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Phyton-International Journal of Experimental Botany 2022年第4期91卷 761-772页

作者： Yizhao Huang Nannan Li deya wang Jian Du Weitang Liu Jinxin wang Wei Li College of Food Science and Pharmaceutical Engineering Zaozhuang UniversityZaozhuang277160China Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing100093China Key Laboratory of Pesticide Toxicology and Application Technique College of Plant ProtectionShandong Agricultural UniversityTai’an271018China College of Life Sciences Zaozhuang UniversityZaozhuang277160China

Beckmannia syzigachne is a noxious weed for rice-wheat rotations in *** ***(AH-02)population evolved metabolic resistance to fenoxaprop-P-ethyl and *** investigate the function of GT73C1 in this population,the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction,and the sequence was 100%consistent with the transcriptome *** phylogenetic tree was displayed and annotated using FigTree *** plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon ***,the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl,which was consistent with the findings from the whole plant *** results indicate that GT73C1 is closely related to the metabolic resistance of ***.

关键词： Beckmannia syzigachne GT73C1 gene phylogenetic analyses Brachypodium distachyon genetic transformation

Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194

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Phyton-International Journal of Experimental Botany 2019年第2期88卷 109-117页

作者： Qunqun Hao Bo Lyu Yuhan Tang deya wang Yuanyuan Li Qingliang Li Yuhai wang Wenqiang wang College of Life Sciences Zaozhuang UniversityZaozhuang277000China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an271018China.

A barley mutant,194,was observed to exhibit a leaf spot phenotype over the whole course of its growing *** this study,the phenotype and antioxidant competence were studied in the lesion mimic mutant *** height was slightly higher in mutant 194 than in the wild type(WT).In addition,leaf spot per plant in mutant 194 was significantly higher than in *** competence,as indicated by reactive oxygen species(ROS)accumulation,antioxidant enzyme activity,and the expression of antioxidant enzyme-encoding genes was also assessed in mutant *** to the WT,mutant 194 displayed a relatively higher accumulation of ROS,accompanied by lower activities of some antioxidant enzymes and downregulation of antioxidant enzyme-encoding *** demonstrated reduced antioxidant competence in mutant *** results suggested that this lower antioxidant competence of mutant 194 could lead to the accumulation of excessive *** excess of ROS could induce programmed cell death and has the potential to promote disease resistance in mutant 194.

关键词： Lesion mimic mutant ROS accumulation antioxidant enzymes barley

Analysis of Subcellular Localization and Pathogenicity of Plum Bark Necrosis Stem-Pitting Associated Virus Protein P6

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2079-2085页

作者： Yuanyuan Li Jinze Mu Qingliang Li Huabing Liu Xuefeng Yuan deya wang College of Life Sciences Zaozhuang UniversityZaozhuang Cherry Virus Disease Rapid Diagnosis and Green Control Technology Innovation CenterZaozhuang277160China College of Plant Protection Shandong Agricultural UniversityShandong Agricultural Microbiology Key LaboratoryTai’an271018China Yicheng Agricultural and Rural Comprehensive Service Center Zaozhuang277300China

Infection of plum bark necrosis stem pitting associated virus(PBNSPaV)has been reported in many Prunus species in several countries,causing significant economic *** very small proteins encoded by plant viruses are often overlooked due to their short sequences and uncertain ***,numerous studies have indicated that they might play important roles in the pathogenesis of virus *** role of small hydrophobic protein P6,encoded by the open reading frame 2 of PBNSPaV,has not been well *** this study,we amplified the P6 fragment from a PBNSPaV isolate by RT-PCR using specific primers and found that it is 174 bp long and encodes a protein of approximately 6.3 kD with a transmembrane *** localization analysis of P6 proteins in tobacco leaves showed that P6 localizes to the cytomembrane and nuclear *** further clarify the pathogenicity of P6 proteins,we constructed a PVX-P6 expression vector by inserting the p6 fragment into a potato virus X(PVX)-based vector and transformed it into Agrobacterium tumefaciens *** of Nicotiana benthamiana(***)with the PVX vector-transformed *** led to slight mosaic symptoms at 14 days of ***,infiltration with the PVX-P6 vector-transformed *** resulted in no significant *** results demonstrated that heterologous expression of P6 in *** could not enhance the pathogenicity of *** study indicates that P6 may not be a potential pathogenic factor associate with the causing of symptoms,and the mode of action of PBNSPaV-P6 protein remains to be further studied.

关键词： Plum bark necrosis stem-pitting associated virus P6 protein subcellular localization pathogenicity

Cis-elements of-1 programmed ribosome frameshift responsible for the expression of RdRp in Tobacco bushy top virus

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Cis-elements of-1 programmed ribosome frameshift responsible...

