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Structural variations of a new fertility restorer gene,Rf20,underlie the restoration of wild abortive-type cytoplasmic male sterility in rice

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Molecular Plant 2024年第8期17卷 1272-1288页

作者： Shufeng Song Yixing Li Mudan Qiu Na Xu Bin Li Longhui Zhang Lei Li Weijun Chen Jinglei Li Tiankang Wang Yingxin Qiu Mengmeng Gong Dong Yu Hao Dong Siqi Xia Yi Pan dingyang yuan Li Li State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterHunan Academy of Agricultural SciencesChangsha 410125China College of Agronomy Hunan Agricultural UniversityChangsha 410128China College of Plant Protection Hunan Agricultural UniversityChangsha 410128China College of Tropical Crops Hainan UniversityHaikou 570228China Longping Branch College of BiologyHunan UniversityChangsha 410125China

The discovery of a wild abortive-type(WA)cytoplasmic male sterile(CMS)line and breeding its restorer line have led to the commercialization of three-line hybrid rice,contributing considerably to global food ***,the molecular mechanisms underlyingfertility abortion and the restoration of CMs-WA lines remain largely *** this study,we cloned a restorer gene,Rf2o,following a genome-wide association study analysis of the core parent lines of three-line hybrid *** found that Rf20 was present in all core parental lines,but different haplotypes and structural variants of its gene resulted in differences in Rf2o expression levels between sterileand restored ***20 could restorepollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMs lines under high *** addition,we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with *** interaction enhances Cox11's function as a scavenger of reactive oxygen species,which in turn restores ***,ourstudysuggestsanewactionmodeforpentatricopeptide repeatproteins inthe fertilityrestorationofCMs lines,providing an essential theoretical basisforbreedingrobustrestorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.

关键词： COX11 cytoplasmic male sterility rf20 WA352 wild abortion

The OsWRKY72-OsAAT30/OsGSTU26 module mediates reactive oxygen species scavenging to drive heterosis for salt tolerance in hybrid rice

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Journal of Integrative Plant Biology 2024年第4期66卷 709-730页

作者： Citao Liu Bigang Mao Yanxia Zhang Lei tian Biao Ma Zhuo Chen Zhongwei Wei Aifu Li Ye Shao Gongye Cheng Lingling Li Wenyu Li Di Zhang Xiaoping Ding Jiangxiang Peng Yulin Peng Jiwai He Nenghui Ye dingyang yuan Chengcai Chu Meijuan Duan Hunan Provincial Key Laboratory of Stress Biology College of AgricultureHunan Agricultural UniversityChangsha 410128China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125China College of Agriculture South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Wheat and Maize Crop Science Center for Crop Genome EngineeringCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMI 48109 USA Biobin Data Science Co. Ltd.Changsha 410221China

Hybrid rice(Oryza sativa)generally outperforms its inbred parents in yield and stress tolerance,a phenomenon termed heterosis,but the underlying mechanism is not completely ***,we combined transcriptome,proteome,physiological,and heterosis analyses to examine the salt response of super hybrid rice Chaoyou1000(CY1000).In addition to surpassing the mean values for its two parents(mid-parent heterosis),CY1000 exhibited a higher reactive oxygen species scavenging ability than both its parents(over-parent heterosis or heterobeltiosis).Nonadditive expression and allele-specific gene expression assays showed that the glutathione S-transferase gene OsGSTU26 and the amino acid transporter gene OsAAT30 may have major roles in heterosis for salt tolerance,acting in an overdominant fashion in ***,we identified OsWRKY72 as a common transcription factor that binds and regulates OsGSTU26 and *** salt-sensitive phenotypes were associated with the OsWRKY72^(paternal)genotype or the OsAAT30^(maternal)genotype in core rice germplasm ***72^(paternal)specifically repressed the expression of OsGSTU26 under salt stress,leading to salinity sensitivity,while OsWRKY72^(maternal)specifically repressed OsAAT30,resulting in salinity *** results suggest that the OsWRKY72-OsAAT30/OsGSTU26 module may play an important role in heterosis for salt tolerance in an overdominant fashion in CY1000 hybrid rice,providing valuable clues to elucidate the mechanism of heterosis for salinity tolerance in hybrid rice.

关键词： allele-specific gene expression(ASE) heterosis hybrid rice nonadditive effect(NAE) salt tolerance transcriptome and proteome

一种利用IIS型限制性内切酶进行无缝堆垒的酶促组装方法

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分子植物育种 2024年第7期22卷 2407-2412页

作者：唐焱锋周天顺刘玲余东董皓段美娟袁定阳湖南大学研究生院隆平分院长沙410125 湖南杂交水稻研究中心杂交水稻全国重点实验室长沙410125 湖南省农业科学院长沙410125 湖南农业大学农学院长沙410125

酶切-酶连是分子组装常用的传统重组方法,但其应用受到组装效率低、限制性酶切位点少、易产生突变碱基及无法实现无缝克隆等因素制约。为克服以上限制因素,我们提出一种利用IIS型限制性内切酶进行无缝堆垒的酶促组装方法。该方法是一种... 详细信息

