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Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

Bio-Design and Manufacturing 2019年第4期2卷 225-241页

作者： Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva Morais Sílvia Vieira Hélder Pereira Joao Espregueira-mendes Rui LReis Joaquim M.Oliveira B′s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 Barco GmrPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimaraesPortugal The Discoveries Centre for Regenerative and Precision Medicine Headquarters at University of MinhoAvepark4805-017 BarcoGuimar'sPortugal Ripoll y De Prado Sports Clinic:Murcia-Madrid FIFA Medical Centre of Excellence MurciaSpain Orthopedic Department Centro Hospitalar Póvoa de Varzim Vila do CondePortugal Clínica do Dragao Espregueira-Mendes Sports CentreFIFA Medical Centre of ExcellencePortoPortugal Dom Henrique Research Centre PortoPortugal Orthopedic Department University of MinhoBragaPortugal

The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patie

关键词： Patient-specific Indirect printing Hierarchical Silk fibroin Enzymatic-cross-linking Meniscus

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Promoter replacement of ANT1 induces anthocyanin accumulation and triggers the shade avoidance response through developmental, physiological and metabolic reprogramming in tomato

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Horticulture Research 2023年第2期10卷 28-40页

作者： João Victor Abreu Cerqueira Feng Zhu Karoline mendes Adriano Nunes-Nesi Samuel Cordeiro Vitor Martins Vagner Benedito Alisdair RFernie Agustin Zsögön Departamento de Biologia Vegetal Universidade Federal de ViçosaViçosa 36570-900 MGBrazil National R&D Center for Citrus Preservation Key Laboratory of Horticultural Plant BiologyMinistry of EducationHuazhong Agricultural University430070 WuhanChina Max-Planck-Institute for Molecular Plant Physiology 14476 PotsdamGermany Division of Plant&Soil Sciences West Virginia UniversityMorgantownWV 26506USA

The accumulation of anthocyanins is a well-known response to abiotic stresses in many plant ***,the effects of anthocyanin accumulation on light absorbance and photosynthesis are ***,we addressed this question using a promoter replacement line of tomato constitutively expressing a MYB transcription factor(ANTHOCYANIN1,ANT1)that leads to anthocyanin ***1-overexpressing plants displayed traits associated with shade avoidance response:thinner leaves,lower seed germination rate,suppressed side branching,increased chlorophyll concentration,and lower photosynthesis rates than the wild ***-rich leaves exhibited higher absorbance of light in the blue and red ends of the spectrum,while higher anthocyanin content in leaves provided photoprotection to high *** of gene expression and primary metabolites content showed that anthocyanin accumulation produces a reconfiguration of transcriptional and metabolic networks that is consistent with,but not identical to those described for the shade avoidance *** results provide novel insights about howanthocyanins accumulation affects the trade-off between photoprotection and growth.

关键词： metabolic expressing programming

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Printability of HDPE/Natural Fiber Composites with High Content of Cellulosic Industrial Waste

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Materials Sciences and Applications 2011年第9期2卷 1331-1339页

作者： Luis Claudio mendes Sibele Piedade Cestari 不详

In this paper, a continuous polymeric matrix highly filled with fiber of sugarcane bagasse has been obtained and its feasibility as an ink-absorbing material has been evaluated. In order to study the effect of the amount of cellulose fiber on the surface printability, contact angle measurement using different liquids—water-based inks, ethanol and ink for ink-jet printers—and printing tests were performed on composites of high density polyethylene (HDPE) and sugarcane bagasse (SCB). The composites were processed in a Haake internal mixer, using the SCB without any previous chemical treatment or compatibilizer. The differential scanning calorimetry (DSC) and derivative thermogravimetry (TG/ DTG) revealed an increase in the thermal stability and in the degree of crystallinity of the HDPE. The optical microscopy (OM) and scanning electron microscopy (SEM) showed that the cellulosic material was homogeneously embedded within the HDPE matrix. In order to assess the resistance of the composite sample to the pull strength of the printer, tensile tests were applied to the composites and the results were compared to known paper samples. The best result was achieved in the composite with the highest content of SCB, as well as the shortest drying time.

