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Soil boron fertilization:The role of nutrient sources and rootstocks in citrus production

Journal of Integrative Agriculture 2017年第7期16卷 1609-1616页

作者： dirceu mattos jr Franz W R Hippler Rodrigo M Boaretto Eduardo S Stuchi Jose A Quaggio Centro de Citricultura Sylvio Moreira Instituto AgronSmicoCordeiropolis(SP)13490-970Brazil Estacao Experimental de Citricultura de Bebedouro Bebedouro(SP)14700-970Brazil Centro de Solos e Recursos Ambientais Instituto Agronomico/Bara de ItapuraCampinas(SP)13020-902Brazil

Boron （B） is a key element for citrus production, especially in tropical regions, where the nutrient availability is commonly low in the soil. In addition, information about doses, fertilizer sources, methods of application, and particularly, differential nutrient demand of scion/rootstock combinations are required for efficient fertilization of commercial groves. In a non-irri- gated sweet orange orchard （cv. Natal）, grafted onto Rangpur lime, Swingle citrumelo or Sunki mandarin, we studied the application of two sources of B： boric acid （17% B, soluble in water） and ulexite （12% B, partiaJly soluble in water） at four levels of supply （control without B, and soil application of 2, 4 and 6 kg ha-1 yr-1 of B）. The experiment was carried out for three years （2004-2006）. Boron availability in the soil and concentration in the leaves, as well as the fruit yield and quality of trees were evaluated. Soil B extracted with hot water and total leaf B positively correlated with doses of the nutrient applied to the trees. Levels of B in the soil and in the leaves did not vary with fertilizer sources. Fruit yield of trees grafted onto Rangpur lime and Swingle citrumelo was more responsive to B doses than those grafted onto Sunki mandarin. The maximum fruit yield of trees grafted onto Swingle was obtained with 3.2 kg ha-1 yr-1 of B, and leaf B level of 280 mg kg-1 that point out to a highest demand for B when this combination was compared with other rootstocks. Furthermore, fertilization with B did not affect the quality of fruits, but correlated with B and potassium （K） concentrations in the leaves. These results also support that the current recommendations for levels of B in leaves should be revisited.

关键词： boric acid ulexite fertilization via soil leaf analysis fruit quality citrus

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Fluoride Exposure Compromises Gas Exchange of Plants

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American Journal of Plant Sciences 2013年第12期4卷 16-20页

作者： Geisa Lima Mesquita Eduardo Caruso Machado Ricardo Machado Heitor Cantarella dirceu mattos jr. Centro de Citricultura Sylvio Moreira (IAC) Cordeirópolis Brazil Centro de Ecofisiologia e Biofísica (IAC) Campinas Brazil Centro de Solos e Recursos Ambientais (IAC) Campinas Brazil Centro de Citricultura Sylvio Moreira (IAC) Cordeirópolis Brazil

Fluorine (F-) stands out for its phytotoxic potential, because it accumulates in plants, changes enzymes activity, reduces chlorophyll content and, consequently, affects growth and yield of crop plants. An experiment was conducted to evaluate the effects of F- on leaf gas exchange in coffee and sweet orange plants, compared to sensitive (gladiolus) and tolerant (ryegrass) reference species. Plants grown in pots were exposed to F- in a semi-open mist chamber. The experimental design was completely randomized with treatments defined by the combination of plant species and two intensities of exposure to atmospheric F, with nebulization of HF solutions (low = 0.065 mmol·m-3 and high = 0.260 mmol·m-3) in a mist chamber, as well as with non-exposed control samples. CO2 assimilation (A), transpiration (E), stomatal conductance (gs) and chlorophyll fluorescence rates were measured after 27 days of treatment application. The leaf gas exchange variables in ryegrass and orange plants did not vary in response to the increase in atmospheric F, while an increase in gs and E values was observed in gladiolus and coffee plants. A decrease in A and potential quantum efficiency of photosystem II (Fv/Fm) was found for gladiolus plants. On the contrary, an increase of A for coffee plants was associated with the apparent effect previously reported about the loss of leaf stomatal regulation related to the short assessment period of plants in this experiment. Damages caused to the photosynthetic system were reflected in the susceptibility of the evaluated species to the contamination by the element.

