic *** external disturbance and model error are observed and compensated by the adaptive observer which designed by applying the adaptive law in the general observer,and then Lyapunov function is selected to analyze the stability of whole system to obtain final control *** digital simulation of super-maneuver flight proves that the controller can effectively resist interference caused by the uncertainty of aerodynamic parameter,external disturbance and model error,and enhance the robustness of the system.

关键词： super-maneuver flight adaptive observer discontinuous projection robustness digital simulation

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机构投资者、高管变更与股价波动

管理科学学报 2018年第7期21卷 113-126页

作者：王谨乐史永东东北财经大学应用金融研究中心大连116025 东北财经大学金融学院大连116025

基于2004年—2012年的中国上市公司数据,从高管变更的视角研究了机构投资者的公司治理效应,并尝试从投资者行为的角度解析高管变更事件对公司股价波动的影响机理.实证结果表明:第一,对于那些业绩亏损的公司,机构投资者的撤离能够形成较... 详细信息

基于2004年—2012年的中国上市公司数据,从高管变更的视角研究了机构投资者的公司治理效应,并尝试从投资者行为的角度解析高管变更事件对公司股价波动的影响机理.实证结果表明:第一,对于那些业绩亏损的公司,机构投资者的撤离能够形成较强的外部压力,迫使当任高管离职.而对于那些业绩仍然维持盈利的公司,机构投资者的施压作用则不明显.总体上,业绩好坏始终是公司高管被迫离任的主要原因,而机构投资者则是通过"用脚投票"的方式起到了推波助澜的作用.第二,高管强制变更会被机构投资者解读为负面信号,新高管的上任并不会挽回机构投资者的持股信心.第三,噪音交易者(中小个体投资者)倾向于将亏损公司的高管变更事件解读为利好消息,其踊跃买入行为则构成了公司股价在高管强制变更后发生波动加剧现象的直接原因.

关键词：机构投资者公司治理高管变更股价波动

Genome size evolution of the extant lycophytes and ferns

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Plant Diversity 2022年第2期44卷 141-152页

作者： Fa-Guo Wang Ai-Hua Wang Cheng-Ke Bai dong-Mei jin Li-Yun Nie AJ Harris Le Che Juan-JuanWang Shi-Yu Li Lei Xu Hui Shen Yu-Feng Gu Hui Shang Lei Duan Xian-Chun Zhang Hong-Feng Chen Yue-Hong Yan Key Laboratory of Plant Resources Conservation and Sustainable Utilization Guangdong Provincial Key Laboratory of Applied BotanySouth China Botanical GardenChinese Academy of SciencesGuangzhou510650China College of Life Sciences Shaanxi Normal UniversityXi'an710062China Eastern China Conservation Centre for Wild Endangered Plant Resources Shanghai Chenshan Botanical GardenShanghai201602China Key Laboratory of Environment Change and Resources Use in Beibu Gulf Ministry of EducationNanning Normal UniversityNanning530001China Department of Biology Oberlin CollegeOberlinOH44074USA State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing100093China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization the National Orchid Conservation Center of China and the Orchid Conservation&Research Center of Shenzhen518114ShenzhenChina Life Science and Technology College Harbin Normal UniversityHarbin150025China

Ferns and lycophytes have remarkably large ***,little is known about how their genome size evolved in fern *** explore the origins and evolution of chromosome numbers and genome size in ferns,we used flow cytometry to measure the genomes of 240 species(255 samples)of extant ferns and lycophytes comprising 27 families and 72 genera,of which 228 species(242 samples)represent new *** analyzed correlations among genome size,spore size,chromosomal features,phylogeny,and habitat type preference within a phylogenetic *** also applied ANOVA and multinomial logistic regression analysis to preference of habitat type and genome *** the phylogeny,we conducted ancestral character reconstruction for habitat types and tested whether genome size changes simultaneously with shifts in habitat *** found that 2 C values had weak phylogenetic signal,whereas the base number of chromosomes(x)had a strong phylogenetic ***,our analyses revealed a positive correlation between genome size and chromosome traits,indicating that the base number of chromosomes(x),chromosome size,and polyploidization may be primary contributors to genome expansion in ferns and *** sizes in different habitat types varied significantly and were significantly correlated with habitat types;specifically,multinomial logistic regression indicated that species with larger 2 C values were more likely to be *** habitat is inferred to be ancestral for both extant ferns and lycophytes,whereas transitions to other habitat types occurred as the major clades *** in habitat types appear be followed by periods of genomic *** on these results,we inferred that habitat type changes and multiple whole-genome duplications have contributed to the formation of large genomes of ferns and their allies during their evolutionary history.

关键词： Genome size Ferns Chromosome numbers Habitat type Whole-genome duplications Evolution