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Characterization of a Novel RING-Type Ubiquitin E3 Ligase GhRING2 Differentially Expressed in Cotton Fiber

American Journal of Plant Sciences 2014年第21期5卷 3364-3379页

作者： Shiva Soma Chuan-Yu Hsu Sukumar Saha Johnie N. Jenkins din-pow ma Department of Biochemistry Molecular Biology Entomology and Plant Pa-thology Mississippi State University Mississippi State USA Institute for Genomics Biocomputing & Biotechnology Mississippi State Uni-versity Mississippi State USA Crop Science Research Laboratory Agricultural Research Service United States Department of Agriculture Mississippi State USA Department of Plant and Soil Sciences Mississippi State University Mississippi State USA

The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray analysis, we have identified a novel ubiquitin ligase E3 gene GhRING2 that is differentially expressed between two Gossypium hirsutum lines-Texas marker-1 (TM-1) and Chromosome Substitution Line CS-B25. The CS-B25 line has chromosome 25 from G. barbadense substituted into TM-1. The complete nucleotide sequences of GhRING2 along with its 5’-flanking region were obtained by genomic walking. GhRING2 was highly expressed in elongating fiber, and GUS expression directed by the GhRING2 promoter was found in hypocotyls and young stems of transgenic Arabidopsis plants. Using a yeast two-hybrid assay GhRING2 was found to interact with a PROTODERmaL FACTOR1 (GhPDF1) protein. GhPDF1 was expressed preferentially in immature ovules and fiber initials, and the GhPDF1 gene had been suggested to play a role in cell fate determination and fiber development. Pull down and plasmid swap assays further confirmed the interaction between GhRING2 and GhPDF1. The expression and protein interaction data indicate that GhRING2 may be involved in the turnover of GhPDF1 and participation in the transition from initiation to elongation stages during fiber development. Our data strongly suggest that the ubiquitin-proteasome pathway may regulate cotton fiber growth and development. The nucleotide sequence data of GhRING2 in this article have been submitted to the Gen Bank Nucleotide Sequence Data Bases under the accession number BankIt 1,742,008 SeqKM 108,000.

关键词： Cotton Fiber Gossypium hirsutum G. barbadense RING-Type Ubiquitin Ligase Ubiquitination

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Transcriptome Analysis of Ten-DPA Fiber in an Upland Cotton (<i>Gossypium hirsutum</i>) Line with Improved Fiber Traits from Phytochrome A1 RNAi Plants

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American Journal of Plant Sciences 2017年第10期8卷 2530-2553页

作者： Qing Miao Peng Deng Sukumar Saha Johnie N. Jenkins Chuan-Yu Hsu Ibrokhim Y. Abdurakhmonov Zabardast T. Buriev Alan Pepper din-pow ma Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University Mississippi State MS USA Department of Pharmacology Weill Cornell Medical College New York NY USA USDA-ARS Crop Science Research Laboratory Mississippi State MS USA Institute for Genomics Biocomputing and Biotechnology Mississippi State University Mississippi State MS USA Center of Genomics and Bioinformatics Academy of Sciences of Uzbekistan Tashkent Uzbekistan Department of Biology Texas A & M University College Station TX USA

Silencing phytochrome A1 gene (PHYA1) by RNA interference in Upland cotton (Gossypium hirsutum L. cv. Coker 312) had generated PHYA1 RNAi lines with improved fiber quality (longer, stronger and finer fiber). To reveal molecular mechanisms that govern fiber development with positive fiber traits, a study of global gene expression profiling of 10-DPA fibers in a PHYA1 RNAi line and its parent Coker 312 was conducted by high-throughput RNA sequencing. A comparative analysis of transcriptomes between the two lines had identified 142 genes that were differentially expressed in the 10-DPA fiber of the RNAi line. Gene Ontology analysis showed that these differentially expressed genes were mainly involved in metabolic pathways, heterocyclic/organic cyclic compound binding and multiple enzyme activities, and cell structures which were reported to play important roles in fiber development. Twenty-eight KEGG pathways were mapped for the 142 genes, and the pathways related to glycolysis/gluconeogenesis and pyruvate metabolism were the most abundant and followed by cytochrome P450-involved pathways, suggesting that fiber improvement could be through the regulation of proteins involved in cytochrome P450 pathways. Genes encoding WRKY transcription factors, sucrose synthase, xyloglucan endotransglucosylase hydrolase, udp-glucuronate: xylan alpha-glucuronosyltransferase, and genes involved in lipid metabolism and ABA/brassinosteroid signal transduction pathways were found differentially expressed in the RNAi line. These genes have direct impacts on cotton fiber quality. The results of this study elucidate molecular signatures and possible mechanisms of fiber improvement in the background of PHYA1 RNAi in cotton and should help for future fine-tuning and programming of cotton fiber development.

