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Helicobacter pylori Virulence Genes cagA, babA2, and vacA Detection in Dyspeptic Patients from Burkina Faso

American Journal of Molecular Biology 2023年第3期13卷 141-155页

作者： Tegwinde Rebeca Compaore Kalifou Traore Nômawendé Ines Compaore Lassina Traore Sylvie Zida Serge Theophile Soubeiga dinanibe kambire Jean Claude R. P. Ouedraogo Aminata Dickel Sidibe Yasmine Astrid Sana Tani Sagna Wendkuuni Florencia Djigma Henri Gautier Ouedraogo Jacques Simpore Département Biomédical et Santee Publique Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie et de Génétique Moléculaire (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Clinique El Fateh-Suka Service de médecine et de spécialités médicales Ouagadougou Burkina Faso Département de Médecine et de Pharmacopée Traditionnelle Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso

The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the interaction between bacterial virulence, host genetics, socio-demographic and environmental factors. This study aimed to characterize Helicobacter pylori virulence genes and the associated behavioral factors among dyspeptic patients in Burkina Faso. Two hundred and fifty (250) stool samples were collected from patients with dyspepsia seen at health centers in Ouagadougou, Burkina Faso. Bacterial deoxyribonucleic acid (DNA) was extracted using a commercial kit. Virulence genes were detected using conventional multiplex Polymerase Chain Reaction with specific primers. The overall prevalence of Helicobacter pylori of the 250 participants was 91.20%. CagA virulence gene was present among 20.19% of individuals, while babA2 and vacA were detected respectively among 9.65% and 67.54% of the population positive for Helicobacter pylori. Among vacA subtypes, vacAs1 was the most frequent, with 39.04%, followed by vacAi1 (19.74%), vacAi2 (17.54%), and vacAs2 with 10.96%. Regarding vacAm1 and vacAm2, they were less frequent at 6.14% each. “Handwashing three times or less per day” significantly increased the risk of having vacAi2 allele and H. pylori rRNA16s, with p-values of 0.013 and 0.020, respectively. The consumption of non-tap water increases the risk of carrying the cagA virulence gene. Additionally, H. pylori-positive patients living with more than four (4) people in their household had about two times the risk of having the vacAs1 allele. The present study shows the detection of Helicobacter pylori cagA, vacA subtypes, and babA2 by stool a PCR method in Burkina Faso. The strong association between sanitary habits and virulence factors depicts the composite interaction between ecological factors, gastric mucosa, and bacteria. Therefore, the synergic action of these factors should be considered when aiming for bacterial eradication and gastric pathology cure.

关键词： Helicobacter pylori Stool cagA babA2 vacA

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Drivers of the Chemical Quality of Market Gardening Soils in the Urban and Peri-Urban Environment of Bobo-Dioulasso (Burkina Faso): Impact of Fertilizers Sources and Sites Location

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Journal of Agricultural Chemistry and Environment 2023年第1期12卷 1-15页

作者： Fabèkourè Cédric kambire Sheick Ahmed Khalil S. B. Sangare R. Adèle Ouedraogo Adama Zanga Ouattara Institute for Research in Applied Sciences and Technologies (IRSAT)/National Centre for Scientific and Technological Research (CNRST) Bobo-Dioulasso Burkina Faso Nazi Boni University/Laboratory of Study and Research on Soil Fertility (LERF) Bobo-Dioulasso Burkina Faso

