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Bochdalek hernia (Acute gastric obstruction and laparoscopic approach)

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Health 2011年第10期3卷 609-612页

作者： rafael diaz-Nieto Alvaro Naranjo-Torres 不详

Bochdalek hernia is a rare clinical entity which consist of a lack of development of the diaphragm. Its diagnosis is more common in children and in relation to respiratory symptons. But it is also possible to appear in adulthood and usually related with acute digestive problems. Due to acute presentation often require emergency surgery. After reviewing the current literature, laparoscopic approach seems to be the technique of choice, with or without mesh placement. We report a new case of a young man with acute obstruction syndrome and laparoscopic treatment.

关键词： Cbochdalek Hernia Gastric Obstruction Laparoscopic Approach

Non-redundant Contribution of the Plastidial FAD8 ω-3 Desatumse to Glycerolipid Unsatumtion at Different Temperatures in Arabidopsis

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Molecular Plant 2015年第11期8卷 1599-1611页

作者： Angela Roman Maria L. Hernandez Angel Soria-garcia Sara Lopez-Gomollon Beatriz Lagunas rafael Picorel Jose Manuel Martinez-Rivas Miguel Alfonso Estacion Experimental de Aula Dei (EEAD-CSIC) Avda. Montanana 1005 50059 Zaragoza Spain Instituto de la Grasa (IG-CSIC) Campus Universidad Pablo de Olavide Building 46 Ctra. Utrera kin 1 41013 Seville Spain

Plastidial ω-3 desaturase FAD7 is a major contributor to trienoic fatty acid biosynthesis in the leaves of Arabidopsis plants. However, the precise contribution of the other plastidial ω-3 desaturase, FAD8, is poorly understood. Fatty acid and lipid analysis of several ω-3 desaturase mutants, including two insertion lines of AtFAD7 and AtFAD8, showed that FAD8 partially compensated the disruption of the AtFAD7 gene at 22℃, indicating that FAD8 was active at this growth temperature, contrasting to previous observations that circumscribed the FAD8 activity at low temperatures. Our data revealed that FAD8 had a higher selectivity for 18：2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied. Differences in the mechanism controlling AtFAD7 and AtFAD8 gene expression at different temperatures were also detected. Confocal microscopy and biochemical analysis of FAD8-YFP over-expressing lines confirmed the chloroplast envelope localization of FAD8. Co-localization experiments suggested that FAD8 and FAD7 might be located in close vicinity in the envelope membrane. FAD8-YFP over-expressing lines showed a specific increase in 18：3 fatty acids at 22℃. Together, these results indicate that the function of both plastidial ω-3 desaturases is coordinated in a non-redundant manner.

关键词： Arabidopsis thaliana glycerolipid fatty acid FAD7 FAD8 plastid

Nutraceutical Effect of Cuetlas （Arsenura armida C.） Edible Insects as Local Food at Ixcaquixtla, Mexico

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Journal of Agricultural Science and Technology(A) 2016年第6期6卷 423-428页

作者： Virginia Melo-Ruiz Tomas Quirino-Barreda Susana Macin-Cabrera Karina Sanchez-Herrera rafael diaz-garcia Cesar Gazga-Urioste Department of Biologic Systems Metropolitan Autonomous University Mexico City PC 04960 Mexico Department of Health Care Metropolitan Autonomous University Mexico City PC 04960 Mexico

Insect＇s nutrient composition may provide a good source of macro- and micro-nutrients with nutraceutical functions that could help people from semirural areas, such as Ixcaquixtla, located southeast of the Puebla State, Mexico. The aim of this study was to assess macro- and micro-nutrients in Cuetlas （Arsenura armada C.） larvae and inform local people about the nutraceutical benefits that those insects could provide. Cuetlas larvae samples were collected from Jonote tree on August, 2014 to analyze minerals and macro-nutrient of raw larvae according to AOAC （1995） techniques. Results showed that Cuetlas larvae contained 56.93% proteins, 14.76% lipids, 2.95% minerals, 2.13% fiber and 23.23% soluble carbohydrates. Cuetlas larvae have a good amount of proteins, essential macromolecules for human life as well as essential fatty acids that are important sources of fuel for brain cells and in particular for cardiac and skeletal muscle, minerals, not determined individually, which are important for metabolism processes, fiber for digestive processes and soluble carbohydrates as energy source.

