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Validation of a serum microRNA panel as biomarkers for early diagnosis of hepatocellular carcinoma post-hepatitis C infection in Egyptian patients

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World Journal of Gastroenterology 2017年第21期23卷 3864-3875页

作者： Moustafa Nouh Elemeery ahmed Noah Badr Marwa Anwar Mohamed doaa ahmed ghareeb Medical Biotechnology Laboratory Genetic Engineering and Biotechnology Research DivisionNational Research Centre Toxicology and Food Contaminant Laboratory Food Science and Nutrition Research DivisionNational Research Centre Department of Chemical Pathology Medical Research InstituteAlexandria University Molecular Biology Laboratory Faculty of ScienceAlexandria University

AIM To investigate the prospective importance of serum micro(mi)RNAs(mi R-125 b, mi R-138 b, mi R-1269, mi R-214-5p, mi R-494, mi R375 and mi R-145) as early biomarkers for the diagnosis of hepatitis C virus(HCV)-related hepatocellular carcinoma(HCC).METHODS Two-hundred and fifty HCV4 a patients, 224 HCV4 aHCC patients, and 84 healthy controls were enrolled in the study. Expression levels of mi R214-5p, mi R-125 b, mi R-1269 and mi R-375 were quantified using quantitative real-time *** Expression of the selected mi RNAs in serum wassignificantly lower in HCC patients than in the healthy controls, except for mi R-1269 and mi R-494. There was a significant difference between HCC and HCV patients, in particular for HCC and late stage fibrosis, rather than HCV patients and early fibrosis. It is obvious that mi R-1269 was significantly upregulated in HCC cases compared to hepatic fibrosis cases. Each mi RNA can show HCC progression. Multivariate logistic regression analysis indicated that the tested panel of mi RNAs(mi R214-5p, mi R-125 b, mi R-1269 and mi R-375) represent accurate and specific indictors of HCC *** This study presents a panel of mi RNAs with strong power as putative diagnostic and prognostic biomarkers for HCV-induced HCC. Moreover, mi R-214-5p and mi R-1269 could be considered as early biomarkers for tracking the progress of liver fibrosis to HCC.

关键词： Micro RNA Hepatocellular carcinoma Fibrosis progression

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MicroRNA signature in patients with hepatocellular carcinoma associated with type 2 diabetes

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World Journal of Gastroenterology 2019年第42期25卷 6322-6341页

作者： Moustafa Nouh Elemeery Marwa Anwar Mohamed Marwa ahmed Madkour Mohammed Mohammed Shamseya Noha Mahmoud Issa ahmed Noah Badr doaa ahmed ghareeb Cheol-Ho Pan Département de Neurosciences CRCHUMUniversitéde MontréalMontréalQuebec H2X 3E4Canada Medical Biotechnology Laboratory Genetic Engineering and Biotechnology Research DivisionNational Research CentreCairo 12622Egypt Natural Product Informatics Research Center KIST Gangneung Institute of Natural ProductsGangneung 25451South Korea Division of Bio-Medical Science and Technology KIST SchoolKorea University of Science and TechnologySeoul 02792South Korea Department of Chemical Pathology Medical Research InstituteAlexandria UniversityAlexandria 21511Egypt Experimental and Clinical Internal Medicine Department Medical Research InstituteAlexandria UniversityAlexandria 21511Egypt Human Genetics Department Medical Research InstituteAlexandria UniversityAlexandria 21511Egypt Food Toxicology and Contaminates Department National Research CentreDokkiCairo 12622Egypt Bioscreening and preclinical trial lab Biochemistry DepartmentFaculty of ScienceAlexandria UniversityAlexandria 12522Egypt Pharmaceutical and fermentation industries development center the city of scientific research and technological applicationsAlexandria 26411Egypt

