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Lived Experience with Cirrhosis of Liver Patient at a University Hospital: A Qualitative Study

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Open Journal of Gastroenterology 2020年第10期10卷 266-280页

作者： Bushra E. Jannat Mohammad Nurul Anowar Shariful Islam dipali rani mallick Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh Faculty of Adult and Elderly Health Nursing National Institute of Advanced Nursing Education and Research (NIANER) Dhaka Bangladesh Faculty of Women’s Health and Midwifery Nursing National Institute of Advanced Nursing Education and Research (NIANER) Dhaka Bangladesh

Introduction: Patients’ physical, psychosocial, economical, spiritual and daily activities as well as quality of life are mostly affected by the cirrhosis of liver. It is an advanced stage of liver fibrosis that mostly caused by hepatitis B and hepatitis C, and alcoholism. Objective: This study is intended to explore the lived experience of the patient’s with cirrhosis of liver patients. Methodology: A Phenomenological study design was used as a research methodology to elicit in-depth understanding of lived experience of the hospitalized adult cirrhosis of liver patients at Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh. Twenty patients with cirrhosis of liver were purposively interviewed using guidelines until data were saturated. Recorded voice was checked by participants to establish trustworthiness. Interviews were recorded, transcribed verbatim and translated. Content analysis was used to explore the structure of meaning of the lived experienced cirrhosis of liver patients. Results: The average age of

关键词： Cirrhosis of Liver Lived Experience Liver Cirrhosis Patient

Insight into 5-aminolevulinic acid-induced modulation of cellular antioxidant metabolism to confer salinity and drought tolerance in maize

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BIOCELL 2020年第4期44卷 713-730页

作者： MD.ROBYUL ISLAM TAHIA NAZNIN dipali rani GUPTA MD.ASHRAFUL HAQUE MIRZA HASANUZZAMAN MD.MOTIAR ROHMAN Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Genetics and Plant Breeding Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Agronomy Faculty of AgricultureSher-e-Bangla Agricultural UniversitySher-e-Bangla NagarDhaka1207Bangladesh Molecular Breeding Laboratory Plant Breeding DivisionBangladesh Agricultural Research InstituteGazipur1701Bangladesh

The current study investigated the comparative oxidative damage in twomaize seedlings induced by saline,drought,and combined stress and the ameliorative role of two different doses(20 and 80μM)of 5-aminolevulinic acid(ALA)against the above-mentioned *** grown 10-day-old maize(Zea mays,*** Hybrid Maize-7(BHM-7)and BARI Hybrid Maize-9(BHM-9))seedlings were exposed to 12 dS/m of saline solution,200 mM mannitol-induced drought stress alone and their combined stress for 7 *** revealed that individual stresses retard the plant growth to some degrees,however,their combined stress has more detrimental effects,which might be correlated with lipid peroxidation(MDA)-induced oxidative stress in seedlings,enhanced Na+/K+ratio,and augmented generation of superoxide(O_(2)•^(−))and hydrogen peroxide(H_(2)O_(2)).In contrast,exogenous ALA supplementation at 20μM concentration markedly recovered from chlorosis and growth inhibition,substantially scavenged reactive oxygen species(ROS)and MDA by preserving ionhomeostasis and relaxing oxidative stress,also,by boosting catalase(CAT)and glutathione S-transferase(GST),and exclusively via depressing the activity of lipoxygenase(LOX)antioxidant *** the contrary,80μM ALA made things worse,nevertheless,higher activities shown by other antioxidant enzymes,like,superoxide dismutase(SOD),ascorbate peroxidase(APX),peroxidase(POD),and glutathione peroxidase(GPX),which were related to lessen the oxidative damage by highly produced O_(2)•^(−)and H_(2)O_(2) under combined ***-denaturing gel electrophoresis was done for further ***,ALA importantly increased the photosynthetic pigment contents in both genotypes irrespective of ***,GST might have assisted the plants to escape from the herbicidal effect by ***,in the combined stress condition,high ALA concentration may have some positive role to *** findings also showed that BHM-9 performed better than BHM

关键词： Maize Combined stress Salinity Drought 5-aminolevulinic acid Oxidative stress

Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology

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Engineering 2021年第9期7卷 1326-1335页

作者： Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi dipali rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology The Ohio State UniversityColumbusOH 43210USA Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityDhaka 1706Bangladesh Centro de Ciências Agrárias Universidade Federal de São CarlosArarasSP CEP 13600-000Brazil Foreign Disease-Weed Science Research Unit(FDWSRU) United States Department of Agriculture-Agricultural Research Service(USDA-ARS)Ft.DetrickMD 21702USA

Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and *** MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is *** develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)*** polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and *** test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR *** this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 *** activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)*** then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat *** novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.

关键词： Wheat blast Magnaporthe oryzae Triticum Cas12a Nucleic acid rapid lateral flow immunoassay Field detection

利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型

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Engineering 2021年第9期7卷 279-299页

作者：康厚祥彭烨 Kangyu Hua 邓雨飞 Maria Bellizzi dipali rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson 周益林周雪平 Md Tofazzal Islam 王国梁 State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Department of Plant Pathology The Ohio State University Institute of Biotechnology and Genetic Engineering (IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural University Centro de Ciências Agrárias Universidade Federal de S?o Carlos Foreign Disease-Weed Science Research Unit (FDWSRU) United States Department of Agriculture-Agricultural Research Service (USDA-ARS)

麦瘟病由稻瘟菌小麦致病型（MoT）引起，是一种存在于南美洲和孟加拉国的毁灭性病害。由于MoT通常不会在抽穗期之前使小麦显现染病症状，但在抽穗期其侵染会一直加剧，因此仅根据肉眼观察来使用杀菌剂是无效的。为了开发一种在苗期和营... 详细信息

麦瘟病由稻瘟菌小麦致病型（MoT）引起，是一种存在于南美洲和孟加拉国的毁灭性病害。由于MoT通常不会在抽穗期之前使小麦显现染病症状，但在抽穗期其侵染会一直加剧，因此仅根据肉眼观察来使用杀菌剂是无效的。为了开发一种在苗期和营养期准确、灵敏检测MoT以控制病害蔓延的方法，我们对来自巴西的两个MoT分离株的基因组进行了测序，并确定了两个DNA片段：MoT-6098和MoT-6099，它们存在于麦瘟病菌（MoT）基因组中，但不存在于感染稻瘟病菌（MoO）的水稻基因组中。利用聚合酶链反应（PCR），我们在来自南美洲和孟加拉国的53株MoT和MoO分离株中证实了这两对标记引物的特异性。为了测试这两对标记引物的有效性，我们首先建立了一种环介导等温扩增（LAMP）方法。在等温条件下，这种方法在不使用常规PCR仪器的情况下也能检测MoT。随后，我们使用Cas12a蛋白及导向RNA(g RNAs)靶向MoT-6098和MoT-6099序列，两个靶标被识别后，均能激活Cas12a非特异切割单链脱氧核糖核酸酶（ss DNAase）的活性，我们将依赖靶向Cas12a激活ss DNase活性，同时将重组酶聚合酶扩增（RPA）和核酸侧流免疫分析法（NALFIA）相结合，开发出一种准确、灵敏且经济高效地检测受感染小麦植株中MoT特异性DNA序列的方法。这种新技术可应用于田间麦瘟病和其他重要植物病害的快速检测。

关键词：麦瘟病稻瘟菌小麦致病型 Cas12a 核酸侧流免疫分析田间检测