关键词： Recycling Composite Printing Properties HDPE Sugarcane Bagasse

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Leukotriene-A4-Hydrolase and Basic Aminopeptidase Activities Are Related with Collagen-Induced Arthritis in a Compartment-Dependent Manner

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Open Journal of Rheumatology and Autoimmune Diseases 2013年第4期3卷 255-262页

作者： Mariana Trivilin mendes Paulo Flavio Silveira Laboratory of Pharmacology Unit of Translational Endocrine Physiology and PharmacologyInstituto ButantanSao PauloBrazil Department of Physiology Instituto de BiociênciasUniversidade de Sao PauloSao PauloBrazil

Objective: Previous study demonstrated the involvement of basic aminopeptidase (APB) activity in the development of collagen-induced arthritis (CIA). Two zinc dependent metalloenzymes (EC 3.***.11.6 and EC 3.***.2.6) are known to exhibit concomitantly APB and leukotriene-A4-hydrolase (LT-A4-H) activities. Influence of the interrelationship between both activities on arthritic processes, however, is presently uncertain. This study aimed to compare these activities in CIA. Methods: CIA was induced in rats and arthritis was assessed macroscopically. Ultracentrifugation was used to separate soluble (S) and solubilized membrane-bound (M) fractions from peripheral blood mononuclear cells (PBMCs) and synovial tissue (ST). Enzyme immunoassay was used to measure LT-A4-H activity, and Real Time Polymerase Chain Reaction was used for evaluating EC 3.***.11.6 and EC 3.***.2.6 gene expressions. Results: The existence of genes for EC 3.***.2.6 and EC 3.***.11.6 was demonstrated in the ST. Compared with control, LT-A4-H activity increased in synovial fluid (SF) and in S-PBMCs of CIA-arthritic and CIA-resistant and in M-ST of CIA-resistant, while it decreased in M-PBMCs of CIA-arthritic and CIA-resistant. In all these locations APB activity remained unchanged or inversely correlated with LT-A4-H activity. Conclusions: LT-A4-H and APB activities in joint-related samples are associated, for the first time, with EC 3.***.2.6 and EC 3.***.11.6 genes, exhibiting a compartment-dependent differential modulation of their specificity, efficiency and/or affinity or an inverse concurrent pattern. Changes in LT-A4-H activity have implications for development or resistance to arthritis in CIA model with a potential to be a diagnostic tool.

关键词： Aminopeptidase Ether Hydrolase Bifunctional Enzyme Eicosanoids

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Size and time dependent internalization of label-free nano-graphene oxide in human macrophages

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Nano Research 2017年第6期10卷 1980-1995页

作者： Rafael G. mendes Angelo Mandarino Britta Koch Anne K. Meyer Alicja Bachmatiuk Cordula Hirsch Thomas Gemming Oliver G. Schmidt Zhongfan Liu Mark H. Rummeli College of Physics Optoelectronics and Energy & Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow University Suzhou 215006 China IFW Dresden Institute for Solid State and Materials Research P.O. Box D-01171 Dresden Germany Centre of Polymer and Carbon Materials Polish Academy of Sciences M. Curie-Sklodowskiej 34 Zabrze 41-819 Poland EMPA - Swiss Federal Laboratories for Materials Science and Technology Lerchenfeldstrasse 5 9014 St. Gallen Switzerland Material Systems for Nanoelectronics Chemnitz University of Technology Reichenhainer Sir. 70 09107 Chemnitz Germany ing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

Graphene oxide shows great promise as a material for biomedical applications, e.g., as a multi-drug delivery platform. With this in view, reports of studies on the interaction between nanosized graphene oxide flakes and biological cells are beginning to emerge. However, the number of studies remains limited, and most used labeled graphene oxide samples to track the material upon endocytosis. Unfortunately, the labeling process alters the surface functionality of the graphene oxide, and this additional funcfionalization has been shown to alter the cellular response. Hence, in this work we used label-free graphene oxide. We carefully tracked the uptake of three different nanoscale graphene oxide flake size distributions using scanning/transmission electron microscopy. Uptake was investigated in undifferentiated human monocyte cells （THP-1） and differentiated macrophage cells. The data show clear size dependence for uptake, such that larger graphene oxide flakes （and clusters） are more easily taken up by the cells compared to smaller flakes. Moreover, uptake is shown to occur very rapidly, within two min of incubation with THP-1 cells. The data highlights a crucial need for cellular incubation studies with nanoparticles, to be conducted for short incubation periods as certain dependencies （e.g., size and concentration） are lost with longer incubation periods.

