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Advances in Microbiology 2013年第3期3卷 227-232页

作者： Ortiz-Muniz Benigno Corro-Herrera Victor Gomez-Rodriguez Javier domínguez-gonzález josémanuel Aguilar-Uscanga María Guadalupe Instituto Tecnológico Superior de Tierra Blanca Av.Veracruz s/n.Col.PEMEXVeracruzMéxico Instituto Tecnológico de Veracruz Unidad de Investigación y Desarrollo en Alimentos(UNIDA)Dpto Ing.Eléctrica y ElectrónicaCol.Formando HogarVeracruzMéxico Universidad de Vigo Departamento de Ingeniería QuímicaFacultad de CienciasCampus OurenceOurenseSpain

Acetic acid can be directly produced from glucose in one-step fermentation by using yeasts of the genus Brettanomyces bruxellensis, hence increasing the industrial application to manufacture products with simplified bioprocesses. Thereby, this work evaluates the influence of initial glucose concentration on the growth and acetic acid production by B. bruxellensis. The results obtained confirmed the presence of Crabtree effect on B. bruxellensis under low glucose concentrations. The maximum acetic acid concentration reached was 15.4 g·L-1 starting with 100 g·L-1 leading to a product yield of 0.154 g·g-1 and a specific acetic acid production rate of 0.05 g·g-1·h-1. The results also indicate that after reaching the acetic acid critic threshold of 4 g·L-1 the metabolism can induce the growth second phase even residual glucose was present on the culture media at high starting glucose concentrations. Additionally, it was observed a lineal relationship between cell viability and acetic acid production.

关键词： Glucose Brettanomyces bruxellensis Acetic Acid Inhibition Crabtree Effect

Fully integrated and broadband Si-rich silicon nitride wavelength converter based on Bragg scattering intermodal four-wave mixing

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Photonics Research 2024年第3期12卷 I0001-I0010页

作者： VALERIO VITALI THALÍA domÍnguez BUCIO HAO LIU josÉmanuel LUQUE gonzÁlez FRANCISCO JURADO-ROMERO ALEJANDRO ORTEGA-MOÑUX GLENN CHURCHILL JAMES C.GATES JAMES HILLIER NIKOLAOS KALFAGIANNIS DANIELE MELATI JENS H.SCHMID ILARIA CRISTIANI PAVEL CHEBEN J.gonzALO WANGÜEMERT-PÉREZ ÑIGO MOLINA-FERNÁNDEZ FREDERIC GARDES COSIMO LACAVA PERIKLIS PETROPOULOS Optoelectronics Research Centre University of SouthamptonSouthampton SO171BJUK Electrical Computer and Biomedical Engineering DepartmentUniversity of PaviaPavia 27100Italy Telecommunication Research Institute(TELMA) Universidad de MálagaCEI Andalucı´a TECHE.T.S.I.Telecomunicación29010 MálagaSpain School of Science and Technology Nottingham Trent UniversityNottingham NG118NSUK Department of Electrical Engineering Eindhoven University of TechnologyEindhoven 5600 MBThe Netherlands Department of Materials Science and Engineering University of IoanninaIoannina 45110Greece Centre de Nanosciences et de Nanotechnologies UniversitéParis-SaclayCNRS91120 PalaiseauFrance Advanced Electronics and Photonics Research Center National Research Council CanadaOttawaOntario K1A 0R6Canada

Intermodal four-wave mixing(FWM)processes have recently attracted significant interest for all-optical signal processing applications thanks to the possibility to control the propagation properties of waves exciting distinct spatial modes of the same *** allows,in principle,to place signals in different spectral regions and satisfy the phase matching condition over considerably larger bandwidths compared to intramodal ***,the demonstrations reported so far have shown a limited bandwidth and suffered from the lack of on-chip components designed for broadband manipulation of different *** demonstrate here a silicon-rich silicon nitride wavelength converter based on Bragg scattering intermodal FWM,which integrates mode conversion,multiplexing and de-multiplexing functionalities *** system enables wavelength conversion between pump waves and a signal located in different telecommunication bands(separated by 60 nm)with a 3 dB bandwidth exceeding 70 nm,which represents,to our knowledge,the widest bandwidth ever achieved in an intermodal FWM-based system.

