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N-terminal truncated RHT-1 proteins generated by translational reinitiation cause semi-dwarfing of wheat Green Revolution alleles

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Molecular Plant 2021年第4期14卷 679-687页

作者： Karel Van De Velde Stephen GThomas Floor Heyse Rim Kaspar dominique Van Der Straeten Antje Rohde BASF Belgium coordination center-Innovation center Gent Technologiepark 101.9052 GhentBelgium Rothamsted Research HarpendenHertfordshire AL52JQUK Laboratory of Functional Plant Biology Department of BiologyGhent UniversitK.L.Ledeganckstrat 359000 GhentBelgium

The unprecedented wheat yield increases during the Green Revolution were achieved through the introduc-tion of the Reduced height(Rht)-B1b and Rht-D1b semi-dwarfing *** Rht-1 alleles encode growth-repressing DELLA genes containing a stop codon within their open reading frame that confers gibberellin(GA)-insensitive *** this study,we successfully took the hurdle of detecting wild-type RHT-1 proteins in different wheat organs and confirmed their degradation in response to *** further demonstrated that Rht-B1b and Rht-D1b produce N-terminal truncated proteins through trans-lational *** of these N-terminal truncated proteins in transgenic lines and in Rht-D1c,an allele containing multiple Rht-D1b copies,demonstrated their ability to cause strong dwarfism,resulting from their insensitivity to GA-mediated degradation.N-terminal truncated proteins were detected in spikes and nodes,but not in the aleurone *** Rht-B 1b and Rht-D1b alleles cause dwarfism but have wild-type dormancy,this finding suggests that tissue-specific differences in translational reinitiation may explain why the Rht-1 alleles reduce plant height without affecting *** together,our findings not only reveal the molecular mechanism underlying the Green Revolution but also demonstrate that trans-lational reinitiation in the main open reading frame occurs in plants.

关键词： gibberellin(GA)signaling DELLA Rht-1 dwarfing ales Green Revolution wheat translational reinitiation

Improvement of Fermented Fish Flour Quality Using Essential Oil Extracted From Fresh Leaves of Pimenta racemosa(Mill.)***

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Natural Products and Bioprospecting 2017年第4期7卷 299-305页

作者： Euloge S.Adjou Rene G.Degnon Edwige Dahouenon-Ahoussi Mohamed M.Soumanou dominique c.K.Sohounhloue Laboratory of Research and Study in Applied chemistry Polytechnic School of Abomey-CalaviUniversity of AbomeyCalavi01P.O.B:2009CotonouBenin

The aim of this study was to evaluate the efficacy of the essential oil extracted from fresh leaves of Pimenta racemosa in the improvement of fermented fish flour producing *** oil of Pimenta racemosa was extracted by hydrodistillation and its chemical composition was determined by Gc and Gc/*** types of fermented fish flours from Lesser African Threadfin(Galeoides decadactylus)were produced by the modification of the traditional processing technology and the introduction of a step of essential oil adjunction during the *** different essential oil concentrations(0.5,1.0 and 2.0 lL g^(-1))were ***,microbiological and nutritional analyzes were performed in order to evaluate the quality of the fermented fish flour *** obtained revealed that the essential oil of Pimenta racemosa investigated has a chemical composition characterized by the presence of myrcene(25.1%),chavicol(7.5%)and eugenol(51.1%).Fermented fish flour produced have a good nutritional ***,on the microbiological level,only samples produced by adjunction of essential oil have a low level of microbial contamination,with an absence of pathogenic microorganisms.

