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Utilization of spent substrates and waste products of mushroom cultivation to produce new crops of Pleurotus ostreatus,Pleurotus eryngii and Agaricus bisporus

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Carbon Resources Conversion 2024年第2期7卷 50-63,F0004页

作者： Marianna Dedousi Eirini-Maria Melanouri dimitris Karayannis Eleftherios-Ikaros Kaminarides Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural Products(ITAP)Hellenic Agricultural OrganizationDimitra(ELGO-Dimitra)1 S.Venizelou str.14123 LykovryssiAttikiGreece Laboratory of Food Microbiology and Biotechnology Department of Food Science and NutritionAgricultural University of Athens75 Iera Odos Str.11855 AthensGreece Green Zin S.A.Agriculture 37008 PaliouriSourpiGreece

Five agro-industrial residues,their corresponding spent mushroom substrates(SMS),commercial fresh and spent mushroom compost of Agaricus(SMC)and Pleurotus ostreatus(SMS GZ)cultivation,Pleurotus and Agaricus waste(stipes/mishappen mushrooms)were combined and re-utilized as novel substrates for the cultivation of ***,*** and *** ***/SMC were used up to 40%(treatment 1 and 2),while 20%of mushroom waste were added in fresh and spent substrates(treatment 3 and 4).The impact of different substrates on mycelial growth rate and biomass production was ***,Pleurotus *** cultivated on the most promising substrates and essential cultivation aspects(earliness,total mushroom yield,biological efficiency-BE)and carposomes’quality parameters(weight,morphological characteristics)were *** and endoglucanase production by Pleurotus species were also determined at 50 and 100%of colonization *** species showed their fastest mycelial growth rate(up to 5 mm/day)on substrates consisted of SMC,whereas many combinations of species/substrate enhanced biomass *** SMS GZ supplementation positively affected laccase activity;in the cultures of *** and *** the highest values were 62,539 and 17,584 U/g d.w.,*** the contrary,small amounts of endoglucanase were produced(0.007 to 0.322 U/g d.w.);the greatest production was recorded for *** at full *** fermentation in bags,significant amount of total mushroom yield was produced in all substrates and those with SMS GZ supported the fastest earliness period and the highest BE for both Pleurotus *** values ranged from 54 to 133%for *** and from 53 to 121%for *** morphological characteristics,mushroom waste addition seemed to affect them *** data included in this paper support the effective re-utilization of different types of SMS and mushroom waste for fungal mass and enzymes’productio

关键词： Oyster mushroom Button mushroom Spent mushroom compost Stipes Misshapen mushrooms Glucosamine Laccase Endoglucanase Earliness Biological efficiency

Recent advances in the production of single cell protein from renewable resources and applications

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Carbon Resources Conversion 2024年第2期7卷 38-49页

作者： Danai Ioanna Koukoumaki Erminta Tsouko Seraphim Papanikolaou Zacharias Ioannou Panagiota Diamantopoulou dimitris Sarris Laboratory of Physico-Chemical and Biotechnological Valorization of Food By-Products Department of Food Science&NutritionSchool of EnvironmentUniversity of the AegeanLeoforos Dimokratias 66Myrina 81400LemnosGreece Department of Food Science and Human Nutrition Agricultural University of Athens75Iera Odos11855 AthensGreece Laboratory of Edible Fungi Institute of Technology of Agricultural ProductsHellenic Agricultural Organization-Dimitra1Sof.Venizelou14123 LykovrysiGreece

Single Cell Protein(SCP)refers to dry cells of microorganisms,and it constitutes a highly promising and alternative protein source for multiple *** presents a rich nutritional profile containing valuable amino acids and fatty acids,nucleic acids,minerals,and several *** businesses worldwide have introduced SCP into their production cycles,hence expanding the scope of its application in value added market chains such as the edible food *** is produced by a plethora of microorganisms,including fungi,yeasts,bacteria,and algae while many of them are Generally Recognized as Safe(GRAS).Selected microbial strains present satisfying growth capability with high yields when cultivated in renewable ***,production rates and process sustainability could be enhanced via the valorization of industrial and agricultural wastes as the nutrient sources,combined with optimization of process parameters,i.e fermentation mode and feeding strategy,pH,temperature,C/N ratio,agitation rate and oxygen *** review addresses the latest developments made towards the SCP production,highlighting efficient microbial SCP producers,and production systems that valorize solid and liquid streams from several agricultural *** applications,challenges in sensorial-,and safety-aspects as well as consumers perception issues of SCP incorporation into food-related matrices are also discussed.

