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OsNPF3.1,a nitrate,abscisic acid and gibberellin transporter gene,is essential for rice tillering and nitrogen utilization efficiency

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Journal of Integrative Agriculture 2024年第4期23卷 1087-1104页

作者： Junnan Hang Bowen Wu diyang qiu Guo Yang Zhongming Fang Mingyong Zhang Institute of Rice Industry Technology Research/Key Laboratory of Functional Agriculture Guizhou Provincial Department of Education/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous RegionMinistry of Education/Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province/College of Agricultural SciencesGuizhou UniversityGuiyang 550025China Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany/South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China Institute of Fruit Tree Research Guangdong Academy of Agricultural SciencesGuangzhou 510640China

Low-affinity nitrate transporter genes have been identified in subfamilies 4-8 of the rice nitrate transporter 1(NRT1)/peptide transporter family(NPF),but the OsNPF3 subfamily responsible for nitrate and phytohormone transport and rice growth and development remains *** this study,we described OsNPF3.1 as an essential nitrate and phytohormone transporter gene for rice tillering and nitrogen utilization efficiency(NUtE).OsNPF3.1 possesses four major haplotypes of its promoter sequence in 517 cultivars,and its expression is positively associated with tiller *** expression was higher in the basal part,culm,and leaf blade than in other parts of the plant,and was strongly induced by nitrate,abscisic acid(ABA)and gibberellin 3(GA_3)in the root and shoot of *** experiments demonstrated that OsNPF3.1 is a pH-dependent low-affinity nitrate transporter,with rice protoplast uptake assays showing it to be an ABA and GA_3 ***3.1 overexpression significantly promoted ABA accumulation in the roots and GA accumulation in the basal part of the plant which inhibited axillary bud outgrowth and rice tillering,especially at high nitrate *** NUtE of OsNPF3.1-overexpressing plants was enhanced under low and medium nitrate concentrations,whereas the NUtE of OsNPF3.1 clustered regularly interspaced short palindromic repeats(CRISPR)plants was increased under high nitrate *** results indicate that OsNPF3.1 transports nitrate and phytohormones in different rice tissues under different nitrate *** altered OsNPF3.1 expression improves NUtE in the OsNPF3.1-overexpressing and CRISPR lines at low and high nitrate concentrations,respectively.

关键词： rice tillering grain yield phytohormone nitrate transporter nitrogen utilization efficiency

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Peptide Transporter OsNPF8.1 Contributes to Sustainable Growth under Salt and Drought Stresses,and Grain Yield under Nitrogen Deficiency in Rice

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Rice science 2023年第2期30卷 113-126,I0031-I0034页

作者： qiu diyang HU Rui LI Ji LI Ying DING Jierong XIA Kuaifei ZHONG Xuhua FANG Zhongming ZHANG Mingyong Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement Guangdong Provincial Key Laboratory of Applied BotanySouth China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Institute of Fruit Tree Research Guangdong Academy of Agricultural SciencesGuangzhou 510640China Rice Research Institute of Guangdong Academy of Agricultural Sciences Guangzhou 510640China University of Chinese Academy of Sciences Beijing 100049China College of Agricultural Sciences Guizhou UniversityGuiyang 550025China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesGuangzhou 510650China

Peptide transport is important for plant tissues where rapid proteolysis occurs,especially during germination and senescence,to enhance redistribution of organic nitrogen(N).However,the biological role of peptide transporters is poorly investigated in *** characterized the function of the peptide transporter OsNPF8.1 of rice nitrate transporter 1/peptide transporter family(NPF).Ectopic expression of OsNPF8.1 in yeast revealed that OsNPF8.1 encoded a high-affinity di-/tri-peptide transporter,and the osnpf8.1 mutants had a lower uptake rate of the fluorescent-labelled dipeptide c in leaves of rice *** assays showed that OsNPF8.1 was highly expressed in mesophyll cells and vascular parenchyma cells,but not detected in root hairs and *** of OsNPF8.1 was induced by N deficiency,drought,Na Cl and abscisic acid,and kept at a high level in senescing *** N deficiency conditions,compared with the wild type Zhonghua 11,the osnpf8.1 mutants grew slower at the seedling stage,and had lower grain yield and lower N content in the *** contrast,OsNPF8.1-over-expressing rice(OsNPF8.1-OE)grew faster at the seedling stage and had a higher grain *** osnpf8.1 seedlings were less tolerant to salt and drought *** results suggested that stress-induced organic N transportation mediated by OsNPF8.1 might contribute to balance plant growth and tolerate to salt/drought stress and N-deficiency.

