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Open Journal of Optimization 2023年第2期12卷 11-24页

作者： Jean-Kisito Kouamé Mariette Désirée Yéhé Claude Bérenger Ngalemo Ngantchouko Amani Germain Brou Carine Nina Ablé Aya Mélissa diane Kouadio Bi Gogoua Désiré Vagny Vincent De Paul Ovi Christophe N’cho Amin Gildas Komenan Gbassi National Public Health laboratory (NPHl) Food Control Service Abidjan Ivory Coast Department of Analytical Sciences and Public Health Training and Research Unit (UFR) of Pharmaceutical and Biological Sciences Félix Houphou&#235t Boigny University Abidjan Ivory Coast Higher Institute of Health Sciences of the University of the Mountains (UdM) Bangangté Cameroon

In order to provide the population with safe, effective and good quality medicines, the pharmaceutical industries, before releasing batches of their products into the pharmaceutical circuit, put in place internal dosage methods to control the quality of these products. The present study consisted in optimizing a method for the simultaneous determination of Phloroglucinol (PHG) and Trimethylphloroglucinol (TPH) by high performance liquid chromatography (HPlC) routinely used in a pharmaceutical industry located in a township in Abidjan (Ivory Coast). The basic chromatographic conditions were those routinely used for the determination of these two molecules: mobile phase: acetonitrile/water (60/40), stationary phase (C18 BDS Hypersil 250 mm * 4.6 mm - 5 μm), detection wavelength (265 nm), flow rate, injection volume and run time configured at the equipment level were respectively 1 ml/min, 10 μl and 8 min. The method of preparation of the analytes (PHG and TPHG) was also applied by the pharmaceutical industry. The application of these different parameters at the equipment level made it possible to determine a chromatogram which highlights three chromatographic peaks with respective retention times (RT) of 0.773 min (unidentified compound), 2.275 min (PHG) and 7.269 min for an analysis time of 8 min with a better resolution of the peaks and baseline. The progressive optimization of different parameters such as the stationary phase (C18 YMC 150 mm * 4.6 mm - 3 μm), the proportion of the mobile phase: acetonitrile/water (80/20), the flow rate impelled by the pump (0.8 ml/min) and the modification of the analyte preparation mode (same amount of PHG and TPHG in a 50 ml volumetric flask) resulted in a final chromatogram that highlighted two chromatographic peaks at the respective RT of 2.391 min (PHG) and 3.735 min (TPHG) at a run time of 6 min. The chromatographic conditions that led to the final chromatogram can be used routinely by the pharmaceutical industry for the dete

关键词： PHG TPHG Pharmaceutical Industry Chromatographic Profile Chromatographic Peak

*** rigidity is correlated with glaucomatous structural damage

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Annals of Eye Science 2019年第1期 201-201页

作者： diane N.Sayah Javier Mazzaferri Denise Descovich Santiago Costantino Mark R.lesk Maisonneuve-Rosemont Hospital Research Center MontrealQCCanada Department of Ophthalmology Faculty of MedicineUniversitéde MontréalMontrealQCCanada Centre Universitaire d'ophtalmologie de l'Universitéde Montréal de l'Hôpital Maisonneuve-Rosemont CIUSSS-EMontrealQCCanada