关键词： Fiber Development Phytochrome A1 RNA Interference RNA-Seq Transcriptome

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Transcriptome Analysis of Ten Days Post Anthesis Elongating Fiber in the Upland Cotton (<i>Gossypium hirsutum</i>) Chromosome Substitution Line CS-B25

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American Journal of Plant Sciences 2018年第6期9卷 1334-1361页

作者： Chuan-Yu Hsu mark A. Arick II Qing Miao Sukumar Saha Johnie N. Jenkins Mirzakamol S. Ayubov Ibrokhim Y. Abdurakhmonov Daniel G. Peterson din-pow ma Institute for Genomics Biocomputing & Biotechnology Mississippi State University Mississippi State MS USA Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University Mississippi State MS USA Department of Pharmacology Weill Cornell Medical College Cornell University New York NY USA USDA-ARS Crop Science Research Laboratory Mississippi State MS USA Center of Genomics and Bioinformatics Academy Sciences of Uzbekistan Tashkent Uzbekistan

A chromosome substitution line, CS-B25, was developed by the substitution of chromosome pair 25 of Gossypium hirsutum TM-1 with the homologous pair of chromosome 25 from G. barbadense, a double haploid Pima 3-79 line. CS-B25 has improved fiber traits compared to its parent TM-1. To explore the molecule mechanisms underlying improved fiber traits, deep sequencing of total RNA was used to compare gene expression in fibers of CS-B25 and TM-1 at 10 days post anthesis (10-DPA). A total of 1872 differentially expressed genes (DEGs) were detected between the two lines, with 1175 up-regulated and 697 down-regulated in CS-B25. Gene Ontology (GO) enrichment analysis of the expression data by Generally Applicable Gene-set Enrichment (GAGE) and ReviGO indicated that the most prevalent Biological Process GO terms associated with DEGs included DNA-templated transcription, response to oxidative stress, and cellulose biosynthesis. Enriched Molecular Function GO terms included structural constituents of cytoskeleton, peroxidase activity, cellulose synthase (UDP-forming) activity, and transcription regulatory region sequence-specific DNA binding factors. GAGE was also used to find enriched KEGG pathways, and the highly represented pathways were Biosynthesis of Amino Acids, Starch and Sucrose Metabolism, Phenylpropanoid Biosynthesis, Protein Processing in Endoplasmic Reticulum, and Plant Hormone Signal Transduction. many of the identified DEGs are involved in cytoskeleton and cell wall metabolism. The results of gene expression data have provided new insight into the molecular mechanisms of fiber development during the fiber elongation stage and would offer novel candidate genes that may be utilized in cotton fiber quality improvement.

关键词： Chromosome Substitution Lines Differentially Expressed Genes Fiber Development Quantitative Real-Time PCR RNA Sequencing Upland Cotton

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Numerical Investigation & Comparison of a Tandem-Bladed Turbocharger Centrifugal Compressor Stage with Conventional Design

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Journal of Thermal Science 2014年第6期23卷 523-534页

作者： Syed Noman Danish Shafiq Rehman Qureshi Abdelrahman EL-Leathy Salah Ud-din Khan Usama Umer ma Chaochen Sustainable Energy Technologies Center King Saud University National University of Sciences and Technology PNS JauharKarachiPakistan Mechanical Engineering Department King Saud University FARCAMT Advanced Manufacturing InstituteKing Saud University Beijing Institute of Technology

Extensive numerical investigations of the performance and flow structure in an unshrouded tandem-bladed centrifugal compressor are presented in comparison to a conventional *** characteristics are explored for various tip clearance levels,axial spacings and circumferential *** impeller was modified to tandem-bladed design with no modifications in backsweep angle,meridional gas passage and camber distributions in order to have a true comparison with conventional *** degradation is observed for both the conventional and tandem designs with increase in tip ***-equation models for correlating stage characteristics with tip clearance are *** two designs,it is clearly evident that the conventional design shows better performance at moderate flow ***;near choke flow,tandem design gives better results primarily because of the increase in throat *** point flow rate also seems to drop for tandem compressor resulting in increased range of operation.

