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BIOCELL 2019年第1期43卷 21-28页

作者： Nesrine EBRAHIM Eman EHSAN Eman Abd EL GHANY dina sabry Ashraf SHAMAA Histology and Cell Biology Department Faculty of MedicineBenha UniversityBenhaEgypt Pediatrics Department Faculty of MedicineCairo UniversityGizaEgypt Medical Biochemistry and molecular biology Department Faculty of MedicineCairo UniversityGizaEgypt Surgical and Radiology Department Faculty of Veterinary MedicineCairo UniversityGizaEgypt

The neonatal hypoxic-ischemic encephalopathy(HIE)is an important cause of neurological morbidity and mortality in *** therapy is considered a promising method for treating severe neurological disorders such as this *** cells have the capacity for self-renewal and differentiation into certain cell *** present study was aimed to find out the most beneficial route of bone marrow-derived mesenchymal stem cells(BMSCs)administration for the attenuation of experimentally induced HIE in neonatal *** neonatal rats were divided randomly into four *** 1:control *** 2:rats were exposed to bilateral ligation of cephalic *** 3:rats were exposed to bilateral ligation of cephalic arteries and then underwent intravenous(IV)BMSC *** 4:rats were exposed to bilateral ligation of cephalic arteries and then underwent intracerebroventricular(ICV)BMSC *** animals were evaluated by(a)neurobehavioral tests;(b)histopathology,i.e.,histological and immuno-histochemical studies;and(3)gene expression *** BMSC treated groups(3 and 4)showed improvement in neurobehavioral tests,histopathological studies,and gene expression,as compared to non-injected lesioned rats(Group 2)with better improvement in Group 4(ICV injections)than in Group 3(IV injections).

关键词： Cerebral cortex Neonatal hypoxic ischemia Stem cells

Assessment of heme oxygenase-1 （HO-1） activity in the cavernous tissues of sildenafil citrate-treated rats

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Asian Journal of Andrology 2007年第3期9卷 377-381页

作者： M. Talaat Abdel Aziz M. Farid Al-Asmar Taymour Mostafa Hazem Atta Laila Rashed dina sabry Shedeed Ashour Ahmed T. Abdel Aziz Molecular Biology Unit Medical Biochemistry Department Faculty of Medicine Cairo University Cairo 11553 Egypt Medical Biochemistry Department Faculty of Medicine Ain Shams University Cairo Egypt Andrology Department Faculty of Medicine Cairo University Cairo 11553 Egypt

Aim： To assess heine oxygenase-1 （HO-1） activity in the cavemous tissue of sildenafil citrate-treated rats. Methods： One hundred and ninety-two Sprague-Dawley male rats, divided into four equal groups, were investigated. Group 1, the control group, received regular animal chow; group 2 received sildenafil citrate by intragastric tube; group 3 received sildenafil and HO inhibitor （zinc protoporphyrin, ZnPP）; and group 4 received sildenafil and nitric oxide synthase （NOS） inhibitor L-nitroarginine methyl ester （L-NAME）. Twelve rats from each group were killed after 0.5 h, 1 h, 2 h and 3 h of drug administration. Then HO-1 activity, cGMP levels and NOS enzymatic activity in the cavernous tissues were estimated. Results： In cavemous tissue, HO-1 activity, NOS enzymatic activity and cGMP concentration increased significantly in sildenafil-treated rats compared to other groups throughout the experiment. Rats receiving either HO or NOS inhibitors showed a significant decrease in these parameters. HO- 1 cavemous tissue activity and NOS enzymatic activity demonstrated a positive significant correlation with cGMP levels （r = 0.646, r = 0.612 respectively; P 〈 0.001）. Conclusion： The actions of PDE5 inhibitor sildenafil citrate in the cavernous tissue are partly mediated through the interdependent relationship between both HO-1 and NOS activities.

关键词： erectile dysfunction heme oxygenase sildenafil citrate nitric oxide synthase carbon monoxide

Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury？

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Neural Regeneration Research 2017年第12期12卷 2050-2058页

作者： Nesrine Salem Mohamed Y.Salem Mohammed M.Elmaghrabi Moataz A.Elawady Mona A.Elawady dina sabry Ashraf Shamaa Abdel-Haleem H.Elkasapy Noha Ibrhim Azza Elamir Department of Histology and Cell Biology Faculty of Medicine Banha University Banha Egypt Department of Neurosurgery Faculty of Medicine Banha University Banha Egypt Department of Community Medicine Faculty of Medicine Banha University Banha Egypt Department of Medical Biochemistry Faculty of Medicine Cairo University Giza Egypt Department of Surgery and Radiology Faculty of Veterinary Medicine Cairo University Giza Egypt Department of Surgery Faculty of Veterinary Medicine Banha University Banha Egypt Department of Medical Physiology Faculty of Medicine Banha University Banha Egypt Department of Medical Biochemistry Faculty of Medicine El Fayoum University Egyptian Egypt

