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Global warming scenarios for the Eastern Adriatic Sea indicate a higher risk of invasiveness of non-native marine organisms relative to current climate conditions

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Marine life Science & Technology 2024年第1期6卷 143-154页

作者： Branko Glamuzina lorenzo Vilizzi Marina Piria Ante Zuljevic Ana Bratos Cetinic Ana Pesic Branko Dragicevic lovrenc lipej Marijana Pecarevic Vlasta Bartulovic Sanja Grđan Ivan Cvitkovic Tatjana Dobroslavic Ana Fortic luka Glamuzina Borut Mavric Jovana Tomanic Marija Despalatovic domen Trkov Marina Brailo Scepanovic Zoran Vidovic Predrag Simonovic Sanja Matic-Skoko Pero Tutman Department of Applied Ecology University of DubrovnikDubrovnikCroatia Department of Ecology and Vertebrate Zoology Faculty of Biology and Environmental ProtectionUniversity of LodzLodzPoland Faculty of Agriculture Department of FisheriesApicultureWildlife Management and Special ZoologyUniversity of ZagrebZagrebCroatia Institute of Oceanography and Fisheries SplitCroatia Institute of Marine Biology University of MontenegroKotorMontenegro National Institute of Biology Marine Biology Station PiranPiranSlovenia Teacher Education Faculty University of BelgradeBelgradeSerbia Faculty of Biology University of BelgradeBelgradeSerbia

Globally,marine bioinvasions threaten marine ecosystem structure and function,with the Mediterranean Sea being one of the most affected *** invasions are expected to increase due to climate *** conducted a risk screening of marine organisms(37 fishes,38 invertebrates,and 9 plants),both extant and‘horizon’(i.e.,not present in the area but likely to enter it).Based on expert knowledge for the Eastern Adriatic Sea coasts of Slovenia,Croatia,and Montenegro,screenings were conducted under both current and predicted climate conditions indicating with an increase in sea surface temperature and salinity of the Adriatic Sea together with changes in precipitation *** aims were to:(1)identify non-native extant and horizon marine species that may pose threats to native biodiversity and(2)evaluate the risk of invasiveness of the selected species under current and predicted climate *** the 84 species screened,there was an increase in those ranked as‘high risk’from 33(39.3%)under current climate conditions and to 47(56.0%)under global warming *** those ranked as‘very high’risk,the increase was from 6(7.1%)to 21(25.0%).Amongst the screened species,the already established high-risk species pacific oyster Magallana gigas and Atlantic blue crab Callinectes sapidus represent a threat to ecosystem *** the under-representation of marine species in the current European Union list,the species we have ranked as high to very high risk should be included.

关键词： AS-ISK Early detection Introduction vectors/pathways Invasive species Rapid response

Electrochemical inactivation of cyanobacteria and microcystin degradation using a boron-doped diamond anode-A potential tool for cyanobacterial bloom control

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Journal of Environmental Sciences 2017年第3期29卷 248-261页

作者： Andrej Meglic Anja Pecman Tinkara Rozina domen lestan Bojan Sedmak Arhel ltd. Pustourhoua c.63SI-1000 LjubljanaSlovenia Centre for Soil and Environmental Sciences Biotechnical FacultyUniversity of LjubljanaJamnikarjeva 101SI-1000 LjubljanaSlovenia Envit ltd. Vojkova c.63SI-1000 LjubljanaSlovenia Department of Genetic Toxicology and Cancer Biology National Institute of BiologyVecna pot 111SI-1000 LjubljanaSlovenia

Cyanobacterial blooms are global phenomena that can occur in calm and nutrient-rich （eutrophic） fresh and marine waters. Human exposure to cyanobacteria and their biologically active products is possible during water sports and various water activities, or by ingestion of contaminated water. Although the vast majority of harmful cyanobacterial products are confined to the interior of the cells, these are eventually released into the surrounding water following natural or artificially induced cell death. Electrochemical oxidation has been used here to damage cyanobacteria to halt their proliferation, and for microcystin degradation under in-vitro conditions. partially spent Jaworski growth medium with no addition of supporting electrolytes was used. Electrochemical treatment resulted in the cyanobacterial loss of cell-buoyancy regulation, cell proliferation arrest, and eventual cell death. Microcystin degradation was studied separately in two basic modes of treatment： batch-wise flow, and constant flow, for electrolytic-cell exposure. Batch-wise exposure simulates treatment under environmental conditions, while constant flow is more appropriate for the study of boron-doped diamond electrode efficacy under laboratory conditions. The effectiveness of microcystin degradation was established using high-performance liquid chromatography-photodiode array detector analysis, while the biological activities of the products were estimated using a colorimetric protein phosphatase-1 inhibition assay. The results indicate potential for the application of electro-oxidation methods for the control of bloom events by taking advantage of specific intrinsic ecological characteristics of bloom-forming cyanobacteria. The applicability of the use of boron-doped diamond electrodes in remediation of water exposed to cyanobacteria bloom events is discussed.