Background:The rigidity of the corneoscleral shell is an important biomechanical property which could be relevant in the pathophysiology of open-angle glaucoma(OAG).This study aims to evaluate the relationship between ocular rigidity(OR)and glaucomatous damage as represented by structural optical coherence tomography(OCT)-based parameters such as retinal nerve fiber layer(RNFl)and ganglion cell layer+inner plexiform layer(GCl+IPl)*** parameters characterize the retinal layers that contain neuronal structures that form the optic ***:Sixty-six subjects(37 with early OAG,11 with moderate to advanced OAG,16 healthy)were recruited in this *** measurements were carried out using a non-invasive clinical method developed by our *** described in Beaton et al.(2015),this method,which is based on Friedenwald’s equation,involves video-rate OCT imaging and automated choroidal segmentation,as well as dynamic contour tonometry to calculate the OR *** and macular GCl thicknesses were acquired using the Cirrus SD-OCT(Carl-Zeiss Meditec,Dublin,CA,USA).Correlations between OR and structural parameters in all 66 eyes were assessed using ***:Significant correlations were found between OR and the average GCl+IPl thickness(r=0.355,P=0.004)as well as the minimum GCl+IPl thickness(r=0.340,P=0.006).Direct correlations were also found between OR and RNFl thickness in the inferior quadrant(r=0.258,P=0.036)and inferior clock hour(r=0.313,P=0.011).Conclusions:In this study,we found a positive correlation between structural OCT-based parameters and OR,perhaps indicating more structural damage in less rigid *** findings could provide insight unto the pathophysiology of *** investigation is warranted to confirm the role of OR in glaucoma and elucidate whether there is a subgroup of patients for which OR plays a greater role.

关键词： Ocular rigidity(OR) retinal nerve fiber layer(RNFl) ganglion cell complex open-angle glaucoma(OAG)

子宫颈癌筛查异常妇女处理的共识指南(2006版)

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循证医学 2008年第4期8卷 239-247页

作者：李志刚（翻译） Thomas C. Wright Jr l Stewart Massad Charles J. Dunton MarkSpitzer Edward J. Wilkinson dianeSolomon 广东省人民医院妇产科广州510080 the 2006 American Society forColposcopy and Cervical Pathology-Sponsored ConsensusConference

2001年，美国阴道镜与宫颈病理学会（American Society for Colposcopyand Cervical Pathology，ASCCP）联合其他专业学会以及联邦与国际组织．制订了2001版的宫颈癌细胞学筛查结果异常妇女处理的共识指南。这是全面的、以循证医学为依... 详细信息

2001年，美国阴道镜与宫颈病理学会（American Society for Colposcopyand Cervical Pathology，ASCCP）联合其他专业学会以及联邦与国际组织．制订了2001版的宫颈癌细胞学筛查结果异常妇女处理的共识指南。这是全面的、以循证医学为依据的指南，其目的是帮助所有亚专业的临床医生，处理每年约480万诊断为细胞学异常的美国妇女。

关键词：宫颈癌筛查意义未明的不典型鳞状细胞低度鳞状上皮内病变高度鳞状上皮内病变宫颈细胞学人乳头状瘤病毒测定临床指引

AB036. Pulsatile choroidal blood flow (PCBF) in the glaucoma spectrum-preliminary results obtained with a novel optical method

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Annals of Eye Science 2018年第1期 442-442页

作者： Wenzhen Zuo diane N.Sayah Javier Mazzaferri Santiago Costantino Mark R.lesk Centre de recherche de l'hôpital Maisonneuve-Rosemont MontréalQCCanada Facultéde Médecine Universitéde MontréalMontrealQCCanada Department of Ophthalmology Universitéde MontréalMontrealQCCanada

Background:Decrease of ocular blood flow has been linked to the pathogenesis of ocular diseases such as glaucoma and age-related macular *** methods that measure the pulsatile blood flow have major limitations,including the assumption that ocular rigidity is the same in all *** group has recently developed a new method to measure the pulsatile choroidal volume change by direct visualization of the choroid with OCT imaging and automated *** goal in this study is to describe the distribution of PCBF in a healthy Caucasian ***:Fifty-one subjects were recruited from the Maisonneuve-Rosemont Hospital Ophthalmology Clinic and underwent PCBF measurement in one *** distribution of PCBF in healthy eyes was ***:The distribution of PCBF among the healthy eyes was found to be 3.94±1.70µl with this ***:This study demonstrates the normal range of PCBF values obtained in a healthy Caucasian *** technique could be used for further investigation of choroid pulsatility and to study glaucoma pathophysiology.