BACKGROUND Nonalcoholic steatohepatitis-related cirrhosis is one of the liver complications in type 2 diabetes mellitus(T2DM)and reported to be a risk factor for developing hepatocellular carcinoma(HCC).A reliable screening biomarker of liver cirrhosis(LC)and HCC among T2DM patients is important to reduce the morbidity and mortality of this ***(miRNA)is considered a key player in HCC and T2DM,and it might be a hidden culprit in diabetes-associated HCC,making it a promising reliable prognostic *** To investigate the signature of serum miRNAs as early biomarkers for the screening of HCC among diabetic *** Expression profiles of miRNAs in serum samples of diabetic LC and diabetic HCC patients were assessed using Illumina sequencing;then,RT-qPCR was used to validate significantly altered miRNAs between the two *** miRNAs were tested in serum samples of 200 T2DM patients,270 LC patients,200 HCC patients,and 225 healthy control ***,receiver operating characteristic(ROC)analysis,with area under the curve(AUC),was performed to assess the diagnostic performance of the screened miRNAs for discriminating HCC from LC and nonmalignant patients(LC+T2DM).RESULTS Expression of the sequenced miRNAs in serum was different in HCC vs LCpositive T2DM *** miRNAs(miR-34a,miR-221)were significantly upregulated and five miRNAs(miR-16,miR-23-3p,miR-122-5p,miR-198,miR-199a-3p)were significantly down-regulated in HCC compared to LC *** of ROC curve demonstrated that the combination of these seven miRNAs can be used as a reliable biomarker for detection of HCC in diabetic patients,as it could identify HCC with high diagnostic accuracy in diabetic LC patients(AUC=0.993)and in diabetic nonmalignant patients(AUC=0.961).CONCLUSION This study validates a panel of serum miRNAs that can be used as a reliable noninvasive screening biomarker of HCC among T2DM cirrhotic and noncirrhotic *** study recommends furt

关键词： MicroRNA Hepatocellular carcinoma Type 2 diabetes Nonalcoholic fatty liver disease

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Walking experience:Exploring the trilateral interrelation of walkability,temporal perception,and urban ambiance

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Frontiers of Architectural Research 2021年第3期10卷 516-539页

作者： doaa K.Hassan ahmed Elkhateeb Department of Architecture Faculty of EngineeringAin Shams UniversityCairoEgypt

Walking experience through urban spaces is a frequent daily ***,planning and designing for walkability become crucial for building up quality of *** daily walks around the city are affected by fulfilling the needed psychological contentment that is in turn measured unconsciously by our temporal *** contentment is inherent in what we experience in accordance with urban ambiance that goes beyond the spatial attributes of spaces to include their social and sensorial ***,the question here is how walkability of urban routes is affected by urban ambiance and therefore perceived in terms of ***,this study aims to understate the trilateral interrelation of walkability,temporal perception,and urban ambiance in walkable routes for daily routine *** work depends on an experimental study where participants are asked to walk along two selected ***-gathering tools rely on documenting the lived experience of the target group via reporting their trips and answering a *** analysis demonstrates this trilateral interrelation to clarify the *** study concludes specific qualities of walkable routes for routine use that reduce sense of time,which is highly crucial to be realized in such daily walk journeys.

关键词： Walkability Urban ambiance Temporal perception People’s needs in open spaces

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Preparation of PVC/PVP composite polymer membranes via phase inversion process for water treatment purposes

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Chinese Journal of Chemical Engineering 2018年第4期26卷 715-722页

作者： ahmed Bhran Abeer Shoaib doaa Elsadeq Ayman El-gendi Heba Abdallah Chemical Engineering Department College of Engineering Al Imam Mohammad Ibn Saud Islamic University (IMSIU) Department of Petroleum Refining and Petrochemical Engineering Faculty of Petroleum and Mining Engineering Suez University Chemical Engineering and Pilot Plant Department Engineering Research Division National Research Centre Chemical and Materials Engineering Department King Abdulaziz University

In this work, new composite membranes were successfully prepared via phase inversion technique using polyvinyl chloride(PVC) and polyvinylpyrrolidone(PVP) as polymers and tetrahydrofuran(THF) and N-methyl-2-pyrrolidone(NMP) as solvents. The prepared membranes have been characterized by scanning electron microscope(SEM), and fourier transforms infrared spectroscopy(FTIR). The scanning electron microscope results prove that the prepared membranes are smooth and their pores are distributed throughout the whole surface and bulk body of the membrane without any visible cracks. The stress–strain mechanical test showed an excellent mechanical behavior enhanced by the presence of PVP in the prepared membranes. The membranes performance results showed that the salt rejection reached 98% with a high flux. This, in turn, makes the prepared membranes can be applied for sea and brackish water treatment through membrane distillation technology.

