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multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer

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Engineering 2021年第10期7卷 1393-1412页

作者： Ana montero-Calle Itziar Aranguren-Abeigon maría Garranzo-Asensio Carmen Poves maría Jesús Fernández-Aceñero Javier martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano maria Gamella Rebeca magnolia Torrente-Rodríguez miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma domínguez Ana Guzman-Aranguez Alberto Peláez-García Josémanuel Pingarrón Susana Campuzano Rodrigo Barderas Chronic Disease Program(UFIEC) Carlos III Health InstituteMadrid E-28220Spain Department of Biochemistry and molecular Biology Faculty of Optics and OptometryComplutense University of MadridMadrid E-28040Spain Gastroenterology Unit San Carlos Clinical HospitalMadrid E-28040Spain Surgical Pathology Department San Carlos Clinical HospitalMadrid E-28040Spain Translational Oncology Division OncoHealth InstituteJimenez Diaz Foundation University HospitalMadrid E-28040Spain Department of Biochemistry Faculty of MedicineAlberto Sols Institute of Biomedical ResearchCSIC-UAMMadrid E-28029Spain Department of Analytical Chemistry Faculty of Chemical SciencesComplutense University of MadridMadrid E-28040Spain Center for Biological Research Spanish National Research Council(CSIC)MadridE-28040Spain molecular Pathology and Therapeutic Targets Group La Paz University Hospital(IdiPAZ)Madrid E-28046Spain

Colorectal cancer(CRC)is the second leading cause of cancer-related death *** five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced *** against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early ***,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid *** that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to *** bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC *** validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC *** together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here

关键词： Autoantibodies Diagnosis Colorectal cancer Exosomes Tumor microenviroment Humoral immune response Point of care Biosensors

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1:5 0000 SCALE TOPOGRAPHIC mAPPING IN WEST CHINA USING SPOT 5 DATA

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测绘科学 2008年第S2期33卷 25-27,9页

作者： m.Bernard

On the one hand,the successful launch of the SPOT 5 satellite in 2002 can be seen as a milestone for DEm extraction and mapping,because its payload includes HRS,a stereoscopic imaging instrument devoted to collection of large *** in panchromaticmode,the HRS instrument has two telescopes,one pointing forward and one aft of the *** is thus able to cover an area of 600 km×120 km in a single pass(ie 72000 km^2 stereoscopic strips).moreover,SPOT 5 is able to collect stereoscopic triplets,through a simultaneousactivation of HRG sensors in vertical mode,which will overcome the difficulty of terrain shadow in DEm *** the extremelyprecise ancillary metadata of SPOT 5 allows accurate geometric processing with sparse ground control *** SPOT 5 stereoscopicimagery becomes one of main satellite data sources for accurate DEm extraction and quickly mapping large *** current status and coverageof stereoscopic HRS imagery as well as the metadata of SPOT 5 will be briefly *** the second hand,about 2 million km^2 in west China never has been covered by 1:50000 scale topographic maps for several *** area contains the famous Qingzang plateau with average 5000m altitude and boundless Talimu basin *** this area withtraditional methods had unimaginable difficulties and was impossible to complete at *** the high technology advancing,especially thegreat progresses in geomatics,it is the time to start to map the area with new geo-information *** in 2006,China decided to initiatea large project,named 1:50000 scale topographic mapping of west China(*** China topographic mapping project),in order to getthe 1:50000 scale topographic maps(DEm,dom and DLG)of that area within next 5 *** we know the weather is extremely atrociousand a lot of sites are out of reach in that area,mapping from aero-born and satellite remote sensing imagery with spares ground control pointswas determined to be the main so

关键词： mapping SPOT geometric modelling DEm accuracy

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WJD 5^(th) Anniversary Special Issues(2): Type 2 diabetes Inflammation in diabetic kidney disease

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World Journal of Diabetes 2014年第4期5卷 431-443页

作者： Patricia m García-García maría A Getino-melian Virginia domínguez-Pimentel Juan F Navarro-Gonzalez Nephrology Service and Research Unit University Hospital Nuestra Se?ora de Candelaria 38010 Santa Cruz de Tenerife Spain Spanish National Coordinator of GEENDIAB (Grupo Espa ol para el Estudio de la Nefropatía Di-abética) (RedInRen 12/0021/0019 Instituto de Salud Carlos IIIMinisterio de Economía y Competitividad) 38010 Santa Cruz de Tenerife Spain

Diabetes mellitus entails significant health problems *** pathogenesis of diabetes is multifactorial,resulting from interactions of both genetic and environmental factors that trigger a complex network of pathophysiological events,with metabolic and hemodynamic *** this context,inflammation has emerged as a key pathophysiology *** pathogenic pathways will provide targets for prevention or future *** review will focus on the implications of inflammation in diabetes mellitus,with special attention to inflammatory cytokines.

