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Application of 3D bioprinting in the prevention and the therapy for human diseases

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Signal Transduction and Targeted Therapy 2021年第6期6卷 1681-1697页

作者： Hee-Gyeong Yi Hyeonji Kim Junyoung kwon Yeong-Jin Choi Jinah Jang dong-Woo Cho Department of Rural and Biosystems Engineering College of Agriculture and Life SciencesChonnam National University77 Yongbong-RoGwangju 61186Korea Department of Mechanical Engineering Pohang University of Science and Technology(POSTECH)77 Cheongam-RoPohangKyungbuk 37673Korea Department of Advanced Biomaterials Research Korea Institute of Materials Science(KIMS)797 ChangwondaeroChangwonKyungnam 51508Korea Department of Convergence IT Engineering POSTECH77 Cheongam-RoPohangKyungbuk 37673Korea Institute of Convergence Science Yonsei University50 Yonsei-RoSeoul 03722Korea

Rapid development of vaccines and therapeutics is necessary to tackle the emergence of new pathogens and infectious *** speed up the drug discovery process,the conventional development pipeline can be retooled by introducing advanced in vitro models as alternatives to conventional infectious disease models and by employing advanced technology for the production of medicine and cell/drug delivery *** this regard,layer-by-layer construction with a 3D bioprinting system or other technologies provides a beneficial method for developing highly biomimetic and reliable in vitro models for infectious disease *** addition,the high flexibility and versatility of 3D bioprinting offer advantages in the effective production of vaccines,therapeutics,and relevant delivery ***,we discuss the potential of 3D bioprinting technologies for the control of infectious *** also suggest that 3D bioprinting in infectious disease research and drug development could be a significant platform technology for the rapid and automated production of tissue/organ models and medicines in the near future.

关键词： printing prevention introducing

Neuroprotective effect of the ethanol extract of Artemisia capillaris on transient forebrain ischemia in mice via nicotinic cholinergic receptor

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Chinese Journal of Natural Medicines 2018年第6期16卷 428-435页

作者： Huiyoung kwon Ji Wook Jung Young Choon Lee Jong Hoon Ryu dong Hyun Kim Department of Medicinal Biotechnology College of Health SciencesDong-A UniversityBusan 49315Republic of Korea Department of Herbal Medicinal Pharmacology College of Herbal Bio-industryDaegu Haany UniversityKyungsan 38610Republic of Korea Institute of Convergence Bio-Health Dong-A UniversityBusan 49315Republic of Korea Department of Life and Nanopharmaeeutical Sciences Kyung Hee UniversityKyung Hee UniversitySeou102447Republic of Korea Department of Oriental Pharmaceutical Science College of PharmacyKyung Hee UniversityKyung Hee UniversitySeoul 02447Republic of Korea Kyung Hee East-West Pharmaceutical Research Institute Kyung Hee UniversityKyung Hee UniversitySeoul 02447Republic of Korea

Artemisia capillaris Thunberg is a medicinal plant used as a traditional medicine in many cultures. It is an effective remedy for liver problems including hepatitis. Recent pharmacological reports have indicated that Artemisia species can exert various neurological effects. Previously, we reported a memory-enhancing effect of Artemisia species. However, the mechanisms underlying the neuroprotective effect of A. capillaris(AC) are still unknown. In the present study, we investigated the effect of an ethanol extract of AC on ischemic brain injury in a mouse model of transient forebrain ischemia. The mice were treated with AC for seven days, beginning one day before induction of transient forebrain ischemia. Behavioral deficits were investigated using the Y-maze. Nissl and Fluoro-jade B staining were used to indicate the site of injury. To determine the underlying mechanisms for the drug, we measured acetylcholinesterase activity. AC(200 mg·kg-1) treatment reduced transient forebrain ischemia-induced neuronal cell death in the hippocampal CA1 region. The AC-treated group also showed significant amelioration in the spontaneous alternation of the Y-maze test performance, compared to that in the untreated transient forebrain ischemia group. Moreover, AC treatment showed a concentration-dependent inhibitory effect on acetylcholinesterase activity in vitro. Finally, the effect of AC on forebrain ischemia was blocked by mecamylamine, a nonselective nicotinic acetylcholine receptor antagonist. Our results suggested that in a model of forebrain ischemia, AC protected against neuronal death through the activation of nicotinic acetylcholine receptors.

