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Screening, structural characterization and anti-adipogenesis effect of a water-soluble polysaccharide from Lycium barbarum L. by an activity-oriented approach

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FOOD BIOSCIENCE 2023年第3期53卷 409-420页

作者： Wang, Han Li, Yunchun Dai, Yunyun Ma, Ling di, duolong Liu, Jianfei Chinese Acad Sci Lanzhou Inst Chem Phys CAS Key Lab Chem Northwestern Plant Resources Key Lab Nat Med Gansu Prov Lanzhou 730000 Peoples R China Ningxia Inst Drug Control Intersect Ningan Santa Yinchuan 750001 Peoples R China Chinese Acad Sci Lanzhou Inst Chem Phys Key Lab Chem Northwestern Plant Resources 18 Tianshui Middle Rd Lanzhou 730000 Peoples R China Chinese Acad Sci Lanzhou Inst Chem Phys Key Lab Nat Med Gansu Prov 18 Tianshui Middle Rd Lanzhou 730000 Peoples R China

This study aimed to describe the structural features and anti-adipogenesis effect of polysaccharides obtained from Lycium barbarum L. by a bioactivity-oriented purification method. First, crude polysaccharide LICP009 was identified as the main bioactive compound with potential inhibition of intracellular lipid accumulation. Bioac-tivity guided fractionation was further performed using ion-exchange chromatography and Sephadex G-50 to obtain the purified component LICP009-2-1. This component was mainly composed of arabinose (Ara), galactose (Gal), glucose (Glc), galacturonic acid (GalA), and glucuronic acid (GlcA) in a molar ratio of 40.2:31.5:4.2:5.8:5.7 with a molecular weight of 1.35 x 104 g/mol. Linkage and NMR data indicated that the backbone of LICP009-2-1 was consisted of a repeated unit of -> 3,6)-beta-D-Galp-(1 -> residues, with three branches composed of alpha-L-Araf-(1 ->, alpha-L-Araf-(1 -> 5)-alpha-L-Araf-(1 -> 5)-alpha-L-Araf-(1 -> 3)-beta-D-Galp-(1 ->, and alpha-L-Araf-(1 -> 2)-alpha-L-Araf-(1 ->[4)-beta-D-Galp-(1]3 -> . In differentiated 3T3-L1 adipocyte model, LICP009-2-1 exhibited an antiadipogenic effect by decreasing lipid accumulation, as well as downregulating the expression of key tran-scription factors and proteins in the adipogenesis pathway in a dose-dependent manner. In high-fat-diet-induced obese mice, oral administration of LICP009-2-1 counteracted the progression of obesity and hyperlipidemia. These results indicated that LICP009-2-1 plays anti-adipogenic and anti-obesity roles both in vitro and in vivo, and could be an anti-obesity agent utilized as medicinal, pharmacological and functional food ingredient.

关键词： Lycium barbarum L Heteropolysaccharide Anti-adipogenesis Structural characterization

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Study on pharmacokinetics and tissue distribution of the isocorydine derivative (AICD) in rats by HPLC-DAD method

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Acta Pharmaceutica Sinica B 2015年第3期5卷 238-245页

作者： Yali Chen Qian Yan Mei Zhong Quanyi Zhao Junxi Liu duolong di Jinxia Liu Institute of Medicinal Chemistry School of PharmacyLanzhou University Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of Sciences Institute of Biology Gansu Academy of Sciences

A simple and effective high-performance liquid chromatography with diode-array detection method coupled with a liquid liquid extraction pretreatment has been developed for determining the pharmacokinetics and tissue distribution of a novel structurally modified derivative (8-acetamino-isocorydine) of isocorydine. According to the in vivo experiments data calculations by DAS 2.0 software, a two compartment metabolic model was suitable for describing the pharmacokinetic of 8-acetaminoiso-corydine in rats. 8-Acetamino-isocorydine was absorbed well after oral administration, and the absolute bioavailability was 76.5%. The half-life of 8-acetamino-isocorydine after intravenous and oral administration was 2.2 h and 2.0 h, respectively. In Oro, 8-acetamino-isocorydine was highly distributed in the lungs, kidney and liver; however, relatively little entered the brain, suggesting that 8-acetaminoisocorydine could not easily pass through the blood brain bather. Our work describes the first characterization of the pharmacokinetic parameters and tissue distribution of 8-acetamino-isocorydine. The acquired data will provide useful infonnation for the in vivo pharmacology of 8-acetaminoisocorydine, and can he applied to new drug research. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia IMedica, Chinese Academy of 'Medical Sciences. Production and hosting by Elsevier B.V.