关键词： Essential oil Pimenta racemosa Galeoides decadactylus Fermented fish Process Benin

Impact of ribavirin dose on retreatment of chronic hepatitis c patients

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World Journal of Gastroenterology 2012年第23期18卷 2966-2972页

作者： christiane Stern Michelle Martinot-Peignoux Marie Pierre Ripault Nathalie Boyer corinne castelnau dominique Valla Patrick Marcellin Service d'Hépatologie and INSERM U773-cRB3 H pital Beaujon University Paris-Diderot

AIM: To study the efficacy and factors associated with a sustained virological response (SVR) in chronic hepatitis c (cHc) relapsing patients. METHODS: Out of 1228 cHc patients treated with pegylated interferon (PEG-IFN) and ribavirin (RBV), 165 (13%) had a relapse. Among these, 62 patients were retreated with PEG-IFN-2a or-2b and RBV. clinical, biological, virological and histological data were collected. Initial doses and treatment modifications were recorded. The efficacy of retreatment and predictive factors for SVR were analyzed. RESULTS: An SVR was achieved in 42% of patients. SVR was higher in young (course was associated with higher SVR rates (53% vs 28%, P = 0.04). Also, a better SVR rate was observed with RBV dose/body weight > 15.2 mg/kg per day (70% vs 35%, P = 0.04). In logistic regression, predictors of a response were age (P = 0.018), genotype (P = 0.048) and initial RBV dose/body weight (P = 0.022). None of the patients without a complete early virological response achieved an SVR (negative predictive value = 100%). cONcLUSION: Retreatment with PEG-IFN/RBV is effective in genotype 2 or 3 relapsers, especially in young patients. A high dose of RBV seems to be important for the retreatment response.

关键词： chronic hepatitis c Relapse Retreatment Ribavirin Pegylated interferon

The role of photovaporization of the prostate in small volume benign prostatic hyperplasia and review of the literature

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Asian Journal of Urology 2019年第4期6卷 353-358页

作者： dominique Thomas Kevin c.Zorn Malek Meskawi Ramy Goueli Pierre-Alain Hueber Lesa Deonarine Vincent Misrai Alexis Te Bilal chughtai Department of Urology Weill Cornell Medical College/New York PresbyterianNew YorkNYUSA Section of Urology Department of SurgeryUniversity of Montreal Hospital CenterMontrealQCCanada

Objective:Our objective was to characterize the safety and efficacy of the 180 W XPS-GreenLight laser in men with lower urinary tract symptoms secondary to a small volume benign prostatic hyperplasia(BPH).Methods:A retrospective analysis was performed for all patients who underwent 180 W XPSlaser photoselective vaporization of the prostate(PVP)vaporization of the prostate between 2012 and 2016 at two-tertiary medical *** collection included baseline comorbidities,disease-specific quality of life scores,maximum urinary flow rate(Qmax),postvoid residual(PVR),complications,prostate volume and prostate-specific antigen(PSA).The secondary endpoints were the incidence of intraoperative and postoperative adverse *** were stratified using the clavien-Dindo grading system up to 90 days after ***:Mean age of men was 67.8 years old,with a mean body mass index of 29.7 kg/*** prostate volume as measured by transrectal ultrasound was 29 *** use was 47%and urinary retention with catheter at time of surgery was 17%.Mean hospital stay and catheter time were 0.5 *** follow-up time was 6 months with the longest duration of follow-up being 22.5 months(interquartile range,3-22.5 months).The International Prostate Symptom Score improved from 22.8±7.0 at baseline to 10.7±7.4(pctively,while the PVR improved from 216.0±271.0 mL preoperatively to 32.8±45.3 mL(pck contracture postoperatively and no capsular perforations were noted ***:The 180 W GreenLight XPS system is safe and effective for men with small volume

关键词： Benign prostatic hyperplasia Photovaporization of the prostate GreenLight XPS Lower urinary tract symptoms Small prostate

c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear cyclin D1

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World Journal of Gastroenterology 2008年第41期14卷 6339-6346页

作者： Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel dominique Bernuau Fanny Daniel Institut National de laSanté et de la Recherche Médicale U697 Hpital Saint-Louis Institut National de la Santé et de la RechercheMédicale U591 Faculté de Médecine Necker Institut National de la Santé et de la RechercheMédicale U773 Centre de Recherche Bichat Beaujon CRB3and Université Paris 7 Denis Diderot site Bichat

