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Overexpression of the stress-induced OsWRKY08 improves osmotic stress tolerance in Arabidopsis

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Chinese Science Bulletin 2009年第24期54卷 4671-4678页

作者： SONG yu JING ShaoJuan yu diqiu Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Kunming 650223 China Graduate School of Chinese Academy of Sciences Beijing 100039 China

Previous Northern blotting analyses of rice seedlings have screened several WRKY genes among the transcripts that are differentially regulated in the following treatments: high salinity, cold stress, polyethylene glycol (PEG) and heat shock. Here, we report characterization of a WRKY gene, OsWRKY08, in rice, which was found to be inducible by PEG, NaCl, Abscisic acid (ABA), and naphthalene acetic acid (NAA) as its ortholog AtWRKY28 in Arabidopsis. To determine whether overexpression of OsWRKY08 alters abiotic stress tolerance, 35S::OsWRKY08 recombinant was generated and transformed into Arabidopsis. Physiological tests indicated that 35S::OsWRKY08 transgenic Arabidopsis displayed increased tolerance to mannitol stress through increasing the lateral root number and primary root length during seeding root development. Further, semi-quantitative RT-PCR showed that AtCOR47 and AtRD21, two ABA-independent abiotic stress responded genes, were induced in 35S::OsWRKY08 transgenic plants. These results suggest OsWRKY08 improves the osmotic stress tolerance of transgenic Arabidopsis through an ABA-independent signaling pathway.

关键词：转基因拟南芥渗透胁迫应力诱发 Northern杂交 35S启动子非生物胁迫水稻幼苗 WRKY

Trehalose phosphate synthase 5-dependent trehalose metabolism modulates basal defense responses in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2019年第4期61卷 509-527页

作者： Xuelan Wang Yan Du diqiu yu Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences University of Chinese Academy of Sciences

Despite the recent discovery that trehalose synthesis is important for plant development and abiotic stress tolerance, the effects of trehalose on biotic stress responses remain relatively unknown. In this study, we demonstrate that TREHALOSE PHOSPHATE SYNTHASE 5(TPS5)-dependent trehalose metabolism regulates Arabidopsis thaliana defenses against pathogens(necrotrophic Botrytis cinerea and biotrophic Pseudomonas syringae).Pathogen infection increased trehalose levels and upregulated TPS5 expression. Application of exogenous trehalose significantly improved plant defenses against B. cinerea, but increased the susceptibility of plants to P. syringae. We demonstrate that elevated trehalose biosynthesis, in transgenic plants over-expressing TPS5, also increased the susceptibility to P. syringae, but decreased the disease elcit r symptoms caused by B. cinerea. The knockout of TPS5 prevented the accumulation of trehalose and enhanced defense responses against P. syringae. Additionally, we observed that a TPS5-interacting protein(multiprotein bridging factor 1c) was required for induced expression of TPS5 during pathogen infections. Furthermore, we show that trehalose promotes P. syringae growth and disease development, via a mechanism involving suppression of the plant defense gene, Pathogenesis-Related Protein 1. These findings provide insight into the function of TPS5-dependent trehalose metabolism in plant basal defense responses.

关键词： Trehalose phosphate synthase Arabidopsis thaliana^FA

WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age-mediated flowering

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Journal of Integrative Plant Biology 2020年第11期62卷 1659-1673页

作者： Zhenbing Ma Wei Li Houping Wang diqiu yu School of Life Sciences University of Science and Technology of ChinaHefeiAnhui 230027China CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical Gardenthe Chinese Academy of SciencesKunming 650223China State Key Laboratory for Conservation and Utilization of Bio-Resources in yunnan Yunnan UniversityKunming 650091China School of Horticulture Anhui Agricultural UniversityHefei 230036China

WRKY12 and WRKY13 are two WRKY transcription factors that play important roles in the control of flowering time under short-day(SD)*** temporally regulated expression of WRKY12 and WRKY13 indicates that they may be involved in the age-mediated flowering ***,their roles in this pathway are poorly ***,we show that the transcription of WRKY12 and WRKY13 is directly regulated by SQUAMOSA PROMOTER BINDING-LIKE 10(SPLio),a transcription factor downstream of the age *** and activation analyses revealed that SPL10 functions as a positive regulator of WRKY12 and a negative regulator of *** mechanistic investigation revealed that WRKY12 and WRKY13 physically interact with SPL10 and that both of them bind to the promoter of ***,the WRKY12-SPL10 and WRKY13-SPL10 interactions facilitate and inhibit SPL10 transcriptional function,respectively,to regulate miRiy2b ***,our results show that WRKY12 and WRKY13 participate in the control of age-mediated flowering under SD conditions though physically interacting with SPLs and co-regulating the target gene miRi72b.