关键词： Cyanobacterial bloom controlBoron-doped diamond anodeElectro-oxidationMicrocystin degradationProtein phosphatase-1 inhibitionWater remediation

Synthesis and visible-light-induced sacrificial photocatalytic water oxidation of quinary oxynitride BaNb_(0.5)Ta_(0.5)O_2N crystals

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Journal of Energy Chemistry 2018年第5期27卷 1415-1421页

作者： Kenta Kawashima Mirabbos Hojamberdiev Kunio Yubuta Kazunari domen Katsuya Teshima Department of Chemistry The University of Texas at Austin Department of Materials Physics Nagoya University Institute for Materials Research Tohoku University Department of Chemical System Engineering School of Engineering The University of Tokyo Department of Environmental Science and Technology Faculty of Engineering Shinshu University Center for Energy and Environmental Science Shinshu University

Quinary oxynitride BaNb_0.5Ta_0.5O_2N crystals were fabricated through the partial nitridation and acidifica- tion of the KCI flux grown Ba_5Nb_2Ta_2O_15 crystals. The parameters of both the solute concentrations and cooling rates are optimized for the KCI flux growth of the larger Ba_5Nb_2Ta_2O_15 crystals with clearer crystal habits. Here, the optimal Ba_5Nb_2Ta_2O_15 crystals mainly have a hexagonal plate-like shape. After the par- tial nitridation and acidification, the porous BaNb_0.5Ta_0.5O_2N crystals maintained the crystal shape of the oxide precursor and had a single-crystalline nature. For the BasNb2Ta2015 crystals, the wavelength of the absorption edge was about 707 nm. Especially, the CoOx-Ioaded BaNb_0.5Ta_0.5O_2N photocatalyst demon- strated the comparatively high amount of O_2 gas （150.7 μmol） during the 5 h visible-light-induced sacri- ficial water oxidation half-reaction, which might be achieved due to the high crystallinity and visible-light absorotion property.

关键词： Substitution Flux method Perovskite Oxygen Photocatalyst Solar energy

Strategies and Methods of Modulating Nitrogen-Incorporated Oxide Photocatalysts for Promoted Water Splitting

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Accounts of Materials Research 2022年第4期3卷 449-460页

作者： Yunfeng Bao Can li Kazunari domen Fuxiang Zhang State Key laboratory of Catalysis iChEMDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian National Laboratory for Clean EnergyDalian 116023 LiaoningChina University of Chinese Academy of Sciences Beijing 100049China Research Initiative for Supra-Materials Interdisciplinary Cluster for Cutting Edge ResearchShinshu UniversityNagano-shi 390-0802 NaganoJapan Office of University Professors The University of TokyoTokyo 153-8902Japan

CONSPECTUS:The conversion of solar energy to chemicals and the storage of this energy is one of the most promising routes to realizing a“carbon zero”society,for which particulate photocatalytic water splitting to produce hydrogen has been considered to be a clean potential *** this purpose,many d^(0)and d^(10)metal-based nitrogen-incorporated oxide(including nitrogen-doped oxide and oxynitride)semiconductor photocatalysts have been developed as good candidates to harvest major visible regions of solar light and to exhibit suitable energy levels to drive water *** to the oxide-type photocatalysts,valence bands of the nitrogen-incorporated oxide can be upshifted in consideration of the lower electronegativity of nitrogen atoms concerning the oxygen atoms.

