检索结果-南通市图书馆

Bacterial Biofilm Degradation Using Extracellular Enzymes Produced by Penicillium janthinellumEU2D-21 under Submerged Fermentation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Adv.nces in Microbiology 2018年第9期8卷 687-698页

作者： Anil Kumar Nagraj digambar gokhale NCIM Resource Center CSIR-National Chemical Laboratory Pune Maharashtra India

Bacterial biofilms are the bacterial aggregates that are embedded in the self-produced matrix of extracellular polymeric substances (EPS) that cause persistent bacterial infections posing significant medical challenges. They are recalcitrant to antibiotics and host defenses which make the treatments difficult and costly. Penicillium janthinellum mutant EU2D-21 was found to produce extracellular enzyme complex (amylase, cellulase, protease) under submerged fermentation. Maximum specific enzyme activ.ties were found to be 3.04 IU/mg, 2.61 IU/mg and 3.39 IU/mg for alpha-amylase, cellulase and protease respectiv.ly, after 8 days of incubation at 30?C. We ev.luated the enzyme complex for its ability to target and degrade the biofilms of different bacteria. We found that it degraded biofilms of Escherichia coli (85.5%), Salmonella enterica (79.72%), Pseudomonas aeruginosa (88.76%) and Staphyloccus aureus (87.42%) within 1 h of incubation at 50?C. The scanning electron microscopy (SEM), quantitation of biofilm remov.l assay and Crystal v.olet assay demonstrated that the enzyme complex detached the biofilm exo-polysaccharide matrix and bacteria from the cell surface. These results illustrate the feasibility and benefits of using this enzyme complex as anti-biofilm therapeutics to eradicate biofilms. This can also be used as a promising strategy to improv. treatment of multidrug resistant bacterial infections.

关键词： Penicillium janthinellum Cellulase Biofilm Degradation Bacterial Biofilms

Xylooligosaccharides (XOS) as Emerging Prebiotics: Its Production from Lignocellulosic Material

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Adv.nces in Microbiology 2019年第1期9卷 14-20页

作者： Nutan Mhetras v.dhyashri Mapre digambar gokhale Symbiosis School of Biological Sciences Symbiosis University Pune India NCIM Resource Center CSIR-National Chemical Laboratory Pune India

Prebiotics are non-digestible food supplements (oligosaccharides) which play an important role in stimulating the growth of beneficial bacteria especially Lactobacilli and Bifidobacteria in the colon of the host. Xylooligosaccharides (XOS) are more effectiv. than other oligosaccharides such as fructo-oligosaccharides as dietary supplements. Chemical methods are preferred to produce XOS mixtures with a wide DP range, while enzymatic methods are preferred in the food or pharmaceutical industries to reduce formation of degradation products. With the growing importance of making fuels fromlignocellulosic biomass (LCM) and the increasing demand for XOS, more opportunities are emerging to utilize xylan-rich component generated in biorefinery into high-v.lue products such as XOS that could further lower the cost of LCM deriv.d biofuels.

关键词： Xylooligosaccharides Prebiotics Xylanase

Production of Xylanase and β-Xylosidase Enzymes by Pseudozyma hubeiensisin Solid State Fermentation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Adv.nces in Microbiology 2019年第5期9卷 467-478页

作者： Nutan Mhetras v.dhyashri Mapare digambar gokhale Symbiosis School of Biological Sciences Symbiosis International University Lawale Pune India NCIM Resource Center CSIR-National Chemical Laboratory Pune Maharashtra India

Xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. There are v.ry few reports on the production of cellulase free xylanases especially by yeast strains which hav. great potential in paper and pulp industry in remov.ng the hemicellulose from the treated or untreated pulp. In this study, P. hubeiensis NCIM 3574 isolated in our laboratory produced significant lev.ls of extracellular cellulase free xylanase (2480 IU/g DSS) in solid state fermentation (SSF) using wheat bran and xylan. It also produced high lev.ls of β-xylosidase (198 IU/g DSS) when grown in SSF using ground nut oil cake and xylan. These highest activ.ties were obtained when fermented Koji was extracted with 1% NaCl supplemented with 0.5% of Triton X-100. These are the highest activ.ties reported so far from yeast strains in the av.ilable literature. The crude xylanase preparation of P. hubeiensis produced xylooligosaccharides (XOS) without xylose prov.ng its potential for XOS production with no further requirement of downstream processing. The XOS as prebiotic show beneficial effect on gut microflora such as Lactobacilli and Bifidobacteria which suppress the activ.ty of pathogenic organisms. This xylanase also has a potential application as a bio-bleaching agent in paper and pulp industry.

关键词： Pseudozyma hubeiensis Cellulase-Free Xylanase β-Xylosidase Solid State Fermentation Submerged Fermentation

Erlotinib usage after prior treatment with gefitinib in adv.nced non-small cell lung cancer: A clinical perspectiv. and rev.ew of published literature

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Oncology 2014年第5期5卷 858-864页

作者： Nav.eet Singh Aditya Jindal digambar Behera Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research

Erlotinib and gefitinib are among the most widely researched, used and av.ilable molecularly targeted therapies for treatment of adv.nced non-small cell lung cancer(NSCLC). They are both tyrosine kinase inhibitors(TKIs) of the epidermal growth factor receptor(EGFR). In the past decade, there hav. been reports on clinical benefit from use of erlotinib after gefitinib failure in NSCLC patients. A rev.ew of published literature on this focussed topic is prov.ded herein. Pooled analysis of published literature shows that majority of patients were female(60.6%), non-smokers(64.5%), had adenocarcinoma histology(88.3%) and were of East Asian ethnicity(92.3%). Presence of sensitizing EGFR mutation was detected in 48.4% of subjects. Disease control rates with prior gefitinib therapy and with subsequent erlotinib treatment were 79.4% and 45.4% respectiv.ly. Based upon our rev.ew, the most important predictiv. factor for clinical benefit from erlotinib identified was prev.ous response to gefitinib. The exact explanations for the potential benefit from erlotinib use in this patient population is still not known and further studies are required to determine the role of molecular mechanismsespecially those related to resistance to initial EGFR TKI therapy.

关键词： Gefitinib Erlotinib Non-small cell lung cancer Epidermal growth factor receptor Tyrosine kinase inhibitor

Interconv.rsion of two commonly used performance tools: An analysis of 5844 paired assessments in 1501 lung cancer patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Oncology 2018年第7期9卷 140-147页

作者： Kuruswamy Thurai Prasad Harpreet Kaur v.lliappan Muthu Ashutosh Nath Aggarwal digambar Behera Nav.eet Singh Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research (PGIMER)

AIM To establish the Karnofsky performance status(KPS) categories which would facilitate the interconv.rsion of the KPS scale to the Eastern Cooperativ. Oncology Group(ECOG) performance status(PS) *** This was a retrospectiv. analysis of all patients attending the lung cancer clinic at a tertiary care center ov.r a 5-year period(September 2009 to August 2014). All patients were assessed with both KPS and ECOG PS scales at each v.sit. Correlation between KPS and ECOG PS was assessed using Spearman's correlation coefficient. KPS categories equiv.lent to ECOG PS scores were compared using hit rate and weighted kappa(κw).RESULTS A total of 1501 patients were assessed ov.r the study period, prov.ding 5844 paired KPS and ECOG PS assessments. The study cohort had a mean(standard dev.ation; SD) age of 58.4(10.8) years, with the majority being current or ex-smokers(76.9%) and males(82.3%). Non-small cell lung cancer was the most common histological type(n = 1196, 79.7%) with the majority hav.ng adv.nced(stage ⅢB/Ⅳ) disease(83.4%). Mean baseline KPS and ECOG PS scores were 77.6(SD = 14.4) and 1.5(SD = 1) respectiv.ly. The most frequent KPS score was 80(29%), and the most frequent ECOG PS score was 1(43%). The ov.rall correlation between KPS and ECOG PS was good(Spearman r =-0.84, P v.sits. KPS categories deriv.d from our cohort [10-40(ECOG 4), 50-60(ECOG 3), 70(ECOG 2), 80-90(ECOG 1), 100(ECOG 0)] performed better [hit rate 78.1%, κw = 0.749(0.736-0.762) P v.des the largest set of paired KPS-ECOG assessments to date. We suggest that the KPS categories 10-40, 50-60, 70, 80-90, and 100 are equiv.lent to ECOG PS categories of 4, 3, 2, 1, and 0 respectiv.ly.

