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Science China(Life Sciences) 2008年第9期51卷 821-826页

作者： ding jiabo CUI ZhiZhong JIANG ShiJin LI YanPeng China Institute of Veterinary Drug Control Beijing 100081China Animal Science and Technology College Shandong Agricultural UniversityTaian 271018China

In the latest report, Chloramphenicol acetyltransferase (CAT) gene was used as a reporter to investi-gate the influence of pp38 on its upstream bi-directional promoter, and it was found that the co-expression of pp38 and pp24 can significantly enhance the transactivity of the bi-directional pro-moter between pp38 gene and 1.8-kb mRNA transcript in genome of Marek’s disease virus (MDV). In this study, enhanced green fluorescence protein (EGFP) gene was used as another reporter to further investigate the promoter activity. The transfection shows the promoter has the complete activity under the condition of co-expression of pp38 and pp24 in the same cells. Immunoprecipitation test was used to verify the structure of pp38/pp24 heteropolymer. The pp38-specific monoclonal antibody H19 was used in this test, and pp38, pp24 or both were prepared from the pcDNA-pp38, pcDNA-pp24 or pBud-pp38-pp24 transfected chicken embryonic fibroblast (CEF), respectively. Immunoprecipitation indicates that pp24 could be co-precipitated with pp38 by MabH19, implying that pp24 and pp38 were able to form a heteropolymer in the natural condition. The two separated tests clarify that pp38 and pp24 form a heteropolymer, which enhances the activity of the promoter.

关键词： Marek’s disease virus(MDV) pp38/pp24 heteropolymer bi-directional promoter

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新时代背景下的兽医微生物学发展

微生物学通报 2022年第12期49卷 4961-4963页

作者：丁家波中国农业科学院北京畜牧兽医研究所北京100193

2017年12月,《微生物学通报》首次与中国微生物学会兽医微生物学专业委员会合作推出“兽医微生物学主题刊”,集中刊登了兽医微生物学研究领域的病原分离鉴定、诊断方法、分子流行病学及兽用生物制品研发等方面的最新进展及成果。主题刊... 详细信息

2017年12月,《微生物学通报》首次与中国微生物学会兽医微生物学专业委员会合作推出“兽医微生物学主题刊”,集中刊登了兽医微生物学研究领域的病原分离鉴定、诊断方法、分子流行病学及兽用生物制品研发等方面的最新进展及成果。主题刊发表后引起了同行及读者的广泛好评。2019年末,突如其来的新型冠状病毒肺炎疫情给人类的生活、工作与学习带来了巨大影响,也让病毒、变异、核酸检测、疫苗等词汇快速进入大众视野。“同一健康”“公共卫生”“生物安全”在社会层面被广泛认知,兽医微生物学对公共卫生和人类健康的重要作用愈加突显。在这种背景下,时隔五年,《微生物学通报》与兽医微生物学专业委员会再次达成共识,联合推出“兽医微生物学主题刊”第二季,旨在系统梳理我国兽医微生物学研究领域的新理论、新技术与新产品,搭建兽医微生物学科研工作者学术思想与科研成果的交流与展示平台,推进学科发展与繁荣,为健康中国、健康世界贡献智慧与力量。

关键词：新型冠状病毒肺炎疫情兽医微生物学同一健康公共卫生健康中国

Modern agriculture and One Health

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贫困所致传染病(英文) 2024年第5期13卷 1-5页

作者： Zhang Guangzhi Qiu Yu Boireau Pascal Zhang Yinghui Ma Xin Jiang Hui Xin Ting Zhang Maodun Tadesse Zelalem Wani Nisar Ahmad Song Junxia ding jiabo Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China Animal Production and Health Division Food and Agriculture Organization of the United Nations Rome Italy ANSES Laboratoire de Santé Animale Maisons-Alfort France Institute of Veterinary Drug Control Beijing China University of California Berkeley CA-3200 USA Reproductive Biotechnology Center Dubai UAE