Methylprednisolone（MP） is currently the only drug confirmed to exhibit a neuroprotective effect on acute spinal cord injury（SCI）. Vitamin C（VC） is a natural water-soluble antioxidant that exerts neuroprotective effects through eliminating free radical damage to nerve cells. Bone marrow mesenchymal stem cells（BMMSCs）, as multipotent stem cells, are promising candidates in SCI repair. To evaluate the therapeutic effects of MP, VC and BMMSCs on traumatic SCI, 80 adult male rats were randomly divided into seven groups： control, SCI（SCI induction by weight-drop method）, MP（SCI induction, followed by administration of 30 mg/kg MP via the tail vein, once every other 6 hours, for five times）, VC（SCI induction, followed by intraperitoneal administration of 100 mg/kg VC once a day, for 28 days）, MP ＋ VC（SCI induction, followed by administration of MP and VC as the former）, BMMSCs（SCI induction, followed by injection of 3 × 10~6 BMMSCs at the injury site）, and BMMSCs ＋ VC（SCI induction, followed by BMMSCs injection and VC administration as the former）. Locomotor recovery was assessed using the Basso Mouse Scale. Injured spinal cord tissue was evaluated using hematoxylin-eosin staining and immunohistochemical staining. Expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes was determined using real-time quantitative PCR. BMMSCs intervention better promoted recovery of nerve function of rats with SCI, mitigated nerve cell damage, and decreased expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes than MP and/or VC. More importantly, BMMSCs in combination with VC induced more obvious improvements. These results suggest that VC can enhance the neuroprotective effects of BMMSCs against SCI.

关键词： nerve regeneration spinal cord injury vitamin C methylprednisolone bone marrow mesenchymalstern cells locotmotor neural regeneration

Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients

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World Journal of Gastroenterology 2014年第33期20卷 11793-11799页

作者： Maysa Kamal Salama dina sabry Mohamed AS Al-Ghussein Rasha Ahmed Sayed AbdAllah Fatma Mohamed Taha Wael Fathy Miriam Safwat Wadie Mona Nabih Amr Abul-Fotouh Tarneem Darwish Medical Biochemistry and Molecular Biology Department Faculty of Medicine Cairo University Cairo11562 Egypt Biochemistry Department Faculty of Pharmacy Al-Azhar University Gaza 1277 Palestine Tropical Medicine Department Faculty of Medicine Cairo University Cairo11562 Egypt Internal Medicine Department Faculty of Medicine Cairo University Cairo 11562 Egypt Tropical Medicine Department Faculty of Medicine Bny Swif University Bny Swif 62511 Egypt Biomedical Informatics and Biostatistics Department Faculty of Medicine Cairo University Cairo 11562 Egypt

AIM: To investigate the association of the functional monocyte chemotactic protein-1 (MCP-1) promoter polymorphism (A-2518G) with spontaneous bacterial peritonitis (SBP).

关键词： Monocyte chemotactic protein-1 Genotype Spontaneous bacterial peritonitis Liver cirrhosis As-cites Gene expression Interleukin-10

Low-density lipoprotein receptor genetic polymorphism in chronic hepatitis C virus Egyptian patients affects treatment response

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World Journal of Gastroenterology 2015年第39期21卷 11141-11151页

作者： Mazen Naga Mona Amin dina Algendy Ahmed Elbadry May Fawzi Ayman Foda Serag Esmat dina sabry Laila Rashed Samia Gabal Manal Kamal Division of Gastro-enterology Department of Internal Medicine Faculty of Medicine Cairo University Department of Biochemistry Faculty of Medicine Cairo University Department of Pathology Faculty of Medicine Cairo University Department of Clinical and Chemical pathology Faculty of Medicine Cairo University

AIM: To correlate a genetic polymorphism of the low-density lipoprotein(LDL) receptor with antiviral responses in Egyptian chronic hepatitis C virus(HCV) ***: Our study included 657 HCV-infected patients with genotype 4 who received interferonbased combination therapy. Patients were divided into two groups based on their response to therapy: 356 were responders, and 301 were non-responders. Patients were compared to 160 healthy controls. All patients and controls underwent a thorough physical examination, measurement of body mass index(BMI) and the following laboratory tests: serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total bilirubin, direct bilirubin, prothrombin time, prothrombin concentration, INR, complete blood count, serum creatinine, fasting blood sugar, HCV antibody, and hepatitis B surface antigen. All HCV patients were further subjected to the following laboratory tests: HCV-RNA using quantitative polymerase chain reaction(PCR), antinuclear antibodies, thyroid-stimulating hormone, an LDL receptor(LDLR) genotype study of LDLR exon8 c.1171G>A and exon-10 c.1413G>A using real-time PCR-based assays, abdominal ultrasonography, ultrasonographic-guided liver biopsy, and histopathological examination of liver biopsies. Correlations of LDL receptor polymorphisms with HAI, METAVIR score, presence of steatosis, and BMI were performed in all ***: There were no statistically significant differences in response rates between the different types of interferon used or LDLR exon10 c.1413G>A. However, there was a significant difference in the frequency of the LDL receptor exon8 c.1171G>A genotype between cases(AA: 25.9%, GA: 22.2%, GG: 51.9%) and controls(AA: 3.8%, GA: 53.1% and GG: 43.1%)(P A polymorphism between responders(AA: 3.6%, GA: 15.2%, GG: 81.2%) and nonresponders(AA: 52.2%, GA: 30.6%, GG: 17.