关键词： expression. WRKY SPL

Functional characterization of the Arabidopsis SERRATE under salt stress

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Plant Diversity 2021年第1期43卷 71-77页

作者： Minghui Mou Qijuan Wang Yanli Chen diqiu yu Ligang Chen CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunMenglaYunnan 666303China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan 666303China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

SERRATE(SE)plays critical roles in RNA metabolism and plant growth ***,its function in stresseresponse processes remains largely ***,we examined the regulatory role of SE using the se-1 mutant and its complementation line under saline *** expression of SE was repressed by salt treatment at both mRNA and protein *** treatment with different NaCl concentrations,the se-1 mutants showed increased sensitivity to *** heightened sensitivity was evidenced by decreased germination,reduced root growth,more serious chlorosis,and increased conductivity of the mutants compared with the wild *** analysis revealed that SE regulates the pre-mRNA splicing of several well-characterized marker genes associated with salt stress *** data thus imply that SE may function as a key component in plant response to salt stress by modulating the splicing of salt stress-associated genes.

关键词： SERRATE Salt stress Pre-mRNA alternative splicing

bHLH104 confers tolerance to cadmium stress in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2018年第8期60卷 691-702页

作者： Xiani Yao yuerong Cai diqiu yu Gang Liang Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Kunming 650223 China University of Chinese Academy of Sciences Beijing 100049 China

Cd is a non-essential heavy metal that is toxic to both plants and animals. Here, we reveal that the transcription factor bHLH104 positively regulates Cd tolerance in Arabidopsis thaliana. We show that Fe deficiency-responsive genes were induced by Cd treat- ment, and that their upregulation was suppressed in bhlh104 loss-of-function mutants, but enhanced upon overexpression of bHLH104. Correspondingly, the bhlh104 mutants displayed sensitivity to Cd stress, whereas plants overexpressing bHLH104 exhibited enhanced Cd tolerance. Further analysis suggested that bHLH104 positivelyregulates four heavy metal detoxification-associated genes, IREG2, A4TP3, HA4A3 and NAS4, which play roles in Cd sequestration and tolerance. The bHLH104 overexpres- sion plants accumulated high levels of Cd in the root but low levels of Cd in the shoot, which might contribute to the Cd tolerance in those lines. The present study thus points to bHLH104 as a potentially useful tool for genetic engineering of plants with enhanced Cd tolerance.

关键词： bHLH104 confers tolerance to cadmium stress in Arabidopsis thaliana Cd Fe

ERF1 delays flowering through direct inhibition of FLOWERING LOCUS T expression in Arabidopsis

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Journal of Integrative Plant Biology 2021年第10期63卷 1712-1723页

作者： Yanli Chen Liping Zhang Haiyan Zhang Ligang Chen diqiu yu CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMengla 666303China State Key Laboratory for Conservation and Utilization of Bio-Resources in yunnan Yunnan UniversityKunming 650091China

ETHYLENE RESPONSE FACTOR1(ERF1)is a key component in ethylene signaling,playing crucial roles in both biotic and abiotic stress ***,we demonstrate that ERF1 also has an important role during floral initiation in Arabidopsis *** or knockout of ERF1 accelerated floral initiation,whereas overexpression of ERF1 dramatically delayed floral *** contrasting phenotypes were correlated with opposite transcript levels of FLOWERING LOCUS T(FT).Chromatin immunoprecipitation(ChIP)assays revealed that ERF1 associates with genomic regions of the FT gene to repress its ***-10/ERF1RNAi plants showed a similar flowering phenotype to the ft-10 mutant,whereas the flowering of FTox/ERF1ox mimicked that of FTox plants,suggesting that ERF1 acts upstream of FT during floral ***,altered floral transition in ethylene-related mutants was also correlated with FT *** analysis suggested that ERF1 also participates in delay in flowering-time control mediated by the ethylene precursor 1-aminocyclopropane-1-carboxylic ***,ERF1 may act as a negative modulator of flowering-time control by repressing FT transcription in Arabidopsis.

