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Studies of polyol production by the yeast Yarrowia lipolytica growing on crude glycerol under stressful conditions

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Carbon Resources Conversion 2024年第3期7卷 17-31,F0004页

作者： Eleni-Stavroula Vastaroucha Nikolaos G.Stoforos George Aggelis seraphim Papanikolaou Department of Food Science and Human Nutrition Agricultural University of Athens75 Iera Odos11855AthensGreece Unit of Microbiology Department of BiologyUniversity of Patras26504 Rio PatrasGreece

Crude glycerol,the principal by-product of biodiesel production process,was employed as substrate by three wild-type Yarrowia lipolytica strains(ACA-YC 5030,LMBF 20 and NRRL Y-323).Stressful conditions(low pH value=2.0±0.3,low incubation temperature T=20±1℃,non-aseptic conditions)were ***-esting production of yeast biomass and polyols(***,mannitol and arabitol)was noted at pH=2.0±0.3 and T=20±1℃.Strains failed to produce significant quantities of cellular lipid,while variable quantities of intra-cellular polysaccharides were *** under previously pasteurized media supported significant biomass and polyols production for most of the tested strains,while only one strain(NRRL Y-323),managed to produce polyols at media that were not previously thermally treated at *** production of mannitol was favored at low initial glycerol(Glol0)concentrations,whereas higher Glol0 quantities favored the biosynthesis of *** the strain NRRL Y-323,highly aerated/agitated bioreactor trials showed different physiological profiles as compared to the respective flask ***,in flask experiments with the strain NRRL Y-323 at high Glol0 amounts(≈140 g/L)at low medium pH(=2.0±0.3),a significant production of polyols(=84.2 g/L)with the corresponding remarkable conversion yield on glycerol consumed=62%w/w was *** application:Renewable and biodegradable fuels,such as biodiesel,are safer and environmentally friendlier than the conventional petroleum *** is a cost-effective substrate obtained as the main side-product from biodiesel production process and is currently being employed in the realm of Industrial Microbi-ology and Biotechnology to produce metabolic products with added *** research focuses on using glycerol as a starting substrate for biotechnological conversions aiming at producing,amongst other compounds,polyols,microbial biomass,citric acid,*** selected strains of the Generall

关键词： Biomass Glycerol Polyols Yarrowia lipolytica

Recent advances in the production of single cell protein from renewable resources and applications

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Carbon Resources Conversion 2024年第2期7卷 38-49页

作者： Danai Ioanna Koukoumaki Erminta Tsouko seraphim Papanikolaou Zacharias Ioannou Panagiota Diamantopoulou Dimitris Sarris Laboratory of Physico-Chemical and Biotechnological Valorization of Food By-Products Department of Food Science&NutritionSchool of EnvironmentUniversity of the AegeanLeoforos Dimokratias 66Myrina 81400LemnosGreece Department of Food Science and Human Nutrition Agricultural University of Athens75Iera Odos11855 AthensGreece Laboratory of Edible Fungi Institute of Technology of Agricultural ProductsHellenic Agricultural Organization-Dimitra1Sof.Venizelou14123 LykovrysiGreece

Single Cell Protein(SCP)refers to dry cells of microorganisms,and it constitutes a highly promising and alternative protein source for multiple *** presents a rich nutritional profile containing valuable amino acids and fatty acids,nucleic acids,minerals,and several *** businesses worldwide have introduced SCP into their production cycles,hence expanding the scope of its application in value added market chains such as the edible food *** is produced by a plethora of microorganisms,including fungi,yeasts,bacteria,and algae while many of them are Generally Recognized as Safe(GRAS).Selected microbial strains present satisfying growth capability with high yields when cultivated in renewable ***,production rates and process sustainability could be enhanced via the valorization of industrial and agricultural wastes as the nutrient sources,combined with optimization of process parameters,i.e fermentation mode and feeding strategy,pH,temperature,C/N ratio,agitation rate and oxygen *** review addresses the latest developments made towards the SCP production,highlighting efficient microbial SCP producers,and production systems that valorize solid and liquid streams from several agricultural *** applications,challenges in sensorial-,and safety-aspects as well as consumers perception issues of SCP incorporation into food-related matrices are also discussed.

关键词： Alternative protein Sustainability Sensorial aspects Safety Food and feed Packaging applications

Enhanced fat degradation following the addition of a Pseudomonas species to a bioaugmentation product used in grease traps

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Journal of Environmental Sciences 2019年第3期31卷 174-188页

作者： Markella Tzirita seraphim Papanikolaou Bríd Quilty School of Biotechnology Dublin City University (DCU) Department of Food Science and Human Nutrition Agricultural University of Athens National Institute for Cellular Biotechnology (NICB) DCU

