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Insight into 5-aminolevulinic acid-induced modulation of cellular antioxidant metabolism to confer salinity and drought tolerance in maize

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BIOCELL 2020年第4期44卷 713-730页

作者： MD.ROBYUL ISLAM TAHIA NAZNIN dipali rani gupta MD.ASHRAFUL HAQUE MIRZA HASANUZZAMAN MD.MOTIAR ROHMAN Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Genetics and Plant Breeding Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Agronomy Faculty of AgricultureSher-e-Bangla Agricultural UniversitySher-e-Bangla NagarDhaka1207Bangladesh Molecular Breeding Laboratory Plant Breeding DivisionBangladesh Agricultural Research InstituteGazipur1701Bangladesh

The current study investigated the comparative oxidative damage in twomaize seedlings induced by saline,drought,and combined stress and the ameliorative role of two different doses(20 and 80μM)of 5-aminolevulinic acid(ALA)against the above-mentioned *** grown 10-day-old maize(Zea mays,*** Hybrid Maize-7(BHM-7)and BARI Hybrid Maize-9(BHM-9))seedlings were exposed to 12 dS/m of saline solution,200 mM mannitol-induced drought stress alone and their combined stress for 7 *** revealed that individual stresses retard the plant growth to some degrees,however,their combined stress has more detrimental effects,which might be correlated with lipid peroxidation(MDA)-induced oxidative stress in seedlings,enhanced Na+/K+ratio,and augmented generation of superoxide(O_(2)•^(−))and hydrogen peroxide(H_(2)O_(2)).In contrast,exogenous ALA supplementation at 20μM concentration markedly recovered from chlorosis and growth inhibition,substantially scavenged reactive oxygen species(ROS)and MDA by preserving ionhomeostasis and relaxing oxidative stress,also,by boosting catalase(CAT)and glutathione S-transferase(GST),and exclusively via depressing the activity of lipoxygenase(LOX)antioxidant *** the contrary,80μM ALA made things worse,nevertheless,higher activities shown by other antioxidant enzymes,like,superoxide dismutase(SOD),ascorbate peroxidase(APX),peroxidase(POD),and glutathione peroxidase(GPX),which were related to lessen the oxidative damage by highly produced O_(2)•^(−)and H_(2)O_(2) under combined ***-denaturing gel electrophoresis was done for further ***,ALA importantly increased the photosynthetic pigment contents in both genotypes irrespective of ***,GST might have assisted the plants to escape from the herbicidal effect by ***,in the combined stress condition,high ALA concentration may have some positive role to *** findings also showed that BHM-9 performed better than BHM

关键词： Maize Combined stress Salinity Drought 5-aminolevulinic acid Oxidative stress

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Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology

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Engineering 2021年第9期7卷 1326-1335页

作者： Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi dipali rani gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology The Ohio State UniversityColumbusOH 43210USA Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityDhaka 1706Bangladesh Centro de Ciências Agrárias Universidade Federal de São CarlosArarasSP CEP 13600-000Brazil Foreign Disease-Weed Science Research Unit(FDWSRU) United States Department of Agriculture-Agricultural Research Service(USDA-ARS)Ft.DetrickMD 21702USA

Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and *** MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is *** develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)*** polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and *** test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR *** this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 *** activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)*** then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat *** novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.

关键词： Wheat blast Magnaporthe oryzae Triticum Cas12a Nucleic acid rapid lateral flow immunoassay Field detection

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An Insight Survey on Sensor Errors and Fault Detection Techniques in Smart Spaces

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Computer Modeling in Engineering & Sciences 2024年第3期138卷 2029-2059页

作者： Sheetal Sharma Kamali gupta Deepaligupta Shalli rani Gaurav Dhiman Chitkara University Institute of Engineering and Technology Chitkara UniversityPunjab140401India PG Department of Information Technology Goswami Ganesh Dutta Sanatan Dharma CollegeChandigarh160030India Department of Computer Science Government Bikram College of CommercePatialaIndia Department of Electrical and Computer Engineering Lebanese American UniversityByblosLebanon University Centre for Research and Development Department of Computer Science and EngineeringChandigarh UniversityMohali140413India Department of Computer Science and Engineering Graphic Era Deemed to be UniversityDehradun248002India Division of Research and Development Lovely Professional UniversityPunjab144001India

