Pathogen effectors target diverse subcellular organelles to manipulate the plant immune *** the nucleolus has emerged as a stress marker and several effectors are localized in the nucleolus,the roles of nucleolar-targ...
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Pathogen effectors target diverse subcellular organelles to manipulate the plant immune *** the nucleolus has emerged as a stress marker and several effectors are localized in the nucleolus,the roles of nucleolar-targeted effectors remain *** this study,we showed that Phytophthora infestans infection of Nicotiana benthamiana results in nucleolar inflation during the transition from the biotrophic to the necrotrophic *** *** effectors were localized in the nucleolus:Pi23226 induced cell death in *** and nucleolar inflation similar to that observed in the necrotrophic stage of infection,whereas its homolog Pi23015 and a deletion mutant(Pi23226DC)did not induce cell death or affect nucleolar *** immunoprecipitation and individual-nucleotide-resolution UV crosslinking and immunoprecipitation sequencing analysis indicated that Pi23226 bound to the 30 end of 25S rRNA precursors,resulting in accumulation of unprocessed 27S *** nucleolar stress marker NAC082 was strongly upregulated under Pi23226-expressing ***23226 subsequently inhibited global protein translation in host cells by interacting with ***23226 enhanced *** pathogenicity,indicating that Pi23226-induced ribosome malfunction and cell death were beneficial for pathogenesis in the *** results provide evidence for the molecular mechanism underlying RNA-binding effector activity in host ribosome biogenesis and lead to new insights into the nucleolar action of effectors in pathogenesis.
Dofs(DNA-binding with one zinc finger proteins)是植物特有的转录因子家族。Dof蛋白参与许多生物过程,如植物激素的产生,种子发育和环境适应。Dof蛋白已经在多种植物中被鉴定,但在辣椒中尚未开展类似的研究。我们在辣椒中鉴定出了33...
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Dofs(DNA-binding with one zinc finger proteins)是植物特有的转录因子家族。Dof蛋白参与许多生物过程,如植物激素的产生,种子发育和环境适应。Dof蛋白已经在多种植物中被鉴定,但在辣椒中尚未开展类似的研究。我们在辣椒中鉴定出了33个可能的Dof基因(CaDofs)。为了了解CaDof基因的特征,我们分析了其系统进化关系、蛋白质基序和进化历史。我们将含有25个基序且分布在8条染色体上的33个CaDof基因分成4个族。我们发现CaDof基因的扩张可追溯到最近的复制事件。茄科种系形成之前,片段复制在CaDof家族基因中占主导地位。通过RNA-seq分析获得了CaDof基因表达谱,发现在对包括两种TMV菌株,辣椒斑驳病毒和辣椒疫霉菌的生物胁迫响应过程中,Ca Dof家族基因表现出了时间和病原特异性变化,这表明CaDof基因的功能具有多样性。这些结果不仅为研究Dof基因在生物胁迫中的响应提供有价值的信息,同时也有助于更好地理解Dof基因在辣椒和其他物种中的多重功能。
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