关键词： Pulsatile choroid blood flow(PCBF) glaucoma pathophysiology novel method

AB091.A novel method for the measurement of pulsatile choroidal blood flow based on video-rate OCT imaging and automated segmentation of the choroid:description and reproducibility

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Annals of Eye Science 2018年第1期 497-497页

作者： diane N.Sayah Wenzhen Zuo Javier Mazzaferri luke Beaton Santiago Costantino Mark R.lesk Centre de recherche de l'Hôpital Maisonneuve-Rosemont MontrealQCCanada Département d'Ophtalmologie Universitéde MontréalMontrealQCCanada Facultéde Médecine Universitéde MontréalMontrealQCCanada

Background:Over the years,a variety of non-invasive techniques have been developed to allow the measurement of blood flow in living human ***,none of the existing techniques has yet been adopted in clinical practice due to their limitations and lack of ***,no reliable technique is currently available to measure the pulsatile choroidal blood flow(PCBF).We propose a novel method based on video-rate optical coherence tomography(OCT)imaging and automated segmentation to measure the pulsatile component of choroidal blood flow in vivo,and demonstrate its ***:Adapted from our earlier work(Beaton et al.),this method uses video-rate OCT with enhanced depth imaging and automated segmentation of the choroid to measure the pulsatile choroidal volume *** is carried out at the fundus for less than a minute at 7 *** each frame,choroidal thickness(CT)is measured by a segmentation algorithm based on graph cuts using an edge-probability weighting *** algorithm computes the CT change corresponding to choroidal filling over the time-series and subsequently derives the pulsatile choroidal volume change through an approximate model of the ***-eight subjects were recruited from the Maisonneuve-Rosemont Hospital and PCBF was measured twice in one eye within the same session and by a single *** was assessed using the Bland-Altman plot and Intraclass correlation coefficient as calculated with ***:Two measurements of PCBF were successfully obtained for each eye using our *** average measures ICC for choroidal volume change was 0.929(95%CI,0.881,0.958),showing good to excellent *** Bland-Altman plot and Pearson coefficient(r=0.840,Plation respectively between intra-session measurement of OR in all examined ***:This study confirms the high repeatability of pulsatile choroidal blood flow measurements obtained with our

关键词： Pulsatile choroidal blood flow(PCBF) optical coherence tomography(OCT) novel technique repeatability

空气污染暴露与妊娠期糖耐量异常:Viva队列项目

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环境与职业医学 2014年第7期31卷 533-533页

作者： Abby F.Fleisch diane R.Gold Sheryl l.Rifas-Shiman Petros Koutrakis Joel D.Schwartz Itai Kloog Steven Melly Brent A.Coull Antonella Zanobetti Matthew W.Gillman Emily Oken

[背景]已知细颗粒物（PM直径≤2．5μm，PM2.5）暴露与2型糖尿病相关，但是PM2.5与妊娠期高血糖的相关性尚未进行充分的研究。[方法]对马萨诸塞州波士顿地区没有糖尿病史的孕妇进行研究。在妊娠期中位数28．1周时，通过临床葡萄糖耐量... 详细信息

[背景]已知细颗粒物（PM直径≤2．5μm，PM2.5）暴露与2型糖尿病相关，但是PM2.5与妊娠期高血糖的相关性尚未进行充分的研究。[方法]对马萨诸塞州波士顿地区没有糖尿病史的孕妇进行研究。在妊娠期中位数28．1周时，通过临床葡萄糖耐量试验，确定妊娠期糖耐量减低（IGT）和妊娠期糖尿病（GDM）。通过中央监测地点和时空模型，使用住宅地址估计妊娠中期的PM2.5和炭黑暴露。估计住宅区交通密度和道路距离，作为交通源空气污染暴露的替代指标。进行多因素logistic回归分析，以社会人Iz／学协变量进行调整，并采用多重填补法解释丢失的数据。[结果]2093名妇女中，65名（3％）存在IGT，118名（6％）患有GDM。妊娠中期时空暴露范围为：PM2.5，8．5～15．9μm／m^3；炭黑，0．1-1．7μm／m^3。在100m范围内，交通密度为0—30860辆／日×道路长度（km）；281名（13％）妇女住宅距离主要道路≤200m。与最低四分位数相比，暴露于时空PM2.5和交通密度的最高四分位数的IGT患病率升高，分别为比值比（OR）=2．63，95％CI：1．15～6．01以及OR=2．66，95％CI：1．24—5．71。IGT也与其他暴露指标呈正相关，尽管这些关联没有统计学意义。无污染物暴露与GDM呈正相关。[结论]妊娠期PM2.5和其他交通源污染物暴露越高与IGT相关，与GDM不相关。空气污染可能导致妊娠期血糖异常。