关键词： Membrane Polyvinylchloride Polyvinylpyrrolidone Phase inversion

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Comparative metabolomics of the different fractions of two saltwort(Salsola L.)species in relation to their anti-inflammatory activity

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Food Bioscience 2023年第1期51卷 937-949页

作者： Dina A.Selim Eman Shawky doaa A.ghareeb Shaymaa A.Abdulmalek Rasha M.Abu El-Khair Department of Pharmacognosy Faculty of PharmacyAlexandria UniversityEgypt Bio-screening and Preclinical Trial Lab Biochemistry DepartmentFaculty of ScienceAlexandria UniversityAlexandriaEgypt Biochemistry Department Faculty of ScienceAlexandria UniversityAlexandriaEgypt Center of Excellence for Drug Preclinical Studies(CE-DPS) Pharmaceutical and Fermentation Industry Development CenterCity of Scientific Research&Technological Applications New Borg El ArabAlexandriaEgypt College of PharmacyArab Academy for ScienceTechnology and Maritime TransportAlexandriaEgypt

Many of the plants of genus Salsola are edible halophytes which are known for their use in treatment of inflammation as well as their nutritional *** study aims at comprehensive chemical profiling of two Salsola species that are widely distributed in arid areas;*** and ***,using UHPLC-QqQ-MS in an attempt to decipher the bioactive constituents of the aerial parts of the selected plants and their ex-vivo antiinflammatory activity.73 chromatographic peaks were annotated where phytosterols dominated the metabolites in *** while phenylalkylamines were the major constituents detected in ***-DA model coefficients plot indicated that tetradecanoic acid,beta sitosterol and pentacosane were positively correlated to discrimination of *** species while N-feruloyloctopamine,N-feruloyltyramine,and ferulic acid,were positively correlated to discrimination of *** ***,fractions of ***,reduced the 5 upregulation of TNF-αcaused by LPS to levels lower than those produced by piroxicam while the petroleum ether and n-butanol fraction of *** were more effective than piroxicam in reducing IL-6 gene *** coefficients plot depicted that methyl palmitate,aegicin,cleomiscoside,beta sitosterol and sitostanol possessed strong positive correlation to the down regulation of the pro-inflammatory marker TNF-α,IL-1βand IFN-γwhile only norepinephrine derivatives were the major metabolites that showed strong positive correlation to the inhibition of IL-6 pro-inflammatory *** results obtained may help to explain chemically,the potential anti-inflammatory effect of the various Salsola species fractions increasing the value of the Salsola species as a putative functional foods.

关键词： Salsola jordanicola Salsola imbricata Chemometrics Anti-inflammatory UPLC/MS/MS Metabolomics

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Assessment of Secondary Metabolites from Marine-Derived Fungi as Antioxidant

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Open Journal of Medicinal Chemistry 2013年第3期3卷 60-73页

作者： Nihad Abdel-Monem ahmed M. Abdel-Azeem E. S. H. El Ashry doaa A. ghareeb Asmaa Nabil-Adam Department of Biochemistry Faculty of Science Alexandria University Alexandria Egypt Department of Organic Chemistry Faculty of Science Alexandria University Alexandria Egypt Laboratory of Systemic Mycology Department of Botany Faculty of Science Suez Canal University Ismailia Egypt Marine Environmental Division National Institute of Oceanography and Fisheries (NIOF) Marine Biotechnology and Natural Product (MBNP) Alexandria Egypt

Marine derived fungi are considered as a promising source of novel drugs due to their biodiversity and consequent chemo-diversity. Although marine microorganisms especially fungi are not well defined taxonomically, making this a promising frontier for the discovery of new medicines. This study focused on marine derived fungi as a model for bioactive exploration for new entities with anti-inflammatory and antioxidant capacity. Three in-vitro assays were used to investigate the bioactive antioxidant potentiality of fungal extracts. Thiobarbituric acid (TBARS),α,α-Diphenyl-β- picrylhydrazyl (DPPH) and NO assay are based on their total phenolic and flavonoid content of each extract group. Ch. globosum recorded the highest antioxidant activity (92.82%) in TBARS assay, while G. dankaliensis came first by recording 59.28% in DPPH assay in comparison with ascorbic acid (61.83%). In NO inhibition assay, N. oryzae showed 49.3% comparing with ascorbic acid (73.12%). From the preliminary result of our extracts, we can consider the marine derived fungi extracts as promising antioxidant and anti-inflammatory drug candidate.

