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Involvement of ATM/ATR-p38 MAPK cascade in MNNG induced G1-S arrest

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World Journal of gastroenterology 2003年第9期9卷 2073-2077页

作者： Ke-Qing Zhu Suo-Jiang Zhang department of Pathology,School of medicine,Zhejiang University,Hangzhou 310031,Zhejiang Province,China department of Pathology School of Medicine Zhejiang University Hangzhou 310031 Zhejiang Province China.

AIM: To understand the effect of low concentration of Nmethyl-N′-nitro-nitrosoguanidine (MNNG), which is a widely distributed environmental mutagen and carcinogen especially for human gastric cancer, on DNA damage and to study its possible pathway in regulating cell cycle ***: The DNA damage effect was measured by Comet assay. A specific phospho-(Ser/Thr) ATM/ATR substrate antibody was used to detect the damage sensor by Western blot. p38 kinase activity was measured by direct kinase assay,and immunoprecipitation for the possible connection between ATM/ATR and p38 MAPK. Flow cytometry analysis and p38MAPK specific inhibitor SB203580 were combined to detect the possible cell cycle arrest by p38 ***: With the same low concentration MNNG exposure (0.2μM 2.5 h), Comet assays indicated that strand breaks accumulated, Western blot and kinase assay showed ATM/ATR and p38 kinase activated, immunoprecipitation showed phospho-ATM/ATR substrate antibody combined with both p38 MAPK antibody and phospho-p38 MAPK antibody, p38MAPK pathway was involved in the G1-S ***: Activation of ATM/ATR by MNNG induced DNA damage leads to activation of p38 MAPK, which involves in the G1 checkpoint in mammalian cells.

关键词： N-甲基-N′-硝基-N-亚硝基胍致癌物质胃癌 DNA损伤细胞周期 ATM/ATR p38

Retrorectal tumors in adults:Magnetic resonance imaging findings

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World Journal of gastroenterology 2010年第46期16卷 5822-5829页

作者： Bo-Lin Yang,Yun-Fei Gu,Wan-Jin Shao,Hong-Jin Chen,Gui-Dong Sun,department of Coloproctology,The Affiliated Hospital of Nanjing University of Chinese medicine,Nanjing 210029,China Hei-Ying Jin,department of Coloproctology,The 3rd Affiliated Hospital of Nanjing University of Chinese medicine,Nanjing 210029,China Xin Zhu,department of Radiology,The Affiliated Hospital of Nanjing University of Chinese medicine,Nanjing 210029,China department of Radiology The Affiliated Hospital of Nanjing University of Chinese Medicine No.155 Hanzhong Road Nanjing 210029 China.

AIM:To retrospectively evaluate the magnetic resonance imaging (MRI) features of adult retrorectal tumors and compare with histopathologic ***:MRI features of 21 patients with preoperative suspicion of retrorectal tumors were analyzed based on the histopathological and clinical ***:Fourteen benign cystic lesions appeared hypointense on T1-weighted images,and hyperintense on T2-weighted images with regular peripheral *** or dermoid cysts were unilocular,and tailgut cysts were *** of intracystic intermediate signal intensity was observed in one case of tailgut cyst with a component of *** solid tumors were malignant lesions and showed heterogeneous intensity on *** adenocarcinomas showed high signal intensity on T2-weighted and mesh-like enhancing areas on fat-suppressed T2-weighted *** was a fistula between the mass and anus with an internal opening in mucinous adenocarcinomas arising from anal *** stromal tumors displayed low signal intensity on T1-weighted images,and intermediate to high signal intensity on T2-weighted *** necrosis could be seen as a high signal on T2-weighted ***:MRI is a helpful technique to define the extent of the retrorectal tumor and its relationship to the surrounding structures,and also to demonstrate possible complications so as to choose the best surgical approach.

关键词： Retrorectal tumor Presacral lesions Magnetic resonance imaging Congenital cyst Malignant tumor Diagnosis

Guidelines for treatment of severe acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2002年第3期1卷 446-451页

作者： Xie-Ning Wu the department of gastroenterology. Shanghai First Municipal People’s Hospital, Shanghai 200080, China department of gastroenterology Shanghai First Municipal People's Hospital Shanghai 200080 China.

Severe acute pancreatitis is a disease of rap- id onset and progression with high mortality and morbidity. A new concept suggests that it is multifac- torially induced and involves multi-links and that therapy must be directed to several more important links at the same time. The regimen of integrated traditional Chinese and western medicine acts on the same or different sites and links to inhibit or block the inflammatory cascades. Concomitantly, preven- tive measures were taken at the start of the treatment for preventing the progression of the disease, protec- ting the function of vital organs, and avoiding the oc- currence of complications. No mortality or serious complications occurred in a series of 38 patients with Balthazar's CT grading of D and E. The treatment regimen and the mechanisms of actions of these drugs were delineated in detail in the tables and figure; the regimen is highly recommended for clinical use.