关键词： graphene oxide THP-1 cells label-free uptake size dependence

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Coping Action Oriented to Early Stimulation of Children with Congenital Zika Virus Infection in Rio de Janeiro State

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Health 2019年第9期11卷 1152-1161页

作者： Miriam ribeiro Calheiros de Sá Paula de Almeida Thomazinho Carla Trevisan Martins ribeiro Natália Anachoreta Molleri Maria Luciana de Siqueira Mayirink Polyanna Nascimento mendes Tatiana Hamanaka Lívia Peluso Rossi Thaís Severino da Silva Dayanne Lucena Maria Elisabeth Lopes Moreira National Institute of Women Children and Adolescents Health Fernandes Figueira-Fiocruz Rio de Janeiro Brazil Rio de Janeiro State Health Department The Superintendent of Primary Health Attention Rio de Janeiro Brazil

New health care demands directed at children with chronic conditions have been demanded mainly after the Zika virus epidemic. Given this, the Brazilian states have produced plans to cope with this congenital infection. In the state of Rio de Janeiro, the elaborated plan had as one of its actions a training proposal for physiotherapists, speech therapists and occupational therapists, focusing on early stimulation and focused on the health care network, especially primary care and specialized rehabilitation centers. Thus the purpose of this article is to describe the impact of a response strategy to Zika virus congenital infection, based on the multi-professional training proposal in earlier stimulation in the public health attention network of Rio de Janeiro State. Descriptive study, observational from the earlier stimulation training course to the public health attention network of Rio de Janeiro State as a coping action to the health care of children with Zika virus congenital infection. At the time of writing, partial results are available after 11 classes were performed. Based on the analysis of the evaluation form of the 11 courses taught by professionals of a referral hospital in Rio de Janeiro and student form, using the Excel program, 299 health network professionals were qualified, from 56 different municipalities of RJ, mostly females (n = 259;86.82%). 175 physiotherapists (59%), 88 speech therapists (29%), 32 occupational therapists (11%) and 4 academics or other professional backgrounds (1%). The articulation between the different levels of public assistance, specifically: primary, secondary and tertiary, in this context assumes a fundamental action for good practices being undertaken considering the population’s health demands. Especially in relation to childhood chronic disease conditions, this action permited the building of lines of care that meet the population’s health demands so that in any of a child’s entry points on the assistance network, eithe

关键词： Delivery of Health Care Zika Virus Chronic Disease Child Intersectoral Collaboration

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In-situ observations of novel single-atom thick 2D tin membranes embedded in graphene

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Nano Research 2021年第3期14卷 747-753页

作者： Xiaoqin Yang Huy Q.Ta Wei Li Rafael G.mendes Yu Liu Qitao Shi Sami Ullah Alicja Bachmatiuk Jinping Luo Lijun Liu Jin-Ho Choi Mark H.Rummeli School of Energy and Power Engineering Xi’an Jiaotong UniversityNo.28Xianning West RoadXian710049China

There is ongoing research in freestanding single-atom thick elemental metal patches,including those suspended in a two-dimensional(2D)material,due to their utility in providing new structural and energetic insight into novel metallic 2D *** pores have shown promise as support systems for suspending such *** study explores the potential of Sn atoms to form freestanding stanene and/or Sn patches in graphene *** atoms were deposited on graphene,where they formed novel single-atom thick 2D planar clusters/patches(or membranes)ranging from 1 to 8 atoms within the graphene *** of three or more atoms adopted either a star-like or close-packed structural *** functional theory(DFT)calculations were conducted to look at the cluster configurations and energetics(without the graphene matrix)and were found to deviate from experimental observations for 2D patches larger than five *** was attributed to interfacial interactions between the graphene pore edges and Sn *** presented findings help advance the development of single-atom thick 2D elemental metal membranes.

关键词： in-situ transmission electron microscopy Sn atoms planar cluster graphene vacancy

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Treatability studies of naphthalene in soil,water and air with persulfate activated by iron(Ⅱ)

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Journal of Environmental Sciences 2020年第4期32卷 67-77页

作者： Gabriela P.mendes Vivian M.A.Magalhaes Lélia C·R·Soares Rayanne M.Aranha Claudio A.O.Nascimento Marilda M.G.R.Vianna Osvaldo Chiavone-Filho Department of Chemical Engineering University of Sao PauloSPf 05508-000Brazil Department of Chemical Engineering Federal University of Rio Grande Do NorteNatalRN59072-970Brazil

