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A Biosurfactant-containing TSD Strategy to Modify Bovine Pericardial Bioprosthetic Valves for Anticalcification

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Chinese Journal of Polymer Science 2023年第1期41卷 51-66页

作者： Cai-Yun Gao Gang Wang Lin Wang Qun-Song Wang Han-Cheng Wang Lin Yu Jian-Xiong Liu Jian-dong ding State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai200438China R&D Center Lifetech Scientific(Shenzhen)Co.Ltd.Shenzhen518057China R&D Center Lifevalve Medical Scientific Co.Ltd.Shenzhen518057China

Bioprosthetic heart valves(BHVs)are important for transcatheter valve *** commercial BHVs on the market are basically porcine or bovine pericardium(BP)crosslinked with glutaraldehyde(GA).Simply applying GA to BHVs can enhance mechanical stability,but cannot alleviate in vivo *** this work,we developed a two-step decellularization(TSD)strategy to modify this biomacromolecular network,in which BP was post-treated,as the second step of decellularization,with a mild biosurfactant n-dodecyl-β-D-maltoside in a mixture of isopropanol and phosphate-buffered saline after the first step of traditional decellularization and GA *** TSD-treated BP exhibited not only low cytotoxicity and excellent mechanical properties in vitro,but also low immune responses and significant anticalcification in *** 60 days of subcutaneous implantation in the back of Wistar rats,the calcium content was,as quantified with an inductively coupled plasma optical emission spectrometer,only 1.1µg/mg compared to 138.6µg/mg in the control group without the *** addition,collagen fibrils were observed with field emitting scanning electron microscopy(SEM),and the morphology and composition of the calcified sites resulting from in vivo biomineralization were studied with SEM with energy dispersive spectroscopy and also X-ray *** study proposes a facile yet effective anticalcification strategy for the modification of the bovine pericardial bioprosthetic heart valve,a natural biomacromolecular network.

关键词： Heart valve Anticalcification Collagen In vivo biomineralization Transcatheter aortic valve implantation(TAVI) Extracellular matrix Biomacromolecular network Biosurfactant Bovine pericardium

Improved cell performance and sulphur tolerance using A-site substituted Sr_(2)Fe_(1.4)Ni_(0.1)Mo_(0.5)O_(6-δ)anodes for solid-oxide fuel cells

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Clean Energy 2023年第1期7卷 70-83页

作者： Haixia Li Wanhua Wang Jie Lin Ka-Young Park Taehee Lee Andreas Heyden dong ding Fanglin Chen Department of Mechanical Engineering University of South CarolinaColumbiaSC29208USA Department of Chemical Engineering University of South CarolinaColumbiaSC29208USA Energy&Environmental Science and Technology Idaho National LaboratoryIdaho FallsID83401USA

Solid-oxide fuel cells(SOFCs)offer great promise for producing electricity using a wide variety of fuels such as natural gas,coal gas and gasified carbonaceous solids;however,conventional nickel-based anodes face great challenges due to contaminants in readily available fuels,especially sulphur-containing ***,the development of new anode materials that can suppress sulphur poisoning is crucial to the realization of fuel-flexible and cost-effective *** this work,La_(0.1)Sr_(1.9)Fe_(1.4)Ni_(0.1)Mo_(0.5)O_(6-δ)(LSFNM)and Pr_(0.1)Sr_(1.9)Fe_(1.4)Ni_(0.1)Mo_(0.5)O_(6-δ)(PSFNM)materials have been synthesized using a sol-gel method in air and investigated as anode mater-ials for *** nanoparticle-decorated ceramic anodes were obtained by the reduction of LSFNM and PSFNM in H_(2)at 850℃,forming a Ruddlesden-Popper oxide with exsolved FeNi3 bimetallic *** electrochemical performance of the Sr_(2)Fe_(1.4)Ni_(0.1)Mo_(0.5)O_(6-δ)ceramic anode was greatly enhanced by La doping of A-sites,resulting in a 44%decrease in the polarization resistance in reducing *** maximum power densities of Sr-and Mg-doped LaGaO_(3)(LSGM)(300μm)electrolyte-supported single cells with LSFNM as the anode reached 1.371 W cm^(-2)in H_(2)and 1.306 W cm^(-2)in 50 ppm H_(2)S-H_(2)at 850℃.Meanwhile,PSFNM showed improved sulphur tolerance,which could be fully recovered after six cycles from H_(2)to 50 ppm H_(2)S-H_(2)*** study indicates that LSFNM and PSFNM are promising high-performance anodes for SOFCs.