关键词： scattering pump modal

Risk Governance,Assessment,and Economic Impacts

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International Journal of Disaster Risk Science 2017年第3期8卷 235-236页

作者： Paula Teves-Costa josémanuel Mendes Instituto dom Luiz Faculdade de Ciências Universidade de Lisboa European Center on Urban Risks (CERU) Center for Social Studies Faculty of Economics University of Coimbra

Due to climate change,growing urbanization,and population migration the social and economic impacts of natural hazards have been increasing *** challenge for risk science,both methodologically and conceptually,is to i... 详细信息

关键词： Risk Governance,Assessment,and Economic Impacts

Assessment of the exploitable biomass of thread herring(Opisthonema spp.)in northwestern Mexico

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Acta Oceanologica Sinica 2021年第9期40卷 53-65页

作者： Marcelino Ruiz-dominguez Casimiro Quinonez-Velazquez Dana Isela Arizmendi-Rodriguez Victor manuel Gomez-Munoz manuel Otilio Nevarez-Martinez Facultad de Ciencias del Mar Universidad Autonoma de SinaloaMazatlan 82000Mexico Instituto Politecnico Nacional Centro Interdisciplinario de Ciencias MarinasLa Paz 23000Mexico Instituto Nacional de Pesca y Acuacultura Centro Regional de Investigacion Pesquera Unidad GuaymasGuaymas 85400Mexico

In recent years,the small pelagic fishery on the Pacific northwest coast of Mexico has significantly increased fishing pressure on thread herring Opisthonema *** fishery is regulated using a precautionary approach(acceptable biological catch(ABC)and minimum catch size).However,due to fishing dynamics,fish aggregation habits and increased fishing mortality,periodic biomass assessments are necessary to estimate ABC and assess the resource *** Catch-MSY approach was used to analyze historical series of thread herring catches off the western Baja California Sur(BCS,1981–2018)and the Gulf of California(GC,1972–2018)to estimate exploitable biomass and target reference points in order to obtain catch *** to the results,in GC,the maximum biomass reached in 1972(at the beginning of fishery)and minimum biomass reached in 2015;the estimated exploitable biomass for 2019 was 42.2×10^(4) t;and the maximum sustainable yield(MSY)was 15.4×10^(4) *** the western BCS coast,the maximum biomass was reached in 1981(at the beginning of fishery)and minimum biomass was reached in 2017;the estimated exploitable biomass for 2019 was 3.2×10^(4) t;and the MSY was 1.2×10^(4) *** stocks showed a decrease in biomass over the past years and were currently near to point of full *** results suggest that the use of the Catch-MSY method is suitable to obtain annual biomass estimates,in order to establish an ABC,to know the current state of the resource,and to avoid overcoming the potential recovery of the stocks.

关键词： Catch-MSY thread herring exploitable biomass estimate

Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-gonzález Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos joséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez josémanuel Lomas Claudio Bueno Rosario Amaya joséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora gonzález-Estrada Demetrio gonzález Carmen Gómez-gonzález Rocío gonzález-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén joséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain Department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain Department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease *** this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 ***,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 Dendritic cell Long-COVID

Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer

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Engineering 2021年第10期7卷 1393-1412页