Background The development of modern agriculture has significantly contributed to improving global food security and safety, alleviating poverty, and enhancing human health and livelihoods. However, the rapid advancement of modern agriculture has also brought about various challenges that limit its sustainable development. This commentary aims to discuss these issues through the One Health lens, and provide valuable insights for balancing modern agricultural activities with the need to protect and promote the health of all the *** text This commentary explores the multifaceted impacts of modern agriculture on social development, as well as the associated various health challenges and environmental impacts within the One Health framework. Key issues include ecosystem degradation, increased risk of interspecies disease transmission like zoonoses, reverse zoonoses, and vector-borne diseases, and the escalated threat of antimicrobial resistance due to intensified agricultural production and increased antimicrobial use. To address these challenges, this commentary outlines potential solutions anchored in the development and implementation of modern technologies and good agricultural practices, such as precision farming, integrated pest management, biosecurity measures, vaccination programs, as well as surveillance and early detection of health *** Good agricultural practices supported by scientific and technological advancements are essential for aligning productivity with the One Health vision, ensuring the health and resilience of all the sectors. Enhancing stakeholder education, strengthening regulatory frameworks, and providing supportive policies and infrastructure for farmers to adopt sustainable practices are crucial for the long-term viability of agrifood systems. The Food and Agriculture Organization of the United Nations plays a pivotal role in guiding this sustainable transformation through the One Health approach.

关键词： Modern agriculture One Health Ecosystem Interspecies disease transmission Antimicrobial resistance Sustainable agrifood systems transformation

The prevention and control of domesticated animal brucellosis

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Biosafety and Health 2021年第4期3卷 197-201页

作者： Menachem Banai Hui Jiang Xiaowei Peng Yu Feng Hai Jiang jiabo ding Department of Bacteriology Kimron Veterinary InstitutePO Box 12Bet Dagan 50250Israel China Institute of Veterinary Drug Control Beijing 102600China Chinese Center for Disease Control and Prevention Beijing 102206China

Brucellosis is a zoonotic disease caused by a number of bacteria in the genus *** animals,classical Brucella species naturally affiliate with mammals in which they cause epididymitis and orchitis in males and abortion storms in pregnant *** Brucella proliferates to a vast number of free organisms in the fetal organs and fluids,abortion plays a key mechanism in disease spread in ***,***,and *** construct the three most severe zoonotic species and their role in human brucellosis stems from their association with domesticated farm animals in which they strive and transmit the *** we review the relevant literature on genus Brucella,vaccination and diagnostic ***,the prevention and control programs based on the combination of vaccination coverage of the population with differentiating serological diagnosis of the infected from vaccinated animals(DIVA)and herd clearance by a test and cull strategy using validated serological tests were discussed.

关键词： Brucellosis Vaccine Diagnosis Prevention and control

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采用双重投影法的椭球面日晷投影

武汉测绘科技大学学报 2000年第2期25卷 183-185页

作者：丁佳波中国航海图书出版社天津市上海道102号300450

研究了从地球椭球面描写到海图平面的采用双重投影法的日晷投影方程式 ,并阐明了椭球面日晷投影的

关键词：大椭圆共焦双曲线椭球面日晷投影地图投影

Comparison among three different methods on detecting paratuberculosis in Shandong Province,China

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Comparison among three different methods on detecting paratu...

第五届全国人畜共患病学术研讨会

作者： Yong Peng Yu Feng Hui Jiang Liangquan Zhu jiabo ding China Institute of Veterinary Drug Control College of Animal Science and Veterinary Medicine Shandong Agricultural University

Paratuberculosis is a severe globally-distributed zoonosis,caused by the Mycobacterium avium ***(MAP).Cattle,goat,and the sheep are the main hosts of the agent,human as ***,there is no single test can clearly demonstrate the presence of *** fecal culture method is the most reliable way to detect the MAP,it requires 3-4 months to identify

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低致病性禽流感病毒对养禽业的危害

中国家禽 2006年第6期28卷 1-3页

作者：刁有祥丁家波山东农业大学动物科技学院山东泰安271018 中国兽医药品监察所北京100081

文章对致病性禽流感病毒的特性、对养禽业的危害以及致病性禽流感的预防作了综合性叙述,重点对低致病性禽流感病毒的危害、免疫抑制性、毒性的变异性作了叙述,对生产、科研具有一定的参考意义。

