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Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples

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Infectious Diseases of poverty 2023年第4期12卷 108-108页

作者： Oyime p.Aula donald p.mcmanus Malcolm K.Jones Hong You pengfei Cai Catherine A.Gordon Molecular parasitology Laboratory QIMR Berghofer Medical Research Institute Brisbane Australia School of public Health Faculty of Medicine The University of Queensland Brisbane Australia School of Veterinary Science The University of Queensland Brisbane Australia Faculty of Medicine The University of Queensland Brisbane Australia

BackgroundSchistosomiasis remains a public health issue and the need for accurate and affordable diagnostics is crucial in the elimination of the disease. While molecular diagnostics are highly effective, they are expensive, with the main costs been associated with DNA extraction. The DNA dipstick is a rapid, affordable and simple purification method that allows DNA to be extracted from diagnostic samples within 30 s. We aimed to optimise the DNA dipstick method for samples from mice and egg-spiked human ***, blood and faeces were collected from mice exposed to Schistosoma japonicum infection at weekly intervals from Day 0 to Day 42. Urine and faecal samples were also collected from volunteer, uninfected humans and spiked with S. japonicum eggs. All samples were subject to several optimisation procedures and DNA extracted with the DNA dipstick. Amplification of the target DNA was carried out using LAMp and visualised using agarose gel electrophoresis and *** DNA dipstick successfully identified S. japonicum from infected mice and human clinical samples spiked with cracked eggs or genomic DNA from S. japonicum. Amplification was observed from week 4 post infection in infected mice. For human samples, amplification was observed in sieved faecal samples, filtered urine samples heated at 95 ℃ for 30 min, and sera samples heated at 95℃ for 30 *** DNA dipstick combined with LAMp has huge potential in providing cost-effective, simple and accurate detection of schistosomiasis infection in endemic regions. This will allow for rapid treatment, tracking outbreaks—such as occur after typhoons, leading to better health outcomes and contributing to control and eventual elimination of schistosomiasis.

关键词： Schistosomiasis Schistosoma japonicum DNA dipstick Loop-mediated isothermal amplification assay Flocculation

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The landscape epidemiology of echinococcoses

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Infectious Diseases of poverty 2016年第1期5卷 107-119页

作者： Angela M.Cadavid Restrepo Yu Rong Yang donald p.mcmanus Darren J.Gray patrick Giraudoux Tamsin S.Barnes Gail M.Williams Ricardo J.Soares Magalhães Nicholas A.S.Hamm Archie C.A.Clements Research School of population Health The Australian National UniversityCanberraNew South WalesAustralia Ningxia Medical University YinchuanNingxia Hui Autonomous RegionP.R.China Molecular parasitology Laboratory QIMR Berghofer Medical Research InstituteBrisbaneQueenslandAustralia Chrono-environment lab UMR6249University of Bourgogne Franche-Comté/CNRSBesançonFrance Institut Universitaire de France ParisFrance The University of Queensland School of Veterinary ScienceGattonQueenslandAustralia The University of Queensland Queensland Alliance for Agriculture and Food InnovationGattonQueenslandAustralia School of public Health The University of QueenslandBrisbaneQueenslandAustralia Child Health Research Centre The University of QueenslandBrisbaneQueenslandAustralia Faculty of Geo-Information Science and Earth Observation(ITC) University of TwenteEnschedeThe Netherlands

Echinococcoses are parasitic diseases of major public health importance *** infection results in chronic disease with poor prognosis and serious medical,social and economic consequences for vulnerable *** to recent estimates,the geographical distribution of Echinococcus *** is expanding and becoming an emerging and re-emerging problem in several regions of the *** endemicity is geographically heterogeneous and over time it may be affected by global environmental ***,landscape epidemiology offers a unique opportunity to quantify and predict the ecological risk of infection at multiple spatial and temporal ***,we review the most relevant environmental sources of spatial variation in human echinococcosis risk,and describe the potential applications of landscape epidemiological studies to characterise the current patterns of parasite transmission across natural and human-altered *** advocate future work promoting the use of this approach as a support tool for decision-making that facilitates the design,implementation and monitoring of spatially targeted interventions to reduce the burden of human echinococcoses in disease-endemic areas.