关键词： Fluoride Pollution Gas Exchange Citrus Coffee Bioindicator Species

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Liver transplantation and alcoholic liver disease:History,controversies,and considerations

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World Journal of Gastroenterology 2018年第26期24卷 2785-2805页

作者： Claudio Augusto Marroni Alfeu de Medeiros Fleck jr Sabrina Alves Fernandes Lucas Homercher Galant Marcos Mucenic Mario Henrique de mattos Meine Guilherme Mariante-Neto Ajacio Bandeira de Mello Brandao Graduate Program in Medicine:Hepatology Universidade Federal de Ciências da Saúde de Porto Alegre(UFCSPA)Porto Alegre 90430-080RSBrazil Liver Transplant Adult Group Irmandade da Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90035-072RSBrazil Nutrition at the Centro Universitário Metodista(IPA) Porto Alegre 90420-060RSBrazil

Alcohol consumption accounts for 3.8% of annual global mortality worldwide, and the majority of these deaths are due to alcoholic liver disease(ALD), mainly alcoholic cirrhosis. ALD is one of the most common indications for liver transplantation(LT). However, it remains a complicated topic on both medical and ethical grounds, as it is seen by many as a "self-inflicted disease". One of the strongest ethical arguments against LT for ALD is the probability of relapse. However, ALD remains a common indication for LT worldwide. For a patient to be placed on an LT waiting list, 6 mo of abstinence must have been achieved for most LT centers. However, this "6-mo rule" is an arbitrary threshold and has never been shown to affect survival, sobriety, or other outcomes. Recent studies have shown similar survival rates among individuals who undergo LT for ALD and those who undergo LT for other chronic causes of end-stage liver disease. There are specific factors that should be addressed when evaluating LT patients with ALD because these patients commonly have a high prevalence of multisystem alcohol-related changes. Risk factors for relapse include the presence of anxiety or depressive disorders, short pre-LT duration of sobriety, and lack of social support. Identification of risk factors and strengthening of the social support system may decrease relapse among these patients. Family counseling for LT candidates is highly encouraged to prevent alcohol consumption relapse. Relapse has been associated with unique histopathological changes, graft damage, graft loss, and even decreased survival in some studies. Research has demonstrated the importance of a multidisciplinary evaluation of LT candidates. Complete abstinence should be attempted to overcome addiction issues and to allow spontaneous liver recovery. Abstinence is the cornerstone of ALD therapy. Psychotherapies, including 12-step facilitation therapy, cognitive-behavioral therapy, and motivational enhancement therapy, help

关键词： Alcoholic liver disease Alcoholic hepatitis Alcoholic cirrhosis Alcoholism Liver transplantation Alcoholic recurrence Controversies Alcoholic abstinence Relapse Selection criteria

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Plasmid instability when the hsp60 gene promoter is used to express the protective non-toxic fragment B of the diphtheria toxin in recombinant BCG

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American Journal of Molecular Biology 2013年第2期3卷 81-86页

作者： Dilzamar V.Nascimento Odir A.Dellagostin Raphael Hirata jr Geraldo M.B.Pereira Ana Luiza mattos-Guaraldi Geraldo R.Garcia Armoa Faculdade de Ciencias Médicas Universidade do Estado do Rio de JaneiroRio de JaneiroBrazil Centro de Biotecnologia Universidade Federal de PelotasCampus UniversitárioPelotasBrazil Instituto Oswaldo Cruz Fundacao Oswaldo CruzRio de JaneiroBrazil

The genetic modification of the live attenuated Mycobacterium bovis BCG to deliver a protective Corynebacterium diphtheriae antigen in vivo could be a safer and less costly alternative to the new and more expensive DTP vaccines available today, in particular to third world-countries. The stability of expression of heterologous antigens in BCG, however, is a major challenge to the use of live recombinant bacteria in vaccine development and appears to be dependent to a certain extent, on a genetic compatibility between the expression cassette within the plasmid construct and the mycobacterium host. In the quest for the best recombinant BCG transformant to express the dtb gene of C. diphtheriae we generated two new rBCG strains by transforming the Moreau substrain of BCG with the mycobacterial expression vectors pUS973 and pUS977, each one carrying a different promoter to drive the expression of the target antigen. After transformation recombinant BCG clones were selected on Middlebrook 7H10 kanamycin Agar plates, expanded in Middlebrook 7H9 kanamycin Broth and analyzed by agarose gel electrophoresis and immunoblotting. rBCGs transformed with the construct carrying the weak PAN promoter from M. paratuberculosis stably expressed the dtb gene. Conversely, rBCGs transformed with the construct carrying the strong mycobacterium hsp60 promoter were unstable and consequently unfit for the expression of the C. diphtheriae gene.