The widespread adoption of the Internet of Things (IoT) has transformed various sectors globally, making themmore intelligent and connected. However, this advancement comes with challenges related to the effectiveness ofIoT devices. These devices, present in offices, homes, industries, and more, need constant monitoring to ensuretheir proper functionality. The success of smart systems relies on their seamless operation and ability to handlefaults. Sensors, crucial components of these systems, gather data and contribute to their functionality. Therefore,sensor faults can compromise the system’s reliability and undermine the trustworthiness of smart *** address these concerns, various techniques and algorithms can be employed to enhance the performance ofIoT devices through effective fault detection. This paper conducted a thorough review of the existing literature andconducted a detailed *** analysis effectively links sensor errors with a prominent fault detection techniquecapable of addressing them. This study is innovative because it paves theway for future researchers to explore errorsthat have not yet been tackled by existing fault detection methods. Significant, the paper, also highlights essentialfactors for selecting and adopting fault detection techniques, as well as the characteristics of datasets and theircorresponding recommended techniques. Additionally, the paper presents amethodical overview of fault detectiontechniques employed in smart devices, including themetrics used for evaluation. Furthermore, the paper examinesthe body of academic work related to sensor faults and fault detection techniques within the domain. This reflectsthe growing inclination and scholarly attention of researchers and academicians toward strategies for fault detectionwithin the realm of the Internet of Things.

关键词： Error fault detection techniques sensor faults outliers Internet of Things

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利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型

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Engineering 2021年第9期7卷 279-299页

作者：康厚祥彭烨 Kangyu Hua 邓雨飞 Maria Bellizzi dipali rani gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson 周益林周雪平 Md Tofazzal Islam 王国梁 State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Department of Plant Pathology The Ohio State University Institute of Biotechnology and Genetic Engineering (IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural University Centro de Ciências Agrárias Universidade Federal de S?o Carlos Foreign Disease-Weed Science Research Unit (FDWSRU) United States Department of Agriculture-Agricultural Research Service (USDA-ARS)

麦瘟病由稻瘟菌小麦致病型（MoT）引起，是一种存在于南美洲和孟加拉国的毁灭性病害。由于MoT通常不会在抽穗期之前使小麦显现染病症状，但在抽穗期其侵染会一直加剧，因此仅根据肉眼观察来使用杀菌剂是无效的。为了开发一种在苗期和营... 详细信息

麦瘟病由稻瘟菌小麦致病型（MoT）引起，是一种存在于南美洲和孟加拉国的毁灭性病害。由于MoT通常不会在抽穗期之前使小麦显现染病症状，但在抽穗期其侵染会一直加剧，因此仅根据肉眼观察来使用杀菌剂是无效的。为了开发一种在苗期和营养期准确、灵敏检测MoT以控制病害蔓延的方法，我们对来自巴西的两个MoT分离株的基因组进行了测序，并确定了两个DNA片段：MoT-6098和MoT-6099，它们存在于麦瘟病菌（MoT）基因组中，但不存在于感染稻瘟病菌（MoO）的水稻基因组中。利用聚合酶链反应（PCR），我们在来自南美洲和孟加拉国的53株MoT和MoO分离株中证实了这两对标记引物的特异性。为了测试这两对标记引物的有效性，我们首先建立了一种环介导等温扩增（LAMP）方法。在等温条件下，这种方法在不使用常规PCR仪器的情况下也能检测MoT。随后，我们使用Cas12a蛋白及导向RNA(g RNAs)靶向MoT-6098和MoT-6099序列，两个靶标被识别后，均能激活Cas12a非特异切割单链脱氧核糖核酸酶（ss DNAase）的活性，我们将依赖靶向Cas12a激活ss DNase活性，同时将重组酶聚合酶扩增（RPA）和核酸侧流免疫分析法（NALFIA）相结合，开发出一种准确、灵敏且经济高效地检测受感染小麦植株中MoT特异性DNA序列的方法。这种新技术可应用于田间麦瘟病和其他重要植物病害的快速检测。

关键词：麦瘟病稻瘟菌小麦致病型 Cas12a 核酸侧流免疫分析田间检测

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Elastodynamic analysis at an interface of viscous fluid/thermoelastic micropolar honeycomb medium due to inclined load

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Applied Mathematics and Mechanics(English Edition) 2009年第3期30卷 353-364页

作者： Rajneesh Kumar Rajani rani gupta Li-qun CHEN Department of Mathematics Kurukshetra University Kurukshetra 136119 India 不详