关键词： Landscape epidemiology Helminth infection Human echinococcosis Echinococcus spp Environmental change Geographic information systems Remote sensing Geostatistics

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performance of the point-of-care circulating cathodic antigen test in the diagnosis of schistosomiasis japonica in a human cohort from Northern Samar, the philippines

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Infectious Diseases of poverty 2021年第5期10卷 40-51页

作者： pengfei Cai Yi Mu Kosala G.Weerakoon Remigio M.Olveda Allen G.Ross donald p.mcmanus Molecular parasitology Laboratory Infectious Diseases ProgramQIMR Berghofer Medical Research InstituteBrisbaneAustralia Department of Health Research Institute for Tropical MedicineManilaPhilippines icddr bDhakaBangladesh present Address:Department of parasitology Faculty of Medicine and Allied SciencesRajarata University of Sri LankaSaliyapuraSri Lanka

Background:Zoonotic schistosomiasis,caused by Schistosoma japonicum,remains a major public health problem in the *** study aimed to evaluate the commercially available rapid diagnostic point-of-care circulating cathodic antigen(pOC-CCA)test in detecting individuals infected with *** in a human cohort from an endemic area for schistosomiasis japonica in the ***:Clinical samples were collectedin 18 barangays endemic for *** infection in Laoang and palapag municipalities,Northern Samar,the philippines,in *** presence of CCA in flter-concentrated urine samples(n=412)was evaluated using the commercial kits and the results were converted to images,which were further analyzed by ImageJ software to calculate R *** diagnostic performance of the immunochromatographic pOCCCA test was compared using the Kato-Katz(KK)procedure,in-house enzyme-linked immunosorbent assays(ELISAs)and droplet digital(dd)pCR assays as ***:The pOC-CCA test was able to detect ***-infected individuals in the cohort with an eggs per gram of faeces(EpG)more than or equal to 10 with sensitivity/specifcity values of 63.3%/93.3%.However,the assay showed an inability to diagnose schistosomiasis japonica infections in all cohort KK-positive individuals,of which the majority had an extremely low egg burden(EpG:1–9).The prevalence of *** infection in the total cohort determined by the pOC-CCA test was 12.4%,only half of that determined by the KK method(26.2%).When compared with the ELISAs and ddpCR assays as a reference,the pOC-CCA assay was further shown to be a test with low ***,the assay exhibited signifcant positive correlations with egg burden determined by the KK technique and the target gene copy number index values determined by the ddpCR assays within the entire ***:By using in silico image analysis,the pOC-CCA cassette test could be converted to a quantitative assay to avoid reader

关键词： Schistosomiasis Schistosoma japonicum Kato-Katz pOC-CCA ELISA Droplet digital pCR

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Health-education to prevent COVID-19 in schoolchildren: a call to action

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Infectious Diseases of poverty 2020年第4期9卷 142-144页

作者： Darren J.Gray Johanna Kurscheid Mary Lorraine Mationg Gail M.Williams Catherine Gordon Matthew Kelly Kinley Wangdi donald p.mcmanus Department of Global Health Research School of Population HealthAustralian National UniversityCanberraAustralia School of public Health University of QueenslandBrisbaneAustralia Infectious Diseases Division QIMR Berghofer Medical Research InstituteBrisbaneAustralia

Background:There is currently considerable international debate around school closures/openings and the role of children in the transmission of coronavirus disease 2019(COVID-19).Whilst evidence suggests that children are not impacted by COVID-19 as severely as adults,little is still known about their transmission potential,and with a lot of asymptomatic cases they may be silent transmitters(*** without showing clinical signs of disease),albeit at a lower level than *** relation to this,it is somewhat concerning that in many countries children are cared for,or are often in close contact with,older individuals such as grandparents—the age group most at risk of acquiring serious respiratory complications resulting in *** text:We emphasise that in the absence of a vaccine or an effective therapeutic drug,preventive measures such as good hygiene practices—hand washing,cough etiquette,disinfection of surfaces and social distancing represent the major(in fact only)weapons that we have against ***,we stress that there is a pressing need to develop specific COVID-19 prevention messages for ***:An entertainment education intervention for schoolchildren systematically implemented in schools would be highly effective and fill this *** such measures in place there would be greater confidence around the opening of schools.