关键词： pancreatitis therapy inflammatory cytokines necrosectomy

Liver transplantation in Crigler-Najjar syndrome type I disease

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Hepatobiliary & Pancreatic Diseases International 2012年第5期11卷 545-548页

作者： Zhen-Hua Tu, De-Sheng Shang, Jin-Cai Jiang, Wu Zhang, Min Zhang, Wei-Lin Wang, Hai-Yan Lou,Shu-Sen Zheng division of Hepatobiliary and Pancreatic Surgery, department of Surgery, First Affiliated Hospital, Zhejiang University School of medicine, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health & Key Laboratory of Organ Transplantation of Zhejiang Province department of Radiology,First Affiliated Hospital,Zhejiang University School of medicine, Hangzhou 310003, China division of Hepatobiliary and Pancreatic Surgery Department of Surgery First Affiliated Hospital Zhejiang University School of Medicine Key Laboratory of Combined Multi-Organ Transplantation Ministry of Public Health & Key Laboratory of Organ Transplantation of Zhejiang Province Hangzhou 310003 Hangzhou China department of Radiology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 Hangzhou China

BACKGROUND: Crigler-Najjar syndrome type I (CNS I) is a very rare autosomal recessive inherited disease that liver transplantation can properly deal with. METHODS: We present one case of an 18-month-old child with CNS I diagnosed by clinical findings and genetic detecting LTx was performed 5 days after kernicterus broke out and neurological symptoms were successfully reversed. RESULT: Magnetic resonance imaging and magnetic resonance spectroscopy showed encouraging results that brain pathology had a trend to return to normal in 1-year follow-up, combined with electroencephalogram and motor development estimate studies. CONCLUSIONS: Liver transplantation can cure CNS I with reversible neurological symptoms to some extent in time Magnetic resonance spectroscopy may be a future option of predicting brain conditions and selecting suitable patients with CNS I for transplantation.

关键词： Crigler-Najjar liver transplantation inborn error of metabolism magnetic resonance imaging hyperbilirubinemia

维甲酸对人胃癌细胞HGC-27增殖、细胞周期及细胞周期素依赖性激酶抑制蛋白(P16、P21、P27)表达的影响

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复旦学报（医学版） 2002年第6期29卷 428-432页

作者：崔建华李华范钰林庚金钱立平徐康颜吉丽南通医学院附属东台医院消化科东台224200 复旦大学医学院病理学教研室上海200032 复旦大学附属华山医院消化科上海200040 南京中医药大学第一临床医学院内科学教研室南京210029

目的　观察维甲酸 (RA)作用人胃癌细胞系GHC - 2 7后 ,细胞增殖、细胞周期及细胞周期素依赖性激酶抑制蛋白 (CKIs) (P16、P2 1、P2 7)表达的变化 ,探讨RA抑制HGC 2 7增殖 ,促进其分化的作用机制。方法　不同浓度RA作用HGC 2 7细胞后 ,MT... 详细信息

目的　观察维甲酸 (RA)作用人胃癌细胞系GHC - 2 7后 ,细胞增殖、细胞周期及细胞周期素依赖性激酶抑制蛋白 (CKIs) (P16、P2 1、P2 7)表达的变化 ,探讨RA抑制HGC 2 7增殖 ,促进其分化的作用机制。方法　不同浓度RA作用HGC 2 7细胞后 ,MTT法检测细胞增殖情况 ,流式细胞仪检测细胞周期 ,Westermblot、Northernblot分别检测CKIs(P16、P2 1、P2 7)蛋白、mRNA水平。结果　在一定浓度范围之内 ,RA能抑制HGC 2 7细胞增殖 ,细胞周期发生G1→S期阻滞 ,并上调P2 1、P2 7的蛋白、mRNA水平 ,但对P16的蛋白、mRNA水平没有影响。结论　RA能通过上调CKIs尤其是P2 1、P2 7的表达而抑制HGC 2 7增殖 ,促进其分化。

关键词：维甲酸胃癌细胞 HGC-27 细胞周期细胞周期素依赖性激酶抑制蛋白细胞增殖

Alcoholism and alcoholic liver disease: focusing on epidemiological investigation in Asia

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Hepatobiliary & Pancreatic Diseases International 2005年第2期4卷 170-172页

作者： You-Ming Li Hangzhou, China department of gastroenterology, First Affiliated Hos- pital, Zhejiang University School of medicine, Hangzhou 310003, China

p16基因启动子CpG区甲基化与人脑胶质瘤临床病理特征的关系

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复旦学报（医学版） 2004年第5期31卷 477-480页

作者：罗艺陈坚吕元复旦大学附属华山医院检验医学中心上海200040 复旦大学附属华山医院消化科上海200040

目的　研究 p16基因启动子CpG区甲基化的改变与脑胶质瘤发生及其临床病理特征的关系。方法　采用甲基化特异PCR技术 (MSP法 )研究脑胶质瘤组织p16基因甲基化 ,并分析其与临床病理特征的关系。结果　 5 6例脑胶质瘤组织中有 10例 (17.9... 详细信息

