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A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in Er+breast cancer cells

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Microsystems & Nanoengineering 2024年第1期10卷 287-299页

作者： Braulio Andrés Ortega Quesada Jonathan Cuccia rachael Coates blake Nassar Ethan Littlefield Elizabeth C.Martin Adam T.Melvin Cain Department of Chemical Engineering Louisiana State UniversityBaton RougeLA 70803USA Department of Chemical and Biological Engineering Clemson UniversityClemsonSC 29634USA Biological and Agricultural Engineering Louisiana State UniversityBaton RougeLA 70803USA Department Medicine Section Hematology and Medical OncologyTulane UniversityNew OrleansLA 70118USA

Metastatic breast cancer leads to poor prognoses and worse outcomes in patients due to its invasive behavior and poor response to *** is still unclear what biophysical and biochemical factors drive this more aggressive phenotype in metastatic cancer;however recent studies have suggested that exposure to fluid shear stress in the vasculature could cause *** this study a modular microfluidic platform capable of mimicking the magnitude of fluid shear stress(FSS)found in human vasculature was designed and *** device provides a platform to evaluate the effects of FSS on MCF-7 cell line,an estrogen receptor positive(Er+)breast cancer cell line,during circulation in the *** of the effects of FSS on MCF-7 cells was carried out utilizing two approaches:single cell analysis and bulk *** single cell analysis,cells were trapped in a microarray after exiting the serpentine channel and followed by immunostaining on the device(on-chip).Bulk analysis was performed after cells were collected in a microtube at the outlet of the microfluidic serpentine channel for western blotting(off-chip).It was found that cells exposed to an FSS magnitude of 10 dyn/cm^(2) with a residence time of 60 s enhanced expression of the proliferation marker Ki67 in the MCF-7 cell line at a single cell *** understand possible mechanisms for enhanced Ki67 expression,on-chip and off-chip analyses were performed for pro-growth and survival pathways ErK,AKT,and JAK/*** demonstrated that after shearing the cells phosphorylation of p-AKT,p-mTOr,and p-STAT3 were ***,there was no change in p-ErK1/*** is a mediator of Er rapid signaling,analysis of phosphorylated Erαwas carried out and no significant differences between sheared and non-sheared populations were *** together these results demonstrate that FSS can increase phosphorylation of proteins associated with a more aggressive phenotype in circulating cancer *** findin

关键词： fluid breast estrogen

近年来珠三角地区大气中痕量氟氯烃(CFCs)的浓度水平与变化特征

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地球与环境 2006年第4期34卷 19-24页

作者：张芳王新明李龙凤易志刚周凌晞 donald r blake 盛国英傅家谟中国科学院广州地球化学研究所有机地球化学重点实验室广东广州510640 中国气象科学研究院中国气象局大气成分观测与服务中心中国气象局大气化学重点开放实验室北京100081 加州大学Irvine分校化学系

用预浓缩-GC/MS方法研究了珠江三角洲大气中的CCl3F、CFC-12、CFC-113和CFC-114等4种痕量氟氯烷烃气体。结果表明,2005年珠江三角洲背景点鼎湖山大气中CFC-12和CFC-11的年平均浓度高于全球本底站,说明珠三角地区还存在一定CFC-11和CFC-1... 详细信息

用预浓缩-GC/MS方法研究了珠江三角洲大气中的CCl3F、CFC-12、CFC-113和CFC-114等4种痕量氟氯烷烃气体。结果表明,2005年珠江三角洲背景点鼎湖山大气中CFC-12和CFC-11的年平均浓度高于全球本底站,说明珠三角地区还存在一定CFC-11和CFC-12的排放源;CFC-113浓度水平则与全球本底站点浓度接近,且CFC-113和CFC-114在广州城区与鼎湖山差别不显著,表明区内其排放源强度应很小。观测日内广州和鼎湖山大气中四种CFCs的日变化幅度均较小,无明显的昼夜变化规律。广州市CFCs总体呈夏秋高、冬春低的特征,与城区致冷设备高温季节使用频率较大有关;鼎湖山则呈冬春高、夏秋低的特征,主要受扩散作用和季风的影响。初步分析显示,从1997年到2005年,CFC-11、CFC-12和CFC-114浓度总体呈现先上升后缓慢降低的趋势,而CFC-113的浓度一直逐年下降。

