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Transcriptome sequencing assisted discovery and computational analysis of novel SNPs associated with flowering in Raphanus sativus in-bred lines for marker-assisted backcross breeding

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Horticulture Research 2019年第1期6卷 248-259页

作者： Jinhee kim Abinaya Manivannan do-sun kim Eun-Su Lee Hye-Eun Lee Vegetable Research Division National Institute of Horticultural and Herbal ScienceRural Development AdministrationJeonju 55365Republic of Korea

The sequencing of radish genome aids in the better understanding and tailoring of traits associated with economic *** order to accelerate the genomics assisted breeding and genetic selection,transcriptomes of 33 radish inbred lines with diverse traits were sequenced for the development of single nucleotide polymorphic(SNP)*** sequence reads ranged from 2,560,543,741 bp to 20,039,688,139 bp with the GC(%)of 47.80–49.34 and phred quality score(Q30)of 96.47–97.54%.A total of 4951 polymorphic SNPs were identified among the accessions after stringent filtering and 298 SNPs with efficient marker assisted backcross breeding(MAB)markers were generated from the polymorphic ***,functional annotations of SNPs revealed the effects and importance of the SNPs identified in the flowering *** SNPs were predominantly associated with the four major flowering related transcription factors such as MYB,MADS box(AG),AP2/EREB,and *** addition,SNPs in the vital flowering integrator gene(FT)and floral repressors(EMBRYONIC FLOWER 1,2,and FRIGIDA)were identified among the radish inbred ***,50 SNPs were randomly selected from 298 SNPs and validated using Kompetitive Allele Specific PCR genotyping system(KASP)in 102 radish inbred *** homozygosity of the inbred lines varied from 56 to 96%and the phylogenetic analysis resulted in the clustering of inbred lines into three *** together,the SNP markers identified in the present study can be utilized for the discrimination,seed purity test,and adjusting parental combinations for breeding in radish.

关键词： breeding Raphanus utilized

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CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage (Brassica rapa)

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Horticulture Research 2023年第6期10卷 135-143页

作者： Ye-Rin Lee Muhammad Irfan Siddique do-sun kim Eun Su Lee Koeun Han Sang-Gyu kim Hye-Eun Lee Vegetable Research Division National Institute of Horticultural and Herbal ScienceRural Development AdministrationWanju55365Republic of Korea Department of Horticultural Sciences North Carolina State University Mountain Horticultural Crops ResearchExtension Center 455 Research DriveMills RiverNC 28759USA Department of Biological Sciences Korea Advanced Institute for Science and TechnologyDaejeon34141Republic of Korea

Genome editing approaches,particularly the CRISPR/Cas9 technology,are becoming state-of-the-art for trait development in numerous breeding *** advances in improving plant traits are enabled by this influential tool,especially for disease resistance,compared to traditional *** of the potyviruses,the turnip mosaic virus(TuMV),is the most widespread and damaging virus that infects Brassica *** generated the targeted mutation at the eIF(iso)4E gene in the TuMV-susceptible cultivar“Seoul”using CRISPR/Cas9 to develop TuMV-resistant Chinese *** detected several heritable indel mutations in the edited T0 plants and developed T1 through generational *** was indicated in the sequence analysis of the eIF(iso)4E-edited T1 plants that the mutations were transferred to succeeding *** edited T1 plants conferred resistance to *** was shown with ELISA analysis the lack of accumulation of viral ***,we found a strong negative correlation(r=-0.938)between TuMV resistance and the genome editing frequency of eIF(iso)***,it was revealed in this study that CRISPR/Cas9 technique can expedite the breeding process to improve traits in Chinese cabbage plants.

关键词： CRISPR/Cas9 TuMV breeding

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Magnesium citrate with a single dose of sodium phosphate for colonoscopy bowel preparation

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World Journal of Gastroenterology 2011年第2期17卷 242-248页

作者： Yong sung Choi Jung Pil Suh Jong Kyu kim In Taek Lee Eui Gon Youk doo Seok Lee do sun kim doo Han Lee Department of Gastroenterology Daehang Hospital Department of Surgery Daehang Hospital

AIM:To evaluate the efficacy and acceptability of magnesium citrate and a single dose of oral sodium phosphate(45 mL) solution for morning colonoscopy bowel preparation. METHODS:A total of 159 patients were randomly assigned to receive two split doses of 90 mg of sodium phosphate(GroupⅠ,n=79) or magnesium citrate(250 mL,the day before the procedure) followed by 45 mL of sodium phosphate(the day of procedure,GroupⅡ,n= 80) .The quality of bowel cleansing and the acceptability of each regimen were compared,including the satisfaction,taste,willing to repeat and adverse effects of each regimen. RESULTS:The quality of bowel cleansing of GroupⅡ was as good as that of GroupⅠ(An Aronchick scale score of good or excellent:70.9%vs 81.0%,respectively,P=0.34;the Ottawa system score:4.4±2.6 vs 3.8 ±3.0,respectively,P=0.76) .There was no statisticallysignificant difference between both groups with regard to acceptability,including the satisfaction,taste and willingness to repeat the regimen.A significantly greater number of older patients(over 65 years old) in Group Ⅱgraded the overall satisfaction as satisfactory(48.1% vs 78.1%,respectively;GroupⅠvs GroupⅡ,P=0.01) . There were no significant adverse reactions. CONCLUSION:Magnesium citrate and a single dose of sodium phosphate was as effective and tolerable as the conventional sodium phosphate regimen and is a satisfactory option.