关键词： Artemisia capillaris Transient forebrain ischemia Acetylcholinesterase Nicotinic acetylcholine receptor

Serum prostate-specific antigen as a predictor of prostate volume and lower urinary tract symptoms in acommunity-based cohort： a large-scale Korean screening study

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Asian Journal of Andrology 2013年第2期15卷 249-253,I0008页

作者： dong Soo Park Jong Jin Oh Jae Yup Hong Young kwon Hong Don Kyung Choi In Hyuck Gong Jin HoHwang Sung Won kwon Department of Urology CHA Bundang Medical Center CHA University Seongnam 463-712 Korea CHA Cancer Research Center Seou1135-081 Korea Department of Urology CHA Gangnam Medical Center CHA University Seou1135-081 Korea Korea Prostate Health Council Seou1137-070 Korea

The aim of this study is to assess the ability of serum prostate-specific antigen （PSA） to predict prostate volume （PV） and lower urinary tract symptoms （LUTS） represented by the international prostate symptom score （IPSS）. From January 2001 to December 2011, data were collected from men who first enrolled in the Korean Prostate Health Council Screening Program. Patients with a serum PSA level of 10 ng ml^-1 or age 〈40 years were excluded. Accordingly, a total of 34 857 men were included in our study, and serum PSA, PV and the IPSS were estimated in all patients. Linear and age-adjusted multivariate logistic analyses were used to assess the potential association between PSA and PV or IPSS. The predictive value of PSA for estimating PV and IPSS was assessed based on the receiver operating characteristics-derived area under the curve （AUC）. The mean PV was 29.9 ml, mean PSA level was 1.49 ng ml^-1 and mean IPSS was 15.4. A significant relationship was shown between PSA and PV, and the IPSS and PSA were also significantly correlated after adjusting by age. The AUCs of PSA for predicting PV ~20 ml, 〉25 ml and 〉35 ml were 0.722, 0.728 and 0.779, respectively. The AUCs of PSA for predicting IPSS 〉 7, 〉 13 and 〉 19 were 0. 548, 0.536 and 0. 537, respectively. Serum PSA was a strong predictor of PV in a community-based cohort in a large-scale screening study. Although PSA was also significantly correlated with IPSS, predictive values of PSA for IPSS above the cutoff levels were not excellent. Further investigations are required to elucidate the exact interactions between PSA and LUTS and between PSA and PV in prospective controlled studies. Such studies may suggest how PSA can be used to clinically predict PV and the IPSS.

关键词： lower urinary tract symptoms (LUTS) prostate prostate-specific antigen (PSA) prostate volume (PV)

Anti-cancer effect of ethylacetate fraction from Orostachys japonicus on HT-29 human colon cancer cells by induction of apoptosis through caspase-dependent signaling pathway

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Asian Pacific Journal of Tropical Medicine 2018年第5期11卷 330-335页

作者： Deok-Seon Ryu Hyun-Ji Lee Ji-Hye kwon dong-Seok Lee Department of Biomedical Laboratory Science Soonchunhyang University Department of Smart Foods and Drugs Graduate School of Inje University

