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The response of structure and nitrogen removal function of the biofilm on submerged macrophytes to high ammonium in constructed wetlands

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Journal of Environmental Sciences 2024年第8期142卷 129-141页

作者： Xue Jiang Mengmeng Wang Shunqing Yang di He Fei fang Liuyan Yang State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210023China School of Resources and Environment Anqing Normal UniversityAnqing 246133China

The ammonium exceedance discharge from sewage treatment plants has a great risk to the stable operation of subsequent constructed wetlands(CWs).The effects of high ammonium shocks on submerged macrophytes and epiphytic biofilms on the leaves of submerged macrophytes in CWs were rarely mentioned in previous *** this paper,the 16S rRNA sequencing method was used to investigate the variation of the microbial communities in biofilms on the leaves of Vallisneria natans plants while the growth characteristics of *** plants were measured at different initial ammonium *** results demonstrated that the total chlorophyll and soluble sugar synthesis of *** plants decreased by 51.45%and 57.16%,respectively,and malondialdehyde content increased threefold after8 days if the initial NH_(4)^(+)-N concentration was more than 5 mg/*** density,bacterial quantity,dissolved oxygen,and pH increased with high ammonium *** average removal efficiencies of total nitrogen and NH_(4)^(+)-N reached 73.26%and 83.94%,*** heat map and relative abundance analysis represented that the relative abundances of phyla Proteobacteria,Cyanobacteria,and Bacteroidetes *** numbers of autotrophic nitrifiers and heterotrophic nitrification aerobic denitrification(HNAD)bacteria expanded in *** particular,HNAD bacteria of Flavobacterium,Hydrogenophaga,Acidovorax,Acinetobacter,Pseudomonas,Aeromonas,and Azospira had higher abundances than autotrophic nitrifiers because there were organic matters secreted from declining leaves of *** *** analysis of the nitrogen metabolic pathway showed aerobic denitrification was the main nitrogen removal ***,the nitrification and denitrification bacterial communities increased in epiphytic biofilms on submerged macrophytes in constructed wetlands while submerged macrophytes declined under ammonium shock loading.

关键词： Ammonium shock loading Biofilm Autotrophic nitrifiers Heterotrophic nitrification aerobic denitrification bacteria Submerged macrophyte

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Quantification of six bioactive compounds in Zhenqi Fuzheng preparation by high-performance liquid chromatography coupled with diode array detector and evaporative light scattering detector

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Journal of Pharmaceutical Analysis 2011年第1期1卷 20-25页

作者： Yi-Kai Shi fang Cui fang-di Hu Ying-Yan Bi Yu-Feng Ma Shi-Lan Feng School of Pharmacy Lanzhou University Lanzhou 730000 China Gansu Province Key Laboratory of Preclinical Study for New Drugs of Gansu Province Lanzhou 730000 China

A simple and accurate high-performance liquid chromatography（HPLC）coupled with diode array detector（DAD）and evaporative light scattering detector（ELSD）was established for the determination of six bioactive compounds in Zhenqi Fuzheng preparation（ZFP）.The monitoring wavelengths were 254,275 and 328 *** the optimum conditions,good separation was achieved,and the assay was fully validated in respect of precision,repeatability and *** proposed method was successfully applied to quantify the six ingredients in 31 batches of ZFP samples and evaluate the variation by hierarchical cluster analysis（HCA）,which demonstrated significant variations on the content of these compounds in the samples from different manufacturers with different preparation *** developed HPLC method can be used as a valid analytical method to evaluate the intrinsic quality of this preparation.

关键词： high-performance liquid chromatography(HPLC) diode array detector(DAD) evaporative light scattering detector(ELSD) Zhenqi Fuzheng preparation quantification hierarchical cluster analysis

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牛精液低温(0℃)保存的效果研究

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中国畜禽种业 2024年第3期20卷 11-16页

作者：史瑞莉何晓霞牛鹏龙瑞艳高庆华方翟塔里木大学动物科学与技术学院新疆阿拉尔843300 新疆鼎新种业科技有限公司新疆阿克苏842011 塔里木畜牧科技兵团重点实验室新疆阿拉尔843300

为研究一种Tris-卵黄稀释液0℃(冰水混合物中)保存牛精液的效果,该试验选用5头西门塔尔牛,将每头牛采集的精液等量分装,按1∶3的比例分别与试验组稀释液(Tris-卵黄专利稀释液)、对照组稀释液(基础稀释液)等温稀释处理,在0℃(冰水混合物... 详细信息

