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A SPECIAL MODULUS OF CONTINUITY AND THE K-FUNCTIONAL

Acta Mathematica Scientia 2016年第5期36卷 1531-1540页

作者： Nadezhda dolmatova Institute of Mathematics University of Wroc law

We consider the questions connected with the approximation of a real continuous 1-periodic functions and give a new proof of the equivalence of the special Boman-Shapiro modulus of continuity with Peetre’s K-function... 详细信息

关键词： modulus of continuity K-functional Jackson's theorem

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Lead Induces Different Responses of Two Subpopulations of Phagocytes in the Holothurian Eupentacta fraudatrix

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Journal of Ocean University of China 2018年第6期17卷 1391-1403页

作者： dolmatova Lyudmila S. DOLMATOV Igor Yu. Laboratory of Biochemistry V.I.Il'ichev Pacific Oceanological Institute Far Eastern Branch Russian Academy of Sciences Laboratory of Comparative Cytology National Scientific Center of Marine Biology Far Eastern Branch Russian Academy of Sciences Department of Cell Biology and Genetics Far Eastern Federal University

In view of increasing lead pollution(Pb^(2+)) of coastal waters, the compensatory abilities of holothurians need to be assessed. The goal of the work is to clarify the functional and phenotypical differences between two types of phagocytes(P1 and P2) in Eupentacta fraudatrix exposed to Pb(NO_3)_2. It has been shown that 2 mg L^(-1) lead exposure for 48 h increases the number of P2 phagocytes as compared to P1 cells, does not significantly affect cell viability in both P1 and P2 phagocyte fractions, and significantly enhances chromatin condensation in P2 but not in P1 phagocytes. A lead concentration of 4 mg L^(-1) increases the number of P1 phagocytes compared to that of P2 type, and does not change cell viability and chromatin condensation in P1 phagocytes. In the P2 type, it decreases cell viability and does not influence the level of apoptosis. The protection against lead-induced apoptosis is apparently mediated by the activities of antioxidant enzymes, especially glutathione S-transferase. The differences in labeling cell surface receptors of P1 and P2 phagocytes by plant lectins also indicate the specific phenotypic properties of these cells. The results clarify the potential and GSH-dependent mechanisms of immune adaptation in holothurians that have been shortly exposed to lead at concentrations close to the maximum environmentally relevant level in coastal waters. Additionally, P1 and P2 phagocytes are first shown to have different functions and phenotypes during the response to lead, which indicates the complexity of the phagocytic system in holothurians and contributes to understanding the immunity evolution.

关键词： amoebocytes antioxidant capacity apoptosis cell adaptation lead plant lectin con A DBA PNA SBA

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Cooperative apoptosis of coelomocytes of the holothurian <i>Eupentacta fraudatrix</i>and its modulation by dexamethasone

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Advances in Bioscience and Biotechnology 2013年第9期4卷 908-917页

作者： Olga A. Zaika Lyudmila S. dolmatova Il’ichev Pacific Oceanological Institute Far Eastern Branch Russian Academy of Sciences Vladivostok Russia

The capacities of phagocytes of subpopulation P1 (F) and morula cells (MC) of holothurian Eupentacta fraudatrix to modulate apoptosis of each other as well as cytokine-dependent mechanisms and hormonal regulation of these cells’s interaction were studied. The 18-h treatment of F with supernatants, obtained after centrifugation of MC preincubated for 3 h with phosphate buffered saline (PSB) at the temperature of 22℃?(SMC3) resulted in a significant growth of apoptosis level. A 30-min incubation of F with supernatants of MC, preincubated for 24 h (SMC24), on the contrary, reduced the apoptosis level and increased the level of interleukine-1α (IL-1α)-like substances, and 24-h incubation did not influence apoptosis and reduced level of IL-1α-like substances. Thus, proapoptotic effects of MC’s supernatants in F inversely depended on time of their preincubation with PSB and directly on time of incubation with F. Additionally, this effect was opposite to variations in the level of IL-1α-like substances. The level of apoptosis declined after 30 min of incubation but elevated after 24 h at the inverse treatment of MC with supernatant, obtained after preincubation of F during 24 h (SF24). The level of IL-1α-like substances dropped after 30 min and insignificantly decreased after 24 h. Hence, SF24 proapoptotic effect directly depended on time of incubation with MC and did not correspond to variations in the level of IL-1α-like substances. 100 μM dexamethasone stimulated apoptosis in F and MC in an inverse time-dependent manner during 24-h preincubation, and supernatants of cell suspensions obtained after such preincubations, stimulated apoptosis and reduced the IL-1α-like substances level in target cells at both types of interaction. IL-1α-like substances are?supposed to be mediators for MC’s effects in F, but not for F’s action on MC. In holothurians, steroid hormones apparently may participate in the regulation of the immune response and cell cooperation.

关键词： Phagocytes Morula Cells Cell Cooperation Dexamethasone Apoptosis Cytokines

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Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro

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Chinese Journal of Oceanology and Limnology 2003年第4期21卷 293-304页

作者： dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A ichev Pacific Oceanological Institute Institute of Marine BiologyResearch Institute of Epidemiology and Microbiologyichev Pacific Oceanological Instituteichev Pacific Oceanological Institute Vladivostok 690041 Russia Vladivostok 690041 Russia  Vladivostok 690087 Russia Vladivostok 690041 Russia Vladivostok 690041 Russia

Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians.

关键词： bacterial toxin coelomocytes holothurian reactive oxygen species superoxide dismutase

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