关键词：氟氯烃卤代烃破坏臭氧层物质珠江三角洲

珠三角地区大气中HCFC-22的浓度观测和变化趋势初步研究

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环境科学学报 2006年第6期26卷 987-991页

作者：张芳王新明易志刚李龙凤陈尊裕陈鲁言 donald r.blake 中国科学院广州地球化学研究所有机地球化学国家重点实验室香港理工大学土木与结构工程学系加州大学Irvine分校化学系

通过罐采样和预浓缩色谱/质谱方法,对珠三角地区广州城区和鼎湖山大气中HCFC22进行了初步的观测和分析.结果表明,2005年广州城区HCFC22年平均体积分数达到(479.5±241.4)×10-12,鼎湖山仅为(244.4±51.3)×10-12.广州城区大气HCFC22日... 详细信息

通过罐采样和预浓缩色谱/质谱方法,对珠三角地区广州城区和鼎湖山大气中HCFC22进行了初步的观测和分析.结果表明,2005年广州城区HCFC22年平均体积分数达到(479.5±241.4)×10-12,鼎湖山仅为(244.4±51.3)×10-12.广州城区大气HCFC22日变化幅度夏秋季节相对较大,而鼎湖山日变化不明显;广州城区大气HCFC22的体积分数夏秋季节高于冬春季节,鼎湖山则相反.与城区HCFC22排放的季节性有关,夏秋季节广州城区与鼎湖山HCFC22体积分数差别很大,冬春季节则比较接近.初步研究结果表明,广州城区和鼎湖山大气HCFC22的体积分数呈现快速增长趋势,年增长率分别达到6.57%和7.28%,约为一些全球基准观测站(NOAA/CMDL)的1.9～2.8倍.

关键词： HCFC-22 珠江三角洲日变化季节变化增长率

岭南地区大气的CFC-11,12,113以及HCFC-22的浓度水平以及变化趋势

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岭南地区大气的CFC-11,12,113以及HCFC-22的浓度水平以及变化趋势

srNAminer:多功能的植物小rNA分析工具

中国气象学会2006年年会“大气成分与气候、环境变化”分会场

作者：张芳王新明李龙凤易志刚 donald r.blake 盛国英傅家谟中国科学院广州地球化学研究所有机地球化学重点实验室中国科学院广州地球化学研究所有机地球化学重点实验室中国科学院广州地球化学研究所有机地球化学重点实验室中国科学院广州地球化学研究所有机地球化学重点实验室加州大学Irvine分校化学系中国科学院广州地球化学研究所有机地球化学重点实验室中国科学院广州地球化学研究所有机地球化学重点实验室

珠三角地区CFC-11,12,113以及HCFC-22总体上在人口密集的中心城市(深圳,香港,澳门,广州)浓度较高。17个广州-海南沿途观测点4种气体(除HCFC-22外)的浓度分布整体较为集中,其中CFC-11和CFC-12在个别观测点(如新梅和关塘)出现异常极大值。... 详细信息

珠三角地区CFC-11,12,113以及HCFC-22总体上在人口密集的中心城市(深圳,香港,澳门,广州)浓度较高。17个广州-海南沿途观测点4种气体(除HCFC-22外)的浓度分布整体较为集中,其中CFC-11和CFC-12在个别观测点(如新梅和关塘)出现异常极大值。2001年3月7日至3月8日,广州3种CFCs(除CFC-113外)以及HCFC-22的日变化幅度较大,但未呈明显的昼夜变化规律;鼎湖山日变化幅度小,整体变化趋势平缓。广州CFCs和HCFC-22均表现出较明显的季节差异性,峰值出现在气温较高的夏秋季节,各卤代烃的月均值与气温呈正相关,主要是因为这类物质作为制冷剂、发泡剂,气雾剂等,其相关设备在气温高的夏秋季使用频率较高。初步估计表明,从1997～2005年,CFC-12和CFC-11呈先上升后缓慢下降的变化趋势,只有CFC-113浓度逐年下降;HCFC-22浓度则持续不断上升。

关键词：臭氧层损耗物质温室气体氟氯烃(CFCs) HCFC-22 浓度水平变化趋势

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Science Bulletin 2024年第6期69卷 784-791页

