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A nuclear-encoded mitochondrial gene AtCIB22 is essential for plant development in Arabidopsis

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Journal of Genetics and Genomics 2010年第10期37卷 667-683页

作者： Lihua Han Genji Qin dingming kang Zhangliang Chen Hongya Gu Li-Jia Qu College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China National Laboratory of Protein Engineering and Plant Genetic Engineering Peking University Beijing 100871 China The National Plant Gene Research Center (Beijing) Beijing 100101 China

Complex I （the NADH：ubiquinone oxidoreductase） of the mitochondrial respiratory chain is a complicated, multi-subunit, membrane- bound assembly and contains more than 40 different proteins in higher plants. In this paper, we characterize the Arabidopsis homologue （designated as AtCIB22） of the B22 subunit of eukaryotic mitochondriai Complex I. AtCIB22 is a single-copy gene and is highly con- served throughout eukaryotes. AtCIB22 protein is located in mitochondria and the AtC1B22 gene is widely expressed in different tissues. Mutant Arabidopsis plants with a disrupted AtC1B22 gene display pleiotropic phenotypes including shorter roots, smaller plants and de- layed flowering. Stress analysis indicates that the AtC1B22 mutants＇ seed germination and early seedling growth are severely inhibited by sucrose deprivation stress but more tolerant to ethanol stress. Molecular analysis reveals that in moderate knockdown AtCIB22 mutants, genes including cell redox proteins and stress related proteins are significantly up-regulated, and that in severe knockdown AtCIB22 mu- tants, the alternative respiratory pathways including NDA1, NDB2, AOXla and AtPUMP1 are remarkably elevated. These data demon- strate that AtCIB22 is essential for plant development and mitochondrial electron transport chains in Arabidopsis. Our findings also en- hance our understanding about the physiological role of Complex I in plants.

关键词： mitochondria Complex I B22 subunit ethanol treatment alternative oxidase uncoupling protein

The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis

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Journal of Integrative Plant Biology 2013年第1期55卷 64-74页

作者： Yanbing Wang Yingnan Hou Hongya Gu dingming kang Zhang-Liang Chen Jingjing Liu Li-Jia Qu State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University College of Agronomy and Biotechnology China Agricultural University The National Plant Gene Research Center

Anaphase-promoting complex/cyclosome （APC/C）, a multisubunit E3 ligase, plays a critical role in cell cycle control, but the functional characterization of each subunit has not yet been completed. To investigate the function of APC1 in Arabidopsis, we analyzed four mutant alleles of APC1, and found that mutation in APC1 resulted in significantly reduced plant fertility, accumulation of cyclin B, and disrupted auxin distribution in embryos. The three mutant alleles apcl-1, apcl-2 and apcl-3 shared variable defects in female gametogenesis including degradation, abnormal nuclear number, and disrupted polarity of nuclei in the embryo sac as well as in embryogenesis, in which embryos were arrested at multiple stages. All of these defects are similar to those previously identified in apc4. The mutant apcl-4, in which the T-DNA was inserted after the transmembrane domain at the C-terminus, showed much more severe phenotypes; that is, most of the ovules were arrested at the one-nucleate female gametophyte stage （stage FG1）. In the apcl apc4 double mutants, the fertility was further reduced by one-third in apcl-ll＋ apc4-1/＋, and in some cases no ovules even survived in siliques of apcl-4/＋ apc4-1/＋. Our data thus suggest that APC1, an essential component of APC/C, plays a synergistic role with APC4 both in female gametogenesis and in embryogenesis.

关键词： Arabidopsis anaphase-promoting complex/cyclosome (APC/C) APC1 APC4 female gametogenesis embryogenesis synergistic role

Identification of traits and genes associated with lodging resistance in maize

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The Crop Journal 2021年第6期9卷 1408-1417页