关键词： Colonoscopy Bowel preparation Efficacy Acceptability Magnesium citrate Sodium phosphate

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Clinical significance of distally located periurethral calcification in patients with lower urinary tract symptoms of benign prostate hyperplasia

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Asian Journal of Andrology 2023年第3期25卷 361-365页

作者： Seong Hyeon Yu do Gyeong Lim sun-Ouck kim Department of Urology Chonnam National University Medical SchoolGwang-ju 61469Korea

This study evaluated the association of periurethral calcification(PUC)with uroflowmetric parameters and symptom severity in male patients with lower urinary tract symptoms(LUTS)of benign prostatic hyperplasia(BPH).The data were collected from a prospectively maintained database of 1321 men with LUTS of BPH who visited Chonnam National University Hospital(Gwang-ju,Korea)from January 2015 to December *** severity and location were evaluated on the midsagittal plane during transrectal *** among age,prostate-related parameters,International Prostate Symptom Score(IPSS),and uroflowmetric parameters were *** the 1321 patients in this study,530(40.1%)had *** with PUC had significantly higher IPSS(mean±standard deviation[s.d.]:15.1±8.7 vs 13.1±7.9;P<0.001)and lower peak flow rate(Qmax;mean±s.d.:12.4±6.6 ml s^(-1) vs 14.7±13.3 ml s^(-1);P<0.001),compared with patients who did not have *** according to PUC severity revealed that patients with severe PUC had higher prostate-specific antigen(PSA)level(P=0.009),higher total IPSS(P<0.001),lower Qmax(P=0.002),and smaller prostate volume(P<0.001),compared with patients who had non-severe(mild or moderate)*** analysis showed that distal PUC was independently associated with high total IPSS(P=0.02),voiding symptom score(P=0.04),and storage symptom score(P=0.023),and low Qmax(P=0.015).In conclusion,PUC was significantly associated with worse LUTS parameters in terms of IPSS and ***,distally located PUC was independently associated with worse LUTS of BPH in men.

关键词： benign prostate hyperplasia calcification location

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Laser-Induced Indium-Diffusion into Cadmium Sulfide Thin Film for Solar Cell Applications

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Chinese Physics Letters 2012年第12期29卷 185-187页

作者： kim Nam-Hoon MYUNG Kuk do LEE Woo-sun Department of Electrical Engineering Chosun UniversityGwangju 501-759Republic of Korea

Laser-induced diffusion is employed to dope indium(In)into sputtering-deposited cadmium sulfide(CdS)thin *** increased optical band gap energy from 2.52 to 2.60 eV with maintenance of high optical transmittance about 60 nm in the 200-nm-thick films,the enhanced mobility over 42.5 cm^(2)/V·s,and the decreased resistivity to 1.42×10^(-3)Ω·cm are successfully obtained to be advantageous for a window layer in solar cells.

关键词： Diffusion resistivity sulfide

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Discovery of Pb-free hybrid organic-inorganic 2D perovskites using a stepwise optimization strategy

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npj Computational Materials 2022年第1期8卷 776-787页

作者： Byung do Lee Jin-Woong Lee Minseuk kim Woon Bae Park Kee-sun Sohn Department of Nanotechnology and Advanced Materials Engineering Sejong University209 Neungdong-roGwangjin-guSeoul143-747South Korea Department of Advanced Components and Materials Engineering Sunchon National UniversityChonnam57922Republic of Korea

The current status of 2D organic–inorganic hybrid perovskites for use in photovoltaic(PV)and light-emitting diode(LED)applications lags far behind their 3D ***,we propose a computational strategy for discovering novel perovskites with as few computing resources as possible.A tandem optimization algorithm consisting of an elitism-reinforced nondominated sorting genetic algorithm(NSGA-II)and a multiobjective Bayesian optimization(MOBO)algorithm was used for density functional theory(DFT)*** DFT-calculated band gap and effective mass were taken as objective functions to be optimized,and the constituent molecules and elements of a Ruddlesden–Popper(RP)structure(n=2)were taken as decision *** previously unknown RP perovskite candidates for PV and LED applications were discovered as a result of the NSGA-II/MOBO ***,more accurate DFT calculations based on the HSE06 exchange correlation functional and ab initio molecular dynamics(AIMD)were conducted for the discovered 2D perovskites to ensure their validity.

关键词： sorting optimization perovskite

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Relative importance of hydrological variables in predicting the habitat suitability of Euryale ferox Salisb.