关键词：低致病性禽流感病毒养禽业高致病性禽流感病毒流行性感冒病毒危害禽流行性感冒病毒感染死亡率症状消化道

副结核分枝杆菌感染的免疫应答与检测方法研究进展

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微生物学报 2016年第10期56卷 1530-1536页

作者：张振常维山丁家波山东农业大学动物科技学院山东泰安270018 中国兽医药品监察所北京100081

副结核分枝杆菌常引起感染牛的产奶量下降、持续性消瘦、顽固性下痢甚至死亡,给畜牧业带来了巨大的经济损失。牛通常在幼年期经口感染该菌并具有一个较长的亚临床期,后期才表现出临床症状,感染前期以细胞免疫为主并伴随着间歇排菌,经过... 详细信息

副结核分枝杆菌常引起感染牛的产奶量下降、持续性消瘦、顽固性下痢甚至死亡,给畜牧业带来了巨大的经济损失。牛通常在幼年期经口感染该菌并具有一个较长的亚临床期,后期才表现出临床症状,感染前期以细胞免疫为主并伴随着间歇排菌,经过一个2到5年的亚临床期后体液免疫应答增强,同时排菌量明显增加。目前对牛副结核病常用的检测方法有病原学检测方法,基于细胞免疫反应和基于体液免疫反应的检测方法。由于不同方法的反应原理不同,加之副结核分枝杆菌感染动物的特定免疫应答规律,在某一时间内各方法之间敏感性差异较大。本文简要阐述了牛副结核分枝杆菌的传播途径以及牛感染后的免疫应答特点,对牛副结核病的常见诊断方法进行了综述。

关键词：副结核分枝杆菌牛副结核病免疫应答检测方法

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常用消毒剂对布鲁氏菌疫苗株的灭菌效果评估

微生物学通报 2021年第3期48卷 1033-1040页

作者：冯宇王芳丁家波蒋卉中国兽医药品监察所北京102600

【背景】布鲁氏菌是一种重要的人畜共患胞内寄生菌,是引起动物和人发生布鲁氏菌病(布病)的病原体。高效消毒剂杀灭环境中的布鲁氏菌是防控布病的关键措施。【目的】评价市面上常用消毒剂对布鲁氏菌的灭菌效果及差异。【方法】选用市面上... 详细信息

【背景】布鲁氏菌是一种重要的人畜共患胞内寄生菌,是引起动物和人发生布鲁氏菌病(布病)的病原体。高效消毒剂杀灭环境中的布鲁氏菌是防控布病的关键措施。【目的】评价市面上常用消毒剂对布鲁氏菌的灭菌效果及差异。【方法】选用市面上9种不同类型的常用消毒剂,以高、中、低3种不同使用浓度分别研究其对牛种、羊种和猪种布鲁氏菌的杀灭效果。同时根据消毒剂的有效浓度通过最小抑菌浓度实验确定各消毒剂的最小抑菌浓度。【结果】除苯酚外,其余8种消毒剂,包括聚维酮碘、癸甲溴铵、苯扎溴铵、戊二醛、复合季铵盐、乙醇和2种商品化消毒剂等在推荐使用浓度下5 min内均能完全杀灭布鲁氏菌,碘酸制剂在0.1%低使用浓度下细菌存活率为0.000039%-0.000095%。苯酚按0.5%的推荐剂量,作用5 min后细菌存活率为0.000144%-0.000183%,20 min后,细菌存活率为0.000138%-0.000193%;按1%的使用剂量作用5 min后,猪种布鲁氏菌存活率为0.000125%,作用20min后的存活率下降至0.000038%。最小抑菌浓度研究结果表明,季铵盐类消毒剂其最小抑菌浓度大致在0.01563%-0.03125%之间,其余消毒剂的最小抑菌浓度结果与消毒剂最低推荐使用浓度基本一致。研究同时发现,不同种属布鲁氏菌对同一消毒剂的敏感性也存在一定差异。【结论】研究结果为生产实践中科学选择布鲁氏菌消毒剂提供了借鉴。

关键词：布鲁氏菌消毒剂灭菌效果

Development of a multiplex PCR method for distinguishing the reference strain Brucella melitensis 63/9

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Development of a multiplex PCR method for distinguishing the...