作者： Yu Guo Yumei Hu Huan Chen Pengshuai Yan Qingguo Du Yafei Wang Hongqiu Wang Zhonghua Wang dingming kang Wen-Xue Li Key Laboratory of Crop Heterosis and Utilization Ministry of EducationCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Engineering Laboratory for Crop Molecular Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Lodging is a major problem limiting maize yield worldwide. However, the mechanisms of lodging resistance remain incompletely understood for maize. Here, we evaluated 443 maize accessions for lodging resistance in the field. Five lodging-resistant accessions and five lodging-sensitive accessions were selected for further research. The leaf number, plant height, stem diameter, and rind penetrometer resistance were similar between lodging-resistant and-sensitive inbred lines. The average thickness of sclerenchymatous hypodermis layer was thicker and the vascular area was larger in the lodging-resistant lines compared with lodging-sensitive lines. Although total lignin content in stem tissue did not significantly differ between lodging-resistant and-sensitive lines, phloroglucinol staining revealed that the lignin content of the cell wall in the stem cortex and in the stem vascular tissue near the cortex was higher in the lodging-resistant lines than in the lodging-sensitive lines. Analysis of strand-specific RNA-seq transcriptome showed that a total of 793 genes were up-regulated and 713 genes were down-regulated in lodging-resistant lines relative to lodging-sensitive lines. The up-regulated genes in lodging-resistant lines were enriched in cell wall biogenesis. These results indicated that modification of cell wall biosynthesis would contribute to lodging resistance of maize.

关键词： Lodging Sclerenchyma cell Lignin Differentially expressed genes Maize

HTPdb and HTPtools:Exploiting maize haplotypetag polymorphisms for germplasm resource analyses and genomics-informed breeding

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Plant Communications 2022年第4期3卷 54-63页

作者： Yikun Zhao Hongli Tian Chunhui Li Hongmei Yi Yunlong Zhang Xiaohui Li Han Zhao Yongxue Huo Rui Wang dingming kang Yuncai Lu Zhihao Liu Ziyue Liang Liwen Xu Yang Yang Ling Zhou Tianyu Wang Jiuran Zhao Fengge Wang Maize Research Center Beijing Academy of Agriculture&Forestry Sciences(BAAFS)Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular BreedingShuguang Garden Middle Road No.9Beijing 100097China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Jilin Academy of Agricultural Sciences Maize Research InstituteGongzhuling 136100China Provincial Key Laboratory of Agrobiology Institute of Crop Germplasm and BiotechnologyJiangsu Academy of Agricultural SciencesNanjing 210014China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China College of Advanced Agriculture and Ecological Environment Heilongjiang UniversityHarbin 150080China

Along with rapid advances in high-throughput-sequencing technology,the development and application of molecular markers has been critical for the progress that has been made in crop breeding and genetic *** molecular markers should be able to rapidly genotype tens of thousands of breeding accessions with tens to hundreds of *** this study,we developed a multiplex molecular marker,the haplotype-tag polymorphism(HTP),that integrates Maize6H-60K array data from 3,587 maize inbred lines with 6,375 blocks from the recombination block *** applying strict filtering criteria,we obtained 6,163 highly polymorphic HTPs,which were evenly distributed in the ***,we developed a genome-wide HTP analysis toolkit,HTPtools,which we used to establish an HTP database(HTPdb)covering the whole genomes of 3,587 maize inbred lines commonly used in breeding.A total of 172,921 non-redundant HTP allelic variations were *** major HTPtools modules combine seven algorithms(e.g.,chain Bayes probability and the heterotic-pattern prediction algorithm)and a new plotting engine named“BCplot”that enables rapid visualization of the background information of multiple backcross *** was designed for big-data analyses such as complex pedigree reconstruction and maize heterotic-pattern *** HTP-based analytical strategy and the toolkit developed in this study are applicable for high-throughput genotyping and for genetic mapping,germplasm resource analyses,and genomics-informed breeding in maize.

关键词： haplotype tag database maize pedigree reconstruction heterotic-pattern prediction genomicsinformed breeding

Study on the Spatiotemporal Pattern Evolution and Influencing Factors of Population Aging in Henan Province

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Journal of Geodesy and Geoinformation Science 2024年第2期7卷 80-94页

作者： LIU dingming DU Jiusheng WANG Yu YANG Junping School of Surveying and Land Information Engineering Henan Polytechnic UniversityJiaozuo 454003China

This study utilized census data from Henan Province for the years 2000,2010,and 2020 to investigate the spatiotemporal evolution of population aging,defined by the proportion of the population aged 65 and *** spatial analysis techniques such as spatial autocorrelation and the standard deviation ellipse,the research mapped out the progression and distribution of aging ***,the study delved into the influencing factors of aging using an optimal parameters-based geographical *** indicate a deepening degree of population aging in Henan Province,transitioning from an adult type to an old type *** is a marked positive spatial correlation among counties,with high-value aging areas initially decreasing,then increasing,and notably spreading from the central to the central and southern regions of the *** center of gravity for population aging,specifically around Changge City and Xuchang City,exhibits a trajectory moving southeast before shifting *** detection reveals that in 2000,2010,and 2020,the elderly dependency ratio predominantly influences the aging trend,with explanatory powers of 88.4%,87.9%,and 90.9%,*** analysis indicates that the interaction between the old-child ratio and the elderly dependency ratio has a strong explanatory power for the aging patterns in Henan Province,reaching 97.3%,97.0%,and 97.4%,respectively.