为研究一种Tris-卵黄稀释液0℃(冰水混合物中)保存牛精液的效果,该试验选用5头西门塔尔牛,将每头牛采集的精液等量分装,按1∶3的比例分别与试验组稀释液(Tris-卵黄专利稀释液)、对照组稀释液(基础稀释液)等温稀释处理,在0℃(冰水混合物中)状态下保存。检测保存0、1、2、4、6、8、10、12 d的精子活率、顶体完整率及质膜完整性,并用保存5 d的精液进行人工授精,统计受胎率。结果表明:保存4~10 d,A组稀释液保存的精子活率显著高于B组(P0.05)。A组稀释液保存牛精子的受胎率显著高于B组。可见,用Tris-卵黄稀释液0℃保存牛精液,保存效果较好,受胎率较高。

关键词：低温保存牛精液稀释液

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补肾化痰活血方联合针灸对多囊卵巢综合症患者促排卵及性激素水平的影响

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湖北中医药大学学报 2024年第2期26卷 30-33页

作者：邸志芳杨春香陈丽萍肖远德河北省康复医院河北秦皇岛066199 河北省衡水市第七人民医院河北衡水053800

目的观察补肾化痰活血方、针灸对多囊卵巢综合症(PCOS)患者促排卵及性激素水平的影响。方法将我院124例PCOS患者随机分为治疗组与对照组各62例。对照组口服克罗米芬治疗,治疗组在对照组的治疗基础上采用补肾化痰活血方联合针灸治疗。... 详细信息

目的观察补肾化痰活血方、针灸对多囊卵巢综合症(PCOS)患者促排卵及性激素水平的影响。方法将我院124例PCOS患者随机分为治疗组与对照组各62例。对照组口服克罗米芬治疗,治疗组在对照组的治疗基础上采用补肾化痰活血方联合针灸治疗。比较两组临床疗效,测定子宫内膜厚度、统计A型子宫内膜比例,测定性激素卵泡刺激素(FSH)、睾酮(T)、雌二醇(E2)、黄体生成素(LH)水平,比较优势卵泡数、成熟卵泡数、排卵率、妊娠率、多胎妊娠率,评估宫颈黏液Insler评分、统计Insler≥8分比例。结果治疗后,治疗组临床总有效率显著高于对照组(P0.05)。两组宫颈黏液Insler评分高于治疗前,治疗组宫颈黏液Insler评分高于对照组(P<0.05);治疗组Insler≥8分比例高于对照组(P<0.05)。结论补肾化痰活血方联合针灸治疗PCOS临床疗效显著,可促排卵,增加子宫内膜厚度,促妊娠,调节性激素水平,改善宫颈黏液性状。

关键词：多囊卵巢综合症排卵性激素水平补肾化痰活血方针灸

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Construction and expression of eukaryotic plasmids containing lamivudine-resistant or wild-type strains of Hepatitis B Virus genotype C

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World Journal of Gastroenterology 2008年第23期14卷 3733-3738页

作者： Wei-Zhen Xu Yong fang di Li Yan Wang Qing-Long Shang Gui-Qiu Li Xu Teng Hong-Xi Gu Department of Microbiology Harbin Medical University Harbin 150081 Heilongjiang Province China

AIM:To construct eukaryotic expression plasmids of full-length Hepatitis B Virus(HBV) genotype C genome,which contain lamivudine-resistant mutants(YIDD,YVDD) or wild-type strain(YMDD) ,and to observe the expression of HBV DNA and antigens [hepatitis B surface antigen(HBsAg) and hepatitis B e antigen(HBeAg) ] of the recombinant plasmids in HepG2 cells. METHODS:Three HBV full-length genomes were amplified from the plasmids pMD18T-HBV/YIDD,pMD18T-HBV/YVDD and pMD18T-HBV/YMDD,using PCR. Three recombinant plasmids were generated by inserting each of the PCR products into the eukaryotic expression vector pcDNA3.1(+) ,between the EcoRI and HindⅢ sites. After being characterized by restriction endonuclease digestion,and DNA sequence analysis,the recombinant plasmids were transfected into HepG2 cells. At 48 and 72 h post-transfection,the levels of intracellular viral DNA replication were detected by real-time PCR,and the expression of HBsAg and HBeAg in the cell culture supernatant was determined by ELISA. RESULTS:Restriction endonuclease digestion and DNA sequence analysis confirmed that the threerecombinant plasmids were correctly constructed. After transfecting the plasmids into HepG2 cells,high levels of intracellular viral DNA replication were observed,and HBsAg and HBeAg were secreted into the cell culture supernatant. CONCLUSION:Eukaryotic expression plasmids pcDNA3.1(+) -HBV/YIDD,pcDNA3.1(+) -HBV/YVDD or pcDNA3.1(+) -HBV/YMDD,which contained HBV genotype C full-length genome,were successfully constructed. After transfection into HepG2 cells,the recombinant plasmids efficiently expressed HBV DNA,HBsAg and HBeAg. Our results provide an experimental basis for the further study of HBV lamivudine-resistant mutants.