作者： Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma domínguez Ana Guzman-Aranguez Alberto Peláez-García josémanuel Pingarrón Susana Campuzano Rodrigo Barderas Chronic Disease Program(UFIEC) Carlos III Health InstituteMadrid E-28220Spain Department of Biochemistry and Molecular Biology Faculty of Optics and OptometryComplutense University of MadridMadrid E-28040Spain Gastroenterology Unit San Carlos Clinical HospitalMadrid E-28040Spain Surgical Pathology Department San Carlos Clinical HospitalMadrid E-28040Spain Translational Oncology Division OncoHealth InstituteJimenez Diaz Foundation University HospitalMadrid E-28040Spain Department of Biochemistry Faculty of MedicineAlberto Sols Institute of Biomedical ResearchCSIC-UAMMadrid E-28029Spain Department of Analytical Chemistry Faculty of Chemical SciencesComplutense University of MadridMadrid E-28040Spain Center for Biological Research Spanish National Research Council(CSIC)MadridE-28040Spain Molecular Pathology and Therapeutic Targets Group La Paz University Hospital(IdiPAZ)Madrid E-28046Spain

Colorectal cancer(CRC)is the second leading cause of cancer-related death *** five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced *** against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early ***,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid *** that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to *** bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC *** validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC *** together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here

关键词： Autoantibodies Diagnosis Colorectal cancer Exosomes Tumor microenviroment Humoral immune response Point of care Biosensors

Effectiveness of infliximab after adalimumab failure in Crohn's disease

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World Journal of Gastroenterology 2012年第37期18卷 5219-5224页

作者： María Chaparro Montserrat Andreu manuel Barreiro-de Acosta Esther García-Planella Elena Ricart Eugeni domènech María Esteve Olga Merino Pilar Nos Mireia Pealva Javier P Gisbert Department of Gastroenterology Hospital Universitario de La PrincesaInstituto de Investigación Princesa(IP)and Centro de Investigación Biomédica en Red de Enfermedades Hepáticasy Digestivas(CIBEREHD)28006 MadridSpain Americio 17 portal E 2℃28021 Madrid Department of Gastroenterology Hospital del Mar Department of Gastroenterology Hospital Clínico Universitario de Santiago Department of Gastroenterology Hospital Santa Creui Sant Pau Department of Gastroenterology Hospital ClínicBarcelonaand Centro de Investigación Biomédica en Red de Enfermedades Hepáticasy Digestivas Department of Gastroenterology Hospital Germans Triasi Pujol and Centro de Investigación Biomédica en Red de Enfermedades Hepáticasy Digestivas(CIBEREHD) Department of Gastroenterology Hospital Mutua de Terrassa Department of Gastroenterology Hospital de Cruces Department of Gastroenterology Hospital Universitario La Fe and Centro de Investigación Biomédica en Red de Enfermedades Hepáticasy Digestivas(CIBEREHD) Department of Gastroenterology Hospital de Bellvitge Department of Gastroenterology Hospital Universitario de La PrincesaInstituto de Investigación Princesa(IP)and Centro de Investigación Biomédica en Red de Enfermedades Hepáticasy Digestivas(CIBEREHD)

AIM:To evaluate the effectiveness of infliximab as a second-line therapy in Crohn's disease patients after adalimumab failure. METHODS:A historical cohort study in a community-based gastroenterology practice evaluated Crohn's disease patients treated with infliximab (induction plus maintenance) after adalimumab failure. Patients were identified using a large Spanish database (ENEIDA). RESULTS:We included 15 Crohn's disease patients who received infliximab after adalimumab failure. Five patients discontinued adalimumab due to loss of response, 3 due to adverse events and 7 due to partial response. After infliximab therapy was started, all patients who had interrupted adalimumab due to loss of efficacy regained response. All patients who discontinued adalimumab due to adverse events responded to infliximab and maintained this response; one of these patients had an uneventful course on infliximab, but 2 developed adverse events. None of the 7 patients who interrupted adalimumab due to partial response reached remission with infliximab. CONCLUSION:Switching from adalimumab to infliximab may be useful in patients who develop adverse effects or loss of response, however, the benefit of infliximab in primary nonresponders was not established.