Cap-independent translation of ORF1（p35）encoded by Tobacco bushy top virus

中国植物病理学会第十二届青年学术研讨会

作者： Chengming Yu deya wang Guolu wang Xuefeng Yuan College of Plant Protection Shandong Agricultural University

Tobacco bushy top virus（TBTV） has a（+） ssRNA genome with 4 152 *** viral genome without 5-cap and 3-poly（A） encoded four putative open reading frames（ORFs）,in which ORF1（p35） and ORF2（p63） are components of replicase based on common charactericstic of *** in vitro translation of TBTV in wheat germ extract（WGE）,it showed that ORF2（p63） was expressed through-1 type frameshift mechanism and existed as ORFl-fused pattern resulting in the production of p98,which may function as RNA-dependent RNA polymerase（RdRp）.Through comparation of sequences,mutagenesis and in vitro translation,we identified the essential heptanucleotide slippery sequences at946 GGATTTT,whose site-mutations decreased the frameshift ratio to about 30%of wild *** also tested the optimal distance between heptanucleotide slippery sequences and potential RNA structure located 6 nt downstream,showing that 9 nt is the upper ***,long distance RNA-RNA interaction was found though electrophoretic mobility shift assay（EMSA）.The 3’ terminal 200 nt could form RNA-RNA interaction with potential RNA structure located downstream of heptanucleotide slippery ***-line probing showed that one of the RNA-RNA interaction sites is located at the 3’ proximal hairpin,which is also responsible for replication in similar *** local RNA structure and detailed long distance RNA-RNA interaction,which is essential for-1type frameshift,will be further identified.

关键词： Tobacco bushy top virus ribosomal frameshift RNA-RNA interaction in-line probing

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Cap-independent translation of ORF1（p35）encoded by Tobacco...

植物RNA病毒不依赖帽子翻译机制的研究进展

中国植物病理学会第十二届青年学术研讨会

作者： deya wang Chengming Yu Guolu wang Xuefeng Yuan College of Plant Protection Shandong Agricultural University

Tobacco bushy top virus（TBTV）,a member of Umbravirus,contains a positive single genomic RNA with 4 152 *** genome of TBTV lacked 5’ cap and3 poly（A） tail and encoded 4 proteins including components of replicase（p35 and p63）,and movement proteins（p26 and p27）.For the cap-independent translation of p35,previous report showed the existence of a BTE-like element in TBTV by using firefly luciferase（Fluc） reporter *** confirmed the BTE-like element in fulllength TBTV through in vitro translation in wheat germ extract（WGE）.Furthermore) we used forward and reverse genetics methods to identify new elements regulating the expression of *** expression of p35 among different TBTV isolates showing severe or mild pathogenicity had 3 fold ***,the regions altering the expression of p35 were mapped within 5’ terminal 500 nt and internal600 nt regions surrounding BTE-like elements by constructing chimeric *** 3proximal 120 nt regions located downstream of BTE-like element were also played role in the translation of p35 by deletion *** 3 terminal 120 nt regions contained 5 hairpins and three RNA pseudoknots through in-line probing and their roles on the translation of p35 will be further *** a word,it is suggested that at least three cis-elements including BTE-like element were associated with the cap-independent translation of p35.

关键词： Tobacco bushy top virus cap-independent translation BTE-like element in-line probing

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病毒学报 2017年第4期33卷 652-660页

作者：王德亚原雪峰山东农业大学植物保护学院植物病理系山东省农业微生物重点实验室泰安271018

真核生物系统中,mRNA 5′末端帽子结构通过结合翻译起始因子eIF4E进而招募40S核糖体亚基,对蛋白翻译的起始调控至关重要,而3′末端poly(A)尾则通过介导mRNA环化提高翻译效率。然而,许多植物RNA病毒的基因组缺乏5′帽子结构,甚至3′末端... 详细信息

真核生物系统中,mRNA 5′末端帽子结构通过结合翻译起始因子eIF4E进而招募40S核糖体亚基,对蛋白翻译的起始调控至关重要,而3′末端poly(A)尾则通过介导mRNA环化提高翻译效率。然而,许多植物RNA病毒的基因组缺乏5′帽子结构,甚至3′末端也不带poly(A)尾,这些病毒进化产生了位于基因组RNA5′末端或3′末端的不依赖帽子翻译元件。本文将重点讨论位于植物病毒基因组RNA 3′末端的不依赖帽子翻译元件(Cap-independent Translation Element,3′CITE),就其结构特征、调控翻译起始和RNA环化等方面的研究进展进行了综述。

关键词：植物RNA病毒帽子不依赖帽子翻译元件 RNA环化

基于卷积神经网络的两相复合结构动态力学性能研究

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高压物理学报 2023年第3期37卷 95-107页

作者：卜乐虎王鹏飞武扬帆王德雅徐松林中国科学技术大学中国科学院材料力学行为和设计重点实验室安徽合肥230027

增材制造技术促进了复合材料的发展,也拓宽了复合结构的设计空间,然而基于增材制造的复合材料动态力学性能研究仍然面临研究方法欠缺、设计过程复杂等问题。利用分离式霍普金森压杆实验技术和ABAQUS有限元模拟,研究光固化3D打印两相复... 详细信息