关键词： Hepatitis C virus Genetic polymorphisms Low-densit

Therapeutic Potential of Bone Marrow Derived Mesenchymal Stem Cells in Modulating Astroglyosis of Surgical Induced Experimental Spinal Cord Injury

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Advances in Bioscience and Biotechnology 2016年第6期7卷 251-265页

作者： Moataz A. Elawady Mohammed M. Elmaghrabi Nesrine Ebrahim Mona A. Elawady dina sabry Ashraf Shamaa Alyaa Ragaei Neurosurgery Department Faculty of Medicine Banha University Banha Egypt General Histology Department Faculty of Medicine Banha University Banha Egypt Community Medicine Department Faculty of Medicine Banha University Banha Egypt Medical Biochemistry Department Faculty of Medicine Cairo University Giza Egypt Surgical and Radiology Department Faculty of Veterinary Medicine Cairo University Giza Egypt General Histology Department Faculty of Oral and Dental Medicine Future University Cairo Egypt

Background: Spinal cord injury (SCI) unsuccessful regeneration was due to glial scar development. It was a major obstacle to axonal restoration. Safe therapeutic intervention by the use of bone marrow derived stem cells (BMMSCs) transplantation applied in the present study could reduce spinal disability. Material and methods: Forty male albino rats were divided into four groups: GI: negative control (n = 10 rats);GII: positive control after SCI (n = 10 rats);GIII: SCI + BM - MSCs intravenous injected and GIV: SCI + BM - MSCs intra lesion injected (n = 10 rats in each group). The samples were taken from spinal cord tissues around the region of injury and were subjected to histological, immunohistochemical assessment. RNA extraction and real time PCR for detection of nerve regeneration and astrocyte response to the injury were also performed. Results: Clinical improvement occurred by the enhancement in the Basso, Beattie and Bresnahan (BBB) score after SCI. Histological examinations showed positive regenerative responses in GIV compared to GIII. Conclusion: BM-MSCs transplantation has a promising role in enhancing the microenvironment for nerve regeneration through stumbling the glial scaring formation and inflammatory response after chronic spinal cord injury especially by using intra-lesion route injection.

关键词： Spinal Cord Injury—Bone Marrow Mesenchymal Stem Cells Rats and Glial Tissue

Dendritic cell co-stimulatory and co-inhibitory markers in chronic HCV: An Egyptian study

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World Journal of Gastroenterology 2013年第43期19卷 7711-7718页

作者： Hanan Fouad Maissa Saeed El Raziky Rasha Ahmed Abdel Aziz dina sabry Ghada Mahmoud Abdel Aziz Manal Ewais Ahmed Reda Sayed Medical Biochemistry Department Faculty of MedicineCairo University11562 CairoEgypt Gastroenterology and Tropical Department Faculty of MedicineCairo University11562 CairoEgypt Medical Biochemistry Department Faculty of MedicineBeni Sueif University19206 Kornish Al NileBani SweifEgypt

AIM:To assess co-stimulatory and co-inhibitory markers of dendritic cells(DCs)in hepatitis C virus(HCV)infected subjects with and without ***:Three subject groups were included in the study:group 1 involved 50 control subjects,group2 involved 50 patients with chronic HCV infection and group 3 involved 50 HCV uremic subjects undergoing ***83,CD86 and CD40 as co-stimulatory markers and PD-L1 as a co-inhibitory marker were assessed in peripheral blood mononuclear cells by realtime polymerase chain ***-10(IL-10)and hyaluronic acid(HA)levels were also *** findings were correlated with disease activity,viral load and ***:There was a significant decrease in costimulatory markers;CD83,CD86 and CD40 in groups2 and 3 vs the control ***-stimulatory markers were significantly higher in group 3 vs group *** was a significant elevation in PD-L1 in both HCV groups vs the control ***-L1 was significantly lower in group 3 vs group *** was a significant elevation in IL-10 and HA levels in groups 2 and 3,where IL-10was higher in group 3 and HA was lower in group 3 vs group *** level was significantly correlated with disease activity and fibrosis grade in group ***-10 was significantly correlated with fibrosis grade in group *** were significant negative correlations between co-stimulatory markers and viral load in groups 2 and3,except CD83 in dialysis *** was a significant positive correlation between PD-L1 and viral load in both HCV ***:A significant decrease in DC co-stimulatory markers and a significant increase in a DC coinhibitory marker were observed in HCV subjects and to a lesser extent in dialysis patients.

关键词： Hepatitis C virus Uremia Hemodialysis Dendritic cells CD83 CD86,CD40 PD-L1 Interleukin-10 Hyaluronic acid