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Journal of Plant Ecology 2018年第2期11卷 169-179页

作者： Ji Yoon kim Gu-Yeon kim Yuno do Hee-sun Park Gea-Jae Joo Department of Integrated Biological Science College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Nakdong Estuary Eco Center Busan 49435Republic of Korea

Aims Aquatic ecosystems are a priority for conservation as they have become rapidly degraded with land-use *** the habitat range of an endangered species provides crucial informa-tion for biodiversity conservation in such rapidly changing ***,the complex network structure of aquatic ecosystems restricts spatial prediction variables and has hitherto limited the use of habitat models to predict species occurrence in aquatic *** used the maximum entropy model to evaluate the potential distribution of an endangered aquatic spe-cies,Euryale ferox *** tested the relative influence of(i)climatic variables,(ii)topographic variables,and(iii)hydrological variables derived from remote sensing data to improve the predic-tion of occurrence of aquatic plant *** We considered the southern part of the Korean Peninsula as the modeling extent for the potential distribution of *** records for *** were collected from the literature and field *** applied maximum entropy modeling using remotely sensed environmental variables and evaluated their relative import-ance as prediction variables with variation *** Findings The species distribution model predicted potential habitats of *** that matched the actual distribution *** wet-lands and shallow reservoirs were the favored habitats of *** loss and fragmentation of wetland habitats appeared to be a major reason for the decrease of *** *** results also imply that hydrological variables(*** dif-ference water index)derived from remote sensing data greatly increased model prediction(relative contribution:10.5-37.0%)in the aquatic ***,interspecific competition within a similar niche environment should be considered to increase the accuracy of the distribution model.

关键词： endangered species interspecific competition NDWi remote sensing wetland

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Nontarget screening using passive air and water sampling with a level Ⅱ fugacity model to identify unregulated environmental contaminants

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Journal of Environmental Sciences 2017年第12期29卷 84-91页

作者： In-Young Chung Yu-Mi Park Hyun-Jeoung Lee Hyuk kim dong-Hoon kim Il-Gyu kim Sang-Min kim Young-sun do Kwang-Seol Seok Jung-Hwan Kwon Chemical Research Division Environmental Health Research Department National Institute of Environmental ResearchIncheon Division of Environmental Science and Ecological Engineering Korea University

It is thought that there are many unregulated anthropogenic chemicals in the *** risk assessment of chemicals, it is essential to estimate the predicted environmental concentrations. As an effort of identifying residual organic contaminants in air and water in Korea, nontarget screening using two-dimensional gas chromatography time-of-flight mass spectrometry（GC × GC-TOFMS） was conducted at 10 sites using polyurethane foam passive air sampler and at 6 sites using polydimethyl siloxane（PDMS） passive water sampler in three different seasons in 2014. More than 600 chemical peaks were identified satisfying the identification criteria in air and water samples, respectively, providing a list for further investigation. Chemical substances with reported national emission rates in2014（n = 149） were also screened for potential existence in the environment using a level Ⅱ fugacity model. Most of chemical substances classified as not detectable were not identified with detection frequency greater than 20% by nontarget screening, indicating that a simple equilibrium model has a strong potential to be used to exclude chemicals that are not likely to remain in the environment after emissions from targeted monitoring.

关键词： Nontarget screening GC × GC-TOFMS Passive sampling Pollutant release and transfer register(PRTR) Fugacity model

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Kinematic analysis of a humanoid robot CHP-1 and selection of motors in consideration of cooperative motion

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Journal of Central South University 2012年第11期19卷 3094-3099页

作者： kim do-un HWANG Sang-wook KANG Hyo-jung HONG Dae sun School of Mechatronics Changwon National UniversityChangwon 641-773Korea

A lower-part humanoid robot CHP-1 with 12 degree-of-freedom of motion has been developed for cooperative motion,such as pushing or lifting an *** capability of the robot is mainly dependent on the performance of the motors,thus the motors need to be properly *** the purpose,the kinematics of the robot was analyzed,and a number of simulations for two kinds of cooperative motions were carried *** torques required at each motor of the robot under external forces were ***,the external forces were also estimated through simulation and literature *** the basis of the torques found,the selection of motors was finally suggested,and the motors are to be installed to the humanoid robot.

关键词： humanoid robot motor selection cooperative motion kinematics

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Mechanical properties of high-strength concrete subjected to high temperature by stressed test

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中国有色金属学会会刊：英文版 2009年第B9期19卷 128-133页

作者： Gyu-Yong kim Young-sun kim Tae-Gyu LEE Division of Architecture Engineering Chun gnam National University

Recently, the effects of high temperature on compressive strength and elastic modulus of high strength concrete were experimentally investigated. The present study is aimed to study the effect of elevated temperatures ranging from 20 ℃ to 700 ℃ on the material mechanical properties of high-strength concrete of 40, 60 and 80 MPa grade. During the strength test, the specimens are subjected to a 25% of ultimate compressive strength at room temperature and sustained during heating, and when the target temperature is reached, the specimens are loaded to failure. The tests were conducted at various temperatures (20-700 ℃) for concretes made with W/B ratios of 46%, 32% and 25%, respectively. The results show that the relative values of compressive strength and elastic modulus decrease with increasing compressive strength grade of specimen.

关键词：高强混凝土高温作用力学性能测试抗压强度温度升高弹性模量强度试验

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