关键词： Alternative protein Sustainability Sensorial aspects Safety Food and feed Packaging applications

Submerged cultivation of selected macro-fungi to produce mycelia rich inβ-glucans and other bioactive compounds,valorizing side streams of the food industry

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Carbon Resources Conversion 2024年第2期7卷 74-87页

作者： Sotirios Pilafidis Erminta Tsouko Glykeria Sougleri Panagiota Diamantopoulou Konstantinos Gkatzionis Zacharias Ioannou dimitris Sarris Laboratory of Physico-Chemical&Biotechnological Valorization of Food By-Products Department of FoodScience&NutritionSchool of the EnvironmentUniversity of the AegeanLeoforos Dimokratias 66Lemnos81400 MyrinaGreece Laboratory of Edible Fungi Institute of Technology of Agricultural ProductsHellenic Agricultural Organization-Dimitra1Sof.Venizelou14123 LykovrysiGreece Laboratory of Consumer and Sensory Perception of Food&Drinks Department of Food Science&NutritionSchool of the EnvironmentUniversity of the AegeanMetropolite Ioakeim 2Lemnos81400 MyrinaGreece

This study reports the valorization of four side-streams derived from the food industry as fermentation media to cultivate edible and medicinal macrofungi of the genera Cyclocybe sp.,Ganoderma sp.,Grifola sp.,Hericium sp.,Morchella sp.,Pleurotus sp.,Schizophyllum *** Trametes sp..Initial screening experiments revealed the suitability of brewer’s spent grain extract(BSGE)and diluted wine distillery effluent(WDE)as the sole carbon sources for significant mycelial mass *** fermentations investigated the effect of static and agitated conditions on biomass production,protein content and glucan content of fungal *** higher biomass and concentrations of total glucans,α-glucans andβ-glucans were determined in macrofungi cultivated in BSGE compared to *** BSGE-based cultures of Schizophyllum commune resulted in the maximum biomass synthesis(27.6 g/L),while the highest total glucans of 70.8%w/w with aβ-glucan content of 57.2%w/w were determined for ***,when the culture was also *** protein content of mycelia ranged from 12.3 up to 26.5%w/w in the strains that were ***-FTIR spectra of the mycelia demonstrated the characteristic bands associated with fungal polysaccharides.

关键词： Biomass Polysaccharides Protein Static Agitated FTIR Bioeconomy Sustainability Edible Medicinal Mushroom Fermentation

Evolution of sex ratio and egg production of gilthead seabream(Sparus aurata)over the course of five reproductive seasons

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Aquaculture and Fisheries 2024年第4期9卷 534-542页

作者： Maria Papadaki dimitris Karamanlidis Eirini Sigelaki Ioannis Fakriadis Constantinos C.Mylonas Institute of Marine Biology Biotechnology and AquacultureHellenic Center for Marine ResearchP.O.Box 2214IraklionCrete71003Greece Department of Biology University of CreteP.O.Box 2208HeraklionCrete71409Greece

Spawning performance-relative fecundity and fertilization success-was studied in two hatchery-reared broodstocks of gilthead seabream(Sparus aurata)held under relatively constant well-water temperature(18-20◦C)and simulated natural photoperiod,for five consecutive spawning seasons,between 3 and 7 years of *** spawning season lasted between 4 and 6 months each year,and the total number of eggs produced annually ranged between 1480000 and 3100000 eggs/kg female body weight,being the highest during the first and second spawning *** difference was observed in monthly relative fecundity between years-although high variation existed within years,whereas fertilization success was the highest during the second and third reproductive season,and dropped significantly during the last year of the *** male percentage of the broodstocks was 35%during the first spawning season of the females,and thereafter it decreased further and remained stable at around 15%-20%for the second and third spawning *** of older females with smaller males to readjust male percentage to 50%in the fourth spawning season,was followed by sex change of larger males to females and a drop of the male percentage to 18%in the following spawning *** present study demonstrated the long spawning season of gilthead seabream-especially under constant water temperature,the high fecundity and fertilization success,and the stabilization of male percentage to~20%after the first spawning season or when a broodstock is modified to increase male *** results are useful to the aquaculture industry,demonstrating that there is no need to add males to gilthead seabream broodstocks over the years,as the sex ratio is stabilized and egg production and fertilization success remain high with a relatively low male:female sex ratio.