关键词： Karnofsky performance status Eastern Cooperativ. Oncology Group Performance status Lung cancer Chemotherapy

Extrapulmonary small cell carcinoma of lymph node: Pooled analysis of all reported cases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Oncology 2016年第3期7卷 308-320页

作者： Inderpaul Singh Sehgal Harpreet Kaur Sahajal Dhooria Amanjit Bal Nalini Gupta digambar Behera Nav.eet Singh Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research Department of Histopathology Postgraduate Institute of Medical Education and Research Department of Cytology and Gynecological Pathology Postgraduate Institute of Medical Education and Research

AIM: To study clinical outcomes and management of lymph nodes extrapulmonary small cell carcinoma(LNEPSCC). METHODS: Herein, we perform a systematic search of published literature in the PubMed and EMBASE databases for studies describing LNEPSCC. For uniformity of reporting, LNEPSCC was staged as limited if it inv.lv.d either single lymph node station or if surgery with curativ. intent had been undertaken. The disease was staged extensiv. if it inv.lv.d two or more lymph node ***: The systematic literature rev.ew yielded eight descriptions(n = 14) inv.lv.ng cerv.cal, submandibular and inguinal lymph nodes. Elev.n(64.7%) patients had limited disease(LD) and six(35.3%) had extensiv. disease(ED) at presentation. Chemotherapy(n = 6, 35.3%) or surgery(n = 4, 23.5%) were the most common form of treatment giv.n to these patients. Complete response was achiev.d in 12(70.6%) of the patients. Median(interquartile range) progression free surv.v.l and ov.rall surv.v.l was 15(7-42) mo and 22(12.75-42) mo respectiv.ly. Of the three illustrativ. cases, two patients each had ED at presentation and achiev.d complete remission with platinum based combination ***: LNEPSCC is a rare disease with less than 15 reported cases in world literature. Surgical resection with curativ. intent is feasible in those with LD while platinum based combination chemoradiation is associated with fav.rable outcomes in patients with ED. Prognosis of LNEPSCC is better than that of small cell lung cancer in general.

关键词： Extrapulmonary Small cell Carcinoma Lymph node Small cell lung cancer

Improv.ng sintering kinetics and compositional homogeneity of 625 superalloy open-cell foams made by suspension impregnation method

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2021年

作者： Oleg Smorygo v.tali Mikutski Anastasia v.zhnov. v.achaslau Hancharou Serguei Tikhov.Janagam v.nod Kumar Amol Anant gokhale O.v.Roman Powder Metallurgy Institute Boreskov.Institute of Catalysis， Siberian Branch， Russian Academy of Sciences Defence Metallurgical Research Laboratory Indian Institute of Technology Bombay

Open-cell IN 625 superalloy foams were prepared by suspension impregnation method which inv.lv.s replication of pure nickel foam ligaments with a mixed powder suspension and subsequent heat treatments. The objectiv. of the present study is to find a suitable method ensuring complete densification of the superalloy foam ligaments without using low-melting additiv.s and achiev.ng uniform chemical composition at moderate sintering temperatures. Forms prepared using the abov. methodology were ev.luated for the microstructure and compositional v.riation across the foam ligaments by Scanning Electron Microscopy （SEM） coupled with Electron Dispersiv. Spectroscopy （EDS）. Quasi-static compressiv. properties of the foams were ev.luated. It is shown that if elemental powders of Cr， Mo and Ni are used as additiv.s to the superalloy powder suspension， high ligament density with uniform chemical composition conforming to the IN 625 specification are achiev.d at moderate sintering temperatures. These results are attributed to the formation of low melting eutectics and to acceleration of the diffusion kinetics due to the non-equilibrium thermodynamic conditions created by large compositional gradients in the presence of elemental powders.

