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Optimization of Technical Parameters for Detecting Mycobacteria in Hospital Wastewater in Tropical Urban Areas: The Case of the City of Abidjan (Côte d’Ivoire)

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Open Journal of Medical Microbiology 2023年第3期13卷 195-206页

作者： Cissé Souleymane Coulibaly-Kalpy Julien Vakou N’dri Sabine Assohoiun Egomli Stanislas Ouattara Mohamed Baguy Kouamé Kintossou Ambroise diané kouao maxime Ettien Money Marcelle Nguessan Felix Kouassi Dosso Mireille Department of the Biological Resource Center Institut Pasteur Abidjan C&#244te d’Ivoire Department of Environment and Health Institut Pasteur Abidjan C&#244 te d’Ivoire Laboratory of Microbial Genomics and Metagenomics Institut Pasteur Abidjan C&#244 te d’Ivoire Laboratory of Environmental Sciences and Technology Jean Lorougnon Guédé University Daloa C&#244te d’Ivoire Input Preparation Unit Institut Pasteur Abidjan C&#244te d’Ivoire Institut Pasteur Abidjan C&#244te d’Ivoire

The loads of organic matter, microorganisms, detergents and antibiotics in liquid hospital effluents make them complex environments, raising numerous health and ecological questions. Investigations of mycobacteria in water lack adequate techniques. This study is the first part of a pilot project aimed at developing an optimized protocol for the isolation of mycobacteria from hospital effluents, as a prelude to more in-depth investigation in this matrix. The aim was to compare the performance of two decontamination methods, three culture media and two incubation temperatures generally proposed in the literature, in order to identify the most effective methods in each case, as well as possible areas for improvement in the isolation of these germs from this environmental matrix. The results show that liquid hospital effluent can be decontaminated using both the NaOH method (4%;for 30 min.) and the CPC method (0.05%;for 30 min.), with the same mycobacteria recovery efficiency. Despite the low concentration, decontamination with CPC killed more mycobacteria and sufficiently eliminated contaminating germs. In contrast, decontamination with NaOH was less harmful to mycobacteria, but did not remove many contaminating germs. On the other hand, LJG medium performed better than LJGF medium and LJGP medium for the growth of mycobacteria in hospital waters. Finally, there was no difference in performance between the two incubation temperatures of 30℃ and 37℃. The results of this study show that further evaluation of existing protocols is required in order to optimize methods for the pre-treatment of hospital effluent for the isolation of mycobacteria.

关键词： Hospital Wastewater Mycobacterium Decontamination Löwenstein Jensen Medium Growth Temperature

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Capacity Development of National Reference Centers (NRC) to Face the Challenges of Emerging Infections in Côte d’Ivoire

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Open Journal of Internal Medicine 2023年第3期13卷 181-208页

作者： diané kouao maxime Adjogoua Edgard Valery Coulibaly Ngolo Antoine Marie-David Kadjo A. Hervé N’guessan Kouassi Raymond Sylla Yahaya Anne Blessa Jean Claude Beourou Sylvain Guessennd-Kouadio Aya Nathalie Dosso Mireille Tiembré Issiaka Pasteur Institute of Cô te d’Ivoire Abidjan C&ocircte d’Ivoire Biological Resource Center Abidjan C&ocircte d’Ivoire Epidemic Virus Department Abidjan C&ocircte d’Ivoire Molecular Biology Platform Abidjan C&ocircte d’Ivoire Mycobacterium Tuberculosis Unit Abidjan C&ocircte d’Ivoire Clinical Bacteriology Unit Abidjan C&ocircte d’Ivoire Department of Mycology Malariology Abidjan C&ocircte d’Ivoire Antibiotics Unit Abidjan C&ocircte d’Ivoire Department of Public Health UFR Medical Science Université Félix Houphou&eumlt-Boigny Abidjan C&ocircte d’Ivoire

Background: Some national and international strategies for the detection and prevention of emerging infectious diseases have been established across sectors. The capacity to carry out these tasks varies from country to country, and that remains largely undervalued. The Pasteur Institute of C?te d’Ivoire has created and implemented the capacity of national reference centers to fight against emerging and other infectious diseases. Objective: Show on the one hand the strategies used to develop the National Reference Centers and the IPCI and on the other hand the results obtained by performing these strategies. Method: Datas collection by documentary analysis (published scientific articles and grey literature) was done on Google Scholar, PUBMED and institutional reference documents. The documentary research was carried out to have a better understanding of strategies used to create and develop the NRCs in microbiology of communicable diseases. Results: Seven integrated strategies were launched: 1-training and workforce development;2-investigation of epidemics or public health events;3-strengthening laboratory epidemiological research;4-strengthening surveillance systems;5-improving communication with partners and stakeholders;6-building national and international collaborations;and 7-strengthening technical and technological platforms. In two decades, the number of researchers has risen from 10 in 2004 to ninety (90) in 2021, with 12 senior researchers and 32 junior researchers. A number of health service staff had attended a qualifying training course, 27 investigations into outbreaks and other public health events had been carried out, 18 short-term research projects had been launched, major surveillance programs and epidemiological research efforts on vector-borne, food-borne and nosocomial infections had begun, and several scientific manuscripts had been published or were edited in the writting press. Conclusion: The Ivorian experience shows that, with concerted eff

关键词： Evaluation Communicable Diseases National Reference Centerorlaboratorie Capacity Development Côte d’Ivoire

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Optimization of Technical Parameters for Detecting Mycobacteria in Hospital Wastewater in Tropical Urban Areas: The Case of the City of Abidjan (Côte d’Ivoire)

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Open Journal of Medical Microbiology（医学微生物学） 2023年第3期

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Capacity Development of National Reference Centers (NRC) to Face the Challenges of Emerging Infections in Côte d’Ivoire

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Open Journal of Internal Medicine（内科学期刊） 2023年第3期

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