关键词： Hepatitis B virus Lamivudine-resistant mutant Wild-type strain

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Electrochemical study and application on rutin at chitosan/graphene films modified glassy carbon electrode

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Journal of Pharmaceutical Analysis 2013年第2期3卷 102-108页

作者： Jing An Ying-Yan Bi Chun-Xia Yang fang-di Hu Chun-Ming Wang School of Pharmacy Lanzhou University College of Chemistry and Chemical Engineering Lanzhou University

Graphene（G） was dispersed into 0.5% chitosan（Chit） solution,then the composite films were coated on glassy carbon electrode（GCE）,the electrochemical behavior of rutin on a Chit/G modified GCE was investigated and the electrochemical parameters of rutin were *** effectively accumulated on the Chit/G/GCE and caused a pair of redox peaks at around 408 mV and 482 mV（***） in 0.1M phosphate buffer solution（pH 4.0）.Under optimized conditions,the anodic peak current was linear to the rutin concentration in the range of 5×107-1.04×*** regression equation was：y 9.9219x-0.0025,r=*** proposed method was successfully used for the determination of rutin content in tablet samples with satisfactory results.

关键词： Electrochemical Chitosan/graphene films differential pulse voltammetry Rutin

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Network pharmacology unveils spleen-fortifying effect of Codonopsis Radix on different gastric diseases based on theory of “same treatment for different diseases” in traditional Chinese medicine

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Chinese Herbal Medicines 2021年第2期13卷 189-201页

作者： Ru-pu He Zheng Jin Ru-yun Ma fang-di Hu Jian-ye Dai School of Pharmacy Lanzhou UniversityLanzhou 730000China

Objective: "Same treatment for different diseases" is a unique treatment strategy under the guidance of traditional Chinese medicine(TCM) *** Radix(Codonopsis pilosula,Dangshen in Chinese)with spleen-fortifying effect was employed to understand the strategy of "Same treatment for different diseases",based on its common mechanism in the treatment of gastric diseases including gastric ulcer,gastritis and gastric cancer via network pharmacology ***:Network pharmacology research methods were used to analyze the interaction network and potential mechanisms of Dangshen in treating gastric ulcer,gastritis and gastric *** active components and their target proteins of Dangshen were integrated from TCMSP,BATMAN-TCM *** targets of gastric ulcer,gastritis and gastric cancer were collected through GeneCards,PubMed,TDD and disGeNET *** screening,the key components and the key targets of Dangshen in treating gastric ulcer,gastritis and gastric cancer were *** KEGG pathway analysis and GO analysis,the important pathways and biological processes were ***:Through data and literature mining,the common and specific pharmaceutical effects and mechanism of Dangshen were summarized in these three gastric *** was shown that Dangshen mainly acted on gastric ulcer,gastritis and gastric cancer through the overall regulation of the PI3K-AkT signaling *** the development of the disease,it will gradually increase the control of inflammation through TNF,NF-κB and other inflammation-related signaling pathways to reduce inflammatory *** tumorigenesis,it pays more attention to inhibiting the ErbB signaling pathways to reduce the proliferation and migration of tumor *** addition,Dangshen's regulation of HIF-1 signaling pathway may also be beneficial for the treatment of gastric ulcer,gastritis and gastric ***:Dangshen achieves spleen-fortifying effect on gastric diseases includi

关键词： Codonopsis Radix gastric cancer gastric ulcer gastritis network pharmacology same treatment for different diseases spleen-fortifying effect

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Helmholtz decomposition with a scalar Poisson equation in elastic anisotropic media

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Petroleum Science 2024年第3期21卷 1597-1610页