关键词：妊娠期糖耐量异常空气污染妊娠期糖耐量减低葡萄糖耐量试验队列妊娠期糖尿病 2型糖尿病 PM2 5

儿童嗜酸性粒细胞性食管炎患者牛乳蛋白特异性IgG4抗体检测

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中华临床免疫和变态反应杂志 2018年第4期12卷 459-473页

作者： Alexander J. Schuyler Jeffrey M. Wilson Anubha Tripathi Scott P. Commins Princess U. Ogbogu Patrice G. Kruzsewski Barrett H. Barnes Emily C. McGowan lisa J. Workman Jonas lidholm Sheryl l. Rifas-Shiman Emily Oken diane R. Gold Thomas A. E. Platts-Mills Elizabeth A. Erwin 陈轩馥(翻译) 杨红(审校) 不详中国医学科学院北京协和医学院北京协和医院消化内科

背景变应原特异性IgG4 (sIgG4)抗体通常与耐受性相关,目前已有报道称在成人嗜酸性粒细胞性食管炎(EoE)患者中,sIgG4抗体与某些食物有关,但目前尚缺乏儿科患者针对某些食物的sIgG4抗体阳性率及滴度的研究。目的调查EoE患儿和未经选择的... 详细信息

背景变应原特异性IgG4 (sIgG4)抗体通常与耐受性相关,目前已有报道称在成人嗜酸性粒细胞性食管炎(EoE)患者中,sIgG4抗体与某些食物有关,但目前尚缺乏儿科患者针对某些食物的sIgG4抗体阳性率及滴度的研究。目的调查EoE患儿和未经选择的对照幼儿中抗某些食物的血清sIgG4水平,并探讨性别、年龄和牛奶摄入量对sIgG4水平的影响。方法对71例EoE患儿和210名青春期儿童(源自未经选择的出生队列,此队列来自Viva项目)的血清进行检测,测定抗牛乳(cow's milk,CM)中主要蛋白(α乳清蛋白、β乳球蛋白、酪蛋白)、小麦、大豆、鸡蛋、花生蛋白的sIgG4和特异性IgE (specific IgE,sIgE)水平。结果与对照组相比,EoE患儿血清中抗牛乳蛋白sIgG4滴度(≥10μg/ml)普遍升高,两组的比值比为5. 5～8. 4,而EoE患儿抗牛乳蛋白sIgE水平多≤4 U/ml,因此sIgG4/sIgE的比值通常≥10 000。当对年龄和牛奶摄入量进行调整后发现,高滴度的抗牛乳蛋白sIgG4水平与EoE相关;男性儿童中3种抗牛乳蛋白sIgG4水平在病例组与对照组的比值比均>20。结论与对照组相比,EoE患儿的抗牛乳蛋白sIgG4抗体的阳性率及滴度均增加。虽然尚不清楚这种抗体是否为EoE的致病因素,但sIgG4抗体的增加是引起EoE局部免疫应答的重要特征。

关键词：嗜酸性粒细胞性食管炎儿童 IgG4检测牛乳蛋白分子变应原

饮用水砷暴露与QT间期延长:砷对健康影响的纵向研究结果

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环境与职业医学 2013年第7期30卷 550-550页