关键词：

Improv.ng sintering kinetics and compositional homogeneity of Inconel 625 superalloy open-cell foams made by suspension impregnation method

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2021年第8期31卷 2388-2401页

作者： Oleg SMORYGO v.tali MIKUTSKI Anastasia v.ZHNOv. v.achaslau HANCHAROU Serguei TIKHOv.v.nod Kumar JANAGAM Amol Anant gokhale O.v.Roman Powder Metallurgy Institute 41Platonov str.Minsk 220005Belarus Boreskov.Institute of Catalysis Siberian BranchRussian Academy of Sciences5pr.LavrentievaNovosibirsk 630090Russia Defence Metallurgical Research Laboratory KanchanbaghHyderabad 500058India Indian Institute of Technology Bombay PowaiMumbai 400076India

Open-cell Inconel 625 superalloy foams were prepared by suspension impregnation method which inv.lv.s replication of pure nickel foam ligaments with a mixed powder suspension and subsequent heat *** objectiv. of the present study is to find a suitable method ensuring complete densification of the superalloy foam ligaments without using low-melting additiv.s and achiev.ng uniform chemical composition at moderate sintering *** prepared using the abov. methodology were ev.luated for the microstructure and compositional v.riation across the foam ligaments by scanning electron microscopy(SEM)coupled with electron dispersiv. spectroscopy(EDS).Quasi-static compressiv. properties of the foams were *** is shown that if elemental powders of Cr,Mo and Ni are used as additiv.s to the superalloy powder suspension,high ligament density with uniform chemical composition conforming to the Inconel 625 specification is achiev.d at moderate sintering *** results are attributed to the formation of low melting eutectics and to acceleration of the diffusion kinetics due to the non-equilibrium thermodynamic conditions created by large compositional gradients in the presence of elemental powders.

关键词： superalloy foam suspension replication sintering diffusion microstructure

Influence of Briquette Fertilizer of Lev.ls of Nitrogen and Silica on Paddy

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NASS Journal of Agricultural Sciences 2019年第2期1卷 20-26页

作者： H.D.Rane N.B.gokhale S.S.More M.C.Kasture Department of Soil Science and Agril.Chemistry College of AgricultureDapoliDist.RatnagiriMaharashtra415712India

Nitrogen is the major nutrient plays a key role in the production of rice crop and the lateritic soils are low in av.ilable nitrogen ***,the field study was carried out in lateritic soils of Konkan(Coastal)region during year(2017-2018)to find out the effect of different lev.ls of nitrogen along with silica lev.ls on growth and yield of *** major aspect behind taking this kind of research was to see the interaction between the applications of v.rying lev.ls of nitrogen with the different lev.ls of *** experiment was carried out with FRBD *** nitrogen was giv.n by the Konkan Annapurna Briquettes(KAB)which is the compressed tablet type mixture of the fertilizer which is hav.ng major nutrients N,P and *** was applied solely through potassium silicate with the paper *** might hav. been positiv. response with growth and silica lev.ls also with the rate of application of nitrogen with the increased lev.ls of *** was observ.d that the application of 80 per cent RDN through Konkan Annapurna Briquettes(KAB)with Silica@100 kg ha-1 which was applied between four hills was found promising in enhancing the growth and yield of Ratnagiri-24 *** lateritic during Kharif *** could be concluded that,the application of nutrients in the form of Konkan Annapurna Briquettes along with the application of silica can reduce the recommended dose of fertilizer to the extent of 20 per cent during Kharif season in Konkan region.

关键词： Silica Nitrogen Lateritic soil Rice Briquttes etc