Urban and peri-urban agriculture plays a key role by providing many goods and services. In particular, it provides diversified food and employment for vulnerable groups (youth and women). However, it often involves negative externalities due to non-conventional soils fertility management practices. This study aimed to investigate the chemical quality of soils over six (06) sites of the market gardening area of Bobo-Dioulasso (Burkina Faso) as affected by fertilizers uses and sites location. Thirty (30) representative market gardening farms, located in urban, semi-urban and rural areas, were randomly selected from a baseline survey database. Within each farm, composite soil samples made up of 3 individual cores were taken over the 0 - 15 cm soil depth for determining soils carbon, total nitrogen, available phosphorus contents and pH-water. These data were normalized and summarized to compute a synthetic Soil Fertility Index (SFI). The data processing was focused on a Principal Component Analysis and an Ascendant Hierarchical Classification in order to make a typology of the vegetable farms. Fertilizers management effects on soils quality were compared through Variance Analysis (ANOVA) following a GLM procedure in Rstudio software. As main results, soils chemical parameters, except for available K, were affected by the location of the sites. Soils in urban farms are less acidic (on average pH = 6.9), while semi-urban and rural sites (Samadeni, Nakaguana) have more acidic soils. However, the latter site had the highest values of C and N. Moreover, the long-term application of organic matter sources results in improving of the chemical quality of the market garden soil. The SFI is positively correlated with the rate of applied organic fertilizers, and the cultivation duration. On the other hand, soil quality tends to decrease with the expansion of the area, due to a dilution effect of the organic fertilizer doses. All these results suggest that there is a real scope to

关键词： Gardening Soil Fertility Index Chemical Fertility Bobo-Dioulasso

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Incisional Hernias: Risk Factors and Treatment in a Context of Limited Resources

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Surgical Science 2017年第10期8卷 451-456页

作者： Souleymane Ouedraogo Salam Ouedraogo Jean-Luc kambire Mamadou Windsouri Mamadou T. Traore Maurice Zida , Si Simon Traore Department of Surgery University Ouaga 1 Pr Joseph Ki Zerbo Ouagadougou Burkina Faso Department of General and Digestive Surgery University Ouaga 1 Pr Joseph Ki Zerbo Ouagadougou Burkina Faso

Background: The purpose of this study was to describe the etiologic factors and therapeutic modalities of incisional hernias in the department of surgery at the regional hospital of Tenkodogo, in Burkina Faso. Methods: It is a cross sectional study covering the period from 2010 to 2012. Sampling was comprehensive, including all the patients operated for incisional hernias during this period. Results: Fifty-four patients were included. Their average age was 22.3 years. There were 35 (64.8%) males and 19 (35.2%) female patients. We counted 28 large-size incisional hernias, 14 medium-size incisional hernias and 12 incisional hernias of small size. The average time from the first surgery to the diagnosis of incisional hernias was 8 months. Generalized acute peritonitis (44.4%) and acute intestinal obstructions (22.2%) were the main indications of initial surgical interventions. Vertical midline incisions have been involved in 37 cases (68.5%). Therapeutically, two surgical techniques have been used: open suture repair and open mesh repair. Forty-two patients (77.8%) were treated by open suture repair. Twelve patients (22.2%) benefited from open mesh repair. Post-operative complications were noted in 2 patients. Nine patients (16.7%) presented a recurrence after 12 months. Conclusion: In the rural area of Burkina Faso, incisional hernias occur in young patients. Emergencies with abdominal Infections are the main operative indications. Prostheses are relatively high cost and less accessible. The recidivism rate is high.

关键词： Incisional Hernia Abdominal Wall Closure Surgical Treatment Burkina Faso

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Microbiological Quality Assessment of Mutton in the Saaba District in Ouagadougou, Burkina Faso

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Advances in Microbiology 2024年第10期14卷 532-543页

作者： Dissinviel Stéphane Kpoda Assiètta Ouattara Ange Irénée Toe Dinanibè kambire Serge Touwendsida Bagre Hawa Kagambega Ibrahim Barry Iliassou Mogmenga Cheik Amadou Tidiane Ouattara Sidiki Aboubakar Ouattara Ziniar University Center Joseph Ki-Zerbo University Ouagadougou Burkina Faso Laboratory of Microbiology and Microbial Biotechnologies Joseph Ki-Zerbo University Ouagadougou Burkina Faso Tenkodogo University Center Thomas Sankara University Ouagadougou Burkina Faso National Center for Scientific and Technological Research Ouagadougou Burkina Faso National School of Animal and Livestock Health Ouagadougou Burkina Faso Banfora University Center Nazi BONI University Bobo-Dioulasso Burkina Faso