Objective: To investigate the anti-colon cancer effects of ethylacetate fraction from Orostachys japonicus(0. japonicus) on HT-29 cancer cells. Methods: The viability of HT-29 cells was assayed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium(MTS) method. Apoptosis induction and cell cycle inhibition were confirmed by fluorescein isothiocyanate and propidium iodide staining using flow *** changes in the nucleus were observed, using a fluorescence microscope with4',6-diamidino-2-phenylindole(DAPI) nuclear staining. The expression levels of the upstream and downstream proteins involved in the anti-cancer mechanism were confirmed by Western blotting. Results: After treating HT-29 cells with different concentrations of ethylacetate fraction from O. japonicus, the viability of cells decreased in a concentration-dependent manner,while apoptosis induction and apoptotic body formation increased. Cell cycle analysis showed that the arrest occurred at the sub-G_1 and S phase. Among the upstream and downstream proteins involved in anti-cancer activity, the level of B cell lymphoma-2 decreased, and the bcl-2-associated x protein increased. The level of pro-caspase-3, pro-caspase-8, and pro-caspase-9 decreased, while the level of cleaved-caspase-3, cleaved-caspase-8, and cleaved-caspase-9 increased. Moreover, the phosphorylation, that is, activation of extracellular signal regulated kinase 1/2, Jun-N-terminal kinase, and p38 increased. Conclusions: Combining the above results, it is thought that the survival of HT-29 cells is suppressed by ethylacetate fraction from0. japonicus through mitochondrial regulation-induced caspase cascade activation, induction of apoptosis and cell cycle arrest.

关键词： Orostachys japonicus HT-29 human colon cancer cells Anti-cancer activity Apoptosis Caspase cascade

ybrid Management for Supraceliac Aortic Aneurysm in a High-Risk Patient

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Chinese Medical Journal 2018年第14期131卷 1757-1758页

作者： Jun Seong kwon Jeong Kye Hwang Sun Cheol Park Sang dong Kim Department of Surgery Division of Vascular and Transplant Surgery College of Medicine The Catholic University of Korea Seou1403-720 Korea

To the Editor： An 84-year-old male was referred from a local clinic for an incidental thoracoabdominal aortic aneurysm （TAAA） presenting in a supraceliac aortic aneurysm （SCAA） with maximal diameter of 6.0 cm in spine magnetic resonance imaging. He had a medical history of hypertension and chronic obstructive pulmonary disease, and was a 30 pack-years＇ smoker. On physical exalnination, his vital signs were stable and abdomen was soft without pain and tenderness. Laboratory findings showed white blood cell count of 4840/μl, serum creatinine of 9 mg/L, erythrocyte sedimentation rate of 16 mm/h, and c-reactive protein of 13.24 mg/L.

关键词： REPAIR

Simple keratectomy and corneal tattooing for limbal dermoids:results of a 3-year study

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International Journal of Ophthalmology(English edition) 2013年第4期6卷 463-466页

作者： dong Min Cha Kyung-Hoon Shin Keun Ho Kim Ji-Won kwon Department of Ophthalmology Jeju National University College of Medicine Jeju National University Hospital Department of Ophthalmology Myongji HospitalKwandong University College of Medicine

AIM:To evaluate and report the efficacy of combined surgical excision and corneal tattooing in patients with limbal dermoids.·METHODS:In a retrospective interventional case series,9 eyes of 8 patients were treated with combined surgery of simple keratectomy and corneal tattooing for limbal *** records,including best-corrected visual acuity,anterior segment photography,demographic,clinical data,and follow-up information were reviewed.·RESULTS:The mean follow up period in this study was 50±15(range 36-77) *** was no evidence of infection or recurrent limbal dermoids in any of the eyes during the follow-up *** patients achieved good cosmetic outcomes with no complications.·CONCLUSION:Simple keratectomy and corneal tattooing of limbal dermoids could be an alternative option for surgery,especially when a donor cornea is not available.

关键词： corneal tattooing limbal dermoid simple keratectomy

Silver-palladium alloy deposited by DC magnetron sputtering method as lubricant for high temperature application

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中国有色金属学会会刊：英文版 2009年第4期19卷 1001-1004页

作者： Jung-Dae kwon Sung-Hun LEE Koo-Hyun LEE Jong-Joo RHA Kee-Seok NAM Sang-Hoon CHOI dong-Min LEE dong-Il KIM Materials Processing Division Korea Institute of Materials Science Technology Research & Development Institute Korea Plant Service and Engineering