作者：李冠良陈程杰陈佩珂 blake C.Meyers 夏瑞 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of HorticultureSouth China Agricultural UniversityGuangzhou 510640China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510640China Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China Ministry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou 510640China Center Saint LouisMO 63132USA Division of Plant Science and Technology University of Missouri-ColumbiaColumbiaMO 65211USA

Small rNAs(srNAs),found extensively in plants,play an essential role in plant growth and *** various srNA analysis tools have been developed for plants,the use of most of them depends on programming and command-line environments,which is a challenge for many wet-lab ***,current srNA analysis tools mostly focus on the analysis of certain type of srNAs and are resource-intensive,normally demanding an immense amount of time and effort to learn the use of numerous tools or scripts and assemble them into a workable pipeline to get the final ***,we present srNAminer,a powerful stand-alone toolkit with a user-friendly interface that integrates all common functions for the analysis of three major types of plant srNAs:microrNAs(mirNAs),phased small interfering rNAs(phasirNAs),and heterochromatic sirNAs(hc-sirNAs).We constructed a curated or"golden"set of MIrNA and PHAS loci,which was used to assess the performance of srNAminer in comparison to other existing *** results showed that srNAminer outperformed these tools in multiple aspects,highlighting its *** addition,to enable an efficient evaluation of srNA annotation results,we developed Integrative Genomics Viewer(IGV)-srNA,a modified genome browser optimized from IGV and we incorporated it as a functional module in ***-srNA can display a wealth of srNA-specific features,enabling a more comprehensive understanding of srNA *** and IGV-srNA are both platform-independent software that can be run under all operating *** are now freely available at https://***/kli28/srNAminer and https://***/CJchen/IG V-srNA.

关键词： srNAminer srNA analysis mirNA PhasirNA hc-sirNA IGV-srNA

How University Athletics Can Impact Mental Health among Student Athletes at the University of Evansville

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Health 2024年第3期16卷 218-233页

作者： Claudia Hollis blake Johnson Salma Gonzalez Sarah Harness College of Education and Health Sciences University of Evansville Evansville USA

In recent years, the NCAA student athlete population in the United States has surpassed 500,000, and is continuing to rise each year [1]. These student athletes work their entire lives academically and athletically to reach the ultimate goal: competing in university athletics. However, when these athletes reach university, they are met with non-stop training, homework, exams, and evolving social lives. We have conducted a study at the University of Evansville evaluating how participation in university athletics may impact mental health status among these student athletes, as well as measuring players’ awareness of accessible mental health resources. Over fifty percent of participants reported experiencing at least one mental health condition while competing in their sport;eighty percent reported having knowledge of the mental health resources available to them on campus, however, nearly thirty percent of those knowledgeable reported not knowing how to access these resources. This has indicated a gap in awareness and utilization of mental health resources among student athletes at the University of Evansville.

关键词： Student Athlete Mental Health Mental Health resources

Asbestos Exposure during Gasket removal from Aircraft Engine

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Occupational Diseases and Environmental Medicine 2024年第2期12卷 59-67页

作者： Charles L. blake Kevin M. Guth raymond D. Harbison Apex Companies Marietta USA Center for Environmental and Occupational risk Analysis and Management College of Public Health University of South Florida Tampa USA

We have previously evaluated asbestos exposure associated with various maintenance procedures on light aircraft. The purpose of this study was to evaluate asbestos exposure during engine maintenance on light aircraft. This test was designed to evaluate the potential for asbestos exposure to mechanics and others who remove asbestos-containing engine gaskets from reciprocating style aircraft engines. Utilized in this test was an air cooled, horizontally opposed, aviation gasoline burning engine, assembled during 1986 and operated intermittently up into 2015, having accumulated 1680 hours run time. Nearly 75% of the asbestos-containing gaskets installed during 1986 were still in place at the time of testing. Chrysotile asbestos contents of such gaskets ranged from 55% to 60% by area, for those of sheet style and 5% by area, for the spiral wound metal/asbestos style. Despite the levels of effort required to effect gasket removals, the professional aircraft mechanic was not exposed to airborne asbestos fibers at the lower limits of sampling and analytical detection achieved;all of which were substantially less than the current Occupational Safety and Health Administration Permissible Exposure Limits for asbestos. The results of this testing indicate an absence of gasket related asbestos exposure risk to mechanics who work with light aircraft engines, including those having asbestos-containing gaskets. These results are consistent with the findings of Mlyarek and Van Orden who studied the asbestos exposure risk occasioned during overhaul of larger radial style reciprocating aircraft engines [1].

