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Specific COX-2 inhibitor NS398 induces apoptosis in human liver cancer cell line HepG2 through BCL-2

World Journal of Gastroenterology 2005年第2期11卷 204-207页

作者： dong-shenghuang Ke-ZhenShen Jian-FengWei Thng-BoLiang Shu-SenZheng Hai-YangXie DepartmentofHepatobiliaryPancreaticSurgery TheFirstAffiliatedHospitalZhejiangUniversityCollegeofMedicineHangzhou310003ZhejiangProvinceChina

AIM: To evaluate the effects of NS-398, a cyclooxygenase-2 (COX-2) inhibitor, on the proliferation and apoptosis of HepG2 cells. METHODS: The effects of NS-398 on the proliferation of HepG2 cells were evaluated by MTT. DNA fragmentation gel analysis was used to analyze the apoptotic cells. DNA ploidy and apoptotic cell percentage were calculated by flow cytornetry. The expression of COX-2 and Bcl-2 mRNA was identified by competitive RT-PCR. Furthermore, expression level of Bcl-2 was detected using Western blot in HepG2 after treated with NS-398. RESULTS: NS-398 inhibited cell proliferation and induced apoptosis of HepG2 cells in a concentration-dependent manner. DNA ploidy analysis showed that S phase cells were significantly decreased with increase of NS-398 concentration. The quiescent GO/G1 phase was accumulated with decrease of Bcl-2 mRNA. Whereas NS-398 had no effect on the expression of COX-2 mRNA, and no correlations were found between COX-2 mRNA and HepG2 cell proliferation and apoptosis induced by NS-398 (r=0.056 and r=0.119, respectively). Bcl-2 protein level was inhibited after treated with NS-398. CONCLUSION: NS-398 significantly inhibits the proliferation and induces apoptosis of HepG2 cells. Mechanisms involved may be accumulation of quiescent GO/G1 phase and decrease of Bcl-2 expression.

关键词： Liver cancer NS-398 Bcl-2 protein COX-2

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Moment transfer factors for column-supported cast-in-situ hollow core slabs

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2012年第3期13卷 165-173页

作者： Hai-tao LI Andrew John DEEKS Li-xin LIU dong—shenghuang Xiao-zu SU Department of Building Engineering Nanj ing Forestry University Nanj ing 210037 China Faculty of Science Durham University Durham DH1 3LE England Department of Civil Engineering Zhengzhou University Zhengzhou 450002 China Department of Building Engineering Tongji University Shanghai 200092 China

Hollow core slabs are becoming of increasing interest as the construction industry attempts to minimise the impact of its activities on the environment. By forming voids in the interior of a concrete slab, the amount of concrete used can be reduced without significantly altering the capacity of the structure. In this study, we examined the inner force transfer mechanism of a column-supported cast-in-situ hollow core slab using finite element analysis. Both a hollow core slab and the corresponding solid slab were analysed using ANSYS and the results were compared. The orientation of the tube fillers causes the stiffness of the hollow slab to be orthotropic, potentially changing the distribution of load carried in the two orthogonal directions. Both the cross-section’s moments in the column strip and near the columns in the hollow core slab become larger than that in the solid floor. As well, the cross-section’s stiffness along the tube arrangement direction is larger than that of the radial cross-section, which causes the direction along the hole of the hollow core slab to carry more moment than the radial direction. The conversion factors of the two directions are proposed from the comparison for four typical areas of the hollow core slab, as are the moment distribution coefficients.

关键词： Reinforced concrete Cast-in-situ Hollow core slab Tube filler Finite element analysis

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三叶委陵菜的化学成分和药理作用研究进展

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湖南中医药大学学报 2020年第8期40卷 1039-1044页

作者：黄费炳吴嘉盛文兵彭彩云王炜张在其李斌陈胜璜湖南中医药大学药学院中药民族药物创新发展国际实验室中巴医药民族医药研究国际合作基地湖南长沙410208 湖南医药学院侗医药研究湖南省重点实验室湖南怀化418000

三叶委陵菜作为一种重要民间药物,在我国广泛分布于湖北、湖南、河北等地区。三叶委陵菜的主要成分为黄酮、鞣质、三萜、甾体等,具有肝保护、抗炎镇痛、抑菌、抗病毒、抗氧化、调节免疫等药理作用。本篇综述了目前三叶委陵菜的化学成分... 详细信息

三叶委陵菜作为一种重要民间药物,在我国广泛分布于湖北、湖南、河北等地区。三叶委陵菜的主要成分为黄酮、鞣质、三萜、甾体等,具有肝保护、抗炎镇痛、抑菌、抗病毒、抗氧化、调节免疫等药理作用。本篇综述了目前三叶委陵菜的化学成分及药理作用研究进展,以期为三叶委陵菜的进一步药效药理研究与开发提供基础。

关键词：三叶委陵菜黄酮萜类抗炎镇痛抗病毒

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