关键词： Alkaloids Pharmacokinetics Tissue distribution High-performance liquid chromatography with didode-array detection 8-Acetaminao-isocorydine

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Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum

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Food Bioscience 2022年第3期47卷 679-687页

作者： Wenjin Ma Yanbing Zhou Wenjin Lou Bo Wang Bing Li Xiaofen Liu Jiajun Yang Bo Yang Jianfei Liu duolong di CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou730000China The Institute of Gansu Province Light Industrial Scientific Research Lanzhou730000China Gansu Provincial Academic Industrial for Medical Research Lanzhou730000China School of Life Science and Engineering Lanzhou University of TechnologyGansu ProvinceLanzhou730050China

The water-soluble polysaccharide LBP-1 was isolated and characterized from Lycium barbarum ***-1 was mainly composed of arabinose,galactose,glucose,xylose,mannose at a molar ratio of 37.53:28.08:14.72:7.83:4.50,*** on nuclear magnetic resonance(NMR)and methylation analysis,the backbone of LBP-1 was speculated in theα-L-Ara(1→[5-α-L-Ara(1→3)-β-D-Galp-(1→]n→4)-α-D-Galp-(1[→5-α-L-Ara(1]n[→6)-β-D-Galp-(1→4)-β-D-Galp-(1→]n,and the side chains of LBP-1 were in theα-L-Ara(1→3)-β-D-Galp-(1→6 *** results showed that LBP-1 inhibited the growth of cancer A549 cells through cell cycle arrest and apoptosis,with an IC50 value of 42.5μg/*** addition,LBP-1 altered the expression of Cyclin D1,Cyclin D3,and CDK 2,thus blocking the cell cycle in G0/G1 phase,reducing cell migration,and regulating the PI3K/Akt/mTOR signaling pathway to induce apoptosis.

关键词： Lycium barbarum polysaccharide Lung cancer cell Apoptosis Cell cycle Structural analysis

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Effect of Occupational Manganese Exposure on Uric Acid Levels in Human Urine

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Biomedical and Environmental Sciences 2011年第3期24卷 222-227页

作者： SUN XiaoMing LIU YeWei WANG ZhenQuan LI HongBing LIU Yun di duolong Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 Gansu China Graduate University of the Chinese Academy of Sciences Beijing 100049 China Institute of Nutrition and Food Hygiene School of Public Health Lanzhou University Lanzhou 730000 Gansu China

Objective To investigate the effect of long-term and low-level occupational Mn exposure on the level of uric acid （UA） in human urine. Methods In this study, 65 volunteers were recruited, who were working on welding and foundry work in an plant in Gansu province, China. Additionally, 29 control samples were collected from individuals who did not have any history of excessive Mn exposure. An improved high performance liquid chromatography system equipped with a diode-array detector （HPLC-DAD） method was developed to determine the UA level in human urine. A Spectra AA 220 Atomic Absorption Spectrophotometer （AAS） was used to measure the Mn level in the urine. Results The analytical method was validated acceptable accuracy, precision, and recovery significantly lower than that of control samples for concentrations ranging from 3.82-45.84 pg/mL with Overall, the UA levels of Mn exposure samples were （P〈O.O5）. Conclusion The practical method developed here is suitable for both routine monitoring of UA level in human urine and metabolism research. Long-term and low-level occupational Mn exposure may lead to a lower UA level in urine, and UA might be an indicator of the early stage of manganism.