关键词： Cuetlas edible insects nutrition health nutraceutical.

Operational definitions and measurement of externalizing behavior problems:An integrative review including research models and clinical diagnostic systems

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World Journal of Psychiatry 2023年第6期13卷 278-297页

作者： Lidia Torres-Rosado Oscar M Lozano Manuel Sanchez-garcia Fermín Fernández-Calderón Carmen diaz-Batanero Department of Clinical and Experimental Psychology University of HuelvaHuelva 21071Spain Research Center for Natural Resources Health and EnvironmentUniversity of HuelvaHuelva 21071Spain

Measurement of externalizing disorders such as antisocial disorders,attentiondeficit/hyperactivity disorder or borderline disorder have relevant implications for the daily lives of people with these *** the Diagnostic and Statistical Manual of Mental Disorders(DSM)and the International Classification of Diseases(ICD)have provided the diagnostic framework for decades,recent dimensional frameworks question the categorical approach of psychopathology,inherent in traditional *** and instruments develop under the DSM or ICD framework preferentially adopt this categorical approach,providing diagnostic *** contrast,dimensional measurement instruments provide an individualized profile for the domains that comprise the externalizing spectrum,but are less widely used in *** paper aims to review the operational definitions of externalizing disorders defined under these different frameworks,revise the different measurement alternatives existing,and provide an integrative operational ***,an analysis of the operational definition of externalizing disorders among the DSM/ICD diagnostic systems and the recent Hierarchical Taxonomy of Psychopathology(HiTOP)model is carried ***,in order to analyze the coverage of operational definitions found,a description of measurement instruments among each conceptualization is *** phases in the development of the ICD and DSM diagnosis systems can be observed with direct implications for *** and DSM versions have progressively introduced systematicity,providing more detailed descriptions of diagnostic criteria and categories that ease the measurement instrument ***,it is questioned whether the DSM/ICD systems adequately modelize externalizing disorders,and therefore their *** recent theoretical approaches,such as the HiTOP model seek to overcome some of the criticism raised towards the classification ***,several issues

关键词： Externalizing disorders Measurement Diagnostic and Statistical Manual of Mental Disorders International Classification of Diseases Hierarchical Taxonomy of Psychopathology Psychopathology

Paclitaxel-loaded hollow-poly（4-vinylpyridine） nano- particles enhance drug chemotherapeutic efficacy in lung and breast cancer cell lines

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Nano Research 2017年第3期10卷 856-875页

作者： rafael Contreras-Caceres Maria C. Leiva Raul Ortiz Amelia diaz Gloria Perazzoli Miguel A.Casado-Rodriguez Consolacion Melguizo Jose M. Baeyens Juan M. Lopez-Romero Jose Prados Department of Organic Chemistry Faculty of Science University of Mdlaga 29071 Malaga Spain Institute of Biopathology and Regenerative Medicine (IBIMER) Center of Biomedical Research (CIBM) University of Granada 18100 Granada Spain Department of Anatomy and Embryology Faculty of Medicine University of Granada 18071 Granada Spain Biosanitary Institute of Granada (IBS. GRANADA) SAS-Universidad de Granada 18014 Granada Spain Department of Health Science University of Jaen 23071 Jadn Spain Department of Pharmacology Institute of Neuroscience Biomedical Research Center (CIBM) University of Granada 18100 Granada Spain