关键词： Gilthead seabream Sparus aurata Sex ratio Egg production

Congenital heart“Challenges”in Down syndrome

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World Journal of Cardiology 2024年第5期16卷 217-220页

作者： Maria Drakopoulou Panayotis K Vlachakis Costas Tsioufis dimitris Tousoulis Department of First Cardiology Hippokration General HospitalAthens Medical SchoolNational and Kapodistrian University of AthensAthens 11527Greece

In this editorial,we comment on the article by Kong et al published in the recent issue of the World Journal of *** this interesting case,the authors present the challenges faced in managing a 13-year-old patient with Down syndrome(DS)and congenital heart disease(CHD)associated with pulmonary arterial *** this distinct population,the Authors underscore the need for early diagnosis and management as well as the need of a multidisciplinary approach for decision *** seems that the occurrence of CHD in patients with DS adds layers of complexity to their clinical *** editorial aims to provide a comprehensive overview of the intricate interplay between DS and congenital heart disorders,offering insights into the nuanced diagnostic and therapeutic considerations for physicians.

关键词： Down syndrome Congenital heart disease Atrioventricular septal defect Pulmonary hypertension Right heart catheterization

Pilot study on the effect of flavonoids on arterial stiffness and oxidative stress in chronic kidney disease

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World Journal of Nephrology 2024年第3期13卷 41-48页

作者： Anastasia Vagopoulou Panagiotis Theofilis Despina Karasavvidou Nasra Haddad dimitris Makridis Stergios Tzimikas Rigas Kalaitzidis Nephrology Department“C.Katsinas” General Hospital of Ptolemaida“Mpodosakeio”Ptolemaida 50200Greece Center for Nephrology“G.Papadakis” General Hospital of Nikaia-Piraeus“Agios Panteleimon”Nikaia-Piraeus 18454Greece

BACKGROUND Flavonoids,the main class of polyphenols,exhibit antioxidant and antihypertensive *** To prospectively investigate the impact of flavonoids on arterial stiffness in patients with chronic kidney disease(CKD)stagesⅠ-Ⅳ.METHODS In this prospective,single-arm study,CKD patients with arterial hypertension and diabetes mellitus were *** demographic,clinical,and laboratory variables were *** received daily treatment with a phenol-rich dietary supplement for 3 *** pressure,arterial stiffness(carotidfemoral pulse wave velocity,central pulse pressure),and oxidative stress markers(protein carbonyls,total phenolic compound,total antioxidant capacity)were measured at baseline and at study *** Sixteen patients(mean age:62.5 years,87.5%male)completed the *** intervention,peripheral systolic blood pressure decreased significantly by 14 mmHg(P<0.001).Carotid-femoral pulse wave velocity decreased from 8.9 m/s(baseline)to 8.2 m/s(study end)(P<0.001),and central pulse pressure improved from 59 mmHg to 48 mmHg(P=0.003).Flavonoids also reduced oxidative stress markers including protein carbonyls(P<0.001),total phenolic compound(P=0.001),and total antioxidant capacity(P=0.013).CONCLUSION Flavonoid supplementation in CKD patients shows promise in improving blood pressure,arterial stiffness,and oxidative stress markers.

关键词： Flavonoids Chronic kidney disease Arterial stiffness Oxidative stress Carotid-femoral pulse wave velocity

Stent A pancreaticojejunostomy after pancreatoduodenectomy:Is it always necessary?

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World Journal of Methodology 2024年第3期14卷 23-29页

作者： Dimitrios Symeonidis dimitris Zacharoulis Georgios Tzovaras Labrini Kissa Athina A Samara Eleana Petsa Konstantinos Tepetes Department of Surgery University Hospital of LarissaLarissa 41110Greece Department of General Surgery University Hospital of LarissaLarissa 41110Greece