In this paper, the effect of angle inclination at the interface of a viscous fluid and thermoelastic micropolar honeycomb solid due to inclined load is investigated. The inclined load is assumed to be a linear combination of normal load and tangential load. Laplace transform with respect to time variable and Fourier transform with respect to space variable are applied to solve the problem. Expressions of stresses, temperature distribution, and pressures in the transformed domain are obtained by introducing potential functions. The numerical inversion technique is used to obtain the solution in the physical domain. The frequency domain expressions for steady state are also obtained with appropriate change of variables. Graphic representations due to the response of different sources and changes of angle inclination are shown. Some particular cases are also discussed.

关键词： viscous fluid thermoelastic micropolar honeycomb material inclined load time harmonic steady state

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Phenotypic and molecular diversity-based prediction of heterosis in pearl millet(Pennisetum glaucum L.(R.)Br.)

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The Crop Journal 2018年第3期6卷 271-281页

作者： Shashi Kumar gupta Thirunavukkarasu Nepolean Chinna Ghouse Shaikh Kedarnath Rai Charles Thomas Hash Roma rani Das Abhishek Rathore International Crops Research Institute for the Semi-Arid Tropics(ICRISAT) Patancheru 502 324India Indian Agricultural Research Institute(IARI) New Delhi 110012India International Crops Research Institute for the Semi-Arid Tropics(ICRISAT) Niamey BP 12404Niger

Genetic distances between hybrid parents based on phenotypic traits and molecular markers were investigated to assess their relationship with heterosis for grain and stover yield and other traits in pearl millet(Pennisetum glaucum [L.] R. Br.). Fifty-one hybrids developed using 101 hybrid parents(B and R lines) and showing a wide range of genetic distance between their parents based on eight phenotypic traits and 28–38 SSRs were evaluated in two sets for two seasons. The correlation between Euclidean distance(phenotypic distance, ED) and simple matching distance(molecular distance, SM) for parents of both sets was low but positive and significant(r = 0.2, P < 0.001).The correlation of ED in parents with better-parent heterosis for grain yield was similar in both sets(r =0.38, P < 0.05). SM was not correlated with heterosis for grain yield in either set of *** results showed that phenotypic distance could be a better predictor of heterosis than molecular distance. The correlation between phenotypic distance and heterosis was not strong enough to permit the use of phenotypic diversity among parents as a major selection criterion for selection of parental lines displaying high levels of heterosis for grain and stover yield in pearl millet.

关键词： Hybrid parents Agronomic traits Euclidean distance Molecular markers Simple matching distance

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Disease detection,severity prediction,and crop loss estimation in MaizeCrop using deep learning

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Artificial Intelligence in Agriculture 2022年第1期6卷 276-291页

作者： Nidhi Kundu Geeta rani Vijaypal Singh Dhaka Kalpit gupta Siddaiah Chandra Nayaka Eugenio Vocaturo Ester Zumpano Department of Computer and Communication Engineering Manipal University JaipurJaipurIndia ICAR DOS in Biotechnology University of Mysore ManasagangotriMysore 570005India DIMES(Department of Computer Engineering ModelingElectronics and Systems)University of CalabriaItaly Nanotec Italian National Research Council87036 RendeCSItaly

The increasing gap between the demand and productivity of maize crop is a point of concern for the food industry,and ***'susceptibility to diseases such as Turcicum Leaf Blight,and Rust is a major cause for reducing its *** detection,and classification of these diseases,calculation of disease severity,and crop loss estimation is a time-consuming ***,it requires expertise in disease ***,there is a need to find an alternative for automatic disease detection,severity prediction,and crop loss *** promising results of machine learning,and deep learning algorithms in pattern recognition,object detection,and data analysis motivate researchers to employ these techniques for disease detection,classification,and crop loss estimation in maize *** research works available in literature,have proven their potential in automatic disease detection using machine learning,and deep learning ***,there is a lack none of these works a reliable and real-life labelled dataset for training these ***,none of the existing works focus on severity prediction,and crop loss *** authors in this manuscript collect the real-life dataset labelled by plant *** propose a deep learning-based framework for pre-processing of dataset,automatic disease detection,severity prediction,and crop loss *** uses the K-Means clustering algorithm for extracting the region of ***,they employ the customized deep learning model‘MaizeNet’for disease detection,severity prediction,and crop loss *** model reports the highest accuracy of 98.50%.Also,the authors perform the feature visualization using the ***,the proposed model is integrated with a web application to provide a userfriendly *** efficacy of the model in extracting the relevant features,a smaller number of parameters,low training time,high accuracy favors its importance as an assisting tool for plant pathology experts.T