The presence of fats, oils and greases(FOGs) in wastewater can lead to many problems including blockages. Investigation of a bioaugmentation product, consisting of Bacillus spp., to degrade butter(1%, W/V) and olive oil(1%, V/V) was performed in aerobic batch cultures for 13-day incubation. Gravimetric analysis of the remaining substrates showed slowly degradation of the oil after a 2-day lag, but no degradation of the butter. Addition of a Pseudomonas putida strain CP1 to the Bacillus spp. population promoted rapidly degradation of both fats after7 days of incubation. High fat accumulation revealed the potential use of the new bacterial mixture for production of added-value compounds. Lipase production only by the Bacillus *** with the analysis of the remaining lipids with thin layer chromatography and gas chromatography, suggested that the Bacillus spp. mainly only hydrolyzed the fat. The breakdown products were metabolized by the Pseudomonas putida CP1 performing preferential utilization of unsaturated fatty acids. Investigation of population dynamics using selective plating and a labeled Pseudomonas putida CP1::Tn7-gfp showed domination of the latter. The new mixture performed a successful cooperation with good potential for FOG treatment and an aggregative response desirable to fat degradation in grease traps.

关键词： Bacillus Bioaugmentation Biodegradation FOG Lipase Pseudomonas

Biotechnological valorization of biodiesel-derived glycerol: Trials with the non-conventional yeasts Yarrowia lipolytica and Rhodosporidium sp

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Carbon Resources Conversion 2021年第1期4卷 61-75页

作者： Sofia Sarantou Nikolaos G.Stoforos Ourania Kalantzi seraphim Papanikolaou Department of Food Science&Human Nutrition Agricultural University of Athens75 Iera Odos11855 AthensGreece

Four non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium were cultivated under nitrogen-limited conditions in shake flasks with biodiesel-derived glycerol employed as sole *** strains *** DSM 4444 and *** ACA-DC 5033 displayed better performances and were further *** initial glycerol(Gly0)≈50 g/L,the strain ACA-DC 5033 produced dry cell weight(DCW)≈7.0 g/L,and also secondary extra-cellular metabolites as citric acid(CA)at≈16 g/L and polyols(Pol)(mannitol,erythritol and arabitol)at≈21 g/*** double-media(besides nitrogen also Mg was limiting)for Gly0≈50 g/L,a shift towards CA production occurred(CAmax≈33 g/L).The strain DSM 4444 at Gly0≈50 g/L produced DCW=18.1 g/L containing lipids=30.3%in *** single nitrogen-limited media with Gly0≈90 g/L,the strain ACA-DC 5033 produced Pol≈48 g/L and CA≈20 g/L,while the strain DSM 4444 produced DCW=27.3 g/L containing lipid=54.5%w/*** the late growth phases,metabolites were *** growth phase(trophophase)and phase of secondary metabolite synthesis(idiophase)were successfully simulated with the aid of a modified Velhlust-Aggelis *** extraction process was studied for these two strains by using two different extraction *** lipids contained mostly oleic acid,constituting suitable precursors for the synthesis of 2nd generation biodiesel.

关键词： Biodiesel-derived glycerol Microbial lipid Polyols Rhodosporidium toruloides Yarrowia lipolytica

Sustainable arabitol production by a newly isolated Debaryomyces prosopidis strain cultivated on biodiesel-derived glycerol

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Carbon Resources Conversion 2022年第1期5卷 92-99页

作者： Rosanina Filippousi Erminta Tsouko Kerryane Mordini Dimitrios Ladakis Apostolis AKoutinas George Aggelis seraphim Papanikolaou Department of Food Science and Human Nutrition Agricultural University of Athens75 Iera Odos11855 AthensGreece Unit of Microbiology Department of BiologyUniversity of Patras26504–Rio PatrasGreece

The purpose of this research was the bioconversion of biodiesel-derived glycerol to arabitol through the adoption of sustainable *** production by Debaryomyces prosopidis FMCC Y69 was significantly affected by initial glycerol *** highest arabitol concentration of 45.5 g/L was achieved in the case of 120 g/L initial glycerol combined with the highest yield(0.379 g arabitol/g initial glycerol).The yeast strain showed a non-typical oleaginous behavior with low accumulation of lipids(up to 20.9%w/w)despite nitrogen-limited condition that prevailed in the fermentation ***(IPs)were considerably high with a yield on the produced dry cell weight(YIPs/X)(=40.9%w/w)when 85 g/L of glycerol was utilized as the starting *** fermentation efficiency was further improved when the optimal carbon to nitrogen(C/N)ratio was *** specifically,arabitol production was maximized(reaching 56.5 g/L)at a C/N ratio of 158 moles/moles while yield and productivity were also enhanced with respective values of 0.48 g arabitol/g consumed glycerol and 0.112 g/*** increase in C/N ratios led to a decrease in cell dry weight(DCW)concentrations,which is rationally associated with decreased nitrogen availability in the growth ***/X values were remarkably high(23.1–26.1%w/w)at the end of each fermentation with the highest concentration in absolute values of 7.3 g/L achieved at the lowest C/N ratio employed(i.e.,79 moles/moles).This study demonstrated the efficient arabitol production along with other value-added metabolites,such as IPs and cellular lipids,implementing biotechnology and renewable resources and thus contributing to the development of circular economy technologies.

关键词： Bioprocessing Renewable resources Newly isolated yeast Arabitol production Value added metabolites