关键词： Adalimumab Biologics Crohn's disease In-fliximab Switch

Management and outcomes of patients with Crohn’s disease with first vs multiple surgeries:results from the PRACTICROHN study

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Gastroenterology Report 2019年第6期7卷 411-418,I0001,I0002页

作者： Marisa Iborra Berta Juliá Maria Dolores Martín Arranz manuel Barreiro-de Acosta Ana Gutiérrez Valle García-Sánchez Carlos Taxonera Javier P.Gisbert Luis Cea-Calvo Eugeni domènech Gastroenterology Department Hospital La FeValenciaSpain Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas(CIBERehd) MadridSpain Medical Department Merk Sharp and Dohme(MSD)MadridSpain Gastroenterology Unit Hospital Universitario La PazIdiPAZMadridSpain Gastroenterology Unit Complejo Hospitalario Universitario de SantiagoSantiago de CompostelaSpain Digestive Medicine Department Hospital General de AlicanteAlicanteSpain Unit of Clinical Management of Gastrointestinal Tract Hospital Universitario Reina SofiaMaimonides Institute of Biomedical Research of Cordoba(IMIBIC)Cordoba UniversityCordobaSpain Gastroenterology Department Hospital Clínico San CarlosMadridSpain Instituto de Investigación del Hospital Clínico San Carlos(IdISSC) MadridSpain Gastroenterology Department Hospital Universitario de La PrincesaInstituto de Investigación Sanitaria Princesa(IIS-IP)MadridSpain Gastroenterology Department Hospital Universitari Germans Trias i PujolBadalonaSpain

Background:Surgery in Crohn’s disease(CD)may be associated with poor prognosis and clinical and surgical *** aim of this study was to describe and compare the post-operative management and outcomes of patients with CD who underwent first vs recurrent ***:Observational study that included adult CD patients from 26 Spanish hospitals who underwent ileocolonic resection with ileocolonic anastomosis between January 2007 and December *** were retrospectively collected from the medical ***:Data from 314 patients were analysed,of whom 262(83%)underwent first surgery and 52(17%)referred to previous CD *** characteristics were similar between the two groups except for a higher rate of stricturing behavior at diagnosis among re-operated patients(P=0.03).After surgery,a higher proportion of re-operated patients received prophylactic treatment with immunomodulators compared with patients with first surgery(P=0.04).In re-operated patients,time to clinical recurrence was not associated with the fact of receiving or not prophylaxis,whereas,in patients with first surgery,recurrence-free survival was greater when prophylaxis was received(P=0.03).Conclusions:After surgery,a higher proportion of patients with previous surgeries received prophylactic treatment with immunomodulators compared with patients with first *** prophylactic treatment was beneficial for preventing clinical recurrence in patients operated on for the first time,it did not significantly reduce the risk of further recurrence in patients with previous *** suggests that effective prophylactic therapies are still needed in this subset of patients.

关键词： Crohn’s disease surgery post-operative recurrence

Tropical tree community composition and diversity variation along a volcanic elevation gradient

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Journal of Mountain Science 2022年第12期19卷 3475-3486页

作者： Ruben MARTINEZ-CAMILO manuel MARTINEZ-MELENDEZ Nayely MARTINEZ-MIELENDEZ Derio Antonio JIMENEZ-LOPEZ Mauricio josE-RIOS Unidad Villa Corzo Facultad de IngenieríaUniversidad de Ciencias y Artes de Chiapas30520 Villa CorzoChiapasMexico Instituto de Ciencias Biológicas Universidad de Ciencias y Artes de Chiapas29039 Tuxtla GutiérrezChiapasMexico Eizia A.C. 29045 Tuxtla GutiérrezChiapasMexico Departamento de Conservación de la Biodiversidad El Colegio de la Frontera Sur29290 San Cristóbal de las CasasChiapasMexico Orquidiario y Jardín Botánico de Comitán Secretaría de Medio Ambiente e Historia Natural30106 Comitán de DomínguezChiapasMexico Instituto de Investigación en Gestión de Riesgos y Cambio Climático Universidad de Ciencias y Artes de Chiapas29039 Tuxtla GutiérrezChiapasMexico