关键词： Diabetes Diabetic nephropathy Diabetic kidney disease Inflammation Cytokines Oxidative stress

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2011年耶罗火山喷发前的地震活动模式

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世界地震译丛 2015年第1期46卷 35-45页

作者： I.domínguez Cerdea C.del Fresno A.Gomis moreno 欧阳兆国吴宝峰杜微黑龙江省地震局辽宁省海城地震台

在加那利群岛第一个被完全监测的火山于2011年10月10日在耶罗岛以南2km处开始喷发。这次海底火山喷发之前有各种各样的前兆信号,其中最明显的是在2011年7月开始出现的由人工拾取定位的近10 000个低震级地震组成的地震序列。这个地震活... 详细信息

在加那利群岛第一个被完全监测的火山于2011年10月10日在耶罗岛以南2km处开始喷发。这次海底火山喷发之前有各种各样的前兆信号,其中最明显的是在2011年7月开始出现的由人工拾取定位的近10 000个低震级地震组成的地震序列。这个地震活动序列揭示出从耶罗岛以北向南在10~17km之间的深度有20km的水平迁移,越向南地震的深度越深。我们应用相对定位算法（hypoDD）提高震源定位的精度。由此产生的地震分布更加紧凑,震源分布稍浅,并揭示了从北向南震源深度的明显差别。为了检查这些结果的可靠性所进行的检验给出重定位的最大不确定性为400m。此外还发现了新特征,包括在该岛中心地震活动的起源和在该岛以北在活动的最初一个月期间存在两个交替的孕震区。

关键词：在线材料互相关波形图数据统计射线参数立体图射线追踪测试 hypoDD重定位表

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Detection of tyrosine,trace metals and nutrients in cow dung:the environmental significance in soil and water environments

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Acta Geochimica 2018年第4期37卷 632-638页

作者： Khan m.G.mostofa Longlong Li Congqiang Liu Institute of Surface-Earth System Science Tianjin University State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of Sciences

This study examined the dissolved organic matter(dom) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEm)spectroscopy and parallel factor(PARAFAC) modelling along with eleven trace metals using ICP-mS and nutrients(NH_4^+ and NO_3^-) using an AA3 auto analyser. EEm–PARAFAC analysis demonstrated that cow dung predominantly contained only one fluorescent dom component with two fluorescence peaks(Ex/Em=275/311 nm and Ex/Em=220/311 nm),which could be denoted as tyrosine by comparison with its standard. Occurrence of tyrosine can be further confirmed by the FTIR spectra. Trace metals analysis revealed that Na,K and mg were significantly higher than Ca,Fe,mn,Zn Sr,Cu,Ni and Co. The NH_4^+ concentrations were substantially higher than NO_3^-.These results thus indicate that the dissolved components of the cow dung could be useful for better understanding its future uses in various important purposes.

关键词： Cow dung Excitation-emission matrix (EEm)spectroscopy Parallel factor (PARAFAC) modelling Tyrosine Trace metals

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Acceptability Properties and Antioxidant Pontential of Desi Chickpea (<i>Cicer arietinum</i>L.) Cultivars

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Food and Nutrition Sciences 2012年第9期3卷 1281-1289页

作者： José Antonio Garzón-Tiznado mirna Isabel Ochoa-Lugo mar de Jesús Heiras-Palazuelos Dulce maría domínguez-Arispuro Edith Oliva Cuevas Rodríguez Roberto Gutiérrez-Dorado Jorge milán-Carrillo Cuauhtémoc Reyes-moreno maestria en Ciencia y Tecnología de Alimentos Facultad de Ciencias Químico-Biológicas Universidad Autónoma de Sinaloa Sinaloa México master in Food Science and Technology Faculty of Chemical-Biological Sciences Autonomous University of Sinaloa Sinaloa Mexico Programa Regional del Noroeste para el Doctorado en Biotecnología Universidad Autónoma de Sinaloa Sinaloa México