关键词： COVID-19 Children Health education Health promotion prevention

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Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis

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Infectious Diseases of poverty 2018年第1期7卷 761-769页

作者： Cong-Nuan Liu Yang-Yang Xu Angela M.Cadavid-Restrepo Zhong-Zi Lou Hong-Bin Yan Li Li Bao-Quan Fu Darren J.Gray Archie A.Clements Tamsin S.Barnes Gail M.Williams Wan-Zhong Jia donald p.mcmanus Yu-Rong Yang State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary parasitology of Gansu province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute CAASLanzhou 730046People’s Republic of China The Human pathology and Immunology Department Ningxia Medical UniversityYinchuanNingxia Hui Autonomous RegionPeople’s Republic of China Neurosurgery Department Tianjin Xiqing HospitalTianjinPeople’s Republic of China Molecular parasitology Laboratory QIMR Berghofer Medical Research InstituteBrisbaneAustralia Research School of population Health Australian National UniversityCanberraAustralia School of population Health Infectious Disease Epidemiology UnitUniversity of QueenslandBrisbaneAustralia Queensland Alliance for Agriculture and Food Innovation University of QueenslandGattonAustralia

Background:Cystic echinococcosis(CE)and alveolar echinococcosis(AE)are highly endemic in Xiji County of Ningxia Hui Autonomous Region(NHAR)in China where the control campaign based on dog de-worming with praziquantel has been undertaken over preceding *** study is to determine the current prevalence of Echinococcus granulosus and *** in domestic dogs and monitor the echinococcosis transmission ***:Study villages were selected using landscape patterns(Geographic Information System,GIS)for Echinococcus transmission“hot spots”,combined with hospital records identifying risk areas for AE and CE.A survey of 750 domestic dogs,including copro-sampling and owner questionnaires,from 25 selected villages,was undertaken in 2012.A copro-multiplex pCR assay was used for the specific diagnosis of *** and *** in the *** analysis,using IBM SpSS Statistics,was undertaken,to compare the prevalence of the two Echinococcus *** dogs between four geographical areas of Xiji by theχ^(2)*** analysis of the combinations of outcomes from the questionnaire and copro-pCR assay data was carried out to determine the significant risk factors for dog ***:The highest de-worming rate of 84.0%was found in the northwest area of Xiji County,and significant differences(pphical areas of Xiji were *** highest prevalence(19.7%,59/300)of *** occurred in northwest Xiji,though the highest prevalence(18.1%,38/210)of *** occurred in southwest *** was no significant difference(p>0.05)in the prevalence of *** in dogs from the northwest,southwest,northeast,and southeast of Xiji,but there were significant differences(ppendent variables was statistically ***:The results from this study indicate a high prevalenc

关键词： Domestic dog surveys Dog-copro-multiplex pCR assay Co-endemicity of Echinococcus granulosus and E.multilocularis Xiji County Ningxia hui autonomous region(NHAR) p.R.China

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Real-time pCR diagnosis of Schistosoma japonicum in low transmission areas of China

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Infectious Diseases of poverty 2018年第1期7卷 87-97页

作者： pei He Catherine A.Gordon Gail M.Williams Yuesheng Li Yuanyuan Wang Junjian Hu Darren J.Gray Allen G.Ross donald Harn donald p.mcmanus Hunan Institute of parasitic Diseases YueyangPeople’s Republic of China Molecular parasitology Laboratory Infectious Diseases DivisionQIMR Berghofer Medical Research InstituteBrisbaneAustralia Discipline of Epidemiology and Biostatistics School of Population HealthUniversity of QueenslandBrisbaneAustralia Research School of population Health The Australian National UniversityCanberraAustralia Menzies Health Institute Queensland Griffith UniversityGold CoastQLDAustralia University of Georgia College of Veterinary MedicineAthensGAUSA.