关键词： concerning visible bands

The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

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Journal of Oceanology and limnology 2018年第4期36卷 1091-1102页

作者： ROZINA Tinkara ElERSEK Tina Maja ZUpaNCIC JUSTIN MEGlIC Andrej lESTAN domen SEDMAK Bojan Envit d.o.o. Vojkova cesta 63 1000 Ljubljana Slovenia Department of Genetic Toxicology and Cancer Biology National Institute of Biology Vecna pot 111 1000 Ljubljana Slovenia Arhel d.o.o. Pustovrhova ulica 15 1000 Ljubljana Slovenia Biotechnical Faculty University of Ljubljana Vecna pot 111 1000 Ljubljana Centre for Soil and Environmental Sciences Biotechnical Faculty University of Ljubljana Jamnikarjeva 101 1000 Ljubljana Slovenia

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.

关键词： Microcystis aeruginosa electrochemical oxidation cyanotoxins fluorescence chlorophyll a phycocyanin boron-doped diamond electrode

Regression model for stabilization energies associated with anion ordering in perovskite-type oxynitrides

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Journal of Energy Chemistry 2019年第9期28卷 7-14页

作者： Masanori Kaneko Mikiya Fujii Takashi Hisatomi Koichi Yamashita Kazunari domen Department of Chemical System Engineering School of Engineering The University of Tokyo7-3-1 Hongo Bunkyo-ku Tokyo 113-8656 Japan Center for Energy & Environmental Science Interdisciplinary Cluster for Cutting Edge Research Shinshu University4-17-1 Wakasato Nagano-shi Nagano 380-8553 Japan

Certain perovskite-type oxynitrides have bandgaps suitable for renewable hydrogen production via photocatalytic and photoelectrochemical water splitting under visible *** the ordering of oxide and nitride anions in these materials is important because this ordering affects their semiconductor ***, the numerous possible orderings complicate systematic analyses based on density functional theory(DFT) calculations using defined elemental *** work shows that anion ordering in large-scale supercells within perovskite-type oxynitrides can be rapidly predicted based on machine learning, using BaNbO2N(capa.le of oxidizing water under irradiation up to 740 nm) as an *** learning allows the calculation of the total energy of BaNbO2N directly from randomly selected initial atomic placements without costly structural optimization, thus reducing the computational cost by more than 99.99%.Combined with the Metropolis Monte Carlo method, machine learning permits exploration of the stable anion orderings of large supercells without costly DFT *** work therefore demonstrates a means of predicting the properties of functional materials having complex compositions based on the most realistic elemental arrangements in conjunction with reasonable computational loads.

关键词： Machine learning Total energy Bandgap local anion ordering Density functional theory

Giant primary angiosarcoma of the small intestine showing severe sepsis

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World Journal of Gastroenterology 2014年第43期20卷 16359-16363页

作者： Mizuna Takahashi Masanori Ohara Noriko Kimura Hiromitsu domen Takumi Yamabuki Kazuteru Komuro Takahiro Tsuchikawa Satoshi Hirano Nozomu Iwashiro Department of Surgery National Hospital Organization Hakodate HospitalHakodate 041-8512Japan pathology National Hospital Organization Hakodate HospitalHakodate 041-8512Japan Department of Gastroenterological Surgery II Hokkaido University HospitalHokkaido 060-8648Japan

Primary malignant tumors of the small intestine are rare, comprising less than 2% of all gastrointestinal tumors. An 85-year-old woman was admitted with fever of 40 °C and marked abdominal distension. Her medical history was unremarkable, but blood examination showed elevated inflammatory markers. Abdominal computed tomography showed a giant tumor with central necrosis, extending from the epigastrium to the pelvic cavity. Giant gastrointestinal stromal tumor of the small intestine communicating with the gastrointestinal tract or with superimposed infection was suspected. Because no improvement occurred in response to antibiotics, surgery was performed. laparotomy revealed giant hemorrhagic tumor adherent to the small intestine and occupying the peritoneal cavity. The giant tumor was a solid tumor weighing 3490 g, measuring 24 cm × 17.5 cm × 18 cm and showing marked necrosis. Histologically, the tumor comprised spindle-shaped cells with anaplastic large nuclei. Immunohistochemical studies showed tumor cells positive for vimentin, CD31, and factor VIII-related antigen, but negative for c-kit and CD34. Angiosarcoma was diagnosed. Although no postoperative complications occurred, the patient experienced enlargement of multiple metastatic tumors in the abdominal cavity and died 42 d postoperatively. The prognosis of small intestinal angiosarcoma is very poor, even after volume-reducing pa.liative surgery.