关键词： High-performance liquid chromatography Uric acid Occupational Mn exposure

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Effects of different Wall-breaking Methods on Extraction of Solanesol from Stems and Leaves of Potato

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Agricultural Biotechnology 2017年第2期6卷 11-14页

作者： Bingpeng WANG Jing MENG Mei GUO Xinyi HUANG Fangyuan DONG duolong di Department of Pharmacy Gansu University of Medicine/Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu ProvinceLanzhou 730000China Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Comprehensive Development Office of Agriculture in Ningxia Au-tonomous Region Yinchuan 750011China

In order to extract solanesol from potato stems and leaves more effectively and improve the extraction rate of solanesol, the same batch of potato stems and leaves harvested from Guyuan, Ningxia was selected as a research object, cell wall of potato stems and leaves was broken by enzymolysis with cellulase and high- speed shearing, and then reflux-extracted with 95% ethanol. Solanesol content was determined by HPLC, and extract yield was calculated. Extract yield and solanesol extraction rate was used as an index for comparison of difference between the 2 wall-breaking methods, so as to select the optimal wall-breaking method. The results showed that enzymolysis with cellulase exhibited extraction rate and extract yield of solanesol of 91.38% and 8.02%, respectively, which were better than those under high-speed shear emulsification technique. The enzymolysis wall-breaking method has the advantages of simple operation and strong feasibility.

关键词： Enzymolysis High-speed shearing Potato stems and leaves Solanesol

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枸杞属植物中生物碱类成分研究进展

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科学通报 2022年第4期67卷 332-350页

作者：刘建飞巩媛杨军丽邸多隆中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室兰州730000 中国科学院大学北京100049

枸杞属(Lycium L.)属于茄科(Solanaceae)茄族(Solaneae)枸杞亚族(Lyciinae),在我国有7个种和3个变种.其中宁夏枸杞(*** L.)的果实“枸杞子”是我国名贵中药材,具有滋补肝肾、益精明目的传统功效,也是享誉古今中外的滋补佳品.枸杞属植物... 详细信息

枸杞属(Lycium L.)属于茄科(Solanaceae)茄族(Solaneae)枸杞亚族(Lyciinae),在我国有7个种和3个变种.其中宁夏枸杞(*** L.)的果实“枸杞子”是我国名贵中药材,具有滋补肝肾、益精明目的传统功效,也是享誉古今中外的滋补佳品.枸杞属植物具有调节免疫力、抗氧化、缓解体力疲劳、抗阿尔茨海默病、抗败血症、保护生殖系统、保护神经系统、防治眼部疾病、增强造血功能、提高性能力等功效.主要化学成分包括多糖、类胡萝卜素、黄酮、生物碱、苯丙素以及其他小分子和微量元素,其中生物碱被认为是主要活性成分之一.本文主要针对枸杞属植物中已报道的124个生物碱类化学成分及其生物活性进行总结分析和展望,为枸杞传统药用功效的现代药物研究和临床应用,以及枸杞属植物资源的高值化可持续利用提供科学参考.

关键词：枸杞属生物碱分子结构生物活性

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高速逆流色谱分离纯化枸杞多糖在神经炎症中的作用

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食品工业科技 2024年第20期45卷 68-77页

作者：郝高峰曹妤赵宁刘建飞邸多隆甘肃中医药大学药学院甘肃兰州730000 中国科学院兰州化学物理研究所甘肃兰州730000

目的:构建高速逆流色谱技术分离纯化枸杞多糖的新方法,并对分离得到的枸杞多糖进行结构表征,探究其对神经炎症的影响。方法:通过分配系数K值和固定相保留率Sf,筛选并优化双水相体系偶联高速逆流色谱分离纯化枸杞多糖的条件,利用高效分... 详细信息