Background:Schistosomiasis in the people’s Republic of China(pRC)can be traced back to *** the past 60 years,the Chinese government has made great efforts to control this persistent disease with elimination slated by 2020 through the implementation of a comprehensive control *** strategy aims to reduce the role of bovines and humans as sources of infection as a pre-requisite for elimination through transmission *** goal of elimination will be achievable only by the implementation of a sustainable surveillance and control system,with sensitive diagnosis a key feature so that the true disease burden is not *** used diagnostics lack the necessary sensitivity to accurately determine the prevalence of Schistosoma japonicum infection in areas with low infection *** is of critical importance to find and treat people and to identify animals with low-level infections if the National Control programme for China is to achieve schistosomiasis ***:We evaluated a real-time polymerase chain reaction(qpCR)assay using 633 human stool samples collected from five villages in Hunan,Anhui,Hubei,and Jiangxi provinces,and 182 bovine(70 cattle and 112 buffalo)stool samples obtained from four villages in Hunan,Anhui,and Jiangxi provinces in the *** stool samples were subjected to the miracidium hatching test(MHT,a diagnostic procedure used in the National Schistosomiasis Control programme)and the qpCR *** positive by MHT were subjected to either the Kato-Katz technique for humans,or the formalin-ethyl acetate sedimentation-digestion(FEA-SD)procedure for bovines,to determine infection ***:The qpCR assay exhibited a high level of sensitivity in the detection of *** *** both the human and bovine samples,a significantly higher prevalence was determined using the qpCR assay(11.06%humans,24.73%bovines)than with the MHT(0.93%humans,7.69%bovines).The animal contamination

关键词： Schistosomiasis Schistosoma Japonicum China Real-time pCR Miracidium hatching test Kato-Katz FEA-SD Bovine Human

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The genome and transcriptome of the hydatid tapeworm Echinococcus granulosus

The genome and transcriptome of the hydatid tapeworm Echinoc...

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第四届全国人畜共患病学术研讨会 2014年中国狂犬病年会中国畜牧兽医学会兽医公共卫生学分会第四次学术研讨会

作者： Wenbao Zhang Zhuangzhi Zhang Huajun Zhen Jun Li Hui Wang Baoxin Shi Yan Zhou Shengyue Wang donald p.mcmanus Hao Wen Clinical Medical Research Institute The First Affiliated Hospital of Xinjiang Medical University Veterinary Research Institute Xinjiang Academy of Animal Sciences Shanghai–Ministry of Science and Technology Key Laboratory of Health and Disease Genomics Chinese National Human Genome Center at Shanghai

Cystic echinococcosis (hydatid disease),caused by the tapeworm Echinococcus granulosus,is responsible for considerable human morbidity and *** cosmopolitan disease is difficult to diagnose,treat and *** sequenced the whole genomic sequence for the worm comprising 151.6 Mb encoding 11,325 *** with the genome sequences from other taxa show that *** has acquired a spectrum of genes,including the EgAgB family,whose products are secreted by the parasite to interact and redirect host immune *** also found that genes in bile salt pathways may control the bidirectional development of E

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The control of soil-transmitted helminthiases in the philippines: the story continues

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Infectious Diseases of poverty 2021年第3期10卷 98-98页

作者： Mary Lorraine S.Mationg Veronica L.Tallo Gail M.Williams Catherine A.Gordon Archie C.A.Clements donald p.mcmanus Darren J.Gray Department of Global Health Research School of Population HealthThe Australian National UniversityBuilding 62 Mills RdActon ACTCanberra 2601Australia 不详

Background:Soil-transmitted helminth(STH)infections have long been an important public health concern in the *** this review,we describe the current status of STH infections there and highlight the control efforts undertaken to reduce STH *** text:A nationwide STH mass drug administration(MDA)programme was started in 2006 but the overall STH prevalence remains stubbornly high across the philippines,rangi ng from 24.9%to 97.4%.