Paclitaxel （PTX）, one of the most effective cytotoxins for the treatment of breast and lung cancer, is limited by its severe side effects and low tumor selectivity. In this work hollow-poly（4-vinylpyridine）（hollow-p4VP） nanoparticles （NPs） have been used for the first time to generate PTX@p4VP NPs, employing a novel technique in which a gold core in the center of the NP is further oxidized to produce the hollow structure into which PTX molecules can be incorporated. The hollow-p4VP NPs exhibit good physicochemical properties and displayed excellent biocompatibility when tested on blood （no hemolysis） and cell cultures （no cytotoxicity）. Interestingly, PTX@p4VP NPs significantly increased PTX cytotoxicity in human lung （A-549） and breast （MCF-7） cancer cells with a significant reduction of PTX ICs0 （from 5.9 to 3.6 nM in A-549 and from 13.75 to 4.71 nM in MCF-7）. In addition, PTX@p4VP caused a decrease in volume of A-549 and MCF-7 multicellular tumor spheroids （MTS）, an in vitro system that mimics in vivo tumors, in comparison to free PTX. This increased antitumoral activity is accompanied by efficient cell internalization and increased apoptosis, especially in lung cancer MTS. Our results offer the first evidence that hollow- p4VP NPs can improve the antitumoral activity of PTX. This system can be used as a new nanoplatform to overcome the limitations of current breast and lung cancer treatments.

关键词： paclitaxel poly(4-vinylpyridine)(p4VP) nanoparticles lung cancer breast cancer cytotoxicity multicellular tumorspheroids

负载型钌催化剂催化山梨醇氢解制乙二醇(英)

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催化学报 2014年第5期35卷 614-621页

作者： Inmaculada Murillo Leo Manuel Lopez Granados Jose Luis garcia Fierro rafael Mariscal Group of Sustainable Energy and Chemistry Institute of Catalysis and Petrochemistry(CSIC)

Supported Ru catalysts were prepared by wet impregnation to evaluate the role of different oxide supports(Al_2O_3,SiO_2,TiO_2,ZrO_2) in sorbitol hydrogenolysis to glycols.X-ray diffraction,transmission electron microscopy,hydrogen chemisorption,X-ray photoelectron spectroscopy,and NH3temperature-programmed desorption were used to characterize the catalysts,which were active in the hydrogenolysis of *** support affected both the physicochemical properties and catalytic behavior of the supported Ru *** characterization results revealed that the Ru/Al_2O_3catalyst has a high surface acidity,partially oxidized Ru species on the surface,and a higher surface Ru/Al atomic ratio,which gave it the highest selectivity and yield to glycols.

关键词：负载钌催化剂山梨醇过负载二元醇 Ru Al2O3催化剂氢解 X射线光电子能谱透射电子显微镜

Portal hypertensive colopathy is associated with portal hypertension severity in cirrhotic patients

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World Journal of Gastroenterology 2009年第38期15卷 4781-4787页

作者： Antonio diaz-Sanchez Oscar Nuez-Martinez Cecilia Gonzalez-Asanza Ana Matilla Beatriz Merino Diego Rincon Inmaculada Beceiro Maria Vega Catalina Magdalena Salcedo rafael Baares Gerardo Clemente Liver Unit-CIBEREHD.Hospital General Universitario Gregorio Maraón Madrid 28007Spain Gastroenterology Unit Hospital Infanta Leonor Gastroenterology Unit Hospital Infanta Sofia Department of Gastroenterology Hospital Universitario Príncipe de Asturias

AIM： To assess the prevalence of portal hypertension （PH） related colorectal lesions in liver transplant candidates, and to evaluate its association with the severity of PH. METHODS： Between October 2004 and December 2005, colonoscopy was performed in 92 cirrhotic liver transplant candidates. We described the lesions resulting from colorectal PH and their association with the grade of PH in 77 patients who underwent measurement of hepatic venous pressure gradient （HVPG）. RESULTS： Mean age was 55 years and 80.7% of patients were men. The main etiology of cirrhosis wasalcoholism （45.5%）. Portal hypertensive colopathy （PHC） was found in 23.9%, colonic varices in 7.6% and polyps in 38% of patients （adenomatous type 65.2%）. One asymptomatic patient had a well-differentiated adenocarcinoma. The manifestations of colorectal PH were not associated with the etiology of liver disease or with the Child-Pugh grade. Ninety percent of patients with colopathy presented with gastroesophageal varices （GEV）, and 27.5% of patients with GEV presented with colopathy （P = 0.12）. A relationship between higher values of HVPG and presence of colopathy was observed （19.9：1：6.2 mmHg vs 16.8 ± 5.4 mmHg, P = 0.045）, but not with the grade of colopathy （P = 0.13）. Preneoplastic polyps and neoplasm （P = 0.02） and spontaneous bacterial peritonitis （P = 0.006） were more prevalent in patients with colopathy. We did not observe any association between previous β-blocker therapy and the presence of colorectal portal hypertensive vasculopathy. CONCLUSION： PHC is common in cirrhotic liver transplant candidates and is associated with higher portal pressure.