The acceptability properties, total phenolic content, and antioxidant potential of four (red, green, brown, cream) desi chickpea (Cicer arietinum L.) cultivars from the World Germplasm Bank were evaluated. Protein content of the grains varied from 20.12% to 28.85% (dw), highest for Cream ICC3421. The water absorption capacity (WAC) and cooking time (CT) of the whole grains ranged from 97.7 to 117.5 g water/100 g seeds (ww) and from 109.5 to 193.5 min, respectively;the highest WAC and lowest CT corresponded to Cream ICC3421 cultivar. The total phenolic content (TPC) and total hydrophilic antioxidant activity (AoxA) [oxygen radical absorbance capacity (ORAC) value] of desi chickpea cultivars varied from 746 to 1.286 μg gallic acid equivalents (GAE)/g sample (dw) and from 43.9 to 53.9 μmol Trolox equivalents (TE)/g sample (dw);Brown ICC3512 showed the highest ORAC value. Chickpea cultivars could contribute significantly to the management and/or prevention of degenerative diseases associated with free radical damage.

关键词： Desi Chickpeas Cultivars Acceptability Properties Antioxidant Activity Total Phenolic Content

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Enhancement of Corrosion Resistance of NiCrFeBSi Coatings Obtained by Flame Thermal Spray Process Adding an Electroless Nickel Coating Ni-P

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Journal of Surface Engineered materials and Advanced Technology 2017年第4期7卷 86-97页

作者： Arturo Barba-Pingarrón Raúl Valdez-Navarro Félix Sánchez-De Jesus Ana maría Bolarín-miró Rafael González-Parra Alba Covelo-Villar miguel ángel Hernández-Gallegos Carlos domínguez-Ríos Centro de Ingeniería de Superficies y Acabados (CENISA) Facultad de Ingeniería Universidad Nacional Autónoma de México 2 Nivel Edificio “T” (Bernardo Quintana) Circuito Exterior Ciudad Universitaria Ciudad de México México área Académica de Ciencias de la Tierra y materiales Universidad Autónoma del Estado de Hidalgo Pachuca México Centro de Investigación en materiales Avanzados Chihuahua México

Flame Thermal Spray (FTS) coatings frequently show some porosity and reduced adherence to substrate, which affect its properties, especially its corrosion resistance. In this work, the corrosion resistance of AISI 1018 carbon steel coated by different methods is compared: electroless nickel (EN) coating, NiCrFeBSi obtained by FTS, duplex coatings of an EN deposit on a layer of NiCrFeBSi obtained by FTS and a layer of NiCrFeBSi on an EN deposit. The coatings were characterized using optical microscopy and scanning electron microscopy techniques, EDS microprobe microanalysis, roughness as well as electrochemical polarization tests to obtain the corrosion rate. The results show the enhancement in the corrosion resistance in saline medium of the duplex coatings, especially of the EN coating on FTS layer.

关键词： Flame Thermal Spray Coatings Electroless Nickel Plating Duplex Coatings Corrosion Resistance

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三药固定剂量复方制剂的利福平相对生物利用度

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国际结核病与肺部疾病杂志 2011年第2期6卷 85-90页

作者： R.C.milán-Segovia A.m.domínguez-Ramírez H.Jung-Cook m.magaa-Aquino m.C.Romero-méndez S.E.medellín-Garibay m.Vigna-Pérez S.Romano-moreno 金海霞 Universidad Autónoma metropolitana Unidad XochimilcoMexico DF Facultad de Ciencias Químicas Universidad Autónoma de San Luís PotosíSan Luis Potosí Facultad de Química Universidad Nacional Autónoma de MéxicoMexico City Hospital Central 'Dr Ignacio morones Prieto' Secretaría de Salud San Luis PotosíMexico Facultad de Ciencias Químicas Universidad Autónoma de San Luís PotosíSan Luis Potosí