目的　研究 p16基因启动子CpG区甲基化的改变与脑胶质瘤发生及其临床病理特征的关系。方法　采用甲基化特异PCR技术 (MSP法 )研究脑胶质瘤组织p16基因甲基化 ,并分析其与临床病理特征的关系。结果　 5 6例脑胶质瘤组织中有 10例 (17.9% )呈 p16CpG区甲基化阳性。胶质瘤的 p16CpG区甲基化与性别、年龄无关 ,而与肿瘤级别、浸润与否有关。有浸润的脑胶质瘤 p16CpG区甲基化明显高于暂无浸润者。p16CpG区甲基化也多发生于Ⅲ级、Ⅳ级 (即肿瘤恶性程度高 )的病例。

关键词：甲基化人脑胶质瘤临床病理特征 p16基因瘤组织肿瘤启动子 PCR技术影响因子

Heterologous expression of human cytochrome P450 2E1 in HepG2 cell line

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World Journal of gastroenterology 2003年第12期9卷 2732-2736页

作者： Jian Zhuge Ye Luo Ying-Nian Yu department of Pathophysiology,School of medicine,Zhejiang University,Hangzhou 310031,Zhejiang Province,China department of Pathophysiology School of Medicine Zhejiang University Hangzhou 310031 Zhejiang Province China.

AIM: Human cytochrome P-450 2E1 (CYP2E1) takes part in the biotransformation of ethanol, acetone, many smallmolecule substrates and volatile anesthetics. CYP2E1 is involved in chemical activation of many carcinogens,procarcinogens, and toxicants. To assess the metabolic and toxicological characteristics of CYP2E1, we cloned CYP2E1 cDNA and established a HepG2 cell line stably expressing recombinant CYP ***: Human CYP2E1 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR)from total RNAs extracted from human liver and cloned into pGEM-T vector. The cDNA segment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant plasmid of pREP9-CYP2E1 to HepG2 cells. The expression of CYP2E1 mRNA was validated by RT-PCR. The enzyme activity of CYP2E1 catalyzing oxidation of 4-nitrophenol in postmitochondrial supernate (S9) fraction of the cells was determined by spectrophotometry. The metabolic activation of HepG2-CYP2E1 cells was assayed by N-nitrosodiethylamine (NDEA)cytotoxicity and micronucleus ***: The cloned CYP2E1 cDNA segment was identical to that reported by Umeno et al(GenBank access No.J02843). HepG2-CYP2E1 cells expressed CYP2E1 mRNA and had 4-nitrophenol hydroxylase activity (0.162±***-1 S9 protein), which were undetectable in parent HepG2 cells. HepG2-CYP2E1 cells increased the cytotoxicity and micronucleus rate of NDEA in comparison with those of HepG2 ***: The cDNA of human CYP2E1 can be successfully cloned, and a cell line, HepG2-CYP2E1, which can efficiently express mRNA and has CYP2E1 activity, is established. The cell line is useful for testing the cytotoxicity,mutagenicity and metabolism of xenobiotics, which may possibly be activated or metabolized by CYP2E1.

关键词： CYP2E1 生物转化 HepG2细胞 RT-PCR 克隆 pGEM-T 肝组织

Clinicopathological features of gastric glomus tumor

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World Journal of gastroenterology 2010年第36期16卷 4616-4620页

作者： Hui-Qiong Fang,Jing Yang,Fen-Fen Zhang,An-Jia Han,department of Pathology,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,Guangdong Province,China Hui-Qiong Fang,department of Pathology,Foshan Hospital of Traditional Chinese medicine,Foshan 528000,Guangdong Province,China Jing Yang,department of Pathology,People’s Hospital of Zhu-hai City,Zhuhai 519000,Guangdong Province,China Yi Cui,department of gastroenterology,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,Guangdong Province,China department of Pathology First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China.

AIM:To study the clinicopathological features of gastric glomus tumor and review the related Chinese literature published in ***:A case of gastric glomus tumor was *** findings in 56 cases of gastric glomus tumor were ***:Gastric glomus tumor was far more common in women than in men with a female to male ratio of 1.6:*** median age of the patients was 45 years(range 28-79 years).The patients often complained of epigastric pain and bloody *** tumor was located in antrum of the *** greatest diameter of the tumor was ***,the tumor was comprised of nests of glomus cells surrounding the *** cells were small,uniform and ***,smooth muscle actin and actin were expressed in the *** markers,including S-100 protein,CD34,CD117,desmin,CD56,synaptophysin,chromo-granin A,neuron specific enolase and cytokeratin were all ***:Gastric glomus tumor is a rare benign mesenchymal *** diagnosis depends on pathologic *** diagnosis includes gastrointestinal stromal tumor,paraganglioma and carcinoid tumor.

关键词： Stomach Glomus tumor Clinicopathology Immunohistochemistry