关键词： DPPH TBARS Marine Drugs Marine Derived Fungi Anti-Inflammatory Antioxidant Capacity

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The interrelationship between insulin resistane and Alzheimer development

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Journal of Biomedical Science and Engineering 2013年第7期6卷 754-773页

作者： doaa A. ghareeb Shimaa Mohamed Mohamed El-Sayed Biochemistry Department Faculty of Science Alexandria University Alexandria Egypt

Abnormalities in insulin metabolism, characteristic of T2DM, are among the major factors thought to mechanistically influence the onset of AD. These abnormalities are thought to play a role in AD via their influence on the synthesis and degradation of Aβ and as a consequence of the cascade of neuronal alterations resulting from the effects of danger/alarm signals from oligomeric amyloid species. Additionally, recent studies have indicated that certain signal transduction pathways downstream of the InsR may also promote the generation of Aβ peptides by modulating the cleavage of the parent Aβ precursor protein (AβPP) at the γ-secretase site, a cleavage site necessary for Aβ amyloidogenicity. Glucose homeostasis is critical for energy generation, neuronal maintenance, neurogenesis, neurotransmitter regulation, cell survival and synaptic plasticity. It also plays a key role in cognitive function. In an insulin resistance condition, there is a reduced sensitivity to insulin resulting in hyperinsulinemia;this condition persists for several years before becoming full blown diabetes. Toxic levels of insulin negatively influence neuronal function and survival, and elevation of peripheral insulin concentration acutely increases its cerebrospinal fluid (CSF) concentration. Peripheral hyperinsulinemia correlates with an abnormal removal of the amyloid beta peptide (Aβ) and an increase of tau hyperphosphorylation as a result of augmented cdk5 and GSK3β activities. This leads to cellular cascades that trigger a neurodegenerative phenotype and decline in cognitive function. Chronic peripheral hyperinsulinemia results in a reduction of insulin transport across the BBB and reduced insulin signaling in brain, altering all of insulin’s actions, including its anti-apoptotic effect. However, the increase in brain insulin levels resulting from its peripheral administration at optimal doses has shown a cognition enhancing effect on patient with AD.

关键词： Insulin Resistance B-Amyloid Oxidative Stress Brain Inflammation

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Exploring the anti-inflammatory bioactive metabolites of some marine algae through integration of metabolomics,network pharmacology and molecular docking analyses

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Food Bioscience 2022年第6期50卷 861-874页

作者： Dina S.Ghallab Eman Shawky doaa A.ghareeb Shaymaa A.Abdulmalek Soad M.Mohy El-Din Mohamed M.Mohyeldin Reham S.Ibrahim Department of Pharmacognosy Faculty of PharmacyAlexandria UniversityEgypt Center of Excellency for Preclinical Study (CE-PCS) Pharmaceutical and Fermentation Industries Development CentreCity of Scientific Research and Technological ApplicationsSRTA-CityNew Borg El-Arab CityAlexandriaEgypt Bio-screening and Preclinical Trial Lab Biochemistry DepartmentFaculty of ScienceAlexandria UniversityAlexandriaEgypt Biochemistry Department Faculty of ScienceAlexandria UniversityAlexandriaEgypt Botany and Microbiology Department Faculty of ScienceAlexandria UniversityAlexandria21511Egypt

Marine algae are well-acknowledged as a prime store of largely unexplored extraordinary chemical entities potentially ameliorating against inflammation however,the efficacy mechanism remains *** study offers an integrated strategy of several bioinformatics and computational tools as well as bio-guided fractionation,and multi-step experimental validations integrated with metabolomics to systematically provide a shortcut of potentially active anti-inflammatory biomarkers of ***,multi-level network model of“multi-components-multi-targets-multiple pathways”was ***,an in vitro anti-inflammatory testing was estimated via monitoring release of pro-inflammatory ***-guided fractionation of *** integrated with UPLC-MS analysis of the most promising enriched fractions in terms of anti-inflammatory efficacy were *** docking studies on the top enriched targets were ultimately ***-level network analysis illuminated that the intricate pharmacological mechanisms underlying inflammation mitigation was the knockout of the crucial targets of TLR2,AKT1 and PRKCA and further regulation of principal pathways as PI3K-Akt signaling pathway,arachidonic acid(AA)metabolism and Toll-like receptor signaling *** vitro testing,*** exhibited the greatest ***-MS analysis of Spirulina active fractions showed linolenic acid,hydroxy linolenic acid,eicosapentaenoic acid,catechin and carnosic acid were efficacy constituents for mitigating *** docking showed that eicosapentaenoic,hydroxy linolenic acid and carnosic acid displayed favorable molecular binding capacity to TLR2,AKT1 and *** rationally developed workflow offered the convincing evidence marine algae armed with an arsenal of weapons to regain the harmony disturbed in inflammatory disorders by multi-targeted synergistic functions.

