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Anticariogenic potentials of clove,tobacco and bitter kola

Asian Pacific Journal of Tropical Medicine 2011年第10期4卷 814-818页

作者： dibua esther uju Nnamani Petra Obioma Department of Microbiology Faculty of Biological SciencesUniversity of NigeriaNsukka 410001Enugu StateNigeria Drug Delivery Research Unit Department of PharmaceuticsFaculty of Pharmaceutical SciencesUniversity of NigeriaNsukka 410001Enugu StateNigeria

Objective:To investigate three tropical plant materials - clove seeds[Syzygium aromalicum(S. aromaticum)],bitter kola fruits[Garcinia kola(***)]and tobacco leaves(Nicotiana species) as potential targeted killers of Streptococcus mutans(***),a cavity-causing bacterium(gram-positive, facultative anaerobe) that resides in a multispecies microbial community(dental plaque) for the treatment of dental caries(tooth decay).Methods:Thirty one(31) teeth samples were collected from patients with obvious signs of tooth decay(swollen gum,weak or fallen tooth,etc.) using sterile swab *** samples were collected from two major denial clinics in Nsukka, Enugu State,Nigeria and investigated by spread inoculation onto sterile blood agar and Mueller Hinton agar(MHA) respectively and incubated at 37℃for 24 *** discrete colonies obtained were further re-inoculated onto sterile Mitis salivarius agar(MSA) plates and incubated as above. The isolates were characterized by gram staining and calalase *** leaves,clove seeds and bitter kola fruits were ground into powder,extracted with three different solvents(n-hexane, hot water and ethanol),filtered,dried and stored in clean containers,corked and kept until used. The plant extracts were investigated for phytochemistry,minimum inhibitory concentration(MIC), minimum cidal concentration(MCC)and compared with some conventional antibiotics commonly used against tooth *** sensitivity test was also carried *** results were statistically ***:The extracts showed varied phytochemical composition but most abundantly the *** result also shows that females(16) have more tooth decay than males(15) and that 16 samples were very bloody while 15 were slightly *** microbial characterization showed that 18 samples were catalase-positive indicating the presence of S. mutans while 13 were catalase-negative suspected to be Staphylococcus *** Gram reaction confirmed 13 Gram-negative and

关键词： Tooth decay Clove Tobacco Bitter kola Extracts Antimicrobial property

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