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Gene Expression Profiling of Human Myeloid Leukemic MV4-11 Cells Treated with 5-Aza-2’-deoxycytidine

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j.urnal of Cancer Therapy 2012年第3期3卷 177-182页

作者： Kyu-Tae Kim David Mossman donald Small Rodney j. Scott Division of Genetics Hunter Area Pathology Service John Hunter Hospital New Lambton Australia School of Biomedical Sciences and Pharmacy Faculty of Health University of Newcastle Callaghan Australia School of Medicine Johns Hopkins University Baltimore USA

The pyrimidine analog, 5-aza-2’-deoxycytidine (5-aza-dC) is a DNA methyltransferase inhibitor that triggers DNA demethylation leading to the reactivation of epigenetically silenced tumor suppressor genes. To understand the shift in gene expression which mediates the beneficial 5-aza-dC effects in leukemia, we have treated human myeloid derived leukemic cells with 5-aza-dC. Target genes were identified first in MV4-11 cells using a genome-wide gene expression profiling assay to detect differences in treated and untreated cells. From this analysis six genes were identified (HOXA4, HOXD4, HOXA8, HOXD12, CD9 and RGS2) as being significantly different expressed after treatment. To validate microarray data, we performed quantitative PCR on these genes from multiple leukemic cells. The results suggest that these genes are epigenetically regulated indicating that dysregulation of HOXA4, HOXD4, HOXA8, HOXD12, CD9 and RGS2 expression may play an important role in establishing the malignant phenotype in AML.

关键词： 5-Aza-2’-deoxycytidine Gene Expression Profile HOX CD9 RGS2

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Phytochemistry, anti-asthmatic and antioxidant activities of Anchomanes difformis(Blume) Engl. leaf extract

作者：唐纳德·j.特朗普(donald j. Trump)

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Asian Pacific j.urnal of Tropical Biomedicine 2016年第3期6卷 225-231页

作者： Ovuakporie-Uvo Oghale Mac donald Idu Department of Plant Biology and Biotechnology University of Benin

Obj.ctive: To study the phytochemistry, anti-asthmatic and antioxidant activities of the aqueous leaf extract of Anchomanes difformis(Blume) Engl.(A. difformis) and to verify claims of use in folk ***: For anti-asthmatic activity, male and female guinea pigs with average body weight of(451.4 ± 118.1) g were divided into six groups of six animals each. Group 1served as control(distilled water); Group 2 was administered with salbutamol(reference drug) only; Group 3 served as ovalbumin sensitized group, Group 4, 5 and 6 were treated with A. difformis extract at doses of 100, 200 and 400 mg/kg, respectively. Described methods were used to test fluid viscosity, fluid volume and quantitative phytochemistry analysis. Absorbance was read using a UV–Vis spectrophotometer and results computed in percentage. Total antioxidant assays [2, 2-diphenyl-1-picrylhydrazyl(DPPH) and lipid peroxidation assay], were carried out using reported ***: The anti-asthmatic evaluation showed that protection from asthma of the animals in Group 6(400 mg/kg, 32.7%) were similar to that in Group 2(salbutamol, 33.0%).Excised trachea was free of mucus secretion in Group 5(200 mg/kg) as was observed in the control group. Fluid volume increase in Groups 3 and 6 indicated mucus *** radical scavenging activity of extract was effective as ascorbic acid which served as standard at 20 mg/m L. But, the extract elicited low lipid peroxidation activity compared with the reference(tocopherol) at concentrations ***: A. difformis aqueous leaf extract is safe and possesses positive antiasthmatic and antioxidant activities as claimed by traditional herbal practitioners in Delta State.

关键词： Anti-asthmatic Anti-oxidant Phytochemistry Anchomanes difformis

Src Is Dephosphorylated at Tyrosine 530 in Human Colon Carcinomas

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Chinese j.urnal of Cancer Research 2011年第3期23卷 229-231页

作者： j.ffrey D Bj.rge donald j.Fuj.ta Department of Biochemistry and Molecular Biology and Southern Alberta Cancer Research InstituteUniversity of Calgary

Obj.ctive：Src is a protein tyrosine kinase that plays important roles in cancer development,and Src kinase activity has been found to be elevated in several types of ***,the cause of the elevation of Src kinase activity in the maj.rity of human colon carcinomas is still largely *** aim at finding the cause of elevated Src kinase activity in human colon ***：We employed normal colon epithelial FHC cells and examined Src activation in human colon carcinoma specimens from 8 *** expression levels were determined by Western blotting,and the activity of Src kinase by kinase ***：Actin levels were different between tumor and normal tissues,demonstrating the complexities and inhomogeneities of the tissue *** kinase activities were increased in the maj.rity of the colon carcinomas as compared with normal colon epithelial cells （range 13-29）.Src protein levels were reduced in the colon *** Y530 phosphorylation levels were reduced to a higher extent than protein levels in the ***：The results suggest that Src specific activities were highly increased in human colon carcinomas;phosphorylation at Src Y530 was reduced,contributing to the highly elevated Src specific activity and Src kinase activity.