The establishment of a postoperative pancreatic fistula(POPF)is considered the most common and,concomitantly,the most serious complication associated with pancreaticoduodenectomy(PD).The search for either technical modifications of the operative technique or pharmaceutical interventions that could possibly aid in decreasing the incidence of this often-devastating complication appears *** stenting of the pancreatic duct,with the use of either internal or external stents,has been evaluated in this *** theory,it is an approach that could eliminate many pathophysiological factors responsible for the occurrence of a *** purpose of the present study was to review the current data regarding the role of pancreatic duct stenting on the incidence of POPF,after PD,by using PubMed and Reference Citation *** general,previous studies seem to highlight the superiority of external stents over their internal counterparts in regard to the incidence of POPF;this is at the cost,however,of increased morbidity associated mainly with the stent ***,the use of an internal stent is a less invasive approach with acceptable results and is definitely deprived of the drawbacks arising through the complete diversion of pancreatic juice from the gastrointestinal *** in mind the scarcity of high-quality data on the subject,an approach of reserving stent placement for the high-risk for POPF patients and individualizing the selection between the use of an internal or an external stent according to the distinct characteristics of each individual case scenario appears appropriate.

关键词： Pancreaticoduodenectomy Postoperative pancreatic fistula Pancreatic stent Pancreaticojejunostomy

Improving the operational stability of perovskite solar cells with cesium-doped graphene oxide interlayer

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Journal of Energy Chemistry 2024年第9期96卷 483-490页

作者： Masaud Almalki Katerina Anagnostou Konstantinos Rogdakis Felix T.Eickemeyer Mostafa Othman Minas M.Stylianakis dimitris Tsikritzis Anwar Q.Alanazi Nikolaos Tzoganakis Lukas Pfeifer Rita Therisod Xiaoliang Mo Christian M.Wolff Aïcha Hessler-Wyser Shaik M.Zakeeruddin Hong Zhang Emmanuel Kymakis Michael Grätzel Laboratory of Photonics and Interfaces École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Department of Electrical and Computer Engineering Hellenic Mediterranean UniversityHeraklionCrete 71410Greece Institute of Electrical and Microengineering(IEM)Photovoltaics and Thin-Film Electronics Laboratory(PV-Lab) École Polytechnique Fédérale de Lausanne(EPFL)Neuchâtel 2000Switzerland Department of Nursing Faculty of Health SciencesHellenic Mediterranean UniversityCrete 71410Greece Centre Interdisciplinaire de Microscopie Electronique École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Future Energy Technology Institute King Abdulaziz City for Science and Technology(KACST)P.O.Box 6086Riyadh 11442Saudi Arabia State Key Laboratory of Photovoltaic Science and Technology Shanghai Frontiers Science Research Base of Intelligent Optoelectronics and PerceptionInstitute of OptoelectronicsDepartment of Materials ScienceFudan UniversityShanghai 200433China

Perovskite solar cells(PSCs)have made great advances in terms of power conversion efficiency(PCE),yet their subpar stability continues to hinder their *** interface between the perovskite layer and the charge-carrier transporting layers plays a crucial role in undermining the stability of *** this work,we propose a strategy to stabilize high-performance PSCs with PCE over 23%by introducing a cesium-doped graphene oxide(GO-Cs)as an interlayer between the perovskite and hole-transporting *** GO-Cs treated PSCs exhibit excellent operational stability with a projected T80(the time where the device PCE reduces to 80%of its initial value)of 2143 h of operation at the maximum powering point under one sun illumination.

关键词： Perovskite solar cells Doped graphene oxide Graphene related material Long-term operational stability

Assessing the Economic Value of Vernacular Architecture of Mountain Regions Using Contingent Valuation

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Journal of Mountain Science 2011年第5期8卷 629-640页

作者： Stella GIANNAKOPOULOU dimitris DAMIGOS dimitris KALIAMPAKOS School of Architectural Engineering National Technical University of Athens School of Mining and Metallurgical Engineering National Technical University of Athens

Human pressure on mountain resources poses certain threats to their natural and man-made environment. Although there are many parameters involved, one of the driving forces behind this situation is the emphasis given so far only to the market value of mountain goods and services, ignoring the true social and environmental costs of using mountain resources. Towards this direction, the use of non-market valuation techniques could prove to be beneficial. Bearing in mind the above remarks, the paper presents an application of the Contingent Valuation Method, which aims at estimating the monetary value of a mountain settlement’s vernacular architecture, namely Metsovo. For this purpose, the survey focuses on Metsovo visitors’ willingness to pay a single annual voluntary contribution to an institution that would be founded in order to undertake all necessary actions required to preserve the town’s traditional character. The results are very promising and indicate that vernacular architecture holds a significant economic value that could justify the implementation of appropriate policies towards the protection of vernacular settlements in mountain areas.

关键词： Non-market valuation Vernacular architecture Contingent valuation