酶切-酶连是分子组装常用的传统重组方法,但其应用受到组装效率低、限制性酶切位点少、易产生突变碱基及无法实现无缝克隆等因素制约。为克服以上限制因素,我们提出一种利用IIS型限制性内切酶进行无缝堆垒的酶促组装方法。该方法是一种利用IIS型限制性内切酶识别位点位于切割位点之外的特性,在线性化质粒载体和与目的片段同源重组过程中不会破坏识别位点,通过多轮酶促组装实现目的序列的无缝克隆的二元酶促组装方法。我们通过该方法对EAT1基因CDs序列组装验证了该方法的可行性与实用性。

关键词：无缝堆垒酶促组装 IIS型限制性内切酶

Salt tolerance in rice:Physiological responses and molecular mechanisms

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The Crop Journal 2022年第1期10卷 13-25页

作者： Citao Liu Bigang Mao dingyang yuan Chengcai Chu Meijuan Duan College of Agriculture Hunan Agricultural UniversityChangsha 410128HunanChina State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125HunanChina State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China

Crop yield loss due to soil salinization is an increasing threat to agriculture *** stress drastically affects the growth,development,and grain productivity of rice(Oryza sativa L.),and the improvement of rice tolerance to salt stress is a desirable approach for meeting increasing food *** main contributors to salt toxicity at a global scale are Na^(+)and Cl^(-)ions,which affect up to 50%of irrigated *** responses to salt stress occur at the organismic,cellular,and molecular levels and are pleiotropic,involving(1)maintenance of ionic homeostasis,(2)osmotic adjustment,(3)ROS scavenging,and(4)nutritional *** this review,we discuss recent research progress on these four aspects of plant physiological response,with particular attention to hormonal and gene expression regulation and salt tolerance signaling pathways in *** information summarized here will be useful for accelerating the breeding of salt-tolerant rice.

关键词： Salt stress Rice(Oryza sativa L.) Salt tolerance genes Physiological response Salt signal transduction

水稻矮秆迟抽穗突变体d534的性状及其对赤霉素的敏感性分析

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中国农业科技导报 2024年第3期26卷 7-14页

作者：曾建光刘桃李孙林娟袁定阳黄钰博金晨钟谭炎宁湖南人文科技学院农业与生物技术学院湖南娄底417000 国家耐盐碱水稻技术创新中心海南三亚572000 海南大学热带作物学院海口570100

水稻半矮秆突变体在株型改良和高产育种中具有重要价值。前期通过辐射诱变籼稻品种五山丝苗(R534)获得了半矮秆且抽穗期延迟突变体d534,分析了d534的表型、遗传模式和GA3敏感性。大田性状分析发现,和野生型R534相比,d534的抽穗期延迟了7... 详细信息

水稻半矮秆突变体在株型改良和高产育种中具有重要价值。前期通过辐射诱变籼稻品种五山丝苗(R534)获得了半矮秆且抽穗期延迟突变体d534,分析了d534的表型、遗传模式和GA3敏感性。大田性状分析发现,和野生型R534相比,d534的抽穗期延迟了7 d,株高下降了29.22 cm,降幅为26.86%。d534的穗及倒1至倒5节都明显缩短,长度分别为野生型的71.27%、68.75%、70.18%、85.17%、77.86%和71.18%,说明d534的目的基因调控穗及茎节的伸长。遗传分析发现,d534的矮化和迟抽穗性状受同一核基因控制,呈隐性遗传。内源GA3含量测定发现,一叶一心期d534茎中GA3含量为0.17 ng·g^(-1),比R534降低了60.47%,表明d534的矮化与内源GA3合成不足有关。用25和50 mg·L^(-1)外源GA3处理一叶一心期幼苗5 d,d534的株高比未处理组(0 mg·L^(-1)GA3)增加了71.76%和74.98%,且超过了未处理组R534的株高,说明外源GA3能回补d534的株高表型;qRT-PCR分析发现,与R534相比,d534茎和叶中OsGA20ox2和OsGA3ox2基因表达水平均显著下调,表明d534内源GA3合成不足可能与OsGA20ox2和OsGA3ox2基因表达水平下调有关。明确了d534是茎节缩短且内源GA3积累不足的矮化突变体,为解析d534半矮秆性状形成的生物学机理奠定了基础。

关键词：水稻 d534突变体半矮秆迟抽穗赤霉素

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多穗型杂交稻在不同肥力下生长与产量表现研究

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杂交水稻 2024年第1期39卷 102-111页

作者：张稳彭锐袁定阳孙佳丽唐自标刘梓琦王学华彭既明湖南农业大学湖南长沙410125 湖南杂交水稻研究中心湖南长沙410125 杂交水稻全国重点实验室湖南长沙410125 湖南神州星锐种业科技有限公司湖南长沙410125