背景:先前的一个利福平(RmP)监测性研究显示,结核病(TB)患者在使用常规的3药固定剂量复方制剂(3FDC)治疗时,RmP浓度很低。因此,我们对该制剂的生物利用度进行研究。目的:利用健康志愿者研究墨西哥卫生保健系统中常规使用的3FDC制剂相对... 详细信息

背景:先前的一个利福平(RmP)监测性研究显示,结核病(TB)患者在使用常规的3药固定剂量复方制剂(3FDC)治疗时,RmP浓度很低。因此,我们对该制剂的生物利用度进行研究。目的:利用健康志愿者研究墨西哥卫生保健系统中常规使用的3FDC制剂相对于参比制剂的RmP相对生物利用度。设计:两周期、两序列的交叉研究。结果:受试制剂和参比制剂的药代动力学参数均值如下:达峰浓度(Cmax)分别为3.13±2.01μg/ml和9.95±2.66μg/ml;对于血药浓度-时间曲线下面积(AUC),零到最后实测浓度的AUC(AUC0-12 h)分别为15.51±9.77μg.h/ml和58.03±16.1μg.h/ml,零到无限大时间的AUC(AUC0-∞)分别为17.92±10.66μg.h/ml和68.43±22.39μg.h/ml。Cmax的受试/参比均值比(90%CI)为25.36%(17.33~37.10);AUC0-12 h为21.25%(14.61~30.89);AUC0-∞为22.08%(15.44~31.56)。这些结果不符合生物等效性标准。结论:受试制剂与参比制剂相比,显示吸收延迟和RmP生物利用度明显偏低...

关键词：相对生物利用度利福平固定剂量复方

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The mechanism behind the dom effects on methylmercury photodegradation

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Science Bulletin 2015年第13期60卷 1220-1221页

作者： Khan m.G.mostofa Cong-Qiang Liu marco minella Davide Vione Institute of Surface Earth System Science Tianjin University State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Dipartimento di Chimica Universita` degli Studi di Torino Centro Interdipartimentale Nat Risk

methyl mercury(CH3Hg+),a neurotoxin,is the most toxic form of mercury that occurs in natural waters[1–4].It is a cause of concern because of increasing worldwide pollution by mercury in both water and atmosphere[3,5]... 详细信息

关键词： dom 甲基汞光降解机制自然水域溶解有机物神经毒素去除途径

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Effect of photo-biodegradation and biodegradation on the biogeochemical cycling of dissolved organic matter across diverse surface water bodies

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Journal of Environmental Sciences 2019年第3期31卷 130-147页

作者： m.S.Sankar Padmanava Dash Shatrughan Singh YueHan Lu ANDrew E.mercer Shuo Chen Department of Geosciences Mississippi State University Department of Geological Sciences University of Alabama Academy for Advanced Interdisciplinary Studies Southern University of Science and Technology

The objective of this research was to quantify the temporal variation of dissolved organic matter(dom) in five distinct waterbodies in watersheds with diverse types of land use and land cover in the presence and absence of sunlight. The water bodies were an agricultural pond, a lake in a forested watershed, a man-made reservoir, an estuary, and a bay. Two sets of samples were prepared by dispensing unfiltered samples into filtered samples in 1:10 ratio(V/V). The first set was exposed to sunlight(10 hr per day for 30 days) for examining the combined effect of photo-biodegradation, while the second set was stored in dark for examining biodegradation alone. Spectroscopic measurements in tandem with multivariate statistics were used to interpret dom lability and composition. The results suggest that the agricultural pond behaved differently compared to other study locations during degradation experiments due to the presence of higher amount of microbial humic-like and protein-like components derived from microbial/anthropogenic sources. For all samples, a larger decrease in dissolved organic carbon(DOC) concentration(10.12% ±9.81% for photo-biodegradation and 6.65% ± 2.83% for biodegradation) and rapid transformation of dom components(i.e., terrestrial humic-like components into microbial humic and protein-like components) were observed during photo-biodegradation *** suggest that sunlight facilitated dom biodegradation, resulting in simpler recalcitrant molecules regardless of original composition. Overall, it was found that combined effects of light and bacteria are more efficient than bacterial effects alone in remineralizing and altering dom, which highlights the crucial importance of sunlight in transforming aquatic dom.

关键词： Photo-biodegradation Biodegradation Dissolved organic matter Land use and land cover Half-life EEm-PARAFAC

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