关键词： Angiosarcoma Small intestine Prognosis Sepsis Immunohistological marker

Characterization of acquired nutlin-3 resistant non-small cell lung cancer cells

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Cancer Drug Resistance 2021年第1期4卷 233-243页

作者： Christophe Deben laurie Freire Boullosa Andreas domen An Wouters Bart Cuypers Kris laukens Filip lardon patrick pauwels Center for Oncological Research(CORE) Integrated Personalized&Precision Oncology Network(IPPON)University of AntwerpWilrijk 2610Belgium Adrem Data lab Department of Computer ScienceUniversity of AntwerpAntwerp 2020Belgium Molecular parasitology Unit Department of Biomedical SciencesInstitute of Tropical MedicineAntwerp 2000Belgium Department of pathology Antwerp University HospitalEdegem 2650Belgium

Aim:The purpose of this manuscript is to study the potential characteristics of acquired nutlin-3 resistant non-small cell lung cancer cells(NSClC).Nutlin-3 is an inhibitor of the murine-double minute 2 protein,the main negative regulator of wild type p53,of which several derivatives are currently in clinical ***:A549 NSClC cells were exposed to increasing concentrations of nutlin-3 for a period of 18 *** derivates were cultured,and the most resistance subclone was selected for whole transcriptome *** set enrichment analysis was performed on differentially expressed genes between A549 nutlin-3 resistant cancer cells and the parental A549 p53 wild type cancer *** findings were validated at the gene,protein and/or functional ***:All nutlin-3 resistant subclones acquired mutations in the TP53 gene,resulting in overexpression of the mutant p53 *** most resistant subclone was enriched for genes related to epithelial to mesenchymal transition(EMT),resulting in increased migratory and invasive ***,these cells were enriched in genes related to inflammation,tissue remodelling,and ***,expression of several immune checkpoints,including PD-l1 and PD-l2,was significantly upregulated,and cisplatin-induced cell death was ***:Transcriptome analysis of a highly nutlin-3 resistant A549 subclone shows the relevance of studying(1)resistance to standard of care chemotherapy;(2)secretion of immunomodulating chemo-and cytokines;(3)immune checkpoint expression;and(4)EMT and invasion in nutlin-3 resistant cancer cells in addition to acquired mutations in the TP53 gene.

关键词： p53 murine-double minute 2 non-small cell lung cancer acquired resistance nutlin-3

Efficient visible-light-driven water oxidation by single-crystal Ta_(3)N_(5)nanoparticles

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Nano Research 2023年第4期16卷 4562-4567页

作者： Zheng Wang Jeongsuk Seo Takashi Hisatomi Mamiko Nakabayashi Jiadong Xiao Shanshan Chen lihua lin Zhenhua pan Mary Krause Nick Yin Gordon Smith Naoya Shibata Tsuyoshi Takata Kazunari domen Research Initiative for Supra-Materials Interdisciplinary Cluster for Cutting Edge ResearchShinshu University4-17-1 WakasatoNagano-shiNagano 380-8553Japan Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China Institute of Engineering Innovation The University of Tokyo2-11-16 YayoiBunkyo-kuTokyo 113-8656Japan Global Advanced Metals Inc. 1223 County Line RoadBoyertownPA 19512USA Office of University Professors The University of Tokyo2-11-16 YayoiBunkyo-kuTokyo 113-8656Japan

Ta3N5 is regarded as a promising photocatalyst for solar water splitting because of its excellent visible light absorption characteristics and simple *** Ta3N5 photocatalysts prepared from oxide precursors typically comprise aggregated polycrystalline particles with defects and grain boundaries that reduce the water oxidation activity of the *** the present work,well-dispersed Ta3N5 nanoparticulate single crystals were synthesized via a mild nitridation process using pure Ta metal nanopowder or Ta nanopowder mixed with *** resulting high-quality Ta3N5 nanoparticles,after loading with an oxygen evolution cocatalyst,exhibited impressively high photocatalytic performance during O_(2)evolution from a sacrificial AgNO3 solution,with an apparent quantum yield of 9.4%at 420 *** findings suggest a new approach to the facile fabrication of nanostructured single-crystal photocatalysts for efficient solar water splitting,based on the use of metal nanopowders.

关键词： Ta3N5 photocatalyst nanoparticle single crystal O_(2)evolution metallic Ta precursor