通过对多穗型(明两优143)、大穗型(湘两优900)和中大穗型(丰两优4号)3个杂交稻品种在4种肥力水平(纯N用量0、150、225、300 kg/hm^(2))下的农艺性状、碳氮代谢关键酶活性和产量的表现进行研究,探索肥力水平对多穗型杂交稻的生长和产量... 详细信息

通过对多穗型(明两优143)、大穗型(湘两优900)和中大穗型(丰两优4号)3个杂交稻品种在4种肥力水平(纯N用量0、150、225、300 kg/hm^(2))下的农艺性状、碳氮代谢关键酶活性和产量的表现进行研究,探索肥力水平对多穗型杂交稻的生长和产量的影响。结果表明,随着施肥水平的提高,3个品种的产量均同步提高,且肥力水平的提高对有效穗数、最高茎蘖数、株高、干物质量以及碳氮代谢关键酶活性均有促进作用,对结实率和成穗率有抑制作用,但对千粒重和每穗总粒数的影响不明显。多穗型品种明两优143在高肥水平(纯N用量300 kg/hm^(2))下达到3个品种中的最高产量,为9658.33 kg/hm^(2),其高产的主要原因是单位面积有效穗数的显著增加。多穗型品种明两优143齐穗期和乳熟期的地上部干物质量明显高于其他2个品种;其蔗糖磷酸合成酶(SPS)活性在花后第10天达最高值,比其他2个品种提早5或10 d,其硝酸还原酶(NR)活性在开花后第15天达到峰值,较其他2个品种延后5 d,这样使它能在灌浆期维持更长时间的较高酶活性水平,增强了光合能力,缩短了灌浆时间,从而保障其稳产高产。

关键词：多穗型杂交稻肥力水平农艺性状干物质量酶活性产量

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矮秆优质水稻光温敏核不育系盛1618S的选育与利用

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杂交水稻 2024年第4期39卷 42-46页

作者：张志刚杨益善艾治勇张志华罗孝和袁定阳唐文帮许靖波三亚市国家耐盐碱水稻技术创新中心海南三亚572000 湖南杂交水稻研究中心湖南长沙410125

盛1618S是湖南杂交水稻研究中心和国家耐盐碱水稻技术创新中心利用Y58S与P88S杂交,后代经过系谱法选择育成的水稻光温敏核不育系,2019年通过安徽省农作物品种审定委员会鉴定。该不育系植株矮健,分蘖和抗倒伏能力强,配合力强,米质优良,... 详细信息

盛1618S是湖南杂交水稻研究中心和国家耐盐碱水稻技术创新中心利用Y58S与P88S杂交,后代经过系谱法选择育成的水稻光温敏核不育系,2019年通过安徽省农作物品种审定委员会鉴定。该不育系植株矮健,分蘖和抗倒伏能力强,配合力强,米质优良,繁殖制种产量高。利用盛1618S配组的杂交组合盛两优358于2022年通过湖南省农作物品种审定。针对盛1618S矮秆基因和配组亲本选择问题进行了讨论。

关键词：杂交水稻光温敏核不育系盛1618S 矮秆优质选育

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Lyase Activities of Heterologous CpcS and CpcT for Phycocyanin Holo-β-subunit from Arthrospira platensis in Escherichia coli

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Journal of Ocean University of China 2014年第3期13卷 497-502页

作者： YI Junjie XU Di ZANG Xiaonan yuan dingyang ZHAO Bingran TANG Li TAN Yanning ZHANG Xuecheng Key Laboratory of Marine Genetics and Breeding (Ocean University of China) Ministry of Education State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center

Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli.

关键词： Arthrospira CpcB phycocyanobilin lyase CpcS CpcT

OsNramp5基因变异影响水稻重要农艺性状的研究进展

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中国水稻科学 2022年第6期36卷 562-571页

作者：李小秀吕启明袁定阳湖南大学研究生院隆平分院长沙410125 湖南杂交水稻研究中心/杂交水稻国家重点实验室长沙410125

筛选和培育镉(Cd)低积累水稻品种是解决稻米镉污染问题最经济、有效的办法。现有研究表明OsNramp5是介导水稻Cd吸收最重要的基因,其功能缺失后,水稻籽粒Cd含量极显著下降,但同时会影响水稻必需元素锰(Mn)的吸收,而在前人关于OsNramp5变... 详细信息

筛选和培育镉(Cd)低积累水稻品种是解决稻米镉污染问题最经济、有效的办法。现有研究表明OsNramp5是介导水稻Cd吸收最重要的基因,其功能缺失后,水稻籽粒Cd含量极显著下降,但同时会影响水稻必需元素锰(Mn)的吸收,而在前人关于OsNramp5变异影响水稻生长发育的研究中,结论并不一致。系统了解OsNramp5基因变异对水稻重要农艺性状的影响有助于推动低Cd优质水稻新品种的培育。本文重点对OsNramp5基因变异对水稻中金属离子的含量,水稻的生长发育、产量性状及米质的影响进行了综述,以期为利用OsNramp5基因突变选育低Cd积累水稻品种提供参考。

关键词：水稻 OsNramp5 镉锰农艺性状