作者： Xin-Yu fang Gang Yao Qing-Qing Zheng Ping-Min Zhang di Wu Feng-Lin Niu State Key Laboratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing102249China Unconventional Petroleum Research Institute China University of Petroleum(Beijing)Beijing102249China Department of Mathematics College of ScienceChina University of Petroleum(Beijing)Beijing102249China College of Geophysics China University of Petroleum(Beijing)Beijing102249China Department of Earth Environmental and Planetary SciencesRice UniversityHoustonTX77005UA

P-and S-wave separation plays an important role in elastic reverse-time *** can reduce the artifacts caused by crosstalk between different modes and improve image *** addition,P-and Swave separation can also be used to better understand and distinguish wave types in complex *** present,the methods for separating wave modes in anisotropic media mainly include spatial nonstationary filtering,low-rank approximation,and vector Poisson *** of these methods require multiple Fourier transforms or the calculation of large matrices,which require high computational costs for problems with large *** this paper,an efficient method is proposed to separate the wave mode for anisotropic media by using a scalar anisotropic Poisson operator in the spatial *** 2D problems,the computational complexity required by this method is 1/2 of the methods based on solving a vector Poisson ***,compared with existing methods based on pseudoHelmholtz decomposition operators,this method can significantly reduce the computational *** examples also show that the P and S waves decomposed by this method not only have the correct amplitude and phase relative to the input wavefield but also can reduce the computational complexity significantly.

关键词： Anisotropic media Scalar anisotropic Poisson equation Improved elastic wavefield decomposition

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The pharmacokinetic study of rutin in rat plasma based on an electrochemically reduced graphene oxide modified sensor

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Journal of Pharmaceutical Analysis 2016年第2期6卷 80-86页

作者： PeiZhang Yu-Qiang Gou Xia Gao Rui-Bin Bai Wen-Xia Chen Bo-Lu Sun fang-di Hu Wang-Hong Zhao School of Pharmacy Lanzhou University Lanzhou 730000 China Lanzhou Military Command Center for disease Prevention and Control Lanzhou 730020 China Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou 510000 China

An electrochemical method based on a directly electrochemically reduced graphene oxide （ERGO） film coated on a glassy carbon electrode （GCE） was developed for the rapid and convenient determination of rutin in plasma. ERGO was modified on the surface of GCE by one-step electro-deposition method. Electrochemical behavior of rutin on ERGO/GCE indicated that rutin underwent a surface-controlled quasi-reversible process and the electrochemical parameters such as charge transfer coefficient （α）, electron transfer number （n） and electrode reaction standard rate constant （ks） were 0.53, 2 and 3.4 s -1, respectively. The electrochemical sensor for rutin in plasma provided a wide linear response range of 4.70 × 10 ^-7 1.25 × 10^-5 M with the detection limit （s/n=3） of 1.84 × 10^-8 M. The assay was success- fully used to the pharmacokinetic study of rutin. The pharmacokinetic parameters such as elimination rate half-life （t1/2）, area under curve （AUC）, and plasma clearance （CL） were calculated to be 3.345 ± 0.647 rain, 5750 ±656.0 μg min/mL, and 5.891± 0.458 mL/min/kg, respectively. The proposed method utilized a small sample volume of 10 μL and had no complicated sample pretreatment （without deproteinization）, which was simple, eco-friendly, and time- and cost-efficient for rutin pharmacokinetic studies.

关键词： Electrochemically reduced graphene oxide Pharmacokinetics Rat plasma Rutin

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FMP程序系统的微机化改造和验证计算

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原子能科学技术 2013年第B6期47卷 164-167页

作者：荣春方张棣芳中国原子能科学研究院反应堆工程研究设计所北京102413

为满足某研究项目的需要,对我国原有在CHALLENGE工作站上运行的燃料管理程序系统FMP进行微机化改造,大幅提高了该程序系统的计算速度和程序可移植性。该程序系统中两个主要程序TPFAP-E和CFMP的计算结果与原程序计算结果的比较表明,程序... 详细信息

为满足某研究项目的需要,对我国原有在CHALLENGE工作站上运行的燃料管理程序系统FMP进行微机化改造,大幅提高了该程序系统的计算速度和程序可移植性。该程序系统中两个主要程序TPFAP-E和CFMP的计算结果与原程序计算结果的比较表明,程序系统的微机化改造非常成功。在此基础上,针对堆芯设计计算报告《秦山300MWe核电厂堆芯第十次换料后续循环换料方案设计》的内容进行4个循环的燃料管理验证计算,主要参数计算结果与原报告的计算结果均吻合得较好,为利用该程序系统开展深入的燃料管理研究奠定了基础。

关键词：程序系统FMP 微机化改造燃料管理验证

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