关键词： tandem blade conventional compressor inducer exducer numerical simulation

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ma’s Variation of Parameters Method for Fisher’s Equations

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Advances in Applied mathematics and Mechanics 2010年第3期2卷 379-388页

作者： Syed Tauseef Mohyud-din Ahmet Yıldırım HITEC University Taxila Cantt Pakistan Ege University Department of Mathematics35100 Bornova-IzmirTurkey

In this paper,we apply ma’s variation of parameters method(VPM)for solving Fisher’s *** suggested algorithm proved to be very efficient and finds the solution without any discretization,linearization,perturbation or... 详细信息

关键词： Variation of parameters method variational iteration method nonlinear problems Fisher’s equation nonlinear diffusion equation error estimates

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Tissue distribution of deoxynivalenol in piglets following intravenous administration

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Journal of Integrative Agriculture 2015年第10期14卷 2058-2064页

作者： DENG Xian-bai din Huan-zhong HUANG Xian-hui ma Yong-jiang FAN Xiao-long YAN Hai-kuo LU Pei-cheng LI Wei-cheng ZENG Zhen-ling College of Veterinary Medicine South China Agricultural University

Contamination of deoxynivalenol（DON） in grains is common worldwide and pigs are particularly susceptible to this mycotoxin. The distribution of DON in porcine tissues following intravenous administration was investigated in this study. Fifteen pigs were randomly divided into three groups. Animals in groups A and B were administrated with DON at the dose of 250 and 750 μg kg–1 body weight, respectively, while group C served as blank control. Plasma, bile and 27 tissues were collected at 30 min post-administration. DON concentrations in all samples were tested using high-performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. To observe the distribution of DON in tissues, these samples were further subjected to the immunohistochemical analyses. Totally, the bile and 13 tissues were sampled for DON-based detection, including kidney, mesenteric lymph nodes, muscle, stomach, jejunum, colon, plasma, spleen, rectum, cecum, liver, ileum, and duodenum. No significant difference was observed for the concentrations of DON in duodenum, ileum and liver samples between groups A and B; while the DON concentrations in cecum and rectum of group B were significantly higher（P-value 〈0.05） than those in group A. In addition, the DON concentrations in stomach, jejunum, colon, mesenteric lymph nodes, muscle, kidney, spleen, bile, and plasma of group B were remarkably higher than those of group A（P-value〈0.01）. Levels of DON in other 14 tissues including medulla oblongata, midbrain, diencephalon, pons, tip and tongue body, tongue, soft palate, tonsils, pharyngeal mucosa, oral buccal mucosa, thymus, thyroid, esophagus and adrenal gland were all below the limit of detection. The results of immunohistochemistry showed that 11 tissue samples（medullaoblongata, tonsil, adrenal medulla, thyroid gland, thyroid, stomach, duodenum, jejunum, kidney, spleen, and mesenteric lymph nodes） were positive and DON was mainly distributed around blood vessels in these tissues. Therefore, we be

关键词： deoxynivalenol tissue distribution immunohistochemical staining HPLC-MS/MS swine

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Sensitivity of mountain runoff to climate change for Urumqi and Kaidu rivers originating from the Tianshan Mountains

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Research in Cold and Arid Regions 2011年第3期3卷 274-280页

作者： YongChao Lan ZhengYao ma YongPing Shen ChengFang La Jie Song XingLin Hu HongWei din Key Laboratory of Ecohydrology in Inland River Basin Chinese Academy of Sciences Lanzhou Gansu 730000 China Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou Gansu 730000 China Hydrology and Water Resources Bureau of Gansu Province Lanzhou Gansu 730000 China Hydrology and Water Resources Bureau of the Upper Yellow River Headwater of the Yellow River Conservancy Committee Lanzhou Gansu 730030 China Institute of Hydrogeology and Engineering Geology Gansu Bureau of Geology and Mineral Exploration and Development Zhangye Gansu 734000 China

The mountain watersheds of Kaidu River and Urumqi River, which separately originate from the south and north-side of the Tianshan Mountains in Xinjiang, are selected as the study area. The characteristics and trends on variation of temperature, precipitation and runoff, and the correlativity between temperature, precipitation, and runoffwere analyzed based on the past 40 years of observational data from the correlative hydrological and weather stations in the study areas. Various weather scene combinations are assumed and the response models of runoff to climate change are established in order to evaluate the sensitivity of runoff to climate change in the study areas based on the foregoing analysis, Results show that all variations of temperature, precipitation, and runoff overall present an oscillating and increasing trend since the 1960s and this increase are quite evident after 1990. There is a markedly positive correlation between mountain runoff, temperature, and precipitation while there are obvious regional differences of responding degree to precipitation and temperature between mountain runoff of Ummqi River and Kaidu River Basins Also, mountain runoff of Urumqi River Basin is more sensitive to precipitation change than that of Kaidu River Basin, and mountain runoff of Kaidu River Basin is more sensitive to temperature change than that of Ummqi River Basin.

关键词： south slope north slope Tianshan Mountains Kaidu River Urumqi River climate change sensitivity

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