关键词： Asbestos Exposure Gaskets Aircraft Engine Friction Materials

Does the use of double hormone replacement therapy for trauma patient organ donors improve organ recovery for transplant

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World Journal of Transplantation 2024年第2期14卷 119-125页

作者： Eden M Gallegos Tanner reed Paige Deville blake Platt Claudia Leonardi Lillian Bellfi Jessica Dufrene Saad Chaudhary John Hunt Lance Stuke Patrick Greiffenstein Jonathan Schoen Alan Marr Anil Paramesh Alison A Smith Department of Physiology Louisiana State University Health Sciences Center New OrleansNew OrleansLA 70124United States Department of Medicine Louisiana State University Health Sciences Center New OrleansNew OrleansLA 70112United States Department of Surgery Louisiana State University Health Sciences Center New OrleansNew OrleansLA 70112United States Department of Trauma Surgery Tacoma General HospitalTacoma 98405United States Department of Public Health Louisiana State University Health Sciences Center New OrleansNew OrleansLA 70112United States Department of Donation Services Louisiana Organ Procurement AgencyCovingtonLA 70433United States Department of Surgery Tulane Transplant InstituteTulane University School of MedicineNew OrleansLA 70112United States

BACKGrOUND With an ongoing demand for transplantable organs,optimization of donor management protocols,specifically in trauma populations,is important for obta-ining a high yield of viable organs per *** management of brain-dead potential organ donors(BPODs)is controversial,leading to heterogeneous clinical management *** studies have shown that when levo-thyroxine was combined with other treatments,including steroids,vasopressin,and insulin,BPODs had better organ recovery and survival outcomes were increased for transplant *** To determine if levothyroxine use in combination with steroids in BPODs increased the number of organs donated in trauma *** A retrospective review of adult BPODs from a single level 1 trauma center over ten years was *** criteria included patients who were not solid organ donors,patients who were not declared brain dead(donation after circulatory death),and patients who did not receive steroids in their hospital *** and steroid administration,the number of organs donated,the types of organs donated,and demographic information were *** analyses were performed with Pred to be statistically *** A total of 88 patients met inclusion criteria,69(78%)of whom received levothyroxine and steroids(ST/LT group)vs 19(22%)receiving steroids without levothyroxine(ST group).No differences were observed between the groups for gender,race,pertinent injury factors,age,or other hormone therapies used(P>0.05).In the ST/LT group,68.1%(n=47)donated a high yield(3-5)of organ types per donor compared to 42.1%(n=8)in the ST group(P=0.038).There was no difference in the total number of organ types donated between the groups(P=0.068).CONCLUSION This study suggests that combining levothyroxine and steroid administration increases high-yield organ donation per donor in BPODs in the trauma patient *** to this study include

关键词： Organ donation Trauma Brain death Levothyroxine Hormone replacement therapy Steroids Organ donor retrospective

Enhancement of THz generation in LiNbO_(3) waveguides via multi-bounce velocity matching

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Light(Science & Applications) 2022年第12期11卷 2994-3001页

作者： blake S.Dastrup Eric r.Sung Frank Wulf Clara Saraceno Keith A.Nelson Department of Chemistry Massachusetts Institute of TechnologyCambridgeMA 02139USA Faculty of Electrical Engineering and Information Technology Ruhr University BochumBochum 44801Germany

To realize the full promise of terahertz polaritonics(waveguide-based terahertz field generation,interaction,and readout)as a viable spectroscopy platform,much stronger terahertz fields are needed to enable nonlinear and even robust linear terahertz *** use a novel geometric approach in which the optical pump is totally internally reflected to increase the distance over which optical rectification *** matching is achieved by tuning the angle of internal *** doing this,we are able to enhance terahertz spectral amplitude by over 10x compared to conventional single-pass terahertz *** analysis of the depletion mechanisms reveals that 3-photon absorption and divergence of the pump beam are the primary limiters of further *** level of enhancement is promising for enabling routine spectroscopic measurements in an integrated fashion and is made more encouraging by the prospect of further enhancement by using longer pump wavelengths.

关键词： pump waveguide tuning