关键词： Soil-transmitted helminths Epidemiology Control The philippines

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口服蒿甲醚控制重疫区血吸虫病及预防急性感染

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中国血吸虫病防治杂志 2006年第1期18卷 32-35页

作者：陈红根林丹丹李岳生刘跃民 donald p.mcmanus 黄兴华冯正江西省寄生虫病防治研究所南昌330046 湖南省血吸虫病防治研究所 The QIMR University of Queensland 江西省余干县血吸虫病防治站中国疾病预防控制中心寄生虫病预防控制所

目的观察口服蒿甲醚控制日本血吸虫病重疫区人群感染及预防急性感染的效果。方法在鄱阳湖区血吸虫病易感季节（5～10月）对重疫区村居民采用15d间隔口服蒿甲醚预防感染。同时设对照组，口服安慰剂。结果感染季节结束后，实验组人群... 详细信息

目的观察口服蒿甲醚控制日本血吸虫病重疫区人群感染及预防急性感染的效果。方法在鄱阳湖区血吸虫病易感季节（5～10月）对重疫区村居民采用15d间隔口服蒿甲醚预防感染。同时设对照组，口服安慰剂。结果感染季节结束后，实验组人群粪检阳性率为0．83％，无急性感染发生；对照组粪检阳性率为15．01％，发生3例急性感染。结论每15天间隔口服蒿甲醚1次，对重疫区人群有良好的保护作用，保护率为94．47％，有利于控制人群感染；对防止急性感染也有明显效果。

关键词：蒿甲醚预防急性血吸虫病

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VDR-Taq Ⅰ、Fok Ⅰ位点多态性与宁夏人群结核易感性研究

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中国防痨杂志 2013年第8期35卷 566-572页

作者：陈丹丹朱圭娜关光玉 Magda Ellis donald p. mcmanus 杨玉荣宁夏医科大学病原生物学与免疫学系银川研究生750004 宁夏疾病预防控制中心研究所澳大利亚新南威尔士州结核研究所澳大利亚昆士兰医学研究所传染病研究部

目的探讨维生素D受体(VDR)基因TaqⅠ、FokⅠ位点基因多态性与宁夏回族自治区人群肺结核易患性之间的关系。方法搜集宁夏南部8个地区2010年7—11月确诊的肺结核患者993例(简称"病例组"),其中男550例,女443例;同期选择来源地区,与病例组... 详细信息

目的探讨维生素D受体(VDR)基因TaqⅠ、FokⅠ位点基因多态性与宁夏回族自治区人群肺结核易患性之间的关系。方法搜集宁夏南部8个地区2010年7—11月确诊的肺结核患者993例(简称"病例组"),其中男550例,女443例;同期选择来源地区,与病例组性别相同、民族相同、年龄相当、居住环境相匹配的确认无肺结核的健康人880名(简称"对照组"),其中男485名,女395名。将聚合酶链反应-限制性片段长度多态性(pCR-RFLp)检测VDR基因多态性的方法,应用于人群结核病易感性的病例-对照研究。用SpSS 16.0软件对检测结果进行数据统计,各基因型与结核易感性关系用单因素分析和多因素logistic回归分析,以pp=0.046)。VDR-FF、Ff、ff基因型病例组和对照组分布频率分别为32.4%(316/976)、47.9%(468/976)、19.7%(192/976)和28.5%(245/861)、53.3%(459/861)、18.2%(157/861),病例组和对照组分布差异无统计学意义(χ2=5.41,p=0.067)。在显性基因模型中,VDR-(TT+Tt)、tt基因型病例组和对照组中分布频率分别为98.2%(964/982)、1.8%(18/982)和99.4%(867/872)、0.6%(5/872),病例组和对照组中分布差异有统计学意义(χ2=5.98,p=0.014;OR=0.98、3.19,95%CI=0.978～0.997、1.193～8.574);在纯合基因模型中VDR-(FF+ff)、Ff基因型在病例组和对照组中频率分别为52.0%(508/976)、48.0%(468/976)和46.7%(402/861)、53.3%(459/861),病例组和对照组分布差异有统计学意义(χ2=5.27,p=0.022;OR=1.12、0.89,95%CI=1.023～1.298、0.822～0.985)。结论在宁夏人群中,肺结核易患性可能与VDR-TaqⅠ、FokⅠ位点多态性有关联,VDR-(FF+ff)、tt基因型可能是罹患肺结核的危险因素,而VDR-(TT+Tt)、Ff基因型可能是保护因素。

关键词：结核,肺遗传学受体,骨化三醇多态性,单核苷酸疾病遗传易感性病例对照研究宁夏[回族自治区]

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