增材制造技术促进了复合材料的发展,也拓宽了复合结构的设计空间,然而基于增材制造的复合材料动态力学性能研究仍然面临研究方法欠缺、设计过程复杂等问题。利用分离式霍普金森压杆实验技术和ABAQUS有限元模拟,研究光固化3D打印两相复合材料的动态力学行为,结合主成分分析法建立复合结构的数据集,通过高性能的卷积神经网络学习复合材料结构与应力-应变曲线的关系。结果表明,含有界面单元的有限元模型更适用于模拟复合材料的动态力学响应,通过超参数的设置可以提高卷积神经网络的预测性能,训练完成的卷积神经网络能够根据结构快速预测复合材料的动态应力-应变曲线。此研究对机器学习在复合材料动态力学性能设计与应用具有一定的借鉴意义。

关键词：复合材料 3D打印主成分分析卷积神经网络动态应力-应变曲线

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单丝碳纳米管纤维的横向冲击性能研究

实验力学 2021年第4期36卷 507-515页

作者：常晋源王德雅张磊王鹏飞徐松林中国科学技术大学中国科学院材料力学行为和设计重点实验室安徽合肥230027

由于碳纳米管(CNT)纤维具有优异的力学和电学性能,使其成为人工肌肉、超级电容器和耐撞结构的理想材料。但由于纤维单丝的力学信号较弱,对其横向冲击失效行为的研究尚欠缺。本文基于高精度力传感器发展了纤维单丝在横向冲击过程中的信... 详细信息

由于碳纳米管(CNT)纤维具有优异的力学和电学性能,使其成为人工肌肉、超级电容器和耐撞结构的理想材料。但由于纤维单丝的力学信号较弱,对其横向冲击失效行为的研究尚欠缺。本文基于高精度力传感器发展了纤维单丝在横向冲击过程中的信号测量装置,利用该装置结合扫描电镜对比研究了尼龙纤维和碳纳米管纤维的横向冲击特性。研究结果表明:碳纳米管纤维在受到横向冲击时,将由黏弹性拉伸阶段发展到强化拉伸阶段,之后达到断裂极限而失效;碳纳米管纤维的冲击失效对应了更快的载荷增长速度和更短的加载时长。由扫描电镜可以观察到,在较高的冲击速度下纤维截面断口更整齐。

关键词：冲击动力学横向冲击碳纳米管(CNT) 单丝纤维脆性断裂

Swarm intention identification via dynamic distribution probability image

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Chinese Journal of Aeronautics 2024年第10期37卷 380-392页

作者： Yinhan wang Jiang wang Shaoming HE Fei wang Qi wang School of Aerospace Engineering Beijing Institute of TechnologyBeijing 100081China Institute of Computing Technology Chinese Academy of SciencesBeijing 100190China

In the realm of decision-making for defense and security applications,it is paramount to swiftly and accurately identify the intentions of incoming *** identification methods predominantly focus on single-target applications and overlook the perturbations introduced by measurement *** this study,we propose a novel concept:the Dynamic Distribution Probability(DDP)image,which is constructed using the estimated state and its covariance *** grayscale pixel value within the image signifies the probability of the presence of the agent within the *** proposed identification scheme integrates the use of Extended Kalman Filter(EKF),Convolutional Neural Network(CNN),Back Propagation(BP)network,and Gated Recurrent Unit(GRU)***,the DDP image is processed through a CNN to distill the formation characteristics,and the estimated swarm state from EKF is inputted into a BP network to deduce the kinematic *** outputs from both networks are summed and subsequently channeled into a GRU network to capture temporal *** numerical simulations and flight experiments are presented to demonstrate the robust anti-noise capability of the proposed scheme compared with conventional methods,as well as its superior training efficiency.

关键词： Intention Identification Kalman filters Convolutional neural networks Swarm intelligence

RNA病毒表达调控研究中的RNA结构分析技术

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病毒学报 2018年第4期34卷 579-585页

作者：王德亚于成明丁诚实原雪峰山东农业大学植物保护学院植物病理系泰安271018 枣庄学院生命科学学院枣庄277160

RNA病毒的多个生命过程如基因组复制、蛋白翻译等均需要特定RNA元件的调控,RNA结构是其发挥调控作用的分子基础,研究这些RNA元件结构及其在调控过程中的动态变化,将有助于深度解析相应过程的调控机制。RNA结构分析技术可以解析RNA元件... 详细信息

RNA病毒的多个生命过程如基因组复制、蛋白翻译等均需要特定RNA元件的调控,RNA结构是其发挥调控作用的分子基础,研究这些RNA元件结构及其在调控过程中的动态变化,将有助于深度解析相应过程的调控机制。RNA结构分析技术可以解析RNA元件的结构（二级结构和三级结构）。目前,RNA结构体分析技术主要有以下几种：Rnase酶法、In-line probing技术、SHAPE技术（Selective 2’-hydroxyl acylation analyzed by primer extension）、核磁共振技术、RNA结晶以及体内分析技术。本文将对RNA结构分析技术的发展历程、具体技术原理和操作细节、未来发展进行叙述,为相关科研工作提供技术参考。

关键词： RNA病毒表达调控 RNA结构