关键词： abiotic stress nitrogen peptide transporter rice

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香蕉杂交种‘粉杂1号’ABBB基因组鉴定

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园艺学报 2022年第9期49卷 1991-1997页

作者：杨兴玉许林兵吴元立邱迪洋范琳琳黄秉智广东省农业科学院果树研究所农业农村部南亚热带果树生物学与遗传资源利用重点实验室广东省热带亚热带果树研究重点实验室广州510640

香蕉杂交品种‘粉杂1号’是当前商业种植中唯一的抗香蕉枯萎病粉蕉(Musa spp. ABB,Pisang awak)品种,该品种来源于高感病的‘广粉1号’(ABB)偶然得到的种子实生苗,其基因组组成和父本未知。通过流式细胞技术(FCM)、染色体制片、基于ITS... 详细信息

香蕉杂交品种‘粉杂1号’是当前商业种植中唯一的抗香蕉枯萎病粉蕉(Musa spp. ABB,Pisang awak)品种,该品种来源于高感病的‘广粉1号’(ABB)偶然得到的种子实生苗,其基因组组成和父本未知。通过流式细胞技术(FCM)、染色体制片、基于ITS的限制性片段长度多态性聚合酶链反应(ITS-PCR-RFLP)及反转录转座子间扩增多态性(copia-IRAP)分子标记技术,并结合形态性状评分对‘粉杂1号’基因组组成进行鉴定。流式细胞分析结果显示‘粉杂1号’为四倍体,染色体制片显示染色体数为2n=4x=44条,ITS-PCR-RFLP分子标记结果显示其同时具有A和B基因组,copia-IRAP结果显示其含两套或者以上B基因组;形态打分为67分,对应基因组组成为ABBB。综合以上结果认为‘粉杂1号’基因组组成为ABBB,推测其父本可能为BB基因组组成的野生蕉。

关键词：香蕉杂交种基因组分子标记性状评分

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Methodology for credibility assessment of historical global LUCC datasets

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Science China Earth Sciences 2020年第7期63卷 1013-1025页

作者： Xiuqi FANG Wanyi ZHAO Chengpeng ZHANG diyang ZHANG Xueqiong WEI Weili qiu Yu YE Key Laboratory of Environmental Change and Natural Disaster Ministry of EducationBeijing Normal UniversityBeijing 100875China Faculty of Geographical Science Beijing Normal UniversityBeijing 100875China School of Geographical Sciences Nanjing University of Information Science and TechnologyNanjing 210044China

Land use-induced land cover change(LUCC)is an important anthropogenic driving force of global change that has influenced,and is still influencing,many aspects of regional and global *** historical global land use/cover datasets are essential for a better understanding of the impacts of LUCC on global ***,there are not only evident inconsistencies in current historical global land use/cover datasets,but inaccuracies in the data in these global dataset revealed by historical record-based reconstructed regional data throughout the world.A focus in historical LUCC and global change research relates to how the accuracy of historical global land cover datasets can be improved.A methodology for assessing the credibility of existing historical global land cover datasets that addresses temporal as well as spatial changes in the amount and distribution of land cover is therefore ***,the credibility of a global land cover dataset could be assessed by comparing similarities or differences in the data according to actual land cover data(the"true value").However,it is extremely difficult to obtain historical evidence for assessing the credibility of historical global land cover datasets,which cannot be verified through field sampling like contemporary global land cover *** proposed a methodological framework for assessing the credibility of global land cover *** the types and characteristics of the available evidence used for assessments,we outlined four methodological approaches:(1)accuracy assessment based on regional quantitative reconstructed land cover data,(2)rationality assessment based on regional historical facts,(3)rationality assessment based on expertise,and(4)likelihood assessment based on the consistency of multiple *** methods were illustrated through five case studies of credibility assessments of historical cropland cover *** framework can also be applied in assessments of oth