关键词： Src Kinase activation Dephosphorylation Colon carcinoma

A TAXONOMY OF j.VAN HOMINID MANDIBLES

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人类学学报 1992年第4期11卷 292-299页

作者： donald E.Tyler Idaho 83843 U.S.A Moscow Dept. of Anthropology University of Idaho

The hominid mandibular remains from the Pleistocene of j.va have been controversial since the discovery of Kedung Brubus (Mandible A) in 1890. Now, with several specimens, are they one species, or a mixture of species? What species of hominoid do these fossils represent?What was the fate of these hominoids? Were some replaced by a later hominid, or are they all representative of a single population? These mandibles are now called Kedung Brubus, and Sangiran 1 (Mandible B), 5 ("The Mandible of 1939") 6 (Meganthropus A), 8 (Meganthropus B),

关键词： In A TAXONOMY OF j.VAN HOMINID MANDIBLES than

Role of polyethylene particles in peri-prosthetic osteolysis:A review

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World j.urnal of Orthopedics 2011年第10期2卷 93-101页

作者： Gerald j.Atkins David R Haynes donald W Howie David M Findlay Discipline of Orthopaedics and Trauma The University of AdelaideSouth Australia 5005Australia Discipline of Pathology The University of AdelaideSouth Australia 5005Australia

There is convincing evidence that particles produced by the wear of j.int prostheses are causal in the periprosthetic loss of bone,or osteolysis,which,if it progresses,leads to the phenomenon of aseptic *** is important to fully understand the biology of this bone loss because it threatens prosthesis survival,and loosened implants can result in peri-prosthetic fracture,which is disastrous for the patient and presents a difficult surgical *** focus of this review is the bioactivity of polyethylene(PE)particles,since there is evidence that these are maj.r players in the development and progression of osteolysis around prostheses which use PE as the bearing *** review describes the biological consequences of interaction of PE particles with macrophages,osteoclasts and cells of the osteoblast lineage,including *** explores the possible cellular mechanisms of action of PE and seeks to use the findings to date to propose potential nonsurgical treatments for *** particular,a nonsurgical approach is likely to be applicable to implants containing newer,highly cross-linked PEs(HXLPEs),for which osteolysis seems to occur with much reduced PE wear compared with conventional *** caveat here is that we know little as yet about the bioactivity of HXLPE particles and addressing this constitutes our next challenge.

关键词： Polyethylene Aseptic loosening Osteolysis Wear particle Peri-prosthetic

Generalized Fourier Transform Method for Solving Nonlinear Anomalous Diffusion Equations

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Applied Mathematics 2019年第12期10卷 1039-1047页

作者： j.e Yao Cameron L. Williams Fazle Hussain donald j. Kouri Department of Mechanical Engineering Texas Tech University Lubbock TX USA Department of Mathematics University of Houston Houston TX USA Departments of Mechanical Engineering and Physics University of Houston Houston TX USA

The solution of a nonlinear diffusion equation is numerically investigated using the generalized Fourier transform method. This equation includes fractal dimensions and power-law dependence on the radial variable and on the diffusion function. The generalized Fourier transform approach is the extension of the Fourier transform method used for the normal diffusion equation. The feasibility of the approach is validated by comparing the numerical result with the exact solution for a point-source. The merit of the numerical method is that it provides a way to calculate anomalous diffusion with an arbitrary initial condition.

关键词： Generalized Fourier Transform Anomalous Diffusion Nonlinear

Calibration of the DEM sliding friction and rolling friction parameters of a cohesionless bulk material

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Particuology 2024年第9期92卷 126-139页

作者： j.natan Marín Pérez Tugrul Comlekci Yevgen Gorash donald MacKenzie University of Strathclyde 75 Montrose StreetG11XJGlasgowUnited Kingdom

The discrete element method(DEM)has become a valuable tool for understanding the mechanical behaviour of granular assemblies,however,the accuracy of the DEM simulations depends on several interaction parameters such as the sliding friction and rolling friction ***,these parameters are estimated using bulk calibration approach(BCA)where the draw down test has been suggested as an effective way to approach these *** test provides up to four bulk criteria,the angle of repose,shear angle,mass flow rate and the mass loss which are used to narrow down the possible ***,there is still more research needed around it to fully understand how this methodology *** experimental and numerical study was carried out using the draw down test to assess the influence of different mass flow rates and particle shapes on DEM parameters for a cohesionless bulk *** is concluded that use of multi-sphere particles and three aperture sizes in the draw down test to calibrate the sliding and rolling friction parameters of a cohesionless bulk material can converge to a small feasible region in which a single combination of the friction coefficients can be *** calibrated sliding and rolling friction coefficients were validated using multi-sphere particles,where the maximum deviation was 5.9%from the experimental values.

关键词： Discrete element method Calibration Draw down Sliding friction Rolling friction Particle shape