关键词： Bioinformatics Seaweeds Network pharmacology Molecular docking Metabolomics

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Evolving strategies: Enhancements in managing eosinophilic esophagitis in pediatric patients

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World Journal of Clinical Pediatrics 2024年第1期13卷 14-22页

作者： ahmed Elghoudi doaa Zourob Eman Al Atrash Fatima Alshamsi Manal Alkatheeri Hassib Narchi Rana Bitar Department of Pediatric Sheikh Khalifa Medical CityAbu DhabiUnited Arab Emirates Division of Pediatric Gastroenterology Sheikh Khalifa Medical CityAbu DhabiUnited Arab Emirates Pediatric Residency Program Sheikh Khalifa Medical CityAbu DhabiUnited Arab Emirates College of Medicine and Health Sciences United Arab Emirates UniversityAlainAbu DhabiUnited Arab Emirates

Eosinophilic esophagitis is a newly recognized disease first described about 50 years *** definition,diagnosis,and management have evolved with new published consensus guidelines and newly approved treatment available to pediatricians,enabling a better understanding of this disease and more targeted treatment for *** describe the definition,presentation,and diagnosis of eosinophilic esophagitis including management,challenges,and future directions in *** definition,diagnosis,and management of eosinophilic esophagitis have evolved over the last 50 *** guidelines and newly approved biologic treatment have enabled pediatricians to better understand this disease and allow for more targeted treatment for *** describe the definition,presentation,diagnosis,management,and treatment in addition to the challenges and future directions of eosinophilic esophagitis management in children.

关键词： Eosinophilic esophagitis Esophagitis Gastroesophageal reflux disease Food allergy Dysphagia

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Enhancing Wireless Sensor Network Efficiency through Al-Biruni Earth Radius Optimization

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Computers, Materials & Continua 2024年第6期79卷 3549-3568页

作者： Reem Ibrahim Alkanhel doaa Sami Khafaga ahmed Mohamed Zaki Marwa M.Eid Abdyalaziz A.Al-Mooneam Abdelhameed Ibrahim S.K.Towfek Department of Information Technology College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia Department of Computer Sciences College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia Computer Science and Intelligent Systems Research Center BlacksburgVirginia24060USA Delta Higher Institute for Engineering and Technology Mansoura35511Egypt Faculty of Artificial Intelligence Delta University for Science and TechnologyMansoura35712Egypt Artificial Intelligence and Computational Intelligence Research Center MeridenConnecticut06450USA School of ICT Faculty of EngineeringDesign and Information&Communications Technology(EDICT)Bahrain PolytechnicP.O.Box 33349Isa TownBahrain

The networks of wireless sensors provide the ground for a range of applications,including environmental moni-toring and industrial *** the networks can overcome obstacles like power and communication reliability and sensor coverage is the crux of network *** infrastructure planning should be focused on increasing performance,and it should be affected by the detailed data about node *** work recommends the creation of each sensor’s specs and radius of influence based on a particular geographical location,which will contribute to better network planning and *** using the ARIMA model for time series forecasting and the Al-Biruni Earth Radius algorithm for optimization,our approach bridges the gap between successive terrains while seeking the equilibrium between exploration and *** implementing adaptive protocols according to varying environments and sensor constraints,our study aspires to improve overall network *** compare the Al-Biruni Earth Radius algorithm along with Gray Wolf Optimization,Particle Swarm Optimization,Genetic Algorithms,and Whale Optimization about performance on real-world *** the most efficient in the optimization process,Biruni displays the lowest error rate at *** two other statistical techniques,like ANOVA,are also useful in discovering the factors influencing the nature of sensor data and network-specific *** to the multi-faceted support the comprehensive approach promotes,there is a chance to understand the dynamics that affect the optimization outcomes better so decisions about network design can be *** delivering better performance and reliability for various in-situ applications,this research leads to a fusion of time series forecasters and a customized optimizer algorithm.

关键词： Wireless sensor networks optimization ARIMA model BER algorithm metaheuristic algorithms

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