AIM： To investigate the effect of stable c-Fos over- expression on immortalized human hepatocyte （IHH） proliferation. METHODS： IHHs stably transfected with c-Fos （IHH- Fos） or an empty vector （IHH-c） were grown in me- dium supplemented with 1% serum or stimulated with 10% serum. cell proliferation was assessed by cell counts, 3H-thymidine uptake and flow cytometry analyses. The levels of cell cycle regulatory proteins （cyclin DI, E, A） cyclin dependent kinases （cdk） cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, total and phosphorylated GSK-3β and epidermal growth factor receptor （EGF-R） were assayed by Western blotting. Analysis of O/c/in D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction （PcR） analysis. Stability of cyclin DI was studied by cycloheximide blockade experiments. RESULTS： Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. cell cycle analysis by flow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, cyclin D1 was more abundantly expressed in c-Fos overexpress- ing cells. cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein com- pared to IHH-c both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specific EGF-R tyro- sine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased cyclin D1 stability in the nucleus. cONcLUSION： Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate

关键词： c-Fos cyclin DI GSK-3 cell growth, cell cycle Hepatoma Epidermal growth factor

中法两家医院早发型新生儿脓毒症临床资料对比分析

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中华儿科杂志 2012年第9期50卷 664-671页

作者：范颖余加林 dominique ASTRUc 重庆医科大学附属儿童医院新生儿科 400014 67098 Strasbourg cedex Department ofNeonatology Hautepierre Hospital of Strasbourg University Strasbourg France

目的了解中法两国对早发型新生儿脓毒症（early—onsetneonatalsepsis，EONS）在临床表现、诊断、治疗方面的不同，为提高重庆医科大学附属儿童医院（简称重医）对EONS的诊治水平提供依据。方法对法国某医院和重医共146例EONS患儿的细... 详细信息

目的了解中法两国对早发型新生儿脓毒症（early—onsetneonatalsepsis，EONS）在临床表现、诊断、治疗方面的不同，为提高重庆医科大学附属儿童医院（简称重医）对EONS的诊治水平提供依据。方法对法国某医院和重医共146例EONS患儿的细菌谱、临床表现、抗菌药使用、产前危险因素记录和筛查情况进行回顾性分析。结果重医常见致病菌为凝固酶阴性葡萄球菌（69．2％）和大肠杆菌（15．4％）；法国医院为B族链球菌（33．3％）和大肠杆菌（33．3％）。法国医院胃液和周围拭子培养常见大肠杆菌（33．3％），B族链球菌（21．2％），凝固酶阴性葡萄球菌（18．2％）等。重医抗菌药使用总天数11．4±7．2（d）、平均每个患儿抗菌药使用的种类（3．1±0．9）和换抗菌药人数比例（70．2％）均大于法国医院6．2±2．5（d），2．2±0．8（d），（9．9％）。两院首剂抗菌药常联用两种抗菌药（重医二代头孢＋半合成青霉素，法国医院阿莫西林＋阿米卡星）。重医二、三线抗菌药常见碳青霉烯类和万古霉素，法国医院常见三代头孢和万古霉素。重医对多种产前危险因素（绒毛膜羊膜炎、母亲发热、破膜到胎儿娩出时间、孕期是否行阴道拭子或尿路感染情况、羊水污染、产前抗菌药使用、EONS的既往史）的记录率均低于法国医院；产前危险因素筛查阳性率两院差异不大。在生后立即出现异常而入院的患儿中，两院最常见的异常均为呼吸困难（96．5％VS．88．2％）；在生后72h之内出现异常的患儿中，两院常见入院原因构成比不同：重医常见原因为呼吸困难（44．4％），反应差（22．2％）等；法国医院常见原因为c反应蛋白升高（78．2％），发热（5．5％）等。两所医院cRP诊断EONS的假阴性率无统计学差异。结论两所医院对EONS诊断、治疗有很大不同。重视产前危险因素的筛查与记录、加强产科对初生新生儿的监护可提高重医对EONS患儿的早期诊断的准确性。对具有产前／产时危险因素的新生儿积极进行胃液／周围拭子培养并行相应的药敏试验可能帮助临床医生进行抗菌药的选用．减少患儿的换药次数和抗菌药使用时间。