关键词： A-site doping perovskite Ruddlesden-Popper structure alloy nanoparticles polarization resistance solid oxide fuel cells

An Injectable Hydrogel with or without Drugs for Prevention of Epidural Scar Adhesion after Laminectomy in Rats

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Chinese Journal of Polymer Science 2016年第2期34卷 147-163页

作者： Lin Chen Xiang-qian Li Lu-ping Cao Xi-lei Li Jun-rong Meng 董健俞麟 Jian-dong ding State Key Laboratory of Molecular Engineering of Polymers&Department of Macromolecular Science Fudan UniversityShanghai 200433China Department of Orthopaedic Surgery Zhongshan HospitalFudan UniversityShanghai 200032China

Epidural scarring occurs inevitably in the defect after spinal laminectomy, and thus how to prevent or reduce it becomes a challenging topic. In the present study, an injectable hydrogel and its dexamethasone （DEX）-loaded hydrogel systems were adopted to prevent epidural scarring in a postlaminectomy rat model. The hydrogel system composed of poly（D,L-lactic acid-co-glycolic acid）-poly（ethylene glycol）-poly（D,L-lactic acid-co-glycolic acid）（PLGA-PEG-PLGA） triblock copolymers was a free-flowing sol at room temperature, and spontaneously turned into a semi-solid gel at body temperature. A lumbar 3 total laminectomy was performed on Sprague Dawley （SD） rats, and the efficacy of the injectable hydrogel with or without drugs in preventing epidural scar formation was evaluated via the gross anatomical observation and histological examination at one month post-surgery. The results demonstrate that the use of hydrogel alone reduced epidural scarring significantly, whereas the efficacy of the DEX-loaded hydrogels presented an irregular dose-dependency of drug and even the inappropriate drug doses resulted in the negative results. Therefore, the present study confirms that the PLGA-PEG- PLGA hydrogel holds potential as a barrier device to decrease peridural scarring, and reveals that the sustained delivery of the steroid hormone DEX to prevent surgery-related adhesions in the laminectomy defect is complex. Moreover, our in vivo studies also remind the researchers to pay attention to the irregular dose-dependency of the hormone drugs.

关键词： Thermo-reversible hydrogel Epidural scarring Laminectomy Steroid hormone.

Temporal-Spatial Variation of Surface Suspended Matter and Controlling Factors in the Inner Shelf of the East China Sea in Winter

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Journal of Ocean University of China 2019年第1期18卷 9-19页

作者： MIN Jianxiong LI Guangxue ding dong QIAO Lulu MA Yanyan YANG Gang ZHANG Yaqi ZHANG Lei LI Shuhao Shanghai Waterway Engineering Design and Consulting Co. Ltd. College of Marine Geosciences Ocean University of China Key Laboratory of Submarine Geosciences and Prospecting Techniques Ministry of Education Ocean University of China

Based on the observed surface suspended matter in the East China Sea in February 2007 and June 2015, an empirical model was established using L1 b's band 4 data to retrieve surface suspended matter from the Moderate Resolution Imagine Spectroradiometer Terra imagery. The squared correlation coefficient is 0.8358, and the root mean square error is 0.4285 mg L-1. The model reflects the distribution characteristics of surface suspended matter in the inner shelf of the East China Sea. In this paper, the satellite images of the study area were retrieved in January from 2001 to 2015, and the monthly distribution of surface suspended matter were obtained. The inter-annual distribution of the study area is similar, and the concentration of surface suspended matter is higher near the shore than offshore. A large amount of surface suspended matter is transported southeast under the influence of Zhejiang and Fujian coastal current and Taiwan warm current. Only a small amount of surface suspension can reach the Kuroshio area. The surface suspended matter concentration changes obviously near the estuary because of the effect of differences in the flux of the Yangtze River. Meanwhile, winter monsoon, temperature front, El Ni?o events, and other factors affect the distribution of surface suspended matter in 100 m isobath to coastal water but minimally influence the distribution in 100 m isobath to deep sea.

关键词： inner shelf of the East China Sea Moderate Resolution Imagine Spectroradiometer surface suspended matter monthly distribution inter-annual variation

China-Russia Ten-thousand-mile Tea Road

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International Understanding 2014年第4期 34-37页

作者： ding dong

From November 5 to 14,2014,a nine-member European NGO study group visited China at the invitation of *** members of the group came from Russian and Austrian NGOs and think tanks,some of whom are senior China *** help them get a comprehensive idea of China’s development,in Beijing,Deputy SecretaryGeneral Zheng Yao briefed the group on

关键词： Russia thousand invitation visited think heritage

Waiting for You Under the Camphor Tree

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China Book International 2024年第1期 108-110页

作者： ding dong People's Congress of Zhangjiagang City

This book is a collection of essays rich in local flavor and everyday life,both documentary and *** author,extracts what he has seen,heard,thought,and realized in his life journey,reflecting on the past and the present,empathizing with others,and deeply interpreting the history,authenticity,and culture of *** author presents the development and life history of the common people of Zhangjiagang from multiple levels,aspects,and angles.