关键词： Disease detection Crop loss Severity Deep learning Maize

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Mothers Experience about Complementary Feeding Practice in Bangladesh

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Open Journal of Pediatrics 2020年第3期10卷 423-437页

作者： Kuntala Gain Shanzida Khatun Md. Sazzad Hossain dipali rani Mallick Shaheed Tajuddin Ahmad Nursing College Gazipur Dhaka Bangladesh National Institute of Advanced Nursing Education and Research (NIANER) Dhaka Bangladesh

Background: Complementary feeding is a significant milestone that has nutritional, developmental, and health implications. Worldwide, 33% of the deaths are linked to malnutrition. Objective: To describe the mothers’ experience about complementary feeding practice with child aged less than two years in Bangladesh. Method: A descriptive qualitative study was conducted from July 2018 to June 2019. This study was approved by the Institutional Review Board (IRB) NIANER. 20 mothers of children of 6 - 24 months of age were recruited purposively who live in Sattalla slum. Descriptive statistics were used to analyze the mothers’ demographic characteristics and thematic analysis was used to describe mothers’ experience about complementary feeding practice. Results: The study showed that the average age of the mothers was 25 (SD = 4.75) years. Only 5% of the mothers have higher education. Through the findings, two main themes have been identified. Moreover, first main theme includes three subthemes. Fifty percent of the mothers stated that appropriate time to start complementary food for their babies is from 6 months of age. Mothers feed various types of complementary foods. Most of the mothers have knowledge about complementary feeding but they do not practice appropriately. Almost all of the mothers (95%) stated that their babies do not like to eat anything, and 45% of mothers feed only breast milk. Conclusion: Findings of the study will provide information for the nurses resulted in increasing the rate of complementary feeding practice am

关键词： Experience Complementary Feeding Practice Mothers Children

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Lived Experience with Cirrhosis of Liver Patient at a University Hospital: A Qualitative Study

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Open Journal of Gastroenterology 2020年第10期10卷 266-280页

作者： Bushra E. Jannat Mohammad Nurul Anowar Shariful Islam dipali rani Mallick Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh Faculty of Adult and Elderly Health Nursing National Institute of Advanced Nursing Education and Research (NIANER) Dhaka Bangladesh Faculty of Women’s Health and Midwifery Nursing National Institute of Advanced Nursing Education and Research (NIANER) Dhaka Bangladesh

Introduction: Patients’ physical, psychosocial, economical, spiritual and daily activities as well as quality of life are mostly affected by the cirrhosis of liver. It is an advanced stage of liver fibrosis that mostly caused by hepatitis B and hepatitis C, and alcoholism. Objective: This study is intended to explore the lived experience of the patient’s with cirrhosis of liver patients. Methodology: A Phenomenological study design was used as a research methodology to elicit in-depth understanding of lived experience of the hospitalized adult cirrhosis of liver patients at Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh. Twenty patients with cirrhosis of liver were purposively interviewed using guidelines until data were saturated. Recorded voice was checked by participants to establish trustworthiness. Interviews were recorded, transcribed verbatim and translated. Content analysis was used to explore the structure of meaning of the lived experienced cirrhosis of liver patients. Results: The average age of

关键词： Cirrhosis of Liver Lived Experience Liver Cirrhosis Patient

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Posterior Capsular Rent: Risk Factors, Diagnosis and Management

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Surgical Science 2014年第5期5卷 224-226页

作者： Nimmi rani Prashant gupta Aravind Eye Hospital Madurai India Cataract Services at Aravind Eye Hospital Madurai India

In spite of a steady improvement in the safety of cataract surgery since the inception of phacoemulsification, diagnosing and managing posterior capsular rent still remains a challenge. Identification of the pre-existing risk factors is of utmost importance so that precautionary modifications are done in the surgery to prevent this complication. Once it happens, timely realization and appropriate management can have excellent outcomes.

关键词： Posterior Capsular Rent Vitreous Loss Traumatic Cataract Small Pupil Pseudoexfoliation Vitrectomy Triamcinolone Acetonide

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