Unraveling the factors that determine variation of diversity in tropical mountain systems is a topic for debate in plant *** is especially true in areas where topography is complex due to volcano elevational gradients and where forests are vulnerable to human *** this study we used a set of climatic(temperature,rainfall,and radiation solar),topographic(elevation,slope aspect,and slope orientation)and human disturbance variables to determine their effect on diversity and composition patterns of a tree community,considering three slope aspects of a tropical volcano in southeastern *** sampled trees in seventy 0.1-ha plots distributed on three slope aspects of the Tacanávolcano along an elevational gradient of 1500 to 2500 *** determined diversity patterns(general tree richness,exponential of Shannon index,and pioneer species richness)with linear regression models,and for beta diversity,we used a dissimilarity index(within and between elevational bands 100 m wide).The effect of a set of environmental and human disturbance variables on tree diversity and community composition was analyzed with general linear models and multivariate analyses,*** registered 2,949 individual trees belonging to 176 species and *** average species richness and alpha diversity per plot were 13(standard deviation±6)and 9(±5),*** tree richness and alpha diversity increased in the middle part(unimodal patterns)of the elevational gradient,but pioneer species richness decreased linearly with *** variance explained by general linear models was greater in richness(32%)than in alpha diversity(25.3%).The most important predictor variables were temperature(elevational gradient),which explained the unimodal pattern(richness and alpha diversity increase at intermediate levels of temperature),and slope orientation,which explained the increase in richness and alpha diversity toward the geographic *** temperature had a signific

关键词： Composition Environmental gradient Tree diversity patterns Tropical humid cloud forest Tropical volcanoes

Molecular Identification of Hammerhead Shark Trunks from the Southern Gulf of California using Multiplex PCR

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Journal of Fisheries Science 2020年第1期2卷 22-29页

作者： Thelma A.Aguilar-Rendón J.Juan Rendón-Herrera Virginia Osuna-gonzález Erick C.Oñate-Gónzalez Omar domínguez-domínguez Nancy C.Saavedra-Sotelo Laboratorio de Ecología Molecular Facultad de Ciencias del MarUniversidad Autónoma de Sinaloa.Paseo Claussen s/nMazatlánSinaloa.C.P.82000.México Laboratorio de Ictiología Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónAv.Pedro de Alba y Manuel L.Barragans/nCd UniversitariaSan Nicolás de los GarzaNuevo León.C.P.66455.México Laboratorio de Biología Acuática Universidad Michoacana de San Nicolás de Hidalgo.Edificio RPlanta BajaCiudad UniversitariaMoreliaMichoacán.C.P.58030.México Cátedras-CONACyT Consejo Nacional de Ciencia y TecnologíaAv.Insurgentes Sur No.1582Del.Benito JuárezCDMX.C.P.03940.México

The demand for shark fins in Asiatic markets has resulted in excessive increases in shark catches,even for species that may be under protection or subject to *** such,it has been necessary to develop and promote monitoring efforts for exploited species and taxonomic groups in order to improve fishing management strategies for *** species from landings is one of many fishing management problems because landed organisms have usually already been processed and are therefore incomplete,which makes identification problematic,impedes the generation of proper species records,and leads to poor fishery *** that can correctly identify species,such as various molecular techniques,have become essential for accurate fishery *** this study,30 hammerhead trunks from artisanal fisheries from the southern portion of the Gulf of California were identified using multiplex PCR(17 Sphyrna lewini and 13 Sphyrna zygaena).The total fee to identify each trunk with this technique was~$3.80 and the procedure required 2 to 5 *** compared with other widely-used methods,such as PCR-RFLP or barcoding,multiplex PCR is fast,efficient,low-cost,and easy to implement in a laboratory.

关键词： Sphyrna ITS2 Molecular marker Ribosomal DNA Electrophoreses