作者： Yu Chen Fen Wu Faruque Parvez Alauddin Ahmed Mahbub Eunus Tyler R.McClintock Tazul Islam Patwary Tariqul Islam Anajan Kumar Ghosal Shahidul Islam Rabiul Hasan diane levy Golam Sarwar Vesna Slavkovich Alexander van Geen Joseph H.Graziano Habibul Ahsan

[背景]饮用水砷暴露与心脏疾病相关联,然而内在的机制尚不明确。[目的]评估饮用水砷暴露史与心率校正的QT(QTc)、PR和QRS间期延长之间的关联。[方法]从砷对健康影响的纵向研究中选择1715名基线入组的参加者进行研究。评估2005—2010年间... 详细信息

[背景]饮用水砷暴露与心脏疾病相关联,然而内在的机制尚不明确。[目的]评估饮用水砷暴露史与心率校正的QT(QTc)、PR和QRS间期延长之间的关联。[方法]从砷对健康影响的纵向研究中选择1715名基线入组的参加者进行研究。评估2005—2010年间,井水中的砷暴露和尿液样本与心电图(ECG)参数的关联,自基线开始平均跟踪了5.9年。[结果]QTc延长的定义为男性QTc≥450ms,女性≥460ms。井水中砷每增加1-SD(108.7μg/l),QTc延长的调整比值比(OR)为1.17(95%CI:1.01～1.35)。正相关仅限于女性,基线时井水和尿液中的砷每增加1-SD,女性调整后ORs分别比为1.24(95%CI:1.05～1.47)和1.24(95%CI:1.01～1.53);与之相比,男性调整后ORs分别为0.99(95%CI:0.73～1.33)和0.86(95%CI:0.49～1.51)。基线时井水砷或尿砷与女性或男性的PR或QRS间期延长之间无明显的关联。[结论]长期饮用水砷暴露(平均95μg/l,全距为0.1～790μg/l)与女性之后的QT间期延长相关联。在今后的纵向研究中,ECG的反复测量对于评估暴露变化的影响将具有重要意义。

关键词：砷孟加拉心血管疾病心电图心率校正的QT间期环境暴露

Immunity:研究开发出最优的HIV疫苗递送模式

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现代生物医学进展 2017年第27期17卷 I0003-I0003页

作者： Matthias Pauthner Colin Havenar-Daughton Devin Sok Joseph P. Nkolola Raiza Bastidas Archana V. Boopathy diane G. Carnathan Abishek Chandrashekar Kimberly M. Cirelli Christopher A. Cottrell Alexey M. Eroshkin Javier Guenaga Kirti Kaushik Daniel W. Kulp Jinyan liu laura E. McCoy Aaron l. Oom Gabriel Ozorowski Kai W. Post Shailendra K. Sharma Jon M. Steichen Steven W. de Taeye Talar Tokatlian Alba Torrents de la Peña Salvatore T. Butera Celia C. laBranche David C. Montefiori Guido Silvestri Ian A. Wilson Darrell J. Irvine Rogier W. Sanders William R. Schief Andrew B. Ward Richard T. Wyatt Dan H. Barouch Shane Crotty Dennis R. Burton University of California San Diego La Jolla CA 92093 USA

HIV已不再占据头条新闻。这主要是因为抗逆转录病毒药物的成功开发已将HIV病毒感染导致的艾滋病（AIDS）转化为一种慢性的可控制的疾病。然而。当前在全世界大约0．367亿名HIV感染者中，仅约一半的人能够获得控制这种病毒所需的药物。... 详细信息

HIV已不再占据头条新闻。这主要是因为抗逆转录病毒药物的成功开发已将HIV病毒感染导致的艾滋病（AIDS）转化为一种慢性的可控制的疾病。然而。当前在全世界大约0．367亿名HIV感染者中，仅约一半的人能够获得控制这种病毒所需的药物。与此同时。新的HIV感染率持续保持在非常高的水平，这突出强调了需要开发一种预防性疫苗。然而，几十年来。

关键词： HIV感染者研究开发疫苗递送病毒感染逆转录病毒艾滋病感染率