关键词： Liver cirrhosis Portal hypertension Polyps Colopathy Liver transplantation

The use of high resolution anterior segment optical coherence tomography for the characterization of conjunctival lymphoma, conjunctival amyloidosis and benign reactive lymphoid hyperplasia

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Eye and Vision 2019年第1期6卷 123-130页

作者： Nandini Venkateswaran Carolina Mercado Ann Q.Tran Armando garcia Pedro Francisco Monsalve diaz Sander R.Dubovy Anat Galor Carol L.Karp Bascom Palmer Eye Institute University of Miami Miller School of Medicine900 NW 17th StreetMiamiFL 33136USA Department of Ophthalmology Miami Veterans Administration Medical CenterMiamiFLUSA

Background:Conjunctival lymphoma,conjunctival amyloidosis and benign reactive lymphoid hyperplasia(BRLH)are conditions that often have a similar appearance on the ocular *** use of high resolution anterior segment optical coherence tomography(HR-OCT)enables clinicians to evaluate distinctive differences in tissue morphology and cellular patterns in various ocular surface *** this study,we characterize the morphological differences seen in conjunctival lymphoma,conjunctival amyloidosis and BRLH on HR-OCT ***:A retrospective chart review was performed of patients with biopsy proven conjunctival lymphoma,conjunctival amyloidosis and BRLH between 2012 and 2019 at the Bascom Palmer Eye *** were excluded if HR-OCT imaging was not performed on initial ***:Thirty-four total eyes of 27 patients were *** eyes had conjunctival lymphoma(16 patients),8 eyes had conjunctival amyloidosis(6 patients)and 6 eyes had BRLH(5 patients).All conditions appeared clinically as pink,red or yellow subepithelial lesions but had different features on *** lymphoma,HR-OCT images typically showed homogenous,dark subepithelial lesions with smooth borders,containing monomorphic dot-like ***-OCT images of amyloidosis typically showed heterogeneous,dark lesions with irregular borders,often containing hyperreflective linear ***-OCT images of BRLH showed variable infiltration of the subepithelial tissue,at times with homogenous lesions containing dot-like infiltrates like lymphoma and other times with more hyperreflective,subepithelial *** cytometry and gene rearrangement was needed for final differentiation between BRLH and lymphoma ***:Distinctive features on HR-OCT of conjunctival lymphoma,conjunctival amyloidosis and BRLH can help characterize these lesions beyond what is apparent with the clinical *** studies can further validate this technology’s use with m

关键词： High resolution anterior segment optical coherence tomography Ocular surface imaging Ocular surface lesions Conjunctival lymphoma Conjunctival amyloidosis Benign reactive lymphoid hyperplasia

Acute hepatitis with autoimmune features after COVID-19 vaccine:coincidence or vaccine-induced phenomenon?

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Gastroenterology Report 2022年第1期10卷 628-631页

作者： Jose M.Pinazo-Bandera Alicia Hernandez-Albujar Ana Isabel garcia-Salguero Isabel Arranz-Salas Raul J.Andrade Mercedes Robles-diaz Division of Gastroenterology and Hepatology Instituto de Investigacion Biomedica de Malaga-IBIMAHospital Universitario Virgen de la VictoriaUniversidad de MalagaMalagaSpain Division of Anatomical Pathology Hospital Universitario Virgen de la VictoriaMalagaSpain Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas(CIBERehd) MadridSpain

Introduction Autoimmune diseases result from a breach of immunological self-tolerance and tissue damage by autoreactive T *** acute respiratory syndrome coronavirus-2(SARS-CoV-2)infection is characterized by an inflam... 详细信息

关键词： autoimmune vaccine acute