目的:构建高速逆流色谱技术分离纯化枸杞多糖的新方法,并对分离得到的枸杞多糖进行结构表征,探究其对神经炎症的影响。方法:通过分配系数K值和固定相保留率Sf,筛选并优化双水相体系偶联高速逆流色谱分离纯化枸杞多糖的条件,利用高效分子排阻色谱联用激光光散射仪和示差检测器串联检测法、1-苯基-3-甲基-5-吡唑啉酮柱前衍生结合高效液相色谱法、傅立叶变换红外光谱仪、热重分析仪、扫描电子显微镜对枸杞多糖进行初步结构表征,利用脂多糖诱导的小胶质细胞炎症模型研究枸杞多糖对神经炎症的作用。结果:确定最佳双水相体系为乙醇、饱和硫酸铵、水、异丙醇的质量比为1.4:1.0:2.58:0.3,高速逆流色谱最佳分离纯化条件为转速900 r/min,流动相流速0.8 mL/min。在上述条件下,分离得到3个枸杞多糖组分LBPs-1、LBPs-2和LBPs-3,多糖含量分别为78.35%±1.52%,69.03%±1.71%和62.11%±2.31%,分子量分别为7.40×10^(4) Da,2.73×10^(4) Da和1.47×10^(4) Da,单糖组成分别为甘露糖:鼠李糖:半乳糖醛酸:葡萄糖醛酸:葡萄糖:半乳糖:阿拉伯糖,其中摩尔比分别为1.9:4.9:1.3:8.1:7.6:32.6:41.8、2.1:4.3:1.5:8.3:5.1:28.6:37.9和5.9:4.6:1.1:0.7:13.8:66.8:5.3,同时热稳定性和微观形貌也有明显差异。生物活性研究结果表明,不同分子量的枸杞多糖可通过抑制炎症因子分泌,缓解脂多糖诱导的小胶质细胞炎症。结论:本研究构建了双水相体系偶联高速逆流色谱分离纯化枸杞多糖的新方法,为植物多糖高效分离纯化提供了技术支撑,为枸杞多糖在抑制神经炎症相关疾病中的应用提供了科学依据。

关键词：高速逆流色谱双水相体系枸杞多糖分离纯化结构解析神经炎症

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富含羟基酪醇橄榄油对高血脂小鼠的影响

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食品科学 2023年第21期44卷 144-150页

作者：叶文斌陈文东王昱张凯黄新异邸多隆陇南师范高等专科学校农林技术学院甘肃成县742500 中国科学院兰州化学物理研究所甘肃兰州730000

研究富含羟基酪醇橄榄油(olive oil rich in hydroxyl tyrosol,OHT)对高血脂小鼠血糖和血脂的影响。以脂肪乳剂和75%(体积分数)的蛋黄乳灌胃构建小鼠高血糖高血脂模型,将建模成功的小鼠随机分为高血脂模型组,OHT高、中、低剂量(200、100... 详细信息

研究富含羟基酪醇橄榄油(olive oil rich in hydroxyl tyrosol,OHT)对高血脂小鼠血糖和血脂的影响。以脂肪乳剂和75%(体积分数)的蛋黄乳灌胃构建小鼠高血糖高血脂模型,将建模成功的小鼠随机分为高血脂模型组,OHT高、中、低剂量(200、100、50 mg/kg m_(b))组,普通橄榄油(olive oil,OL)组,菲勒贝特阳性对照(philbert,PL)组,以生理盐水为CK(0)组。连续灌胃给药28 d,分析OHT对高血脂小鼠空腹血糖(fasting blood glucose,FBG)、糖化血红蛋白(glycated hemoglobin,HbA1c)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白(high-density lipoprotein,HDL)、低密度脂蛋白(low-density lipoprotein,LDL)、总胆红素(total bilirubin,TBIL)、总胆汁酸(total bile acid,TBA)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬酸氨基转移酶(aspartate aminotransferase,AST)、γ-氨酰基转移酶(gammaammonia acyltransferase,GGT)、碱性磷酸酶(alkaline phosphatase,ALP)水平的影响,并对OHT开展急慢性毒理研究。结果显示,与模型组相比,OHT能有效降低高血脂小鼠的FBG、TC、TG、LDL、TBIL、TBA浓度及HbAlc相对含量,提高HDL浓度,对ALT、AST、GGT和ALP活性具有很好的抑制作用,能够改善血糖血脂代谢紊乱,还具有降低体质量的效果,且呈剂量效应关系。OHT对正常小鼠无急慢性毒理作用,能提高小鼠血清超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,降低丙二醛(malondialdehyde,MDA)浓度,提高小鼠的抗氧化能力,控制小鼠体质量。综上,OHT对高血脂小鼠具有降血糖、血脂作用,且无毒。本研究结果可为利用橄榄油开发降血脂产品提供参考。