Chemical oxidation was applied to an artificially contaminated soil with naphthalene(NAP).Evaluation of NAP distribution and mass reduction in soil,water and air phases was carried out through mass *** of NAP distribution and mass reduction in soil,water and air phases was carried out through mass *** importance of the air phase analysis was emphasized by demonstrating how NAP behaves in a sealed system over a 4 hr reaction *** of Experiments method was applied to the following variables:sodium persulfate concentration[SP],ferrous sulfate concentration[FeS04],and *** system operated with a prefixed solid to liquid ratio of 1:*** following conditions resulted in optimum NAP removal[SP]=18.37 g/L,[FeSO4]=4.25 g/L and pH=*** the end of the 4 hr reaction,62%of NAP was *** the soil phase,the chemical oxidation reduced the NAP concentration thus achieving levels which comply with Brazilian and USA environmental *** the NAP partitioning view,the monitoring of each phase allowed the variabilities assessment over the process,refining the knowledge of mass *** on NAP distribution in the system,this study demonstrates the importance of evaluating the presence of semi-volatile and volatile organic compounds in the air phase during remediation,so that there is greater control of the system as to the distribution and presence of the contaminant in the *** results highlight the importance of treating the contaminant in all its phases at the contaminated site.

关键词： Chemical oxidation Phase distribution Degradation kinetics PAH Soil remediation Mass balance

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Strategies for the analysis of single-tree plot experiments in Eucalyptus plantations

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Journal of Forestry Research 2021年第6期32卷 2437-2445页

作者： Heloisa Guimaraes Santos Flavia Maria Avelar Goncalves Jose Luis Lima Aurelio mendes Aguiar Gabriel Dehon Sampaio Pecanha Rezende Bruno Marco de Lima Magno Antonio Patto Ramalho Departamento de Biologia Setor de Genetica E Melhoramento de PlantasUniversidade Federal de Lavras-UFLALavrasMGBrasil Suzano Papel E Celulose Sao PauloSPBrasil Bracell SP Celulose Ltda Lencóis PaulistaSPBrasil

To verify if the response by Eucalyptus clones in a single-tree plot is influenced by neighboring plants and to determine the effect of missing and/or dominated plants,seven eucalyptus trials in four Brazilian states were analyzed in a randomized complete block design with one plant/plot and 30 *** annual increment(MAI,m^(3)ha^(-1)per year)was determined for three-year-old *** influence of neighboring clones was estimated by a linear regression coefficient between the MAI of each clone/plot and the average of a clonal MAI and its eight *** determine the effect of missing and/or dominated trees,a correction between the MAI and the area available/plant was *** analysis considered unadjusted and adjusted data for missing and dominated *** of accuracy and index of coincidence were used to compare the *** single-tree plots,clone performances were not influenced by neighboring *** accuracy was not increased by any of the adjustments employed and the clone ranking was not altered,and therefore did not justify their use.

关键词： Single-tree plot Missing plant adjustment Eucalyptus breeding

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微型飞片雷管的炸药冲击起爆

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火工情报 2003年第1期 60-65页

作者： R．mendes，J．Campos，I．Plaksin J．ribeiro 徐荩 3030．201Coimbra，Portugal 不详

本文介绍的是用爆炸箔起爆器(EFI)，又称微型飞片雷管，对低密度猛炸药如PETN的冲击爆轰传递(STD)EFI用200nF的电容器形成，电容器可以充电至3．5kV，这个电压可使铜桥爆炸。金属等离子体的膨胀可以使厚度为25岬的Kapton飞片以大约4mm/... 详细信息

本文介绍的是用爆炸箔起爆器(EFI)，又称微型飞片雷管，对低密度猛炸药如PETN的冲击爆轰传递(STD)EFI用200nF的电容器形成，电容器可以充电至3．5kV，这个电压可使铜桥爆炸。金属等离子体的膨胀可以使厚度为25岬的Kapton飞片以大约4mm/μs的速度加速。以作用时间为函数的电流的理论和实验评估与Kapton飞片飞行时间的直接测算有关，为计算飞片系统对炸药的最佳起爆和速度创造了条件。用以与电子条纹高速摄影机连接的64光学纤维带(每根纤维的直径为250μm)为基础的光学方法对9mm长、6．5mm直径的炸药样品的STD过程定了性。所获得的结果，(z，t)曲线图和2ns瞬时分辨率，为爆轰速度，爆轰波(DW)阵面曲线和惰性层中DW产生的冲击波(SW)压力的评估创造了条件。用这种高瞬时空间分辨率方法(high temporal-spatial resolution method)可能鉴别DW传播中的速度脉动。这种结果还显示了飞片速度、起始密度对STD过程和DW速度值的影响。

关键词：微型飞片雷管炸药冲击冲击爆轰传递爆炸箔起爆器

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