关键词： Arabidopsis thaliana ethylene ETHYLENE RESPONSE FACTOR1 floral initiation FLOWERING LOCUS T

Phytochrome B regulates jasmonic acid-mediated defense response against Botrytis cinerea in Arabidopsis

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Plant Diversity 2022年第1期44卷 109-115页

作者： Shengyuan Xiang Songguo Wu Yifen Jing Ligang Chen diqiu yu CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunMenglaYunnan666303China University of Chinese Academy of Sciences Beijing100049China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan666303China Laboratory for Conservation and Utilization of Bio-Resources in yunnan Yunnan UniversityKunmingYunnan666303China

The phytochrome B mediated light signaling integrates with various phytohormone signalings to control plant immune ***,it is still unclear whether phyB-mediated light signaling has an effect on the biosynthesis of jasmonate during plant defense response against Botrytis *** this study,we demonstrated that phyB-mediated light signaling has a role in this ***,we confirmed that phyb plants were obviously less resistant to *** while phyB overexpressing plants showed significantly enhanced *** also found that the expression of numerous JA biosynthesis genes was promoted upon treatment with red or white light when compared to that of darkness,and that this promotion is dependent on *** with the gene expression results,phyb plants accumulated reduced pool of JA-lle,indicating that phyB-mediated light signaling indeed increased JA *** genetic analysis showed that light-mediated JAZ9 degradation and phyB-enhanced resistance were dependent on the receptor COI1,and that pifl/3/4/5(pi/q)can largely rescue the severe symptom of *** together,our study demonstrates that phyB may participate in plant defense against *** through the modulation of the biosynthesis of JA.

关键词： phyB Botrytis cinerea JA biosynthesis COI1 JAZ PIF

Mapping and breeding value evaluation of a semi-dominant semidwarf gene in upland rice

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Plant Diversity 2018年第5期40卷 238-244页

作者： Xiaoqian Chen Peng Xu Jiawu Zhou Dayun Tao diqiu yu Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences KunmingYunnan. 650223 China University of Chinese Academy of Sciences Beijing 100049 China Food Crops Research Institute Yunnan Academy of Agricultural Sciences (YAAS) Kunming 650200 China

Plant height is an important trait related to yield potential and plant architecture. A suitable plant height plays a crucial role in improvement of rice yield and lodging resistance. In this study, we found that the traditional upland landrace ＇Kaowenghan＇（KWH） showed a special semi-dwarf phenotype. To identify the semi-dwarf gene from KWH, we raised BC2F4 semi-dwarf introgression lines （IL） by hybridization of the japonica rice cultivar ＇Dianjingyoul＇（DJY1） and KWH in a DJY1 background. The plant height of the homozygous semi-dwarf IL （IL-87） was significantly reduced compared with that of DJY1. The phenotype of the F1 progeny of the semi-dwarf IL-87 and DJY1 showed that the semi-dwarf phenotype was semi- dominant. QTL mapping indicated that the semi-dwarf phenotype was controlled by a major QTL qDH1 and was localized between the markers RM6696 and RM12047 on chromosome 1. We also developed near-isogenic lines （NIL） from the BC3F3 population, and found that the yield of homozygous NIL （NIL-2） was not significantly different compared to DJY1. Breeding value evaluation through investigation of the plant height of the progeny of NIL （NIL-2） and cultivars from different genetic background indicate that the novel semi-dwarf gene shows potential as a genetic resource for rice breeding.

关键词： Semi-dwarf QTL mapping Breeding value evaluation

AtWRKY75 positively regulates age-triggered leaf senescence through gibberellin pathway

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Plant Diversity 2021年第4期43卷 331-340页

作者： Haiyan Zhang Liping Zhang Songguo Wu Yanli Chen diqiu yu Ligang Chen CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunmingYunnan650223China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan666303China CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesNo.132Lanhei RoadKunmingYunnan650201China University of Chinese Academy of Sciences Beijing100049China State Key Laboratory for Conservation and Utilization of Bio-Resources in yunnan Yunnan UniversityYunnan650091China

WRKY transcription factors play essential roles during leaf ***,the mechanisms by which they regulate this process remains largely ***,we identified the transcription factor WRKY75 as a positive regulator during leaf *** of WRKY75 caused a delay in agetriggered leaf senescence,whereas overexpression of WRKY75 markedly accelerated this *** of senescence-associated genes(SAGs)was suppressed in WRKY75 mutants but increased in WRKY75-overexpressing *** analysis demonstrated that WRKY75 directly associates with the promoters of SAG12 and SAG29,to activate their ***,GAI and RGL1,two DELLA proteins,can suppress the WRKY75-mediated activation,thereby attenuating SAG expression during leaf *** analyses showed that GAI gain-of-function or RGL1 overexpression can partially rescue the accelerated senescence phenotype caused by WRKY75 ***,WRKY75 can positively regulate WRKY45 expression during leaf *** data thus imply that WRKY75 may positively modulate age-triggered leaf senescence through the gibberellin-mediated signaling pathway。

关键词： WRKY75 Leaf senescence GA DELLAs