关键词： LUCC Global datasets Credibility Assessment methodology

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LNG动力船燃料冷能的综合开发与利用

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中国航海 2015年第3期38卷 108-111,120页

作者：李博洋张运秋邱力强李迪阳青岛远洋船员职业学院山东青岛266071 中远集团研发/技术中心北京100031 大连海事大学轮机工程学院辽宁大连116026

将中东至国内航线上的以液化天然气(Liquified Natural Gas,LNG)为动力的超大型油船(Very Large Crude Carrier,VLCC)作为母型船,介绍为其设计的LNG供气系统及利用LNG冷能的必要性,证明LNG冷能应用于船舶冷库和空调的富余量非常大。设计... 详细信息

将中东至国内航线上的以液化天然气(Liquified Natural Gas,LNG)为动力的超大型油船(Very Large Crude Carrier,VLCC)作为母型船,介绍为其设计的LNG供气系统及利用LNG冷能的必要性,证明LNG冷能应用于船舶冷库和空调的富余量非常大。设计出LNG冷能利用系统图,论证并选择合适的传热介质,提出控制方案和应急方案。计算结果表明:采用冷能利用装置后,每年可从中获得较多的直接和间接经济效益,为船舶节能减排提供了一个很好的可行方案。

关键词：船舶工程 LNG动力船 LNG燃料 LNG冷能开发与利用

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锚杆加固作用下椭圆形深埋隧洞承载力分析

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山东大学学报（工学版） 2024年第1期54卷 131-140页

作者：郝艳广徐建升明道贵雷霆邱明喜陈迪杨中交二航局建筑科技有限公司湖北武汉430050 山东科技大学土木工程与建筑学院山东青岛266590 山东大学(齐河)新材料与智能装备研究院山东德州251100

考虑施工过程中大跨度开挖、锚杆的影响等现场实际情况,分别研究椭圆形隧洞承载能力锚杆长度、锚杆数量、锚杆预应力、侧压力系数以及塑性区范围的变化规律。基于平面弹性复变函数的方法,得到围岩任意一点的应力分量表达式,结合摩尔-库... 详细信息

考虑施工过程中大跨度开挖、锚杆的影响等现场实际情况,分别研究椭圆形隧洞承载能力锚杆长度、锚杆数量、锚杆预应力、侧压力系数以及塑性区范围的变化规律。基于平面弹性复变函数的方法,得到围岩任意一点的应力分量表达式,结合摩尔-库伦(Mohr-Coulomb,MC)准则,分别得到剪判据和拉判据下的拱部承载力,讨论开挖半径以及锚杆各参数对于承载力的影响,分析不同参数对孔边应力的影响,孔边应力对形状参数更敏感,会直接影响承载力。利用3DEC软件验证了理论模型的正确性。

关键词：隧道工程椭圆形隧洞复变理论承载力分析预应力锚杆

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新型光催化反应器设计与降解甲醛性能分析

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云南化工 2021年第12期48卷 119-120,132页

作者：温惠敏丘婷婷钟建权周棣杨赵俊峰杨慧晴广东省绿色产品认证检测中心有限公司广东广州510440

采用对比法测试了不同结构折板反应器的光催化降解甲醛的效果。结果显示:折板反应器可以使反应面积和紫外光入射角增大;与平板型相比,折板反应器的平衡传质和反应效率也有所提高,90°型和45°型折板反应器的降解效率均提高1倍以上。

关键词：光催化活性甲醛反应器

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