Introduction: This study was carried out to assess the quality of sheep meat sold to consumers in Saaba municipality. Methods: A preliminary survey consisted of assessing hygiene and sampling meat in butchers’ stores. To achieve this, 100 sales outlets were surveyed for their hygiene conditions. 25 mutton meat samples were sampled in the three villages in Saaba district and analyzed using conventional microbiological techniques. The analysis consisted of determining the microbiological characteristics. Results: According to the results of this study, 100% of the butchers surveyed were men of the region. Among these men, 13% had secondary education, 46% had primary education and 41% were illiterate. Of the 100 retail outlets visited, 96% of the sellers were unaware of the hygiene rules and the dangers of microorganisms. The microbiological analysis of the samples revealed that 100% of the meat was of unsatisfactory microbiological quality. The average contamination rate by total aerobic mesophilic flora, total coliforms, Staphylococcus aureus and yeasts and molds was 8.93 × 106, 3.12 × 105, 3.69 × 106, and 6.74 × 103 CFU/g respectively. No Salmonella strain was detected in any of the samples analyzed. Conclusion: Our results pointed out the unsatisfactory safety quality of the sheep meat sold in the sheep meat and good hygiene practices.

关键词： Sheep Meat Microbiological Analyses Ouagadougou Fecal Coliforms Mesophilic Flora

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Evaluation of Two Serological Screening Kits for Hepatitis C Virus Infection at the Regional Blood Transfusion Center of Ouagadougou, Burkina Faso

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Advances in Infectious Diseases 2020年第4期10卷 216-227页

作者： Arzouma Paul Yooda Koumpingnin Nebie Juliette Tranchot-Diallo Salam Sawadogo Moutanou Modeste Judes Zeye Abdoul-Guaniyi Sawadogo Abdou Azaque Zoure Dinanibè kambire Serge Sawadogo Seimbou Zalla Yetema Dieudonné Yonli Abibou Simpore Sibiri Nana Ashmed Chèickh Bachirou Nana Anita Pierrette Siritie Fiffou Yougbare Sonia Ba-Nébhane Sontie Alain Konseybo Jean Etienne Koanda Oury Sanou Jacques Simpore National Blood Transfusion Center (NBTC) Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) Joseph KI-ZERBO University Ouagadougou Burkina Faso Training and Research Unit/Science and Technology (TRU/ST) Nazi-BONI University Bobo-Dioulasso Burkina Faso Research Institute in Health Sciences (IRSS/CNRST) Ouagadougou Burkina Faso

Introduction: In Burkina Faso, screening for hepatitis C virus in blood donations is made using sensitive ELISA (Enzyme Linked Immuno Sorbent Assay) type kits. However, no confirmation of the positive results obtained with these kits is made before their notification to the blood donors due to the high costs of the confirmation kits of immunoblots type. Objective: Evaluate two rapid kits against one immunoblot kit in order to determine the most efficiency which will be proposed as an alternative for the confirmation of ELISA tests in the socio-economic context of Burkina Faso. Material and Methods: The study was carried out using a panel of 72 sera, of which 22 were positive for anti-HCV antibodies and 50 were negative. The sera were tested using the Monolisa® HCV Ag-Ab ULTRA kit and confirmed with the DECISCAN HCV Plus kit. The panel was then tested with the SD BIOLINE HCV kit and the HCV TRI-DOT kit and the results obtained were evaluated against those of the DECISCAN HCV Plus kit used as “gold standard”. Results: Compared to the DECISCAN HCV Plus kit, the HCV TRI-DOT kit exhibited a sensitivity and specificity of 100% and the SD BIOLINE HCV kit a sensitivity of 86.36% and a specificity of 100%. Conclusion: Based on the results recorded by the HCV TRI-DOT kit, it would be best suited to the sero-epidemiological context of blood donors from the National Blood Transfusion Center and could be proposed as an alternative for confirmation of ELISA tests.

关键词： HCV Sensitivity Specificity Burkina Faso

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