关键词：婴儿新生感染脓毒症抗菌药

A de novo germline MLH1 mutation in a Lynch syndrome patient with discordant immunohistochemical and molecular biology test results

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World Journal of Gastroenterology 2012年第39期18卷 5635-5639页

作者： Fabrice Airaud Sébastien Küry Isabelle Valo Ingrid Maury dominique Bonneau Olivier Ingster Stéphane Bezieau cHU Nantes Service de Génétique Médicale9 quai Moncousu44093 Nantes Cedex 1France Institut de cancérologie de l'Ouest Service d'Anatomie et Cytologie Pathologique2 rue Moll49933 Angers Cedex 9France cHU Angers Service de Génétique Médicale4 rue Larrey49933 AngersFrance

We describe a patient with a Homo sapiens mutL homolog 1 (MLH1)-associated Lynch syndrome with previous diagnoses of two distinct primary cancers:a sigmoid colon cancer at the age of 39 years, and a right colon cancer at the age of 50 years. The mutation identified in his blood and buccal cells, c.1771delG, ***591Ilefs*25, appears to be a de novo event, as it was not transmitted by either of his parents. This type of de novo event is rare in MLH1 as only three cases have been reported in the literature so far. Further-more, the discordant results observed between repli-cation error phenotyping and immunohistochemistry highlight the importance of the systematic use of both pre-screening tests in the molecular diagnosis of Lynch syndrome.

关键词： Lynch syndrome Homo sapiens mutL ho-molog 1 De novo mutation Replication error pheno- type Immunohistochemistry

Evaluation of an Ultrasound-Guided Focused Ultrasound cMUT Probe for Targeted Therapy Applications

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Open Journal of Applied Sciences 2018年第1期8卷 25-45页

作者： dominique Gross Mathieu Legros Philippe Vince dominique certon VERMON SA Tours France Fran&ccedil ois-Rabelais University CETU Altha&iuml s Technologies Tours France GREMAN UMR 7347 Université de Tours CNRS INSA Centre Val de Loire Tours France

capacitive Micromachined Ultrasonic Transducer (cMUT) technology, which has been widely studied in the field of medical imaging, possesses strong design flexibility due to its manufacturing process. Many applications could benefit from this unique feature, especially those that require different operating ultrasonic frequencies. This article reports on the characterization of the therapeutic low-frequency field provided by an ultrasound-guided focused ultrasound cMUT probe that is connected to a custom ultrasonic scanner for hyperthermia applications. The study begins by mapping the focused ultrasonic beam in the vicinity of the focal spot and a parametric analysis providing the maximum peak-to-peak (PTP) pressure delivered by the probe under different acoustic conditions. The measured maximum PTP pressure at the targeted operating frequency of 1 MHz is 3 MPa, with a maximum of 3.6 MPa at 1.25 MHz. Based on an in vitro setup found in the literature, the temperature elevation at the focal point was measured, showing results in agreement with the targeted applications (max. ΔT = 7.5°c). The article concludes with a reliability study considering the delivered pressure and the self-heating of the cMUT probe: the results show the good stability of the pressure amplitude over 1.8 × 109 cycles at a duty cycle of 40%, with a moderate internal heating of 3°c.

关键词： capacitive Micromachined Ultrasonic Transducers cMUT USgFUS Targeted Therapy

René Lalique:joaillier et artiste verrier

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法语学习 2005年第3期 92-94页

作者： dominique Maitrot