关键词： everyday authentic interpreting

The role of AtGPDHc2 in regulating cellular redox homeostasis of Arabidopsis under salt stress

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Journal of Integrative Agriculture 2019年第6期18卷 1266-1279页

作者： ZHAO Ying LIU Meng WANG Feng ding dong ZHAO Chang-jiang HE Lin LI Zuo-tong XU Jing-yu Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province/Daqing Key Laboratory of Straw Reclamation Technology Research and Development/College of Agriculture Heilongjiang Bayi Agricultural University

Plants glycerol-3-phosphate dehydrogenase(GPDH)catalyzes the formation of glycerol-3-phosphate,and plays an essential role in glycerolipid metabolism and stress *** the present study,the knock-out mutants of cytosolic GPDH(AtGPDHc2)and wild-type Arabidopsis plants were treated with 0,50,100,and 150 mmol L–1 NaCl to reveal the effects of AtGPDHc2 deficiency on salinity stress *** fluctuation in redox status,reactive oxygen species(ROS)and antioxidant enzymes as well as the transcripts of genes involved in the relevant processes were *** the presence of 100 and 150 mmol L–1 NaCl treatments,AtGPDHc2-deficient plants exhibited a pronounced reduction in germination rate,fresh weight,root length,and overall ***,loss of AtGPDHc2 resulted in a significant perturbation in cellular redox state(NADH/NAD+and AsA/DHA)and consequent elevation of ROS and thiobarbituric acid-reactive substances(TBARS)*** elevated ROS level triggered substantial increases in ROS-scavenging enzymes activities,and the up-regulated transcripts of the genes(CSD1,sAPX and PER33)encoding the antioxidant enzymes were also *** addition,the transcript levels of COX15,AOX1A and GLDH in gpdhc2 mutants decreased in comparison to wild-type plants,which demonstrated that the deficiency of AtGPDHc2 might also has impact on mitochondrial respiration under salt ***,this work provides some new evidences on illustrating the roles of AtGPDHc2 playing in response to salinity stress by regulating cellular redox homeostasis,ROS metabolism and mitochondrial respiration.

关键词： Arabidopsis thaliana glycerol-3-phosphate dehydrogenase(GPDH) redox homeostasis reactive oxygen species(ROS) respiration

Convexity and Positivity of Bivariate Quadratic Bézier Surfaces over a Rectangle

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Journal of Shanghai University(English Edition) 2001年第1期5卷 20-28页

作者： ding You dong School of Computer Engineering and Science, Shanghai University, Shanghai 200072, China School of Computer Engineering and Science Shanghai University Shanghai China

With the help of several discriminants about the zero points of a quartic polynomial, the sufficient and necessary conditions for the positivity and nonnegativity of the quartic polynomial over an interval I(-∞,+∞) was derived. Based on these conclusions, the sufficient and necessary conditions for the positivity and convexity of the 2×2 Bézier surface over a rectangle were obtained. A simple sufficient condition was deduced also and finally several examples were given.

关键词： Bézier surface convexity positivity discriminant

The chimeric gene atp6c confers cytoplasmic male sterility in maize by impairing the assembly of the mitochondrial ATP synthase complex

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Molecular Plant 2022年第5期15卷 872-886页

作者： Huili Yang Yadong Xue Bing Li Yanan Lin Haochuan Li Zhanyong Guo Weihua Li Zhiyuan Fu dong ding Jihua Tang National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhouChina The Shennong Laboratory ZhengzhouChina

Cytoplasmic male sterility(CMS)is a powerful tool for the exploitation of hybrid heterosis and the study of signaling and interactions between the nucleus and the cytoplasm.C-type CMS(CMS-C)in maize has long been used in hybrid seed production,but the underlying sterility factor and its mechanism of action remain *** this study,we demonstrate that the mitochondrial gene atp6c confers male sterility in CMS-C *** ATP6C protein shows stronger interactions with ATP8 and ATP9 than ATP6 during the assembly of F1F0-ATP synthase(F-type ATP synthase,ATPase),thereby reducing the quantity and activity of assem-bled F_(1)F_(o)-ATP *** contrast,the quantity and activity of the F1'component are increased in CMS-C *** F1F0-ATP synthase activity causes accumulation of excess protons in the inner membrane space of the mitochondria,triggering a burst of reactive oxygen species(ROS),premature programmed cell death of the tapetai cells,and pollen ***,our study identifies a chimeric mitochondrial gene(ATP6C)that causes CMS in maize and documents the contribution of ATP6C to F1F0-ATP synthase assembly,thereby providing novel insights into the molecular mechanisms of male sterility in plants.

关键词： maize cytoplasmic male sterility atp6c ATP synthase mitochondria