The silver-palladium(Ag-Pd) alloy coating as a solid lubricant was investigated for its application to the high temperature stud bolts used in nuclear power plants.A hex bolt sample was prepared in the following steps:1) bolt surface treatment using alumina grit blasting for cleaning and increasing the surface area;2) nickel(Ni) film coating as a glue layer on the surface of the bolt;and 3) Ag-Pd alloy coating on the Ni *** films were deposited by using a direct current(DC) magnetron sputtering *** thickness and composition of the Ag-Pd alloy film have effect on the friction coefficient,which was determined using axial force measurement.A 500 nm-thick Ag-Pd(80-20,molar ratio) alloy film has the lowest friction coefficient of 0.109.A cyclic test was conducted to evaluate the durability of bolts coated with either the Ag-Pd(80-20) alloy film or N-5000 *** a cycle,the bolts were inserted into a block using a torque wrench,which was followed by heating and *** only one cycle,it was not possible to remove the bolts coated with the N-5000 oil from the ***,the bolts coated with the Ag-Pd(80-20) alloy could be easily removed up until 15 cycles.

关键词：银钯合金直流电高温润滑剂沉积磁控溅射法固体润滑涂层高温螺栓

Effects of core and non-dominant arm strength training on drive distance in elite golfers

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Journal of Sport and Health Science 2016年第2期5卷 219-225页

作者： dong Jun Sung Seung Jun Park Sojung Kim Moon Seok kwon Young-Tae Lim Division of Sport Science Konkuk University College of Physical Education Kyung Hee University Suwon

Background： Various training schemes have sought to improve golf-related athletic ability. In the golf swing motion, the muscle strengths of the core and arms play important roles, where a difference typically exists in the power of arm muscles between the dominant and non- dominant sides. The purposes of this study were to determine the effects of exercises strengthening the core and non-dominant arm muscles of elite golf players （handicap 〈 3） on the increase in drive distance, and to present a corresponding training scheme aimed at improving golf performance ability. Methods： Sixty elite golfers were randomized into the control group （CG, n = 20）, core exercise group （CEG, n = 20）, and group receiving a combination of muscle strengthening exercises of the non-dominant arm and the core （NCEG, n = 20）. The 3 groups conducted the corresponding exercises for 8 weeks, after which the changes in drive distances and isokinetic strength were measured. Results： Significant differences in the overall improvement of drive distance were observed among the groups （p 〈 0.001）. Enhancement of the drive distance of NCEG was greater than both CG （p 〈 0.001） and CEG （p = 0.001）. Except for trunk flexion, all variables of the measurements of isokinetic strength for NCEG also showed the highest values compared to the other groups. Examination of the correlation between drive distance and isokinetic strength revealed significant correlations of all variables except trunk flexion, wrist extension, and elbow extension. Conclusion： The combination of core and non-dominant arm strength exercises can provide a more effective specialized training program than core alone training for golfers to increase their drive distances.

关键词： Core exercise Drive distance Elite golfer Isokinetic strength Non-dominant arm strength exercise

The inhibition effect of Chlorpromazine against the β-lactam resistance of MRSA

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Asian Pacific Journal of Tropical Medicine 2016年第6期9卷 526-530页

作者： Ryong Kong Ok-Hwa Kang Yun-Soo Seo Su-Hyun Mun Tian Zhou dong-Won Shin dong-Yeul kwon BK21 Plus Team Professional Graduate School of Oriental Medicine Wonkwang University Department of Oriental Pharmacy College of Pharmacy Wonkwang Oriental Medicines Research Institute Institute of Biotechnology Wonkwang University Department of Oriental Medicine Resources College of Bio Industry Science Sunchon National University

Objective:To investigate the gene related to β-lactam resistance and to confirm the mechanism about a synergy effect between CPZ and β-lactam ***:To measure antibacterial activity,we performed a minimum inhibitory concentration(MIC) and synergy *** electron microscopy(TEM) was used in morphological *** analyze gene expression,we conducted reverse transcriptase polymerase chain reaction(PCR).Results:We confirmed a synergy effect between CPZ and β-lactam ***,we observed that CPZ affect the cell envelope of MRS A by using *** the gene level,CPZ reduced the expression of resistance ***:Through this result,we hypothesize that a decrease of resistance factor expressions was caused by CPZ because it disrupts the activity of a sensor protein located in the cell membrane.

关键词： Chlorpromazine PBP2a β-lactamase mecA blaZ MRSA