关键词： BCG Vaccine Recombinant BCG Fragment B of Diphtheria Toxin Anti-Diphtheria Vaccine

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Comparison between the Use of Loose and Stranded Seeds in Prostate Brachytherapy in Brazil

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Open Journal of Urology 2012年第3期2卷 206-209页

作者： Fernando SPeleias jr. Carlos A.Zeituni Elisa C.M.Rostelato Carla D.Souza Fabio R.mattos Marcos A.G.Benega Nuclear and Energy Research Institute(IPEN/CNEN-SP) São PauloBrazil

Cancer is a leading cause of death worldwide. In 2030, 26.4 million new cases will be diagnosed, with 17 million deaths accounted worldwide. Prostate cancer is the sixth most common type in the world, and the second most commom in men. For Brazil, the number of new cases of prostate cancer in 2010 is estimated to 52,350. Treatment of prostate cancer may be by surgery, radiation or even vigilant observation. A method of radiotherapy which has been extensively used is brachytherapy, where Iodine-125 seeds are placed inside or next to the area requiring treatment. Iodine seeds can be introduced loose seeds or stranded in bioabsorbable polymers in order to increase the dosimetric coverage of the prostate and reducing the chance of seed migration. Data were gathered concerning the commercialization of loose seeds and stranded seeds, between 2005 and 2011, in Brazil. It was noted that the number of stranded seeds commercialized in Brazil (around 80%) has always been much greater than the number of loose seeds, reaching 90% last year. The main reason is the reduction of the seed migration events into other parts of the body, since there is a potential hazard even considering that no harmful effect has been observe. The data regarding dosimetry are still controversial, since the seeds that migrate is normally no more than 1%.

关键词： Cancer Prostate Brachytherapy Iodine-125 Seeds Loose Seeds Stranded Seeds

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Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin

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American Journal of Molecular Biology 2017年第4期7卷 176-189页

作者： Dilzamar V.Nascimento Odir A.Dellagostin Denise C.S.Matos Douglas McIntosh Raphael Hirata jr. Geraldo M.B.Pereira Ana Luíza mattos-Guaraldi Geraldo R.G.Armoa Faculdade de Ciências Médicas Universidade do Estado do Rio de JaneiroRio de JaneiroBrazil Núcleo de Biotecnologia Universidade Federal de PelotasCampus UniversitárioPelotasBrazil Instituto de Tecnologia em Imunobiológicos Fundacao Oswaldo CruzRio de JaneiroBrazil Instituto Oswaldo Cruz Fundacao Oswaldo CruzRio de JaneiroBrazil Instituto de Veterinária Universidade Federal Rural do Rio de JaneiroSeropedicaRio de JaneiroBrazil

Diphtheria is a fulminant bacterial disease caused by toxigenic strains of Corynebacterium diphtheriae whose local and systemic manifestations are due to the action of the diphtheria toxin (DT). The vaccine which is used to prevent diphtheria worldwide is a toxoid obtained by detoxifying DT. Although associated with high efficacy in the prevention of disease, the current anti-diphtheria vaccine, one of the components of DTP (diphtheria, tetanus and pertussis triple vaccine), may present post vaccination effects such as toxicity and reactogenicity resulting from the presence of contaminants in the vaccine that originated during the process of production and/or detoxification. Therefore, strategies to develop a less toxic and at the same time economically viable vaccine alternatives are needed to improve existing vaccines in use worldwide. In this study, the Moreau substrain of BCG which is used in Brazil as a live vaccine against human tuberculosis was genetically modified to carry and express the gene encoding for the diphtheria toxin fragment B (DTB). As such, the DNA sequence encoding the dtb gene was cloned into the pUS977 shuttle vector for cytoplasmic expression and successfully introduced into BCG cells by electroporation. Mice immunized with recombinant BCG expressing DTB showed seroconversion with the detection of specific antibodies against DTB. Also, rBCGs stably expressing DTB persisted up to 60 days in the absence of selective pressure in mice and cell viability did not change significantly during the period tested. Finally, immune sera from BALB/c mice vaccinated with rBCGpUS977dtbPW8 were preliminarily tested for their capacity of neutralizing the diphtheria toxin in the Vero Cells assay.

关键词： Recombinant BCG Diphtheria Toxin dtb Gene Park Williams 8(PW8) Corynebacterium diphtheriae rDTBPW8 pUS977 Vector

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