关键词：羟基酪醇橄榄油高血脂小鼠降血糖降血脂急性和慢性毒理

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烟叶中茄尼醇的超声提取及纯化工艺研究

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中国药房 2016年第7期27卷 932-935页

作者：张艺王刚陈振斌邸多隆兰州理工大学材料科学与工程学院兰州730050 甘肃省有色金属新材料省部共建国家重点实验室兰州730050 中国科学院兰州化学物理研究所兰州730000

目的：优化烟叶中茄尼醇的超声提取及纯化工艺。方法：分别以茄尼醇提取率和转移率为指标,采用单因素试验对提取工艺中的料液比、超声提取温度、超声提取时间、超声功率、提取次数,以及纯化工艺中的皂化液用量（皂化液与提取液体积比）... 详细信息

目的：优化烟叶中茄尼醇的超声提取及纯化工艺。方法：分别以茄尼醇提取率和转移率为指标,采用单因素试验对提取工艺中的料液比、超声提取温度、超声提取时间、超声功率、提取次数,以及纯化工艺中的皂化液用量（皂化液与提取液体积比）、酸化液用量（酸化液与皂化后提取液体积比）、萃取次数等因素水平进行考察,对优化后工艺进行验证,计算茄尼醇纯度;另将本法与传统浸提法（耗时30 h）和溶剂连续循环浸提法（耗时5~6 h）比较茄尼醇的提取量。结果：优化的提取工艺为料液比1∶14、超声提取温度70℃、超声提取时间60 min、超声功率120 W、提取3次,纯化工艺为皂化液与提取液体积比2∶35、酸化液与皂化后提取液体积比2∶14、萃取4次;验证试验中提取率、转移率和纯度均值分别为92.45%、79.88%、55.86%（RSD分别为0.46%、0.30%、0.40%,n=3）;3种提取方法茄尼醇的提取量分别为52.22、45.22、26.10 mg/g。结论：优化工艺操作简单、稳定,提取耗时更短,更节约能源,且提取量和纯度均较高,适合用于烟叶中茄尼醇的生产提取及纯化。

关键词：烟叶茄尼醇单因素试验超声提取纯化工艺优化提取率转移率纯度

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鱼皮低聚肽在大孔吸附树脂上的吸附性能研究

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食品工业 2018年第3期39卷 34-39页

作者：刘永峰刘毅裴栋魏鉴腾邸多隆中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室兰州730000 青岛市资源化学与新材料研究中心青岛266100

为研究鱼皮低聚肽在大孔吸附树脂上的吸附性能,以鱼皮低聚肽为研究对象,以吸附容量和解吸附容量为评价指标,筛选出ANKX-2为最佳大孔吸附树脂,并利用等温线模型和动力学模型,系统研究了鱼皮低聚肽在ANKX-2上的吸附性能。吸附热力学结果表... 详细信息

为研究鱼皮低聚肽在大孔吸附树脂上的吸附性能,以鱼皮低聚肽为研究对象,以吸附容量和解吸附容量为评价指标,筛选出ANKX-2为最佳大孔吸附树脂,并利用等温线模型和动力学模型,系统研究了鱼皮低聚肽在ANKX-2上的吸附性能。吸附热力学结果表明,鱼皮低聚肽在ANKX-2上的吸附等温线属于IUPAC的6类等温线中的类型Ⅱ;该吸附过程是可行的、有利的,是多层物理吸附过程,是自发、放热和熵减小的过程。吸附动力学结果表明,鱼皮低聚肽在ANKX-2的吸附过程属于快速吸附类型,准二级动力学模型可较好地拟合试验数据。研究结果可为利用大孔吸附树脂富集鱼皮低聚肽的工艺研究提供技术支持,也为利用大孔吸附树脂制备鱼皮低聚肽的工业化生产提供科学依据。

关键词：大孔吸附树脂鱼皮低聚肽吸附性能吸附热力学吸附动力学

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