关键词： population aging spatial pattern evolution spatial autocorrelation influencing factors optimal parameters-based geographical detector

Evaluation of Traffic Signs and Markings Considering Human Drivers and Automated Vehicles

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Journal of Traffic and Transportation Engineering(English Edition) 2024年

作者： Xuesong Wang Junyu Huo dingming Qin Qian Liu School of Transportation， Tongji University The Key Laboratory of Road and Traffic Engineering， Ministry of Education

Traffic signs and markings (TSMs) are necessary components of roadway infrastructure. Currently， the design of TSMs primarily caters to the needs of human drivers， without considering the requirements of automated vehicles (AVs). This study proposes a readiness evaluation framework for TSMs that assesses the effectiveness of their physical properties and roles in the driving tasks from the perspectives of both human drivers and AVs and compares the perspectives based on human drivers and AVs perceptual differences. This study chose a crash-prone roadway as the research object. For human drivers， subjective assessment is employed to evaluate the physical attributes and driving task effects of TSMs. For AVs， video recognition and simulation methods are used to assess the physical attributes and driving task effects of TSMs， respectively. The results indicate that there are differences in the perception of TSMs between human drivers and AVs. This finding emphasizes the need for future transportation infrastructure design to consider and understand the distinct requirements of autonomous vehicles and human drivers. Optimizing transportation infrastructure should ensure that they can be accurately recognized and understood by AVs. This research provides direction and guidance for the future design and optimization of TSMs that meet the safety needs of both human drivers and automated vehicles driving together.

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Study on the Spatiotemporal Pattern Evolution and Influencing Factors of Population Aging in Henan Province

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Journal of Geodesy and Geoinformation Science 2024年第2期 80-94页

作者： LIU dingming DU Jiusheng WANG Yu YANG Junping School of Surveying and Land Information Engineering Henan Polytechnic University

This study utilized census data from Henan Province for the years 2000, 2010, and 2020 to investigate the spatiotemporal evolution of population aging, defined by the proportion of the population aged 65 and *** spatial analysis techniques such as spatial autocorrelation and the standard deviation ellipse, the research mapped out the progression and distribution of aging demographics. Furthermore, the study delved into the influencing factors of aging using an optimal parameters-based geographical detector. Results indicate a deepening degree of population aging in Henan Province, transitioning from an adult type to an old type structure. There is a marked positive spatial correlation among counties, with high-value aging areas initially decreasing, then increasing, and notably spreading from the central to the central and southern regions of the province. The center of gravity for population aging, specifically around Changge City and Xuchang City, exhibits a trajectory moving southeast before shifting northwest. Factor detection reveals that in 2000, 2010, and 2020, the elderly dependency ratio predominantly influences the aging trend, with explanatory powers of 88.4%, 87.9%, and90.9%, respectively. Interaction analysis indicates that the interaction between the old-child ratio and the elderly dependency ratio has a strong explanatory power for the aging patterns in Henan Province, reaching 97.3%, 97.0%,and 97.4%, respectively.

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Identification and Analysis of a Salt Stress Related Homolog GhCIPK6 From Upland Cotton(Gossypium hirsutum L.)

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Identification and Analysis of a Salt Stress Related Homolog...

Characterization and functional analysis of maize df in a new mutant allele

第十二届全国植物基因组学大会

作者： Ying Su,Junbo Zhen,Xi Zhang,dingming kang,Jinping Hua~* College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China

Cotton is an important economic *** salinity,as well as other abiotic stresses,such as drought and extreme temperature,always damages the growth and productivity of *** upland cotton (Gossypium hirsutum L.) is a medium salt resistant crop,it will be helpful to breed the salt tolerant lines and to understand the mechanism of improving the yield potential under salt stress by isolating and transgening the salt-stress-related *** on previous study,an SSH library of cotton roots under salt stress was constructed,and several salt stress related homologs obtained and verified by RT-PCR *** of the

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Characterization and functional analysis of maize df in a ne...